闭合性胰腺损伤的诊治

分析１１例闭合性胰腺损伤的诊断和治疗特点。方法：对１１例闭合性胰腺损伤患者进行回顾性分析。结果；１１例患者１０例痊愈，１例因损伤严重，术后死于多器官功能衰竭。结论：通过讨论对提高闭合性胰腺损伤的诊断治疗水平，降低死亡率有很大帮助。     

19例闭合性胰腺损伤的诊断与外科治疗

目的 对闭合性胰腺损伤的诊断与外科治疗进行探讨。方法 对闭合性胰腺损伤按照Luacs分类法进行相应的外科治疗。结果 闭合性胰腺损伤的程度与外科治疗关系密切。结论 准确的判断胰腺损伤程度与正确的外科治疗是处理闭合性胰腺损伤的关键。     

闭合性胰腺损伤23例临床分析

目的 探讨闭合性胰腺损伤的治疗方法、临床疗效以及注意事项,提高闭合性胰腺损伤的治愈率.方法 对我院自2005年6月～2011年6月收诊的23例闭合性胰腺损伤患者的临床治疗资料进行回顾性的分析总结.结果 23例闭合性胰腺损伤患者中,有9例进行保守治疗,14例相应手术治疗,所有患者经过治疗后均痊愈出院,治愈率100%,术后有3例患者出现并发症,2例为上消化道出血,1例为切口感染,均给予相应处理痊愈.结论 闭合性胰腺损伤病情复杂,术前诊断比较困难,医务人员要提高对胰腺损伤的重视程度,综合多种临床症状以及检查结果,并根据胰腺损伤的程度以及合并损伤的脏器情况选择治疗方法和手术方式,避免误诊漏诊,提高闭合性胰腺损伤的治愈率.     

闭合性胰腺损伤的临床分析

目的：探讨闭合性胰腺损伤诊断和治疗的方法。方法：分析29例闭合性胰腺损伤的诊断、手术治疗和预后。结果：25例治愈，占8612％，死亡4例，占13．8％，发生胰瘘，假性囊肿在内的各类并发症8例，占27．6％。结论：CT、B超及血淀粉酶检查对胰腺损伤仍具有一定的诊断价值。术中关键是保持警觉，按胰腺损伤情况选择适当的术式。     

闭合性胰腺损伤的诊断与外科治疗(附22例病例分析)

闭合性胰腺损伤的诊断与外科治疗（附２２例病例分析）广东省增城市人民医院外科（５１１３００）徐润强在闭合性腹部损伤中，胰腺损伤较少见，占１％～３％［１］。这与它处于腹膜后，体积小有关。胰腺损伤多为合并伤，单纯的胰腺损伤更为少见。胰腺损伤的诊断、治疗较为...     

闭合性胰腺损伤的诊断与治疗

目的 探讨闭合性胰腺损伤的诊断和外科治疗方法。方法对我院收治的26例闭合性胰腺损伤患的临床资料进行回顾性分析。结果本组病人均经手术证实，轻度挫伤、挫裂伤、横断伤、胰头十二指肠损伤。术前诊断3例，其余23例均为剖腹探查时发现，分别行缝扎止血和外引流，远端胰腺切除、近端缝合和远端胰腺空肠吻合，十二指肠憩室化、胰十二指肠切除。本组发生胰瘘6例，死亡1例。结论闭合性胰腺损伤术前确诊困难，多数依靠术中探查发现，应根据胰腺损伤的部位和程度，选择恰当的手术方式。     

闭合性胰腺损伤在基层医院诊治体会

探讨胰腺闭合性损伤的临床特点、治疗措施、术后并发症处理.方法 我院近月来连续收治3例的诊治体会.结果 三例中治愈一例,胰漏一例,死亡一例.结论 B型超声和CT对胰腺闭合性损伤的诊断有一定价值治疗上,应根据胰腺损伤的部位程度性地选择手术方式.     

闭合性胰腺损伤治疗

目的:探讨闭合性胰腺损伤的治疗方法。方法回顾性分析32例闭合性胰腺损伤病例的临床资料。结果32例中,术前确诊18例,治愈32例发生并发症8例,术后继发出血和胰漏是主要并发症。结论闭合性胰腺损伤,术前诊断困难,应根据术中探查情况,采取简单而合理的手术方式是外科治疗的关键。     

闭合性胰腺损伤的诊断和外科治疗

目的 总结闭合性胰腺损伤的诊断和治疗经验.方法 对我院2002年12月～2010年9月收治的36例闭合性胰腺损伤的临床资料进行回顾性分析.结果 胰腺损伤情况,按美国创伤外科学会(AAST)胰腺损伤分级,Ⅰ级7例,Ⅱ级15例,Ⅲ级9例,Ⅳ级5例,无Ⅴ级病例.胰腺损伤术前诊断率40％.23例合并胰腺以外脏器损伤.23例行急诊剖腹探查术,其中17例针对胰腺损伤进行了手术治疗.所有病例均治愈,无死亡病例.结论 闭合性胰腺损伤的诊断需综合应用多种手段,外科手术是其主要治疗方法,非手术治疗应慎重.     

胰腺闭合性损伤21例手术治疗分析

目的探讨成人闭合性胰腺损伤的诊断和治疗方法。方法回顾性分析21例闭合性胰腺损伤病例的临床资料。结果21例中,术前确诊12例,手术Ⅰ期治愈10例,死亡2例;发生并发症9例。结论闭合性胰腺损伤术前诊断困难,术后并发症多,根据术中具体探查情况采取合理的手术方式是外科治疗成败的关键。     

Evaluation of diuretic and laxative activity of hydro-alcoholic extract of Desmostachya bipinnata （L,） Stapf in rats

OBJECTIVE： In continuation to the growing evidence for therapeutical potential of Desmostachya bipinnata （Linn） Stapf, the current pharmacological study was carried out to evaluate the diuretic and laxative activity of its hydro-alcoholic extract in rats. METHODS： The hydro-alcoholic extract of D. bipinnata whole plant was prepared by using Sox- hlet extractor and subjected to analysis by standard preliminary phytochemical tests. Evaluation of both diuretic and laxative activity was carried out using standard methods as reported earlier. Frusemide （20 mg/kg） was served as positive control for diuretic activity and sennosides （10 mg/kg） served as negative control for laxative activity. RESULTS： The hydro-alcoholic extract showed significant diuretic activity and was found to be the most potent in increasing the urinary output at 500 mg/kg when the effect was compared with that of the standard frusemide （P〈0.01）. Moreover, this extract was found to be most effective in increasing urinary electrolyte concentration （Na＋, K＋, and Cl-） at both doses tested. Whereas the results for laxative activity showed minimal increase of feces output at the dose of 500 mg/kg and the increase was negligible when compared with that of the standard drug sennosides. CONCLUSION： Altogether, the above significant findings validate and support its folkloric diuretic use and lend pharmacological credence to the ethno-medical use of this plant in traditional system of medicine, which demands further studies to investigate its active constituents, as well as its use and safety.     

UniPron is A Fully Effective Non-hormonal Reversible Contraceptive in Baboon Model （Papio Anubis）

Objective To determine the safety and efficacy of UniPron as a reversible contraceptive.
Methods Vaginal swabs were obtained before and after UniPron administration, cultured onto appropriate culture media and bacteria identification was done based on type of media used, Gram stain reactions, colony morphology and biochemical tests. Vaginal biopsy tissues were processed using paraffin wax method, stained with hematoxylin and eosin and examined under light microscopy to determine the effect of the product on vaginal tissues. The effect of UniPron on sperm was examined by mixing the product with electroejaculated spermatozoa in vitro at different concentrations. For efficacy studies, male baboons of proven fertility were mated with UniPron treated or untreated females of proven fertility during the fertile stages.
Results All the five females （100%） that were treated with UniPron did not conceive and they regained total fertility when the treatment was stopped while all the controls conceived. At a concentration of 40%, UniPron completely immobilized spermatozoa in an in-vitro system. UniPron mechanism of action was by lowering the vaginal pH and on application in baboon, the pH was lowered for at least 3 h after which it went back to normal.
Conclusions As we plan for a study to test UniPron as a microbicide to prevent STIs including HIV, our current study has established that this novel product is effective in contraception and harmless to vaginal tissues and vaginal microbial flora in a baboon model （Papio anubis）.     

Effect of the disruption of three cytoskeleton components on chondrocyte metabolism in rabbit knee cartilage

Background Chondrocytes＇ phenotype and biosynthesis of matrix are dependent on having an intact cytoskeletal structure.Microfilaments,microtubules,and intermediate filaments are three important components of the cytoskeletal structure of chondrocytes.The aims of this study were to determine and compare the effects of the disruption of these three cytoskeletal elements on the apoptosis and matrix synthesis by rabbit knee chondrocytes in vitro.Methods Chondrocytes were isolated from full-thickness knee cartilage of two-month-old rabbits using enzymatic methods （n=24）.The isolated cells were stabilized for three days and then exposed to low,medium,and high doses of chemical agents that disrupt the three principal cytoskeletal elements of interest：colchicine for microtubules,acrylamide for intermediate filaments,and cytochalasin D for actin microfilaments.A group of control cells were treated with carrier.Early apoptosis was assessed using the Annexin-FITC binding assay by flow cytometry on days 1 and 2 after exposure to the disrupting chemical agents.The components and distribution of the cytoskeleton within the cells were analyzed by laser scanning confocal microscopy （LSCM） with immunofluorescence staining on day 3.The mRNA levels of aggrecan （AGG） and type Ⅱ collagen （Col-2） and their levels in culture medium were analyzed using real-time PCR and enzymelinked immunosorbent serologic assay （ELISA） on days 3,6,and 9.Results In the initial drug-dose-response study,there was no significant difference in the vitality of cells treated with 0.1 μmol/L colchicine,2.5 mmol/L acrylamide,and 10 μg/L cytochalasin D for two days when compared with the control group of cells.The concentrations of colchicine and acrylamide treatment selected above significantly decreased the number of viable cells over the nine-day culture and disrupted significantly more cell nuclei.Real-time PCR and ELISA results showed that the mRNA levels and medium concentrations of AGG and Col-2 were significantly decreased for     

Liuwei Dihuang,a traditional Chinese herbal formula,suppresses chronic inflammation and oxidative stress in obese rats

OBJECTIVE： To investigate the anti-inflammatory, anti-oxidative stress, and adipokine-ameliorating effects of Liuwei Dihuang （LWDH）, a traditional Chinese herbal formula, in obese rats. METHODS： After 2 weeks of acclimation with free access to regular rodent chow and water, obese-prone-caesarean-derived （OP-CD） rats were fed a modified AIN-93G diet containing 60% energy from fat. Treatment was performed twice daily by gavage feeding with 500, 1 500, or 3 500 mg/kg body weight LWDH suspended in water （n=12 rats per group）. Twelve obese-resistant-CD （OR-CD） rats were fed the atherogenic diet and gavaged with water, and served as the normal control. Blood biomarkers of inflammation, oxidative stress and adiponectin were measured post-sacrifice and used to determine the treatment effect of LWDH and assess the suitability of OR/OP-CD rats for studying these parameters. RESULTS： After 9 weeks of treatment, LWDH lowered serum C-reactive protein （CRP） and tumour necrosis factor-α （TNF-α） levels. Serum interleukin-6 （IL-6） levels showed a tendency towards reduction, but were not significantly different from the OP-CD control. Liver superoxide dismutase （SOD） activity was increased in response to all three doses of LWDH, while the levels of reduced （GSH） and oxidized glutathione （GSSG） and thiobarbituric acid reactive substances （TBARS） were unchanged. Serum adiponectin levels were increased in response to oral administration of LWDH at the dose of either 500 or 1 500 mg/kg body weight. In addition, comparisons between OR-CD and OP-CD rats revealed differential, and for some biomarkers, conflicting characteristics of high-fat diet-fed OP-CD rats in reference to obese human subjects in terms of inflammatory and oxidative stress biomarkers and circulating adiponectin levels. CONCLUSION： The results show, for the first time, the anti-inflammatory, anti-oxidative stress and adiponectin-ameliorating effects of LWDH in obese rats. The suitability of the OP-JOP-CD rat model as     

New Mechanism of Herb-herb Interaction between Ginseng and Trogopterus Based on CYPs in Rat Livers

The study was designed to investigate the potential mechanism of herb-herb interaction between ginseng and Trogopterus （Trg） based on Cytochrome P450 isozymes （CYPs） in rat livers. We estimated the influence on CYP1A2, CYP2E1, and CYP3A1/2 activity caused by ginseng and Trg used in combination. The CYP1A2 and CYP2E1 enzyme activity were induced by ginseng and Trg used in combination. And this induction effect was caused via inducing CYP1A2 and CYP2E1 protein expression which was supposed caused by inducing the gene expression of CYP1A2 and CYP2E1.     

益智健脑颗粒联合针灸对阿尔茨海默病大鼠学习记忆的影响

目的观察益智健脑颗粒联合针灸对阿尔茨海默病（Alzheimers disease,AD）大鼠学习记忆的影响。方法将大鼠随机分为假手术组（A组）、模型组（B组）、针灸组（C组）、益智＋针灸组（D组）各10只,B、C、D 3组分别以海马CA1区注射β淀粉样蛋白25-35（Aβ25-35）造模,A组注射等量的双蒸水,各组分别治疗20 d后行Morris水迷宫试验,观察大鼠学习记忆能力变化。结果B组较A组的平均潜伏期明显延长,差异具有统计学意义（P〈0.05）;与B组比较,C组、D组的平均潜伏期明显缩短,过台次数增多,差异具有统计学意义（P〈0.05,P〈0.01）;与C组比较,D组的潜伏期缩短,过台次数增多,差异具有统计学意义（P〈0.05）。结论益智健脑颗粒联合针灸能够提高Aβ25-35介导的AD模型大鼠的学习记忆能力。     

The Targeted Pro-apoptotic Effect of scFv-mediated Delivery of Caspase-6 on HER2-positive Osteosarcoma Cells

Objective To investigate the pro-apoptotic effect of scFv-mediated delivery of caspase-6 fusion protein on osteosarcoma E 10 cells. Methods The expression of human epidermal growth factor receptor-2 （Her-2） in osteosarcoma cell line E10 was examined by flow cytometry analysis. ScFv-mediated delivery of caspase-6 was generated by sequential fusion of the genes of a signal peptide, a single-chain Her-2 antibody （e23sFv）, a PEA translocation domain （PEA aa253-264）, and an recombinant caspase-6. The pCMV-immunocaspase-6 was produced by cloning above mentioned fusion genes into pCMV plasmid, and then pCMV-immunocaspase-6 plasmid mixed cationic liposome was transfected into E10 cells. The expression of fusion gene on transfected E10 cells was detected by immunocytochemical staining, The pro-apoptotic effects of fusion gene expression on morphology and growth status of E10 cells were observed by trypan blue cell staining, electron microscopy and MTT assay. Results The fusion protein was detected in the cytoplasm of the transfected E10 cells. These cells presented typical characteristics of apoptosis as detected by electron microscopy. MTT assay revealed that the proliferation of transfected E10 cells was suppressed than that of non- or mock-transfected cells （P〈0.01）. Conclusion ScFv-mediated delivery of caspase-6 fusion protein can induce apoptosis of Her-2 positive osteosarcoma E10 cells, suggesting the potential of this strategy for the treatment of human high-grade osteosarcoma.     

WHO西太区与“世界中医药学会联合会”中医名词术语国际标准比较研究：精、神、气、血、津、液部分

精、神、气、血、津、液是中医理论中6个非常重要的概念，有关术语在《WHO西太区传统医学国际标准名词术语》中一共收录了59条，“世界中医药学会联合会”（以下简称世中联）《中医基本名词术语中英对照国际标准》中收录了58条。血、津、液的内涵较为具体，有一定的物质基础，理解并不困难。翻译上虽有差异，但亦不难统一。精、神、     

表皮生长因子受体酪氨酸激酶抑制剂在非小细胞肺癌应用的瓶颈——获得性耐药

以表皮生长因子受体酪氨酸激酶抑制剂（epidermal growth factor receptor-tyrosine kinase inhibitor,EGFR-TKI）分子靶向治疗研究为肇始,转化性研究使基础实验和临床实践间的鸿沟迅速填平,改变着人们认识治疗肺癌的视角。无可否认,EGFR-TKI上市后极大地延伸了肿瘤学家治疗肺癌的手段,但不管从临床经验、临床研究数据、分子生物学层面还是文献计量学的角度,     

Iloprost inhibits fracture repair in rats

Background Previous studies have shown that prostaglandins （PGs） dramatically stimulate healing processes in bone.However,the effect of prostaglandin l2 （PGI2） on fracture healing remains unclear.To investigate the effect of PGI2,a study on fracture healing process in closed tibia fractures was designed.Methods Thirty-six Sprague-Dawley male rats were randomized into two groups.On the first day,their right tibias were fractured by three-point bending technique.The study group （n=18） received a single injection of 10 μg/kg iloprost for 5 days,while the control group （n=18） received saline solution in the same way.On the 7th,14th and 28th days following the fracture,six rats were sacrificed and their right legs were harvested in each group.The progression of fracture healing was assessed for each specimen by the scores of radiography （by Lane-Sandhu） and histology （by Huo et al）.Results On the 7th day,the radiographic and histologic scores were equal.On the 14th day radiographic total score was 6 and histologic total score was 23 in the iloprost group,whereas radiographic total score was 11 and histologic total score was 33 in the control group.On the 14th day radiographic and histologic scores were significantly decreased in the iloprost group compared to the control group （P 〈0.05）.On the 28th day radiographic total score was 12 and histologic total score was 37 in the iloprost group,whereas radiographic total score was 15 and histologic total score was 40 in the control group.On the 28th day although there was a decrease in radiographic and histologic scores of the iloprost group acording to control group,it was not statistically significant （P 〉0.05）.Conclusion Iloprost delays fracture healing in early stage in rats.