大肠乳头状肿瘤AnNOR的观察与探讨

对８７例大肠乳头状肿瘤（腺瘤１９例，腺瘤伴不典型增生８例，腺癌６０例）行核仁组成区相关嗜银蛋白（ＡｇＮＯＲ）观察分析。提示，ＡｇＮＯＲ随细胞增生的程度而增加，在良恶性病变之间有显著差异（Ｐ〈０．００１）。因此，对病理学诊断和科研为一有用手段。     

大肠肿瘤P0CNA、AgNOR、DNA及核形态定量研究

目的：研究大肠上皮异型增生多指标多参数检测及其在大肠肿瘤的计算机自动化诊断中的应用价值。方法：本课题应用计算机图像分析技术对53例大肠癌、30例大肠腺瘤及10例正常肠粘膜进行DNA含量、核形态参数、AgNOR的计数、颗粒形态参数及PCNA等多指标多参数检测。结果：多数参数在各组间具有显著性差异（P＜0．01）,经判别筛选,DNA指数、AgNOR的平均颗粒数、阳性率、平均光密度、颗粒表面因子,PCNA的平均光密度、阳性率,核的形态因子、 核宽径及平均光密度等10个参数判别能力较大,为最佳判别参数。参数值大多随着正常、腺瘤及腺癌顺序递增,显示这些参数均能较好反映肿瘤的演进过程。PCNA大部分参数值在肿瘤的演进过程 中其高峰出现在腺瘤阶段,故其在大肠癌早期诊断中的价值需进一步探讨。结论：应用图像分析技术对大肠癌及癌前病变进行多指标多参数的计量分析,具有鉴别诊断有重要意义,为大肠肿瘤的自动化诊断提供客观依据。     

血管肿瘤的AgNOR检测

血管肿瘤的ＡｇＮＯＲ检测康永禄，沈玮，吕纯义天津市天和医院（天津市３０００５０）近年来ＡｇＮＯＲ染色技术广泛运用于上皮性肿瘤良、恶性的判断，体液内肿瘤和非肿瘤细胞的鉴别以及淋巴瘤的辅助诊断等，但未发现对血管肿瘤进行ＡｇＮＯＲ检测的研究报道。本文通过对...     

大肠肿瘤核仁组成区及自动图像分析

核仁组成区(Nucleolar Organizer Re-gion,NOR)及相关嗜银蛋白(AgNOR)用于良恶性肿瘤鉴别诊断的研究,是近期兴起的一种新技术,但目前大都采用人工肉眼观察方法,有一定主观性.我们用此种技术对大肠肿瘤进行了人工观察及自动图像分析研究,结果报道如下.选大肠癌、腺瘤及正常粘膜(肠癌标本远端粘膜)各30例.肠癌组含高分化腺癌10例,中分化腺癌7例,低分化腺癌2例,乳头状腺癌3例,粘液腺癌4例,印戒细胞癌3例,未分化癌1例.常规石蜡制片,经Ploton胶银一步法染色.     

东亚钳蝎毒灌肠治疗大肠癌后肿瘤组织中AgNOR变化

40例直场癌用东亚钳蝎毒泡汤治疗前后，正常组织、癌近分组织和肿瘤组织中AgNOR颗粒数和AgNOR形态分型的变化，结果发现：治疗后癌近旁组织和肿瘤组织中AgNOR颗粒数较治疗前明显减少，治疗后与治疗前比较差异有显著性（P＜0．01）．治疗前肿瘤组织中AgNOR＜4．0／核计27例，占67．5％；≥4．0／核计13例，占32．5％；而治疗后肿瘤组织中AgNOR＜4．0／核计37例，占92．5％，≥4．0／核计3例，占7．5％．而AgNOR颗粒形态学分型结果显示：治疗前肿瘤组织单一型和核仁内型7例，占17．5％，聚集型和弥散型33例，占82．5％；治疗后，单一型和核仁内型23例，占57．5％，聚集型和弥散型17例，占42．5％．肿瘤组织AgNOR颗粒形态学分型治疗后与治疗前比较，由聚集型和弥散型向单一型和核仁内到转变．治疗结果表明，东亚钳蝎毒对大肠癌有明显的抑杀作用．     

AgNOR在肿瘤研究中应用的国内概况

截止1991年初,作者收集了国内公开发表的有关AgNOR(核仁组成区嗜银蛋白)在肿瘤研究应用的文章四十余篇,这些文章大致可以反映国内该方面研究的概况。作者仅对此扼要地综述,以供参考。一、文献资料的发表情况1988年以前,有关AgNOR的文章较少,且主要在于肿瘤遗传学方面的研究。而在肿瘤病理学上的探讨则从1989年开始刊登仅6篇,1990年增至14篇,而到1991年初则已见12篇。此方面所发表的文章中综述占两篇,技术方面的文章3篇,消化系统病变10篇,皮肤粘膜病变6篇,乳     

大肠肿瘤组织中人乳头状瘤病毒感染与P53基因表达的研究

目的探讨HPV16和HPV18感染及P53基因异常表达与大肠肿瘤发生间的关系。方法采用地高辛标记的HPV16和HPV18DNA深外分别在40例大肠癌和30例大肠腺瘤组织石蜡切片上进行原位杂交探测HPVDNA。同时,采用SABC法检测大肠癌和大肠腺瘤的P53表达水平。结果受检大肠腺瘤组织HPVDNA阳性8例（27％）,其中HPV16DNA5例,HPV18DNA3例,主要见于管状绒毛状腺癌和绒毛状腺瘤;大肠腺癌组织中HPVDNA阳性19例（4％）,其中HPV16DNA14例,HPV18DNA5例。HPVDNA主要见于肿瘤细胞核中,少部分见于胞浆中。大肠癌组织中HPV16．18DNA检出阳性率明显高于大肠腺癌,且腺痛中HPV16DNA阳性率明显高于其他类型腺瘤。大肠癌P53蛋白阳性率为48％;腺癌的阳性率则为16．2％。结论HPV16．18型感染并整合至宿主细胞DNA中可能导致P53基因突变与大肠腺癌发生有密切关系。     

各型大肠息肉灶佳异型增生的形态学与组化特征

作者对７６例大肠各型息肉和腺瘤进行了组织形态学观察，对所见灶性异型增生的特征及其意义进行了分析。结果显示，大肠各型息肉灶性异型增生的检出率分别为：炎性息肉１０％，幼年性息肉为零，增生性息肉８．３％，息肉状腺瘤４２．９％，绒毛状腺瘤６６．７％，家族性腺瘤５０％和腺瘤癌变１００％。灶性异型增生细胞位于某一腺管或分支的乳头上，少者仅有２至５个细胞，多者达１０余个细胞。可向管腔内或基底膜外突起，拥挤排列，     

甲状腺滤泡性肿瘤DNA含量与AgNOR计数研究

目的：探讨ＤＮＡ含量与ＡｇＮＯＲ对甲状腺良恶性滤泡性肿瘤的鉴别诊断价值。方法：应用流式细胞术和胶体银染技术对９例正常甲状腺组织和３６例滤泡生肿瘤（２２例腺瘤和１４例腺癌）进行ＤＮＡ含量分析和ＡｇＮＯＲ计数。结果：１４例滤泡性癌中，１１例为ＤＮＡ异倍体，而２２例滤泡性腺瘤仅１例为ＤＮＡ异倍体，且伴有不典型增生。ＡｇＮＯＲ由正常甲状腺组织、滤泡性腺瘤至滤泡性癌逐渐增加，相互之间有显著性差异（Ｐ〈０．０     

肺未分化癌的核仁形成区嗜银蛋白实验研究

作者对２０例未分化型肺癌与１０例正常淋巴细胞（２例淋巴结，８例阑尾组织的正常淋巴细胞）进行了核仁形成区嗜银蛋白（ＡｇＮＯＲ）实验研究，结果表明，两者之间的ＡｇＮＯＲ颗粒数目有显著性差异（Ｐ〈０．０１），颗粒的大小分布也明显不同，提示此法对诊断和鉴别诊断未分化肺癌与正常淋巴细胞有重要意义。     

乳腺肿瘤细胞TrfR表达与AgNOR计数的相互关系及临床应用价值

收集57例乳腺肿块穿刺或印片标本,用PAP法进行免疫化学染色观察TrfR阳性细胞和核仁组成区嗜银蛋白染色计算AgNORs颗粒数。结果提示:乳腺癌与非癌组织之间OKT_9,及AgNORs数的差别非常显著(P<0.01),而乳腺纤维腺瘤与腺病其差别无意义(P>0.05),OKT_9阳性与阴性乳腺癌之间AgNORs数的差别是显著的(P<0.05),呈正比关系。我们认为AgNORs和TrfR检测,对判断乳腺细胞其良恶性有十分重要意义,能作为细胞穿刺的辅助诊断手段。     

上颌窦鳞状上皮良、恶性病变的Ag-NORs研究

采用银染技术，对84例上颌窦良、恶性病变标本进行了Ag－NORs研究。结果显示：上颌窦乳头状瘤及不典型增生的Ag－NORs计数明显高于单纯增生及正常粘膜，乳头状瘤癌变及鳞癌Ag－NORs计数又高于乳头状瘤及不典型增生（P＜0．05）。乳头状瘤与不典型增生间Ag－NORs计数无明显差别（二者都应视为癌前病变），乳头状瘤癌变与鳞癌间Ag－NORs计数也未见明显差别（P＞0．05）。鳞癌Ⅰ、Ⅱ级间以及Ⅰ、Ⅲ级间Ag－CORs计数差别显著（P＜0．05），Ⅱ、Ⅲ级间则无明显差别（P＞0．05）。结果表明，Ag-NORs计数在上颌窦良、恶性病变的鉴别及检测癌前病变上有一定价值，可作为上颌窦肿瘤的一个辅助诊断指标。     

乳腺癌核仁组成区相关嗜银蛋白的研究

本文应用核仁组成区相关嗜银蛋白（ＡｇＮＯＲ）当色技术，对５０例乳腺活检标本进行研究。其中包括正常乳腺组织，乳腺良性肿瘤和乳腺癌。结果表明，ＡｇＮＯＲ颗粒数量、大小及分布情况在乳腺良、恶性病变之间均有差异，特别是核内ＡｇＮＯＲ颗粒的平均数量改变更为明显。因此，该技术用于乳良、恶病变的诊断，具有重要的辅助意义。     

AgNOR变化在移行上皮肿瘤中的诊断意义

应用 AgNOR 染色技术对32例泌尿系移行上皮及其肿瘤组织细胞核内 AgNOR 的定量研究表明,正常移行上皮与移行上皮乳头状瘤之间 AgNOR 差异无显著性(P>0.05),而移行上皮乳头状瘤、不同恶性程度的移行上皮癌各组间 AgNOR 比较,差异均有显著性(0.01相似文献   

Changes in Histologic Grade and Argyrophilic Nucleolar Organizer Regions during Progression of Prostate Cancer

To determine the changes in histologic features during the courseof prostate cancer under longterm endocrine therapy, histologicgrade and argyrophilic nucleolar organizer regions (AgNORs)were examined in specimens before treatment, at relapse, andat cancer death. A total of 29 patients who had received endocrinetherapy and died of prostate cancer were evaluated. Among the29 cases, biopsy tissues before treatment (25 cases) and duringprogression from endocrine therapy (10 cases) were comparedwith autopsy specimens. Histologic grade was determined by themethod of Gleason, and the number of AgNORs in cancer cellswas counted. Survival of the patients was compared with thehistologic features. There was a tendency for a higher gradeof cancer during the clinical course. Moreover, a statisticallysignificant increase in the number of AgNORs was observed frompretreatment biopsy to autopsy. Upon comparison of metastaticsites with local cancer at autopsy, no significant differencewas noticed in terms of histologic grade or AgNOR count Althoughthere was no correlation between the number of AgNORs and survivalafter initial treatment, an inverse relationship was demonstratedbetween the number of AgNORs and survival in patients with systemicprogression after endocrine therapy. In conclusion, prostatecancer shows an increase of malignant potential, as assessedby histologic grade and the number of AgNORs. Patients withcancer of high proliferative ability showing high grade andgreater numbers of AgNORs have poorer prognosis from progression.     

Nucleolar organizer region count and subjective AgNOR pattern assessment (SAPA) score in skin tumors.

OBJECTIVE: To study the argyrophilic nucleolar organizer region (AgNOR) count and subjective AgNOR pattern assessment (SAPA) score in cytologic and histologic specimens of various skin tumors. STUDY DESIGN: The study group consisted of 37 patients (14 benign and 23 malignant) of various skin tumors. In all cases, cytology by fine needle aspiration cytology (FNAC), and histological specimens were studied by conventional staining and silver staining for AgNOR. RESULTS: The mean count in benign tumors in cytologic specimens was 2.08 +/- 0.01, compared with 5.50 +/- 1.12 in malignant tumors (P<0.001). In histologic specimens, mean count was 2.13 +/- 0.51 in benign, compared with 5.38 +/- 1.10 in malignant tumors (P<0.001). The SAPA score in benign tumors (P<0.001) in cytologic specimens, was 6.07 +/- 0.83, compared with 10.65 +/- 1.27 in malignant tumors, and in histology, it was 6.07 +/- 0.87 in benign, compared with 10.83 +/- 1.15 in malignant tumors (P < 0.001). Melanoma showed the higher AgNOR count compared with squamous cell carcinoma and basal cell carcinoma. The parameters were statistically significant between the grade of tumor in squamous cell carcinoma and the positivity of lymph nodes as demonstrated by SAPA score. No correlation was found between the clinical stage and Clark level of melanoma. Although, AgNOR count and SAPA score showed similar results, the indicators of validity were higher in SAPA than AgNOR count. CONCLUSION: Although, AgNOR count and SAPA score gave similar results, but the indicators of validity were higher in SAPA score than AgNOR count.     

桂皮酸对PGCL3人肺癌细胞增殖和核仁组成区的影响

本研究的目的是探讨低毒性的食品调味剂桂皮酸对ＰＧＣＬ３人肺癌细胞的抑制作用。研究结果发现１ｍｍｏｌ／Ｌ和３ｍｍｏｌ／Ｌ桂皮酸能使ＰＧＣＬ３人肺癌细胞增殖受到明显抑制（Ｐ＜０．０５，Ｐ＜０．０１），增殖抑制率达４８．６％，同时发现细胞的核仁形成率明显下降，每个细胞平均核仁数由２．９２个降至１．６６和１．９６个（Ｐ＜０．０１），核仁内核仁组成区嗜银蛋白（ＡｇＮＯＲ）颗粒明显减少，每个细胞核仁内ＡｇＮＯＲ颗粒数由３９．２６±７．４９降至９．５４±４．６１和１９．６８±１０．４８（Ｐ＜０．００１和Ｐ＜０．０１）。表明桂皮酸是ＰＧＣＬ３人肺癌细胞有效的抑制剂，在抗癌作用方面具有潜在的应用价值。     

口腔颌面部肿瘤患者淋巴细胞银染核仁形成区的观察

本实验采用银染法.对口腔颌面部恶性肿瘤患者、良性肿瘤患者及正常人外周血淋巴细胞的银染核仁形成区变化进行观察。结果表明恶性肿瘤组患者淋巴细胞Ag-NOR、Ag-AAC和Ag-AA频率均高于良性肿瘤组和对照组(P<0.005)。良性肿瘤组与对照组间以及早、晚期恶性肿瘤患者之间Ag-NOR、Ag-AAC和Ag-AA频率差异均无显著意义(P>0.05)。提示口腔领面部恶性肿瘤的发生,与患者染色体上rRNA基因数量增加及rRNA基因活性提高有关。     

Comparative Immunohistochemical Studies of p53 and Proliferating Cell Nuclear Antigen Expression and Argyrophilic Nucleolar Organizer Regions in Pancreatic Duct Cell Carcinomas

Thirty-eight cases of pancreatic duct cell carcinoma were examined for p53 expression and proliferating cell nuclear antigen (PCNA) by enhanced immunohistochemistry, as well as for changes in numbers of argyrophilic nucleolar organizer regions (AgNORs). Fifteen cases (39.5%) showed p53 overexpression, which tended to increase in proportion to the histopathological grading of malignancy. However, tumor stage and lymph node status were not correlated to p53 overexpression. PCNA labeling index (LI) increased with both histologically malignant grading and pathological stage, but was not correlated with lymph node status. The expressions of p53 and PCNA thus did not necessarily reflect the degree of malignant development. In contrast, AgNOR number showed statistically significant correlations with these three indicators of malignancy. A comparative analysis of p53, PCNA LI and AgNOR number showed overexpression of p53 to be correlated to PCNA LI and essentially unrelated to AgNOR number. The present results thus indicate a close relation between p53 and PCNA, while AgNORs appear to be regulated separately from either of them.