肾上腺髓质素在人肾脏的免疫组织化学定位

肾上腺髓质素在人肾脏的免疫组织化学定位孙燕妮徐庆中一、材料和方法随机抽取无明显肾脏病变的尸体解剖的肾组织１０例，复查ＨＥ切片，取其蜡块作５μｍ厚切片用Ｓ－Ｐ法作免疫组化染色。简要方法如下：３％Ｈ２Ｏ２孵育５分钟，以消除内源性过氧化物酶；蒸馏水洗，Ｔｒ...     

表皮生长因子在成年猴脊髓的表达

目的探讨EGF(epidermal growth factor)在成年猴脊髓的表达。方法EGF特异性抗体的免疫细胞化学染色技术(SP法)。结果EGF免疫阳性物质主要位于神经元胞浆内，前角前内侧核、前角前核和前角前外侧核的大运动神经元以及背核的大神经元具有强免疫阳性信号，中等强度免疫阳性神经元主要分布于前角后内侧核、前角中央核、前角后外侧核和前角后外侧后核、中间内侧核和中央管周围，弱阳性神经元主要位于后角边缘核、胶状质、后角固有核和后角连合核。此外，在脊髓白质中可见部分EGF阳性胶质细胞和纤维。结论EGF免疫阳性反应产物在猴脊髓有广泛的分布，提示其功能可能涉及多种神经元和非神经细胞，为探讨表皮生长因子在猴脊髓分布规律和功能提供了有价值的形态学资料。     

增殖细胞核抗原在小鼠生后肾脏发育中的表达

目的：观察小鼠出生后肾脏发育过程中增殖细胞核抗原（PCNA）的表达特征,探讨出生后小鼠肾脏发育过程中细胞增殖的规律。方法：应用免疫组织化学技术检测小鼠出生后1、3、7、14、21、28和70d肾脏PCNA的表达。结果：小鼠出生后1～70d,皮质中的生肾区、肾小体、肾小管、髓放线以及髓质中的肾小管和集合管PCNA阳性细胞的表达具有一定的规律,早期PCNA阳性表达丰富,随着肾脏发育逐渐成熟而表达减弱直至消失。在70d成年小鼠肾脏中,没有检测到PCNA阳性细胞。结论：出生后小鼠肾脏皮质中的生肾区、肾小体、肾小管、髓放线以及髓质中小管的细胞增殖规律是由高逐渐降低的,直至成年完全停止。     

表皮生长因子及其受体在食管癌中的表达

目的 研究表皮生长因子（ＥＧＦ）及其受体（ＥＧＦＲ）在食管鳞状细胞癌中的表达。方法 用免疫组织化学ＡＢＣ法分析并研究染色结果与病理改变之间的关系。ＥＧＦ染色,以正常人颌下腺作为阳性对照。ＥＧＦＲ以正常人胎盘组织为阳性对照。ＥＧＦ和ＥＧＦＲ染色一抗的浓度分别为１：２００,１：１００,４℃孵育４８ｈ。结果 ＥＧＦ强阳性１８例,弱阳性１６例,ＥＧＦＲ强阳性１８例,弱阳性１６例。ＥＧＦ和ＥＧＦＲ的表达分为     

CCl_4 所致大鼠肝损伤自愈过程中颌下腺颗粒曲管细胞表皮生长因子的免疫组织化学研究

为探讨肝损伤后颌下腺颗粒曲管（ｇｒａｎｕｌａｒｃｏｎｖｏｌｕｔｅｄｔｕｂｕｌｅ，ＧＣＴ）细胞内表皮生长因子（ｅｐｉｄｅｒｍａｌｇｒｏｗｔｈｆａｃｔｏｒ，ＥＧＦ）的变化与肝的自愈过程之间的关系，本研究用免疫组织化学方法观察了ＣＣｌ４所致大鼠肝损伤自愈过程中颌下腺颗粒曲管细胞内表皮生长因子的反应强度及相关数量变化。结果显示：肝损伤后的第２、４、８ｄ，ＥＧＦ阳性细胞数增加（Ｐ＜０．０５，Ｐ＜０．０１，Ｐ＜０．０１）。肝损伤第２ｄ，ＧＣＴ细胞内弱阳性细胞数增多（Ｐ＜０．０１），第４ｄ，中等及弱阳性细胞数增多（Ｐ＜０．００１，Ｐ＜０．００１），至第８ｄ，强、中、弱阳性细胞数均增多（Ｐ＜０．００１，Ｐ＜０．００１，Ｐ＜０．０５）。结果表明：颌下腺ＧＣＴ细胞内的ＥＧＦ参与肝损伤后的修复及再生。     

CCl4所致大鼠肝损伤自愈过程中颌下腺颗粒曲管细胞表皮生长因子的…

为探讨肝损伤后颌下腺颗粒曲管（ＧＣＴ）细胞内表皮生长因子（ＥＧＦ）的变化与肝的自愈过程之间的关系，本研究用免疫组织化学方法观察了ＣＣｌ４所致大鼠肝损伤自愈过程中颌下腺颗粒曲管细胞内表皮生长因子的反应强度及相关数量变化。结果显示：肝损伤后的第２、４、８ｄ，ＥＧＦ阳性细胞数增加（Ｐ〈０．０５，Ｐ〈０．０１，Ｐ〈０．０１）。肝损伤第２ｄ，ＧＣＴ细胞内弱阳性细胞数增多（Ｐ〈０．０１），第４ｄ，中等及弱阳性     

人表皮生长因子的化学发光免疫分析法及其临床应用

本文建立的人表皮生长因子发光免疫法采用吖啶酯标记抗体，灵敏度可达２ｎｇｈＥＧＦ／ｍｌ，能满足尿中ｈＥＧＦ的测定。测定了正常人，孕妇及膀胱现人尿中ｈＥＧＤ的含量，孕妇尿中ｈＥＧＦ含量明显高于正常女性，膀胱癌患者尿中ｈＥＧＦ含量明显低于正常人。     

小鼠神经生长因子的细胞内免疫组织化学阳性反应定位

用免疫组织化学方法对小鼠神经组织和非神经组织中神经生长因子的分布进行了定位观察。实验结果表明 :(1)神经生长因子免疫反应主要局限于神经组织 ,尤其是神经元 ;非神经组织除颌下腺的颗粒曲管外基本上都呈阴性反应 ;(2 )在神经元 ,神经生长因子定位于细胞核与细胞质但以胞核为主 ,少量位于胞质靠近细胞膜的部位 ;(3 )雄性小鼠颌下腺颗粒曲管细胞的NGF主要定位于分泌颗粒的界膜区。神经生长因子在胞内定位的精确性和分布的多样性 ,为进一步研究其作用机制和信号传导提供了重要线索     

转化生长因子β1及其受体在着床期小鼠子宫内膜中的免疫组织化学研究

目的 进一步证实转化生长因子β１（ｔｒａｎｓｆｏｒｍｉｎｇ ｇｒｏｗｔｈ ｆａｃｔｏｒβ１,ＴＧＦβ１）在胚泡着床过程中的生物学作用。方法 利用昆明小鼠,在着床前后不同时期取材,通过改良的免疫组织化学方法,检测了ＴＧＦβ１及其受体（ＴＧＦＲ１）在子宫内膜中的表达状况。结果 受精后第４ｄ,子宫内膜表面上皮和腺上皮细胞中ＴＧＦβ１及其受体均较未孕期增加,成纤维细胞周围的细胞外基质（ｅｘｔｒａｃｅｌｌｕｌａｒ ｍａｔｒｉｘ,ＥＣＭ）也呈ＴＧＦβ１阳性着色。受精后第５、６ｄ,ＴＦＤβ１及其受体主要表达于初级蜕膜带（ｐｒｉｍａｒｙ ｄｅｃｉｄｕａｌ ｚｏｎｅ,ＰＤＺ）。随着胚泡植入的深入,ＴＧＦβ１及其受体广泛分布于蜕膜细胞。结论 ＴＧＦβ１及其受体的表达与胚泡植入过程密切相关。     

Effect of Recombinant Human Epidermal Growth Factor Against Cutaneous Scar Formation in Murine Full-thickness Wound Healing

A visible cutaneous scar develops from the excess formation of immature collagen in response to an inflammatory reaction. This study examined the role of epidermal growth factor (EGF) in the formation of cutaneous scars. Twenty Crl:CD-1 (ICR) mice were used and 2 full-thickness skin wounds were made on the dorsum of each mouse. One of the wounds was treated with recombinant human EGF by local application and the other was treated with saline for control until complete healing was achieved. The EGF-treated group''s wounds healed faster than the control group''s. The width of the scar was smaller by 30% and the area was smaller by 26% in the EGF-treated group. Inflammatory cell numbers were significantly lower in the EGF-treated group. The expression of transforming growth factor (TGF)-β₁ in the EGF-treated group was increased. It was observed that the amount of collagen in the EGF-treated group was larger than the control group. In the EGF-treated group, the visible external scars were less noticeable than that in the control group. These results suggest that EGF can reduce cutaneous scars by suppressing inflammatory reactions, decreasing expression of TGF-β₁, and mediating the formation of collagen.     

Immunohistochemical Demonstration of Epidermal Growth Factor Receptor and Ceruloplasmin in Thyroid Diseases

The expression of epidermal growth factor receptors (EGFR) and ceruloplasmin (CP) in thyroid diseases was investigated by immunohistochemical methods, and the results were compared with the expression of thyroglobulin (TG). Eighty eight surgical specimens of thyroid diseases, including follicular carcinoma (7 cases), papillary carcinoma (20 cases), follicular adenoma (29 cases), adenomatous goiter (10 cases), diffuse hyperplasia (20 cases) and chronic thyroiditis (2 cases), were studied. All cases of follicular carcinoma and 18 cases (90%) of papillary carcinoma expressed the EGFR immunoreaction in the cytoplasm with a moderate to strong staining intensity. A weak immunoreaction for EGFR was noted in some benign thyroid diseases. CP showed various degrees of positivity in all cases of follicular carcinoma and 19 cases (95%) of papillary carcinoma. The benign thyroid lesions were consistently negative for this antigen, not counting one case of Hiirthle cell adenoma. There was a positive correlation between EGFR and CP immunostaining intensity in thyroid carcinomas, representing higher expression of EGFR accompanied by a stronger staining intensity of CP. Except for two cases of papillary carcinoma, all cases showed immunoreaction for TG. The results indicate the enhanced expression of EGFR and CP in thyroid carcinomas. EGFR and CP thus appear to be valuable tools for differential diagnosis between benign and malignant thyroid neoplasms.     

Epidermal Growth Factor Receptor Mutations and the Clinical Outcome in Male Smokers with Squamous Cell Carcinoma of Lung

Epidermal growth factor receptor (EGFR) mutations in non-small cell lung cancer (NSCLC) have been reported to be related to certain clinical characteristics (i.e., female, non-smokers with adenocarcinoma) and gefitinib responsiveness. This exploratory analysis was performed to determine the incidence of EGFR mutations in male smokers with squamous cell carcinoma, who were treated with EGFR tyrosine kinase inhibitor, gefitinib. Sixty-nine Korean NSCLC patients were treated with gefitinib in a prospective study. For a subset of 20 male patients with squamous cell carcinoma and a history of smoking, pretreatment tumor tissue samples were obtained and analyzed for EGFR mutations (exons 18 to 21). EGFR mutations were found in 3 (15%) patients, including in-frame deletions within exon 19 (n=2) and L858R missence mutation in exon 21 (n=1). These 3 patients with EGFR mutations responded to gefitinib, whereas only one of remaining 17 patients with wild-type EGFR achieved clinical response. Trend toward longer progression-free (5.8 vs. 2.4 months; P=0.07) was noted in patients with EGFR mutations compared to those with wild-type EGFR. Although male smokers with squamous cell carcinoma have not been considered ideal candidates for gefitinib treatment, significant incidence of EGFR mutations was observed. The molecular markers should be considered to predict clinical benefits from gefitinib.     

CHO-EPO-EGFR细胞株的构建及在光固定EGF材料上的生长

采用人工细胞法 ( liposome)将两种质粒 p CEPO及 p RC/ CMV/ EGFR转染宿主细胞 -中国仓鼠卵巢细胞 ( CHO) ,得到表达水平稳定 ,形态良好的细胞株 CHO- EPO- EGFR,研究该细胞株在光固定EGF的聚苯乙烯材料上生长情况     

表皮生长因子在鼻窦炎患者鼻内镜手术后对术腔上皮化的影响

目的 观察对鼻窦炎患者进行鼻内镜手术后运用表皮生长因子对术腔上皮化的影响.方法 回顾性分析2013年11月至2015年11月期间于我院耳鼻喉科进行鼻内镜手术治疗的110名鼻窦炎患者的病历资料,将术后采用布地奈德混悬液常规治疗的60例患者设为对照组,常规治疗基础上加用重组人表皮生长因子治疗的50例患者设为观察组.分别比较治疗后两组患者临床治疗疗效、上皮化率、鼻腔粘膜Lund-Kennedy评分以及临床症状VAS评分.结果 观察组患者治疗总有效率为94.00%,显著高于对照组80.00%(P<0.05),差异具有统计学意义;观察组患者术后30d、3个月鼻腔粘膜Lund-Kennedy以及临床症状VAS评分显著低于对照组(P<0.05),术后14d、6个月差异无统计学意义(P>0.05);观察组患者术后1个月、术后3个月上皮化率显著高于对照组(P<0.05),术后6个月差异无统计学意义(P>0.05).结论在鼻窦炎患者鼻内镜手术后采用糖皮质激素喷雾剂的治疗基础上加用重组人表皮生长因子能有效缓解鼻窦炎症状,缩短上皮化时间,有着很高的临床价值.     

Epidermal growth factor receptor expression and growth fraction in human tumours of the nervous system

Summary 100 tumours of the human nervous system were investigated by means of immunohistochemistry in order to determine the expression of epidermal growth factor receptor (EGFr) and the proliferative activity as evaluated by demonstration of the proliferation-associated Ki-67 antigen. Epidermal growth factor receptor immunoreactivity was present in 79% (23/29) of the high-grade malignant gliomas examined but in only 9% (2/22) of the low-grade gliomas. Besides the gliomas, EGFr-expression was detectable in smaller amounts in most (13/15) meningiomas, in one anaplastic neurinoma and in individual tumour cells of one medulloblastoma. In addition, EGFr-expression was found in 50% (6/12) of metastatic carcinomas. Seven of eight medulloblastomas, two cerebral primitive neuroectodermal tumours (PNETs), three benign neurinomas, one ganglioneuroma, one metastatic intracerebral malignant melanoma, three spinal plasmocytomas and one immunocytoma showed no detectable EGFr-expression. Our results indicate that (1) the expression of EGFr in human tumours of the nervous system depends on the histological tumour type and (2) in the glioma group is related to the grade of malignancy. A close correlation between EGFrexpression and proliferative activity as evaluated by Ki-67 staining could not, however, be established.Supported by: Deutsche Forschungsgemeinschaft, SFB 200     

Expression,Content, and Localization of Insulin-Like Growth Factor I in Human Achilles Tendon

In animals insulin-like growth factor I (IGF-I) stimulates collagen production by fibroblasts and is expressed in tendons together with its binding protein 4 (IGFBP-4). However, the presence of IGF-I and IGFBP-4 in human tendon tissue is not described. Tissue IGF-I content was examined by immunoflourometric assay, real-time PCR, and immunohistochemistry used to localize and determine expression of IGF-I and IGFBP-4 in 6 postmortem human Achilles tendons. Tendon tissue concentrations of IGF-I were found to be 0.53 ± 0.10 ng/g. Furthermore, we demonstrated that IGF-I and IGFBP-4 are localized around the tendon fibroblasts and that mRNA for IGF-I and IGFBP-4 can be determined in human tendon tissue. The present study adds support for the roles of IGF-I and IGFBP-4 in the regulation of tendon adaptive responses to mechanical loading.     

神经生长因子在成年猴脊髓的表达--免疫组织化学研究

目的探讨神经生长因子(NGF)在成年猴脊髓的表达分布情况,为成年猴脊髓存在此生长因子的表达提供免疫组织化学证据。方法云南成年健康雄性猕猴5只,平均体重6kg左右,氯氨酮(0．25ml／kg)肌肉注射麻醉后,Zamboni液经心脏主动脉插管灌注固定动物,取T8脊髓节段入4％多聚甲醛后固定(4℃)6小时,在-20℃恒冷切片机下制作20μm厚的连续冰冻切片,间隔取片后分别以抗NGF抗体行免疫组化ABC染色。在Olympus光学显微镜下观察NGF在猴脊髓的表达分布情况。结果1．NGF的免疫阳性反应产物在成年猴脊髓腹角、背角神经元均有分布。2．NGF的免疫阳性反应产物在猴脊髓背角Li ssauer束有分布,表达NGF的Li ssauer束因染色而呈明显的毛刷状／发束状。3．NGF阳性神经膨体分布于猴脊髓背角Ⅰ、Ⅱ板层。4．NGF的免疫阳性反应产物在成年猴脊髓中央管(X板层)均有分布。结论成年猴脊髓存在NGF的表达,提示其可能在成年猴脊髓的生理过程中发挥作用。