Erythrocyte adenylate kinase isoenzyme as a marker for hemolysis

The presence in serum of adenylate kinase isoenzyme originating from erythrocyte can be useful as a marker for detecting hemolysis. We have presented preliminary evidence for identifying hemolytic anemia patients earlier by determining erythrocyte AK isoenzyme activity in serum (or plasma) rather than using measurement of plasma hemoglobin concentration. This test being quite specific for hemolysis should find use as a quick method for estimating the extent of in vivo hemolysis in hemolytic patients earlier than heretofore possible. J. Clin. Lab. Anal. 11:351–356, 1997. © 1997 Wiley-Liss, Inc.     

肿瘤M2型丙酮酸激酶在乳腺癌诊断中的临床价值

目的评价肿瘤M2型丙酮酸激酶(Tumor M2-PK)在乳腺癌诊断中的临床应用价值。方法采用酶联免疫吸附法检测70例乳腺癌患者及50例乳腺良性疾病患者外周血中Tumor M2-PK的含量。结果乳腺癌患者血浆Tumor M2-PK水平明显高于乳腺良性疾病患者,差异有统计学意义(P<0.01)。乳腺癌患者Tumor M2-PK水平与TNM分期密切相关,TNM分期越高,Tumor M2-PK水平也越高。淋巴结转移患者Tumor M2-PK水平显著高于未转移者,差异有统计学意义(P<0.01)。应用受试者工作特征(ROC)曲线,Tumor M2-PK检测乳腺癌的最佳临界值为13.3,其诊断乳腺癌的敏感性为67.1%,特异性为80.0%,阳性预测值为82.5%,阴性预测值为63.5%,准确性为72.5%。结论 Tumor M2-PK可作为乳腺癌的一个较好的诊断标志物,并对乳腺癌的临床分期、浸润转移的判断有一定的意义。     

血清胸腺嘧啶核苷激酶的检测及临床意义

利用抗胸腺嘧啶核苷激酶 (TK)单克隆抗体 ,建立增强化学发光法 (ECLA) ,检测血清中的TK。在肿瘤病人中 ,肺癌的TK阳性率为 91 5 % ,直肠癌为 90 0 % ,胃癌为 90 6 % ,急性淋巴细胞性白血病 (ALL)、急非淋白血病 (ANLL)、淋巴瘤分别为 93 3% ,6 5 0 %和 91 6 % ;非肿瘤病人TK的阳性率为 4 2 %。 2 0 7例体检健康人群血清均为阴性 ,TK含量明显低于各肿瘤组病人 (P <0 0 0 1) ,而与非肿瘤病人无显著差异 (P >0 0 5 ) ;胃癌和直肠癌病人的TK阳性率明显高于癌胚抗原(CEA)的阳性检出率 (P <0 0 0 5 )。结论 :血清TK含量大于 5pmol/L可判定为异常 ;ECLA检测TK对ALL、淋巴瘤、肺癌、胃癌和直肠癌等有较高的诊断效果 ,将会成为肿瘤筛查、恶性程度和预后观察的工具。而且本法具有特异性好 ,结果易于观察且可长期保存的优点。     

Serum uric acid levels: a useful but not absolute marker of compliance with fenofibrate treatment

The purpose of our study was to investigate whether measurement of serum uric acid levels is a reliable method to assess adherence to fenofibrate treatment. This was a 21 week, open-label study conducted in our lipid clinic. After an 8 week dietary baseline phase, we implemented a treatment phase, during which patients received 200 mg/day micronized fenofibrate for 3 months. Serum lipid profiles, including levels of lipoprotein(a) and fibrinogen, serum uric acid, as well as muscle and liver enzymes, were measured after the dietary phase, and at the end of the 3 month treatment period. Compliance was assessed using a clinical interview. A significant decrease in serum uric acid levels was observed in the compliant, while no significant change was noticed in the non-compliant. The compliant had lower serum uric acid concentrations compared to the non-compliant (P < 0.01) after fenofibrate treatment. Furthermore, they demonstrated lower levels of triglycerides and higher high-density lipoprotein (HDL) cholesterol concentrations (P < 0.05 for both parameters). The results show that serum uric acid concentrations may be used as a useful tool to assess compliance with fenofibrate therapy.     

肝硬化和肝癌患者纤溶酶α2-抗纤溶酶复合物的检测

目的探讨肝病患者中血浆纤溶酶α2-抗纤溶酶复合物（PAP）水平及临床意义。方法采用酶联免疫吸附试验法检测71例肝病患者的血浆PAP水平,其中肝硬化25例,肝癌46例,健康对照组10例。结果肝硬化患者的血浆PAP水平为（549．60±331．67）μg／mL,肝癌患者的PAP水平为（390．56±204．12）μg／mL,都显著高于健康对照组的PAP水平（107．89±24．39）μg／mL,差异有统计学意义（P〈0．01）;肝硬化组又明显高于肝癌组,差异也有统计学意义（P〈0．05）;肝硬化分组中的酒精性肝硬化、乙型肝炎后肝硬化和其他原因的肝硬化组的PAP水平分别为（771．66±286．06）、（466．74±371．17）、（512．30±306．03）μg／mL,差异均无统计学意义（P〉0．05）;肝癌组中的初发组PAP值为（378．48±152．45）μg／mL,复发组PAP值为（388．28±273．04）μg／mL,差异也无统计学意义（P〉0．05）。观察血浆PAP与凝血指标的关系,发现PAP与凝血酶原的时间有相关性（r=0．358,P〈0．01）。结论肝病患者中血浆PAP水平的明显升高,提示患者有纤溶亢进和出血倾向,它的检测有助于临床对肝硬化、肝癌病程的观察和治疗。     

Serum creatine kinase isoenzyme BB is a poor index to the size of various brain lesions

We divided patients with brain lesions into three groups: (a) patients with primary or metastatic brain cancer, (b) brain infarctions, and (c) brain contusion(s). We analyzed each patient's sera for creatine kinase isoenzyme BB (CK-BB), using a monoclonal antibody kit (Impres-BB; International Immunoassay Laboratories). Computerized axial tomography (CAT) scans were performed on each patient. The size of the various lesions was measured from the CAT scan and recorded in milliliters. Total CK, CK-BB, and their ratios were compared with the volume of damaged brain tissue. We found no correlation between any of the variables and the various brain lesions. We attribute this lack of correlation to an intact blood-brain barrier, the rapid elimination or inactivation of CK-BB, or some combination of these factors.     

3项指标联合检测肝硬化的临床价值

目的：探讨血清甲胎蛋白（AFP）、丙酮酸激酶M2（M2‐PK）、清蛋白（ALB）联合检测在肝硬化诊断中的临床价值。方法将2011年3月至2013年3月成都市妇女儿童中心医院收治的178例各类肝病患者按照疾病类型分为急性肝炎组52例、慢性肝炎组57例、肝硬化组69例,另选择同期健康体检的健康者60例作为对照组,对比各组研究对象的血清AFP、M2‐PK、ALB水平。结果与对照组相比,急性肝炎组、慢性肝炎组、肝硬化组血清AFP、M2‐PK水平均明显升高,ALB明显降低,组间相比差异有统计学意义（P＜0．05）;与其他3组相比,肝硬化组血清AFP、M2‐PK水平最高,ALB水平最低（P＜0．05）。AFP、M2‐PK水平随着Child‐Pugh分级增高而升高,ALB水平随着Child‐Pugh分级增高而降低,组间相比差异有统计学意义（P＜0．05）。AFP、M2‐PK、ALB联合检测能够提高检测特异性和准确性。结论AFP、M2‐PK、ALB联合检测在肝硬化诊断中有较高的临床应用价值。     

Serum S100beta protein in children with acute recurrent headache: a potentially useful marker for migraine

Objective.—To examine the role of glia-derived S100β protein and to evaluate its use as a biochemical marker in childhood acute recurrent headache.
Methods.—Twenty-five patients with acute recurrent headache (according to International Headache Society criteria) from our department's Headache Clinic were studied. Blood samples for measurement of serum S100β were drawn: (1) ≤3-hour post pain attack from our patients and (2) from 23 healthy controls.
Results.—Of the 25 patients evaluated, 15 suffered from migraine and 10 from tension-type headache (TTH). Statistical analysis of the mean values of S100β levels demonstrated a significant elevation in children with migraine headache, with values higher than those of both children with TTH and controls ( P = .001).
Conclusions.—Our data suggest a direct relation between childhood migraine attacks and increased production of glial S100β protein. Serum S100β determination may be a useful biochemical marker for migraine in acute recurrent headache in childhood.     

肺癌患者肿瘤型M2丙酮酸激酶表达水平及临床意义

目的探讨肺癌患者治疗前后血浆中肿瘤型M2丙酮酸激酶（TuM2-PK）的表达水平及其与肿瘤的近期疗效关系。方法用ELISA法分别检测肺癌患者组（n=80）、良性肺部疾病组（n=60）、健康体检组（n=100）血浆中的TuM2-PK的表达水平，并进行比较分析。结果肺癌组TuM2-PK浓度、阳性率（23．1kU／L、69％）明显高于良性肺疾病组（11．5kU／L、5％）和健康对照组（9．1kU／L、3％）（P〈0．01）。经内科治疗后，病情缓解者血浆TuM2-PK浓度（13．1kU／L）与治疗前（21．6kU／L）相比显著下降（P〈0．05）；病情进展者血浆TuM2-PK浓度（63．5ku／L）与治疗前（23．4kU／L）相比显著升高（P〈0．05）；病情稳定者血浆TuM2-PK浓度治疗前后差异无统计学意义（P〉0．05）。手术后TuM2-PK阳性率显著下降，而化疗后阳性率下降不明显。结论TuM2-PK对肺癌的辅助诊断有较高的临床价值，有助于疗效的评估。     

Serum mitochondrial aspartate aminotransferase activity: not useful as a marker of excessive alcohol consumption in an unselected population

Using an immunochemical method, we measured the activity of the mitochondrial isoenzyme (mAST) of aspartate amino-transferase (EC 2.6.1.1, AST) in the serum of 687 subjects attending the Centre for Preventive Medicine for a health examination. The distributions of the activities were asymmetrical, with mean values of 1.8 U/L (SD 2.0) for men and 1.4 U/L (SD 1.6) for women. The average ratio of mitochondrial to total AST activity was 0.051 (range 0-0.42). In this unselected population we found no change in the mitochondrial activity or in the mitochondrial-to-total ratio attributable to alcohol consumption, even in subjects who consumed more than 88 g per day. Of 35 men with an alcohol consumption greater than 88 g/d, 19 had a serum gamma-glutamyltransferase activity of greater than or equal to 60 U/L, 17 had glutamate dehydrogenase values greater than or equal to 5 U/L, and only nine had an mAST activity greater than or equal to 3 U/L (values corresponding to the 80th percentiles of the total population). We conclude that the test is not particularly useful as a screening procedure in an unselected population under present-day conditions of measurement.     

肿瘤型M2丙酮酸激酶在肺癌诊断中的应用

目的 探讨肿瘤型M2丙酮酸激酶（TuM2-PK）在肺癌患者血中的表达水平及其临床应用价值。方法 用ELISA法分别检测92例肺癌、77例良性肺部疾病患者和106名健康体检者血浆中的TuM2-PK表达水平，并进行比较分析。结果 TuM2-PK浓度和阳性率分别为肺癌组22．1U／ml和71％，良性肺疾病组10．5U／ml和4％，健康对照组8U／ml和3％，肺癌组明显高于后两组（P〈0．01）。Ⅲ、Ⅳ期肺癌患者TuM2-PK阳性率（分别为83％和88％）高于Ⅰ或Ⅱ期肺癌（分别为43％和56％，P均〈0．05）；有淋巴结转移患者TuN2-PK阳性率（79％）高于未发现转移患者（35％，P〈0．05）。TuM2-PK诊断肺癌的敏感度为71．0％，特异度为96．7％，阳性预测值91．5％，阴性预测值86．8％，准确性88．0％。结论 TuM2-PK对肺癌的辅助诊断有较高的临床价值，并对临床分期、判断淋巴结转移及临床治疗具有一定的指导意义。     

Macro creatine kinase type 2: A marker of myocardial damage in infants?

The case histories of two children (aged two months) affected by myocarditis showing an atypical band of serum creatine kinase (EC 2.7.3.2; CK) in the CK isoenzyme electrophoretic pattern are reported. The electrophoretic mobility on cellulose acetate of the atypical iso-CK band and its greater relative molecular mass, the lack of binding with immunoglobulins and the result of CK-BB determination by RIA, allowed us to identify the band with an oligomeric form of the mitochondrial isoenzyme. One child died 2 days after admission, while in the other it was possible to demonstrate reduction and disappearance of the atypical band in concomitance with a marked clinical improvement. Our findings suggest that the oligomeric form of mitochondrial-CK is released in conditions of serious heart muscle damage, and that it may be an indicator of myocardial cellular necrosis in pediatric patients.     

一种血浆肿瘤型M2-丙酮酸激酶检测方法——酶联免疫吸附试验的临床评价

目的评价一种血浆肿瘤型M2-丙酮酸激酶（TUM2-PK）检测方法——酶联免疫吸附试验（ELISA）的测定性能和临床适用性。方法对新的自主开发的TU M2-PK检测方法进行偏倚测定、不精密度测定、回收试验以及对371例临床标本进行测定。结果TU M2-PK检测方法有良好的准确度（偏倚〈5 U/mL）和精密度[变异系数（CV）〈10%]。371份临床标本的TUM2-PK水平测定结果与＂金标准＂病理结果对照,TUM2-PK的敏感性和特异性分别为69.7%（106/152）和86.8%（190/219）,准确度达到80.0%（296/371）。此外,该试剂盒所测得的恶性肿瘤患者的TU M2-PK水平明显高于良性肿瘤患者及正常对照者,差异具有统计学意义（P均〈0.01）。结论TU M2-PK ELISA测定性能良好,具有较好的临床应用价值。     

创伤后肠道通透性改变血浆标志物D乳酸的实验研究

目的：观察创伤时循环Ｄ－乳酸与内毒素的变化及其意义。方法：采用３种大鼠模型：（１）肠系膜上动脉夹闭１小时肠缺血－再灌注损伤模型;（２）３０％总体表面积深Ⅱ～Ⅲ度烫伤模型;（３）总胰胆管人工胆汁逆行灌注的急性坏死性胰腺炎（ＡＮＰ）模型。于各时间点采集颈动脉及门静脉血,分别检测Ｄ－乳酸及内毒素含量。结果：（１）肠缺血１～１．５小时血浆Ｄ－乳酸和内毒素均升高,再灌注后显著升高,二者存在明显正相关（ｒ＝０     

腺苷蛋氨酸辅助蓝光照射对黄疸新生儿肌酸激酶同工酶、转铁蛋白水平及肝功能的影响

目的 研究腺苷蛋氨酸辅助蓝光照射对黄疸新生儿肌酸激酶同工酶(CK-MB)、转铁蛋白(TRF)水平及肝功能的影响.方法 将我院收治的280例黄疸新生儿随机分为对照组(140例,蓝光照射)和观察组(140例,蓝光照射+腺苷蛋氨酸).比较两组的神经肽Y(NPY)、胶质纤维酸性蛋白(GFAP)、CK-MB、TRF水平及肝功能指...     

血浆M2-PK的检测对胃癌的诊断价值

目的探讨血浆M 2-丙酮酸激酶(pyruvate k inaseM 2,M 2-PK)含量变化与胃癌发生的关系及M 2-PK对胃癌的诊断价值。方法采用EL ISA法检测55例正常人、50例胃癌患者、45例急慢性胃炎以及42例胃良性肿瘤患者血浆M 2-PK和血清癌抗原199(CA 199)含量,进行比较。结果胃癌组血浆M 2-PK和血清CA 199含量及阳性率明显高于对照组、急慢性胃炎组及胃良性肿瘤组(P<0.01);胃癌组血浆M 2-PK和血清CA 199含量比较差异无显著性(P>0.05),阳性率比较差异有显著性(P<0.01);正常对照组、急慢性胃炎组及胃良性肿瘤组,血浆M 2-PK、血清CA 199含量及阳性率组间比较差异均无显著性(P>0.05)。结论M 2-PK是检测胃癌较为敏感的肿瘤标志物。M 2-PK的敏感性高于CA 199,具有广泛的临床应用价值。     

Lymphocyte cytosolic protein 2 is a novel prognostic marker in lung adenocarcinoma

ObjectiveLymphocyte cytosolic protein 2 (LCP2) is often ectopically expressed in various human tumors. However, the clinical significance and role of LCP2 in lung adenocarcinoma (LUAD) remain unclear. This study explored the prognostic significance of LCP2 in LUAD patients.MethodsLCP2 expression in LUAD tissues was analyzed using data from The Cancer Genome Atlas and Genotype-Tissue Expression databases. Western blotting was employed to detect LCP2 expression in LUAD. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses were performed to explore signaling pathways mediated by LCP2 co-regulatory genes. Immunohistochemistry was used to examine levels of LCP2 and programmed death ligand 1 (PD-L1) in 68 LUAD patients. Associations between LCP2 expression and clinicopathological features, prognoses, and PD-L1 levels among the LUAD in-patients were analyzed.ResultsAmong the 68 LUAD in-patients, LCP2 expression was correlated with clinical stage and lymph node metastasis. LUAD patients with high LCP2 expression were associated with increased overall survival. LCP2 expression may be associated with an enrichment of several immune functions. Moreover, our immunohistochemistry results demonstrated that LCP2 expression was positively correlated with PD-L1 expression in LUAD tissues.ConclusionsIn the study, LCP2 was found to be a favorable prognostic biomarker in LUAD patients.