幽门螺杆菌感染与慢性胃病关系的检测分析

目的：了解消化性溃疡和慢性胃炎患者感染的幽门螺杆菌（Hp）cagA／vacA优势基因型及不同基因型Hp感染、混合感染与疾病的关系。方法：选择胃窦、胃体双份活检标本均培养出Hp的42例慢性胃炎（CG）和36例消化性溃疡（PU）患者作为研究对象，采用聚合酶链反应检测156份Hp分离株的cagA基因、vacA基因的信号区（s）和中间区（m）亚型，分析Hp基因型及多株Hp混合感染在CG和PU中的分布。部分优势基因型的扩增产物T—A克隆后进行核苷酸序列测定。结果：cagA基因的检测中，78例病人的胃窦标本中有？5例（96．2％）为cagA阳性，相应的胃体标本中，76例（97．4％）为cagA阳性，有1例（1．3％）患者胃窦、胃体检出cagA状态不一的Hp混合菌株。在胃窦标本的vacA基因分型中，sla／ml、sla／m2、sla／mlb、sla／mib—m．24种vacA基因型在78例患者中所占比例分别为6．4％（5／78）、55．1％（43／78）、26．9％（21／78）和1．3％（1／78），多株混合感染为3．8％（3／78）；而相应的胃体标本中，前述四种vacA基因型所占比例依次为6．4％（5／78）、53．8％（42／78）、25．6％（20／78）和3．8％（3／78），多株混合感染为5．1％（4／78）。c8gA＋sla／m2和cagA＋sla／mlb在胃窦标本中占51．3％（40／78）和26．9％（21／78），而在相应的胃体标本中占52．6％（41／78）和25．6％（20／78）。少量胃窦、胃体标本中vacA基因8区和m区不能分型，未发现8lb、82和mla型。联合胃窦、冒体标本分析，16例（20．5％）患者中检出不同基因型的多株Hp菌株，同一胃内多部位采样比单部位采样者有更高的混合感染检出率。HpcagA基因、vaeA基因型、cagA／vaeA基因组合及不同基因型菌株混合感染在CG和PU中的分布差异均无显著性（P〉0．05）。6株8h型Hp菌株的8区扩增产物与报道的ala型60190株核苷酸序列同源性为93．15—94．86％，4株n正型Hp菌株的m区扩增产物与报道的m2型87～203株核苷酸序列之间同源性为93．63—97．61％。结论：cagA＋sla／m2是本地区慢性胃炎或消化性溃疡中最主要的Hp菌株的优势基因型，其次为cagA＋sla／mlb，部分vacAsla／m2型优势分布菌株其核苷酸序列与国外报道的参考菌株比较有较高的同源性，部分患者同时感染不同基因型的多株Hp，但均与所致疾病类型和严重程度无密切相关。     

幽门螺杆菌细胞毒素相关蛋白A、细胞毒素相关蛋白E、细胞空泡毒素A基因型与上消化道疾病的关系

目的分析幽门螺杆菌(Hp)的细胞毒素相关蛋白A(cagA)、细胞毒素相关蛋白E(cagE)、细胞空泡毒素A(vacA)基因型与上消化道疾病的关系。方法选取112例上消化道疾病患者,对其胃黏膜组织中Hp菌株的cagA、cagE、vacA基因型进行检测和分析。结果所有患者胃黏膜样本中的Hp菌株均为cagA基因和cagE基因阳性表达,阳性率均为100%。患者的vacAs1/m~2表型的阳性率最高,分别为54.1%和60.5%,其次为vacAs1/mlb表型和vacAs1/m~-表型,阳性率分别为13.2%~21.1%。消化性溃疡患者和慢性胃炎患者的Hp基因各表型的阳性率的差异均无统计学意义(P0.05)。结论上消化道疾病患者胃黏膜组织中的Hp菌株呈现cagA、cagE、vacAs1/m~2等基因亚型的优势表达,说明这些毒力因子表型在Hp引发和促进上消化道疾病的过程中具有重要的作用,但与上消化道疾病类型缺乏相关性。     

Hp cagA、vacA s1m2基因与萎缩性胃炎疾病诊断的相关性研究

目的研究幽门螺旋杆菌cagA、vacA s1m2基因与萎缩性胃炎疾病诊断的相关性。方法回顾性选取南京梅山医院2017年2月至2018年2月期间内进行胃镜检查的100例胃炎患者为研究对象,其中浅表性胃炎37例,萎缩性胃炎35例,胃溃疡28例。PCR检测三组患者的胃黏膜组织的幽门螺旋杆菌cagA、vacA s1m2基因表达,分析三组患者的cagA、vacA s1m2基因与萎缩性胃炎疾病诊断的相关性。使用ROC曲线,分别对cagA、vacA s1m2单独检测和联合检测,分析其诊断灵敏度之间的差异。结果通过对患者的cagA、vacA s1m2基因分析,三组患者的cagA、vacA s1m2基因阳性情况表达之间的差异存在统计学意义(P 0. 05),其中,cagA基因表达率从高到低依次为胃溃疡、萎缩性胃炎以及浅表性胃炎,而vacA s1m2基因表达率从高到低依次为萎缩性胃炎、浅表性胃炎、胃溃疡。浅表性胃炎、萎缩性胃炎以及胃溃疡患者的诊断与幽门螺旋杆菌的cagA、vacA s1m2的基因相对表达量呈现正相关,联合检测诊断效能显著高于单独检测,差异存在统计学意义(P 0. 05)。结论幽门螺旋杆菌的诊断与患者的cagA基因表达以及vacA s1m2基因表达呈现显著相关性,建议在临床诊断中进行推广。     

不同消化性疾病患者幽门螺杆菌毒力基因类型及意义研究

目的分析用不同消化性疾病来源的幽门螺杆菌(Hp)毒力基因的检测结果及其意义。方法收集该院胃镜室于2015年1月至2016年7月采集的628例患者胃活检标本,分离培养Hp,检测其携带的毒力基因,并探讨Hp与慢性萎缩性胃炎(CAG)、慢性浅表性胃炎(CSG)、消化性溃疡(PUD)的相关性。结果 628份胃活检标本中成功分离出214株Hp,选取其中172株提取DNA,发现Hp携带cagA、vacA、dupA、iceA、oipA、luxS多种毒力基因,其中vacA s1m1是CSG的危险因素,vacA s1m2与iceA1+/iceA2+提高PUD的发生率,dupA+提高十二指肠溃疡危险度。结论不同消化性疾病与Hp感染密切相关,dupA可考虑作为Hp致十二指肠溃疡的标记基因,vacA s1m2与iceA1+/iceA2+增加了发生PUD的危险性。     

上海崇明地区幽门螺杆菌cagA基因多态性与感染临床结局的关系

目的探讨上海崇明地区幽门螺杆菌（Helicobacter pylori,Hp）的cagA基因的多态性与感染临床结局的关系。方法采用PCR法检测100株临床分离株cagA基因亚型,获得东亚型和西方型2种基因型。分析cagA基因亚型与消化道疾病间的关系。结果100株Hp中72株检出cagA基因,其中81．9％为cagA基因东亚型,15．3％为西方型,2．8％为同时存在着2种基因型。胃癌与慢性萎缩性胃炎患者中cagA基因东亚型菌株均显著高于胃溃疡、十二指肠溃疡患者,而十二指肠溃疡cagA基因西方型菌株的比率显著高于其余4种疾病类型。结论本地区感染Hp以cagA基因东亚型为主,cagA基因东亚型菌株与胃癌、慢性萎缩性胃炎密切相关,而cagA基因西方型菌株与十二指肠溃疡密切相关。推测cagA基因多态性对由Hp引起的感染性疾病的发病机制可能起一定的作用。     

幽门螺杆菌cagA基因和血清CagA抗体与VacA表达关系的研究

目的：探讨幽门螺杆菌cagA基因和血清CagA抗体与VacA表达的关系。方法：用聚合酶镇反应法（PCR）测定由62例慢性胃炎、消化性溃疡和胃癌患者胃窦粘膜分离获得Hp菌株;用酶免疫技术测定患者血清HpCagA抗体;用Hela细胞培养法测定Hp菌株VacA活性。结果：cagA基因阳性和阴性Hp菌株表达VacA阳性率分别为40．00％和33．33％（P＞0.05）;血清CagA抗体阳性和阴性患者感染Hp菌株体外产生VacA阳性率分别为33．33％和42．11％（P＞0.05）。结论：HpcagA基因和血清CagA抗体与VacA表达之间无相互依赖关系,vacA基因是一个独立的标志物。     

十二指肠球部变异与反流性胃炎关系初探

目的 分析十二指肠球部变异患者胃粘膜病变、幽门螺杆菌（HP）感染及胆汁反流变化的关系。方法对165例十二指肠球部变异患者行胃镜及胃粘膜活检,分析其胃粘膜炎症反应、HP感染、胆汁反流的关系。结果本组HP感染率21．8％（36／165）,而同期慢性胃炎HP感染率为41．8％（2750／6579）。差异有统计学意义（P〈0．01）。表明HP感染与胃内胆汁反流缺乏必然联系。Ⅲ度反流组肠化生和萎缩检出率显著高于Ⅰ度反流组,分别为39．6％（21／53）、14．3％（5／35）及43．4％（23／53）、17．1％（6／35）,（P〈0．01）。十二指肠球部变异及幽门口开放越显著,胃窦部粘膜炎症程度越严重。结论十二指肠球部变异及幽门括约肌弛缓导致的胃窦粘膜损伤主要因素为胆汁反流。     

小儿幽门螺杆菌cagA基因变异性探讨

本研究应用 3对不同引物 ,通过聚合酶链反应 (PCR)技术扩增具有细胞毒素相关基因 (cagA基因 )DNA ,检测浙江地区儿童感染幽门螺杆菌 (Hp)cagA基因阳性 (cagA+ )菌株阳性率 ,探讨cagA基因的变异性及其CagA蛋白表达情况。一、材料与方法1 研究对象 :2 0 0 0年 12月～ 2 0 0 1年 5月间经胃镜检查、组织活检证实为Hp感染患儿 5 0例 ,男 31例 ,女 19例 ,平均年龄 9岁 (范围 4～ 15岁 )。2 胃粘膜标本 :取距幽门孔 3cm内胃窦粘膜 3～ 4块 ,分别进行病理切片 ;快速尿素酶 (福建三强生物制品公司试剂 )试验 (RUT) ;荧光定量PCR检测HpUreA…     

Hp菌株cagA、picB基因与胃粘膜IL-8表达、消化性溃疡关系的初步研究

目的 :初步探讨Hp菌株cagA、picB基因表达与胃粘膜IL - 8分泌 ,消化性溃疡发病的关系。方法 :采用分离培养技术行Hp培养 ;PCR扩增技术检测cagA、picB基因表达 ,ELISA法检测胃窦部IL - 8表达水平。结果 :消化性溃疡患者胃窦部粘膜IL - 8表达水平显著高于慢性胃炎患者 (t=15 .5 2 ,P <0 .0 1) ;消化性溃疡患者中cagA / picB 菌株感染率显著高于慢性胃炎患者 (χ2 =9.99,P <0 .0 1) ;cagA / picB 菌株感染者胃窦粘膜IL - 8表达水平显著高于cagA-/ picB-菌株感染者 (t=5 .43,P <0 .0 1)。结论 :IL - 8在Hp相关性消化性溃疡中起重要作用 ;cagA / picB 菌株感染可能与消化性溃疡的发病有关     

上海市崇明地区幽门螺杆菌耐药性分析及cagA基因检测

目的探讨上海市崇明地区含细胞毒素相关基因A（cagA）的幽门螺杆菌（Hp）的感染情况及cagA基因与Hp的耐药性关系。方法对150株临床分离菌株，采用PCR法检测Hp的cagA基因，同时采用纸片扩散法作药敏试验。结果崇明地区Hp对甲硝唑、替硝唑、阿莫西林、克拉霉素、阿奇霉素和呋喃唑酮的耐药率分别为32%、10、2、0、0和0。78．7％的Hp菌株含有cagA基因。结论本地区阿莫西林、阿奇霉素、呋喃唑酮可作为根除Hp的首选药物。本地区Hp感染以Ⅰ型菌株为主，含cagA基因Hp菌株对甲硝唑耐药率明显低于不含cagA基因Hp菌株。     

幽门螺杆菌cagA、vacA抗体与胃十二指肠疾病的相关性研究

目的：探讨幽门螺杆菌（Hp)毒力基因cagA,vacA抗体与胃十二指肠疾病之间的关系。方法：采用免疫印迹法检测440例胃十二指肠疾病患者血清中的cagA,vacA抗体。结果：cagA,vacA抗体在440例患者中的检出率分别为73%，37.0%。在慢性胃炎（CG），十二指肠肠球部溃疡（DU），胃癌（GC）患者专利号，cagA,vacA抗体摄影性率分别为62.9%,76.1%,96.9%与33.0%,31.0%,62.5%；经u检验显示；慢性胃炎组与十二指肠球部溃疡组比较，无明显差异。胃癌组与慢性胃炎组，十二指肠球部溃疡组比较，有显著性差异。结论：本文通过患者血清中Hp抗体（cagA和vacA)的检测。推知其cagA和vacA抗体的表达状况，可为胃十二指肠疾病的诊断提供血清中Hp抗体（cagA和vacA)的检测，推知其cagA和vacA抗体的表达状况，可为胃十二指肠疾病的诊断提供依据。但不能作为区分Hp感染所致胃十二指肠疾病的单一指标。     

Analysis of the vacA,cagA, cagE,iceA, and babA2 genes in Helicobacter pylori from sixty-one pediatric patients from the Midwestern United States

This study was designed to characterize H. pylori from pediatric gastric biopsy specimens in terms of several genes (vacA, cagA, cagE, iceA1, iceA2, and babA2) proposed to be involved in the pathogenesis of this organism. Many of these genes have been studied in adult H. pylori isolates, however, these genes have not been well characterized in H. pylori from children. Using PCR we observed that 44% of the H. pylori in our biopsies shared two common genotypes (vacA s1b m1, cagA, cagE, iceA2 +/- babA2). While 26% of the H. pylori had unique genotypes. The cag pathogenicity island associated genes, cagA and cagE, were found together in 64% or our H. pylori, while 84% were iceA2 positive. The presence of the babA2 gene has been proposed to be associated with a higher risk of H. pylori related diseases, however, we found that only 36% of our H. pylori contained this gene.     

小儿幽门螺杆菌感染与慢性胃炎

目的 :研究幽门螺杆菌 (Helicobacterpylori,Hp)感染与小儿不同类型慢性胃炎之间的关系。 方法 :对我院 2 0 0 0年—2 0 0 2年 14 0 4例具有上消化道症状 ,经胃镜检查确诊为慢性胃炎的患儿进行胃黏膜活检 ,分别行病理组织学检查及快速尿素酶试验、改良Giemsa染色找Hp ,同时患儿行血清抗HpIgG检测和 (或 ) 13 碳 尿素呼气试验 (13 C UBT)和 (或 )粪幽门螺杆菌抗原检测 (Helicobacterpyloristoolantigen ,HpSA)。 结果 :14 0 4例慢性胃炎患儿中 ,Hp感染率为 4 5 .3%。各种不同类型慢性胃炎中 ,以胃镜下结节性胃炎和消化性溃疡伴慢性胃炎的患儿Hp感染率为最高 ,分别为 71.2 %和 6 8.0 % ,明显高于其他胃炎组 (P <0 .0 1)。胃黏膜病理组织学改变 ,Hp感染组引起的黏膜炎症程度较重 ,淋巴滤泡形成比例明显较对照组高 (P <0 .0 1)。结论 :小儿时期Hp感染率已较高 ,随年龄增长Hp感染率增高。并且与小儿胃十二指肠疾病关系密切 ,其中与胃镜下结节性胃炎和消化性溃疡伴慢性胃炎最为密切。胃黏膜组织炎症程度越重 ,Hp感染阳性率越高。     

cagA vacA alelles and babA2 genotypes of Helicobacter pylori associated with gastric disease in Brazilian adult patients

Helicobacter pylori is an important human pathogen that causes chronic gastritis and is associated with development of peptic ulcer disease and gastric malignancies. The vacuolating cytotoxin (vacA), cagA gene, and babA2 gene are important virulence factor involving gastric diseases. Eighty-nine Helicobacter pylori-positive gastric biopsies were analyzed by polymerase chain reaction and Southern blotting for H. pylori detection and genotyping with primer pairs from each virulence gene. Fifty-three strains (59%) were common vacA genotype s1/m1, and only 14 (16%) were s2/m2, 12% of strains was found to have multiple infection. The cagA presence was detected in 48% (43 strains) and babA2 gene was detected in 44% of our H. pylori strains. We observed high percentage of s1/m1 strains with chronic gastritis and peptic ulcer and a significant correlation between cagA presence with the s1 allele and babA2 gene with chronic gastritis.     

广州地区消化道疾病患者中H.pylori ureA和vacA s1基因及cagA基因亚型分布

目的 分析研究广州地区消化道疾病患者中H.pylori ureA、vacA s1基因和cagA基因亚型(ABC、ABD、ABAB、AAD等)的分布状况及其与胃黏膜病理检测结果间的相关性.方法 随机选取227例消化道疾病患者的胃黏膜标本,分别来自病理组织学检测无病理改变者46例,慢性胃炎130例,消化性溃疡29例,萎缩性胃炎15例,胃癌7例.并用实时荧光定量PCR检测H.pylori ureA基因、vacA s1基因,用PCR扩增cagA羧基端EPIYA基序所在区,然后测序确定其亚型.以保守基因ureA的存在判断H.pylori感染.结果 227例消化道疾病患者中,有50.7% (115/227)的患者H.pylori阳性,其中,vacA s1基因阳性91.3%(105/115),cagA基因阳性78.3%(90/115).4种cagA-EPIYA亚型分布为,ABC 17.8%(16/90)、ABD 78.9%(71/90)、AAD 2.2%(2/90)、ABAB 1.1%(1/90).无病理改变组中H.pylori 阳性32.6%(15/46),vacA s1基因阳性28.3%(13/46),cagA基因阳性26.1%(12/46);慢性胃炎组H.pylori 阳性48.5%(63/130),vacA s1基因阳性43.8%(57/130),cagA基因阳性36.2%(47/130);溃疡组H.pylori 阳性72.4%(21/29),vacA s1基因阳性65.5%(19/29),cagA基因阳性55.2%(16/29);萎缩性胃炎组H.pylori 阳性66.7%(10/15),vacA s1基因阳性66.7%(10/15),cagA基因阳性66.7%(10/15);胃癌组H.pylori阳性85.7%(6/7),vacA s1基因阳性85.7%(6/7),cagA基因阳性71.4%(5/7).H.pylori在不同胃黏膜病理组的分布差异有统计学意义(χ2=16.72;P<0.01),溃疡、萎缩性胃炎、胃癌组中H.pylori的分布明显高于无病理改变与炎症组(χ2=16.02;P<0.01).但在H.pylori阳性患者中,强毒力因子vacA s1基因(χ2=2.00;P=0.74)、cagA基因(χ2=3.44;P=0.49)及cagA-EPIYA亚型(χ2=3.66;P=0.45)在无病理改变、炎症、溃疡、萎缩性胃炎及胃癌组中的分布差异均无统计学意义.结论 广州消化道疾病患者中H.pylori的感染与胃黏膜病理改变显著相关,而广州地区消化道疾病患者中H.pylori高毒力亚型的强致病性并不明显,需扩大标本量,再细化疾病种类进一步分析高毒力H.pylori对胃肠道疾病发生的影响.

Abstract：

Objective To detect the distribution of H.pylori ureA, vacA s1 gene and cagA subtype(ABC, ABD, ABAB, AAD, et al) in patients with digestive diseases in Guangzhou and investigate the relationship with the pathological findings of gastric mucosa.Methods A total of 227 randomly selected gastric mucosa from patients with digestive diseases were enrolled in the research, including 46 without pathological changes, 130 with chronic gastritis, 29 with peptic ulcer, 15 with atrophic gastritis and 7 with gastric cancer.Real-time PCR assay were used to detect Helicobacter pylori ureA gene and vacA s1 gene.EPIYA motifs in the 3′ region of cagA were amplified by conventional PCR followed by subtype sequencing. The conserved gene ureA was used to detect H.pylori infection.Results Among the 227 patients with digestive diseases, 50.7% (115/227) patients were H.pylori positive, in which 91.3%(105/115) carried vacA s1 and 78.3% (90/115) carried cagA. Four types of cagA-EPIYA subtype were detected, including ABC 17.8%(16/90), ABD 78.9%(71/90), AAD 2.2%(2/90) and ABAB 1.1%(1/90).In the non-pathological change group, 32.6% (15/46) were H.pylori positive, in which 28.3% (13/46) carried vacA s1 and 26.1% (12/46) carried cagA;in chronic gastritis group, it was 48.5% (63/130), 43.8% (57/130) and 36.2% (47/130), respectively;in ulcer group, it was 72.4% (21/29), 65.5% (19/29) and 55.2% (16/29), respectively;in atrophic gastritis group, it was 66.7% (10/15), 66.7% (10/15) and 66.7% (10/15), respectively;in gastric cancer group, it was 85.7% (6/7), 85.7% (6/7) and 71.4% (5/7), respectively.The distribution of H.pylori among the 4 groups had statistical significance (χ2=16.72;P<0.01). H.pylori was more prevalent in ulcer, atrophic gastritis and cancer group than in inflammation group and non-pathological change group (χ2=16.02;P<0.01).In patients infected by H.pylori, there was no significant difference in the distribution of vacA s1 gene as high virulence factors among non-pathological change, inflammation, ulcer, atrophic gastritis and cancer group (χ2=2.00;P=0.74), as well as cagA (χ2=3.44;P=0.49) and EPIYA subtypes (χ2=3.66;P=0.45).Conclusions H.pylori infection is significantly associated with the pathological change of gastric mucosa for patients with digestive diseases in Guangzhou, while the relationship with the pathogenicity of H.pylori with high virulence genotype is not significant.More samples and diseases reclassification are needed to make an advanced analysis of the effect of H.pylori with high virulence in gastrointestinal diseases development.     

Genotyping of Helicobacter pylori strains from gastric biopsies by multiplex polymerase chain reaction. How advantageous is it?

Recent application of multiplex polymerase chain reaction (PCR) for genotyping Helicobacter pylori direct from biopsies revealed variable results (detection of amplicons from DNA extracted by boiling biopsies, variable size amplicons and deletions, uniform intensity of amplicon bands). We aimed to look at how applicable the technique is for determining cagA and vacA genotypes and to correlate the results with the severity of the disease. H. pylori strains from 52 patients (35 duodenal ulcers [DUs], 7 gastric ulcers [GUs], 10 gastritis) were included. Three antral biopsies were obtained for Campylobacter-like organism (CLO) and PCR. Primers for cagA, vacA s1s2, and m1m2 alleles were used. No PCR amplicons were detected from boiling biopsies; thus, DNA was extracted by QIAamp kit. H. pylori was positive in 84.6% of the patients (85.7% DU, 100% GU, and 70% gastritis). The cagA gene was detected in 86.6% DU, 71.4% GU, and 57.0% gastritis patients. The vacA allelic distribution among cagA-positive strains was 80.7% s1m1 in DU and 60.0% in GU patients, whereas 75.0% of gastritis had s1m2. No variability in the amplicon sizes was found, and the intensity of the amplicon bands was not uniform. A deleted band of approximately 420 bp below the m1 band was detected in strains from 2 DU and 1 GU patients. Although the multiplex PCR is a rapid and an effective tool for detecting several genes in a single-step system, one has to adjust for optimization of the technique when genotyping H. pylori direct from biopsies. A significant association was found between the cagA-positive vacA-s1m1 genotype and peptic ulcers.     

儿童幽门螺杆菌感染与缺铁性贫血的相关性

目的探讨儿童中幽门螺杆菌（Hp）感染与缺铁性贫血（IDA）的相关性。方法以因慢性腹痛而行胃镜检查及Hp检测的60例患儿为观察对象,根据检查结果分为Hp阳性慢性胃炎组、Hp阴性慢性胃炎组及Hp阳性胃部镜下无损害组三组。所有病例均检测血红蛋白（Hb）、平均红细胞体积（MCV）、血清铁（SI）、血清铁蛋白（SF）、总铁结合力（TIBC）等IDA指标。比较三组之间的IDA指标和IDA伴发率。结果三组病例IDA指标两两比较显示,Hp阴性慢性胃炎组分别和Hp阳性慢性胃炎组、Hp阳性胃部镜下无损害组的IDA指标比较差异均有统计学意义（P〈0.01,P〈0.05）,而Hp阳性慢性胃炎组与Hp阳性胃部镜下无损害组之间的比较差异则无统计学意义（P〉0.05）。Hp阴性慢性胃炎的IDA伴发率均明显低于Hp阳性慢性胃炎组和阳性胃部镜下无损害组（P〈0.05）,Hp阳性慢性胃炎组与Hp阳性胃部镜下无损害组之间的IDA伴发率差异无统计学意义（P〉0.05）。结论儿童幽门螺杆菌感染与缺铁性盆血两者之间具有显著的相关性,其相关性较慢性胃炎更为密切。