Ⅲ型前列腺炎患者血清和EPS中雌雄激素水平与勃起功能相关性分析

目的：探讨Ⅲ型前列腺炎（CP／CPPS）患者血清和前列腺按摩液（EPS）中雌二醇（E2）、睾酮（T）水平与勃起功能的关系。方法应用放射免疫法检测64例CP／CPPS患者和20例健康对照者血清、EPS中E2、T的水平,其中ⅢA组35例,ⅢB组29例,所有患者行前列腺炎症状指数（NIH‐CPSI）和勃起功能国际指数（IIEF‐5）评分。并按IIEF‐5分值分组,其中勃起功能障碍（ED）组32例,非ED组32例,比较各组数据。结果ⅢA组和ⅢB组血清、EPS中E2／T水平均高于对照组,差异有统计学意义（P＜0．05）。ⅢA组中ED患者20例（57．14％）多于ⅢB组12例（41．38％）,但差异无统计学意义（P＞0．05）。CP／CPPS组的IIEF‐5评分与血清、EPS中T水平呈正相关（r＝0．218、r＝0．231,P＜0．05）,与血清、EPS中E2／T呈负相关（r＝－0．189、r＝－0．652,P＜0．05）,与NIH‐CPSI评分无相关（P＞0．05）。ED组血清T（6．32±1．86）ng／mL低于非ED组（7．89±2．92）ng／mL和对照组（8．41±2．02）ng／mL;ED组EPS中E2／T（55．02±29．26）明显高于非ED组（14．06±9．36）和对照组（16．45±13．76）,差异有统计学意义（P＜0．05）。结论血清和EPS中雌雄激素失衡程度与CP／CPPS患者勃起功能相关。     

胃癌患者血清脂肪酸合酶表达水平的诊断意义

目的：探讨胃癌患者血清脂肪酸合酶（ FAS）表达水平的诊断意义。方法收集2012年1月-2013年12月在南京医科大学第二附属医院首次诊断为原发性胃癌的患者53例,其中Ⅰ和Ⅱ期患者24例,Ⅲ和Ⅳ期患者29例,并以51例来自健康体检的汉族人群与52例慢性浅表性胃炎的患者为对照,通过ELISA方法对研究对象的血清FAS表达水平进行定量检测。结果胃癌患者血清FAS的表达水平（5．25±0．48） ng/mL,明显高于健康对照组（2．82±0．28） ng/mL与慢性浅表性胃炎组（3．24±0．37）ng/mL,P＜0．05。通过ROC曲线分析,确定诊断胃癌的血清FAS最佳临界值为3．78 ng/mL。相较于Ⅰ和Ⅱ期患者FAS：（4．04±0．35） ng/mL,Ⅲ和Ⅳ期患者血清FAS （5．83±1．37） ng/mL表达水平更高（P＜0．05）。10例患者在手术前血清FAS水平＞3．78 ng/mL,其中有7例术后下降至＜3．78 ng/mL （P＜0．05）。结论血清FAS表达水平的升高与胃癌的发生、进展有关,对血清FAS含量的检测有助于胃癌的诊断与随访。     

1-磷酸鞘氨醇及脂联素在支架内再狭窄患者中的预测价值

目的：探讨1‐磷酸鞘氨醇（S1P）及脂联素在冠状动脉支架植入术后的预测价值。方法选择经复查冠脉造影后证实为支架内再狭窄患者50例为再狭窄组,选取同时期支架内无再狭窄者50例为对照组,收集研究对象的临床相关资料,分别检测其血清1‐磷酸鞘氨醇（S1P）、HDL‐S1P、脂联素及IL‐18水平,分析其与支架内再狭窄的相关性。结果与对照组比较,再狭窄组血清总S1P水平更低[（96．10±26．33）ng／mLvs．（113．40±32．72）ng／mL,P＜0．01],HDL‐S1P水平明显降低[（32．81±10．02）ng／mLvs．（42．72±11．75）ng／mL,P＜0．01],脂联素水平也明显降低[（7．38±2．11）mg／Lvs．（9．25±3．29）mg／L,P＜0．01],而两组间IL‐18水平则差异无统计学意义[（258．15±82．19）ng／Lvs．（224．98±84．15）ng／L,P＞0．05];脂联素与S1P（r＝0．712,P＜0．05）及HDL‐S1P（r＝0．821,P＜0．01）之间均呈正相关。结论S1P及脂联素是冠脉支架内再狭窄的独立预测因素,相对较高的S1P及脂联素浓度能降低经皮冠状动脉介入治疗（PCI）术后患者支架内再狭窄的风险。     

血清鸢尾素与2型糖尿病肾病的相关性研究

目的：探讨2型糖尿病（T2DM）患者血清鸢尾素（Irisin）与糖尿病肾病（DN）的相关性。方法选取新诊断的40例单纯T2DM患者（SDM组）、41例T2DM早期肾病患者（EDN组）、42例T2DM临床肾病患者（CDN组）,同时选取40例体检健康者为对照组（NC组）,采用酶联免疫吸附试验（ELISA）测定空腹血清Irisin水平。结果（1）4组血清Irisin水平由高到低依次为：NC组（320．48±107．03）ng／mL、SDM组（291．53±101．01）ng／mL、EDN 组（278．54±93．48）ng／mL、CDN组（209．12±88．53） ng／mL ,差异有统计学意义（P＜0．05）;（2）血清Irisin水平与胰岛素抵抗指数（HOMA‐IR）,空腹胰岛素（FINS）、空腹血糖（FPG）、糖化血红蛋白（HbA1c）、血肌酐（Scr）、尿素氮（BUN）、肿瘤坏死因子‐α（TNF‐α）水平及尿清蛋白／肌酐（UAlb／Cr）比值呈负相关（r值分别为－0．592、－0．610、－0．614、－0．638、－0．538、－0．552、－0．589、－0．561,P＜0．05）,与超氧化物歧化酶（SOD）、高密度脂蛋白（HDL）水平呈正相关（r值为0．578、0．556,P＜0．05）;（3）回归分析显示：HOMA‐IR、UAlb／Cr是Irisin的独立影响因素。结论 DN患者血清Irisin水平降低,血清Irisin与DN的严重程度相关。     

老年结直肠癌血清可溶性细胞间黏附分子-1及炎性因子表达的临床意义

目的：探讨血清可溶性细胞间黏附分子‐1（sICAM‐1）及炎性因子[C反应蛋白（CRP）、白细胞介素‐17（IL‐17）]在老年结直肠癌（CRC）患者中表达的临床意义。方法采用酶联免疫吸附测定（ELISA）对76例老年CRC患者（老年CRC组）及32例中青年CRC患者（中青年CRC组）手术前后外周血中sICAM‐1、CRP和IL‐17的表达水平进行检测,分析其与临床病理参数间的意义,并与60例健康体检者（对照组）比较。结果不同年龄CRC患者术前和术后血清sICAM‐1和IL‐17水平均高于对照组（P＜0．05）,术后二者水平较术前均明显降低（P＜0．05）。中青年CRC组术后及老年CRC组术前、术后CRP水平均与对照组,差异无统计学意义（P＞0．05）。中青年CRC组术前CRP水平高于对照组（P＜0．05）。中青年CRC组术前和术后血清sICAM‐1和IL‐17水平与老年CRC组比较,差异有统计学意义（P＜0．05）,而CRP水平两组,差异无统计学意义（P＞0．05）。老年CRC患者中sICAM‐1水平在分化程度、浸润深度、伴有淋巴结转移及TNM分期等方面组间差异有统计学意义（P＜0．05）;IL‐17水平在分化程度、浸润深度、伴有淋巴结转移、肿瘤大小及TNM分期等方面组间差异有统计学意义（P＜0．05）;CRP水平在浸润深度、伴有淋巴结转移及TNM 分期等方面组间差异有统计学意义（P＜0．05）。结论血清sICAM‐1、CRP和IL‐17水平在一定程度上反映了老年C RC的侵袭转移过程,在预测老年C RC患者发展及预后方面具有重要意义。     

血清CysC、β2-MG在妊娠期高血压疾病早期肾功能损伤中的意义

目的：探讨血清胱抑素（CysC）及血清β2‐微球蛋白（β2‐MG）在评估妊娠期高血压疾病（HDCP）患者早期肾功能损伤中的临床价值。方法采用全自动生化分析仪检测108例 HDCP 患者（妊娠期高血压组41例,轻度子痫前期组30例,重度子痫前期组37例）及孕周相近的健康单胎妊娠妇女（对照组40例）血清中 CysC 、β2‐MG 、肌酐（Cr）的水平。结果妊娠期高血压组、轻度子痫前期组、重度子痫前期组患者血清 CysC 、β2‐MG 水平均显著高于对照组（P＜0．05）;妊娠期高血压组、轻度子痫前期组、重度子痫前期组3组间血清 CysC 、β2‐MG 水平比较,差异均有统计学意义（妊娠期高血压组＜轻度子痫前期组＜重度子痫前期组,P＜0．05）。妊娠期高血压组、轻度子痫前期组和对照组之间血清 Cr 水平比较差异均无统计学意义（P＞0．05）,而重度子痫前期组与对照组之间血清 Cr 水平差异有统计学意义（P＜0．05）。相关分析结果显示 HDCP 患者血清 CysC 与β2‐MG 、Cr 呈正相关。结论联合检测血清 CysC 、β2‐MG 、Cr 对判断 HDCP 孕妇病情严重程度及诊断早期肾功能损伤具有重要的临床价值。     

快速康复外科在胰腺癌术后对血清IL-6、IL-10、TNF-α水平的影响

目的：探讨快速康复外科（FTS）在胰腺癌手术后对血清IL‐6、IL‐10及TNF‐α水平的影响及其临床意义。方法入选胰腺癌手术患者80例,分为验组40例和对照组40例,检测并比较两组手术前后不同时段血浆中各指标水平。结果（1）两组术前血清IL‐6、IL‐10、TNF‐α水平差异无统计学意义（P＞0．05）;试验组术后1、3d血清IL‐6水平高于术前,差异有统计学意义（P＜0．05）,而术后5d血清IL‐6水平与术前比较,差异无统计学意义（P＞0．05）;对照组术后3d血清IL‐6水平均高于术前（P＜0．05）;试验组术后同一时间段IL‐6水平均低于对照组（P＜0．05）。（2）试验组术后IL‐10水平均高于术前（P＜0．05）;对照组术后1、3d血清IL‐10水平高于术前（P＜0．05）;试验组术后同一时间段IL‐10水平均高于对照组（P＜0．05）;（3）试验组术后1d血清TNF‐α水平明显高于术前（P＜0．05）;术后3、5dTNF‐α水平与术前差异无统计学意义（P＞0．05）;对照组术后1、3d血清TNF‐α水平高于术前（P＜0．05）,而术后5d与术前差异无统计学意义（P＞0．05）;术后1、3d血清TNF‐α,试验组明显低于对照组（P＜0．05）。结论FTS疗法能明显降低胰腺癌术后的炎性反应,改善免疫抑制,加速患者术后康复。     

过敏性哮喘患者外周血细胞因子水平变化及临床意义

目的：探讨细胞因子IL‐17、IL‐22和IL‐10在过敏性哮喘患者外周血中的变化及临床意义。方法选取过敏性哮喘患者40例（哮喘组）和健康体检者30例（对照组）,采用ELISA法检测外周血培养上清中IL‐17、IL‐22和IL‐10的表达;电化学发光免疫分析法检测血清中Ig E的水平,全自动血液分析仪检测全血嗜酸性粒细胞比率;IL‐17、IL‐22和IL‐10与哮喘严重程度进行Pearson相关性分析。结果哮喘组血清中IgE的水平及全血嗜酸性粒细胞比率高于对照组（P＜0．05）;哮喘组外周血IL‐17、IL‐22水平高于对照组,而IL‐10水平低于对照组（P＜0．05）;哮喘严重程度与IL‐17、IL‐22水平呈正相关（P＜0．01）,而与IL‐10水平呈负相关（ P＜0．05）。结论 IL‐17、IL‐22和IL‐10在过敏性哮喘的发病机制中起重要作用,动态监测其变化有利于临床诊断及治疗。     

肝硬化患者血清中基质金属蛋白酶-3、 基质金属蛋白组织抑制因子-1 水平与肝硬化进程的研 究简

目的 探讨肝硬化患者血清中的基质金属蛋白酶-3（MMP-3）和基质金属蛋白组织抑制因子-1 （TIMP-1）值对于肝硬化进程的影响.方法 分析台州恩泽医疗集团路桥医院2011年1月～2012年6月收治的30例乙型肝炎后肝硬化代偿期患者（代偿组）及30例失代偿期患者（失代偿组）的临床资料,30名健康者作为正常对照组.采用BAS-ELISA法测定MMP-3的血清含量,同时采用ELISA双抗体夹心法测定TIMP-3的浓度;计算TIMP-1/MMP-3的比值.对比两组患者的MMP-3、TIMP-1的临床指标的异同.结果 ①代偿组患者血清MMP-3水平[（20.32±3.10） ng/mL]显著高于正常对照组血清MMP-3水平[（17.45±2.30）ng/mL],差异有统计学意义（P＜ 0.05）;代偿组患者血清TIMP-1水平[（1.72±0.40）ng/mL]显著高于正常对照组[（1.49±0.30）ng/mL],差异有统计学意义（P＜0.05）;代偿组TIMP-1/MMP-3值为（0.088±0.010）,比正常对照组TIMP-1/MMP-3值（0.086±0.010）有所升高,但差异无统计学意义（P＞0.05）.②失代偿组患者血清MMP-3水平[（22.73±5.20）ng/mL]显著高于正常对照组[（17.45±2.30）ng/mL],差异有统计学意义（P＜0.05）;失代偿组患者血清TIMP-1水平[（2.24±0.30）ng/mL]显著高于正常对照组[（1.49±0.30）ng/mL],差异有统计学意义（P＜0.05）;失代偿组患者TIMP-1/MMP-3值为（0.103±0.020）,显著高于正常对照组（0.086±0.010）,差异有统计学意义（P＜0.05）.③失代偿组患者血清中MMP-3水平显著高于代偿组患者,差异有统计学意义（P＜0.05）;失代偿组患者血清TIMP-1水平显著高于代偿组患者,差异有统计学意义（P＜0.05）;失代偿组患者TIMP-1/MMP-3值显著高于代偿组患者,差异有统计学意义（P＜ 0.05）.结论 代偿期肝硬化患者MMP-3以及TIMP-1皆显著升高,TIMP-1/MMP-3比值有升高趋势.失代偿期肝硬化患者MMP-3以及TIMP-1皆显著升高,TIMP-1/MMP-3比值显著升高.MMP-3/TIMP-1的比值与TIMP-1的表达与肝硬化程度有关.     

动脉粥样硬化血栓形成性脑卒中血清Lp-PLA2水平的研究

目的：探讨动脉粥样硬化血栓形成性（AT）脑卒中患者血清脂蛋白相关磷脂A2（Lp‐PLA2）水平的变化,并探讨其与脑梗死体积、神经功能缺损程度和早期预后之间的关系。方法选取于2014～2015年在河北省人民医院就诊的A T性脑卒中患者104例为研究对象（卒中组）,健康体检者100例作为对照组,比较两者Lp‐PLA2水平的差异;并采用Pullicino公式计算脑梗死体积,采用美国国立卫生研究所脑卒中量表（NIHSS）进行脑神经功能缺损程度评估,采用日常生活活动能力评定（Barthel）指数判断患者短期预后,比较不同脑卒中体积和不同脑神经损伤程度患者血清Lp‐PLA2水平变化,以及Lp‐PLA2水平对患者短期预后的影响。结果卒中组患者血清Lp‐PLA2水平高于对照组,差异有统计学意义（P＜0．05）,且与血清LDLC水平呈正相关（r＝0．859,P＜0．05）;卒中组患者血清Lp‐PLA2水平随脑梗死体积的增大而升高,随脑神经功能缺损程度的增加而升高,均呈正相关（r＝0．531、0．623,P＜0．05）,Lp‐PLA2水平高的患者短期预后较差（P＜0．05）。结论 AT 脑卒中患者Lp‐PLA2水平明显增高,可作为判断病情严重程度和短期预后的血清学指标。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.