The Role of Nitric Oxide in Hyperoxic Lung Injury in Premature Rats

The mechanism of hyperoxic lung injury has notbeen fully elucidated. Recentstudies showed thatni-tric oxide( NO) synthesized by nitric oxide synthase( NOS) seems to play an important role in oxygentoxicity,but no conclusive agreement has beenreached.Furthermore,no reports were available onthe preterm animal models close to clinical status.Inthis study,pre- term rats were exposed to over 90 %oxygen and the role of endogenous NO in neonatalhyperoxic lung injury was explored by studying theeff…     

Expression of Apoptosis and Inducible Nitric Oxide Synthase in Trophoblastic Cells in Early Spontaneous Abortion

The pregnant immune,especially the function of placenta barrier has been thefocus of recent study in this field.Placenta,as an immune organ,produces a lageamountof activated molecule and isregulated by neural- immune- endocrinenetworks.Butwhat is more importantis thatplacenta actas a barrier to protectthe fetus frommaternal infection or immune challenge.Syncytiotrophoblast has been confirmed tobe the key to the barrier' s function.With its dysfunction,the possibility ofpenetration of the barri…     

Experimental Study on the Effects of Aprotinin on Myocardial Ischemia and Reperfusion

Aprotininhasbeenwidelyusedincardiopulmonarybypasspr0cedurebecauseofitsadvantagesofreducingbleedingandbloodtransfusionduringandafteropenheartsurgery[".However,despitethewideuseofaprotinin,theeffects0fthedrugonmyocardialperformanceofnormallyper-fusedheartsandischemicmyocardlumre-mainunkn0wn.Thepresentstudywasde-signedt0evaluatetheeffectsofaprotinin0nn0n-ischemicandlschemicmyocardlalmetab0lism,cardiacfunctionandstructureintheisolatedperfusedw0rkingratheartpreparation.1MATERIALSANDMETH0DS1.…     

A quantitative study on vascular angiotensin II receptors in rats with portal hypertension

Summary Angiotension-I (A-I) receptor maximal binding capacity (B_max) and dissociation constants (K_d) of different blood vessels in rats with prehepatic portal hypertension were studied by radioligand binding analysis. The results showed that the A-I receptor B_max in the thoracic aorta, superior mesenteric artery and portal vein of portal hypertensive animals (113. 7±19. 4 fmol/mg protein, 206. 9 ±39. 3 fmol/mg protein and 31. 5±9. 2 fmol/mg protein respectively) was all significantly lower than that of controls (146. 8±24. 5 fmol/mg protein, 297. 2±44. 7 fmol/mg protein and 53. 4±12. 1 fmol/mg protein respectively,P<0. 01). The A-I receptor K_d in the superior mesenteric artery was markedly increased in portal hypertensive animals (1. 03±0. 11 nmol/L) compared with that in controls (0. 88±0. 08 nmol/L,P<0. 05). In the thoracic aorta and portal vein, the A-I receptor K_d in portal hypertensive animals was slightly higher than that in controls, but no significant difference was observed between the two groups. The results suggested that the vascular hyporesponsiveness to A-I in portal hypertension was caused partially by a reduction in number and a decrease in affinity of vascular A-I receptors, and these changes might possibly lead to the formation of hyperdynamic circulation.     

MMP-25在早孕绒毛和蜕膜中的表达及意义的研究

目的研究基质金属蛋白酶-25 (matrix metalloproteinase-25，MMP-25)在正常早期妊娠和自然流产患者绒毛及蜕膜组织中的表达特点，探讨其在胚胎植入中的作用及其与自然流产的关系。方法采用半定量RT-PCR和免疫组化法检测60例正常早期妊娠和40例自然流产患者绒毛及蜕膜组织中MMP-25mRNA及蛋白的表达。结果① MMP-25mRNA在正常早期妊娠的绒毛及蜕膜组织中均有表达，且绒毛组织中MMP-25mRNA表达水平随妊娠周数的增加逐渐上升，各孕周组间相比差异有统计学意义(P＜0.05)。自然流产组绒毛及蜕膜组织中MMP-25mRNA的表达明显低于正常妊娠组(P＜0.05)。② 正常妊娠组的绒毛组织中，MMP-25主要定位于细胞滋养细胞和合体滋养细胞，并且随妊娠周数增加表达逐渐增强，与RT-PCR结果吻合；自然流产组仅在合体滋养细胞中有表达，两组平均光密度分别为0.21±0.02和0.13±0.04，差异有统计学意义(P＜0.05)。蜕膜组织中MMP-25主要在腺上皮细胞表达，自然流产组的表达微弱，两组平均光密度分别为0.25±0.13和0.21±0.12，差异有统计学意义(P＜0.05)。结论MMP-25表达变化与滋养细胞浸润能力相吻合，且蜕膜组织中呈阳性表达，推测其参与胚胎植入及胎盘形成过程中滋养细胞浸润活性调控及子宫内膜组织重构；自然流产患者MMP-25表达水平降低，造成滋养细胞浸润能力下降，可能与自然流产的发生有关。     

Effects of carvedilol on cardiomyocyte apoptosis and gene expression<Emphasis Type="Italic">in vivo</Emphasis> after ischemia-reperfusion in rats

Summary The effects of carvedilol on cardiomyocyte apoptosis and expression of bcl-2, bax genes following ischemia (0.5 h) and reperfusion (48 h)in vivo and the possible biological mechanism of carvedilol inhibiting cardiomyocyte apoptosis were studied. The left anterior descending artery in Wistar rats were ligated to establish ischemia-reperfusion (I/R) models. The model animals were divided into two groups: I/R group, the model rats not subject to other treatments except ischemia-reperfusion (n=8): carvedilol-treated group (n=8), I/R model rats treated with carvedilol. Eight rats in the sham-operated group were subjected to only experimental open operation. The number of apoptotic cardiomyocyte was determined by TUNEL staining. Immunohistochemistry andin situ hybridization histochemistry (ISHH) were used to detect the expression of bcl-2 and bax genes. Image processing system was used to quantitatively dispose the positive metric substances of both immunohistochemistry and ISHH through the average optical density (OD) value. The results showed that the number of the apoptotic cells were 36.18±9 (I/R group), 0–1 (sham-operated group) and 9.5±3 (carvedilol-treated group) in each visual field respectively with the difference being very significant among the groups (P<0.001). The OD values of bcl-2 protein in sham-operated group, I/R group and carvedilol-treated group were 0.14±0.01, 0.08±0.02 and 0.15±0.03, respectively. The OD values of bcl-2 mRNA in sham-operated group, I/R group and carvedilol-treated group were 0.08±0.01, 0.06±0.01 and 0.09±0.01, respectively. There was no significant difference between carvedilol-treated group and I/R group (P>0.05). The OD values of bax protein in I/R group, sham-operated and carvedilol-treated-treated group were 0.13±0.02, 0.07±0.01, 0.06±0.01, respectively. There was very significant difference between carvedilol-treated group and I/R group (P<0.01). Bcl-2/bax ratio was 1.07±0.14 (I/R group), 1.28±0.16 (sham-operated group), 2.5±0.26 (carvedilol-treated group) respectively with the difference being very significant between carvedilol-treated group and I/R group (P<0.05). It was indicated that carvedilol could inhibit cardiomyocyte apoptosis following ischemia and reperfusion, which was related to the increased bcl-2/bax ratio due to inhibition of bax gene expression. ZENG Hesong, male, born in 1965, Associated Professor This project was supported by a grant from the National Natural Sciences Foundation of Hubei Province (No. 2000J050).     

监测反复自然流产者尿CMV-DNA的研究

[目的]探讨反复自然流产与巨细胞病毒感染的关系.[方法]采用PCR方法对212例反复流产(RAS)妇女及2 602例无流产史早孕妇女的尿液进行CMV-DNA检测.[结果]RAS组CMV-DNA阳性率1.89%,对照组CMV-DNA阳性率0.46%,两者有显著性差异(x2=7.05,P<0.05).[结论]RAS与巨细胞病毒感染有一定的相关性.     

Sex Difference in the Repolarization Currents of Rabbit Ventricular Cells

Female gender is associated with an increasedrisk of drug-associated torsade de pointes (TdP) .Many kinds of drugs such as class ⅠAand class Ⅲantiarrhythmia , some antibiotics , antidepressantdrugs have the potential of inducing TdP. It isthought that the slower repolarization of femaleheart accounts for the higher incidence of TdP.However ,the current difference between male andfemale is poorly known. Our previous experi menthas shown that rabbits presented the same differ-ence of TdP a…     

mRNA expression of basic fibroblast growth factor from a single intratracheal instillation of papain-induced emphysema in rats

Basic fibroblast growth factor(b FGF) is apolypeptide growth factor which promotes the growthand proliferation of cells,and plays an importantrolein maintaining the growth and development of thebody and promoting the injury and repair of varioustissues.The purpose of this study was to investigatethe mechanism of pulmonary interstitial fibrosis in thedevelopmentofemphysema by studying the expressionof b FGF m RNA in a single intratrachealinstillation ofpapain- induced ratemphysema.1　 MATE…     

正常早孕及反复流产妇女血清中TNFα、IL-6水平的检测

目的 :探讨肿瘤坏死因子 (TNFα)、白细胞介素 6(IL- 6)与正常早孕、反复流产的关系。方法 :采用 ELISA方法对 30例正常非孕妇女、30例正常早孕妇女及 60例反复流产妇女血清中TNFα、IL - 6水平检测。结果 :正常早孕妇女血清中 TNFα明显低于正常非孕妇女 (P<0 .0 5) ,正常早孕妇女血清中 IL - 6水平与正常非孕妇女无明显差异 (P>0 .0 5) ,反复流产组血清中 TNFα、IL- 6明显高于正常早孕组 (P<0 .0 1 ) ,其中难免流产组明显高于先兆流产组 (P<0 .0 5)。结论 :提示正常早孕妇女 TNFα分泌受抑制 ,血清中 TNFα、IL- 6水平升高与反复流产有关 ,可作为预测流产的指标     

蜕膜组织TNFR1基因表达与原因不明早期自然流产关系探讨

目的检测蜕膜组织肿瘤坏死因子受体1(Tumornecrosisfactorreceptor,TNFR1)的表达含量及其组织学、细胞学定位,探讨不明原因早期自然流产与患者TNFR1基因表达的关系。方法采用流式细胞仪检测技术,检测37例孕6~10周不明原因自然流产患者(流产组)和34例同期妊娠的健康妇女(对照组)蜕膜组织TNFR1的表达含量;并应用免疫组化方法,检测其中31例流产组和30例对照组蜕膜组织TNFR1表达的组织学、细胞学定位。结果(1)流式细胞仪检测流产组、对照组蜕膜组织中TNFR1的表达含量分别为(16.48±7.2)%,(12.52±5.6))%,流产组高于对照组,差异具有显著性(t=2.24,P<0.05)。免疫组化检测结果显示流产组蜕膜腺体上皮、间质、血管内皮细胞TNFR1表达率分别为77.4%,93.5%和67.7%,对照组分别为96.7%,70.0%和23.3%。2组腺体上皮细胞TNFR1表达率无显著性差异(P>0.05);流产组蜕膜间质、血管内皮细胞TNFR1表达率均高于对照组,差异均有显著性(P<0.05,P<0.05)。结论蜕膜组织中TNFR1表达水平升高,主要分布在蜕膜间质和血管内皮细胞表面,这种改变可能与不明原因早期自然流产有关。     

Clinical significance of a myeloperoxidase gene polymorphism and inducible nitric oxide synthase expression in cirrhotic patients with hepatopulmonary syndrome

The clinical significance of a myeloperoxidase (MPO) gene polymorphism and inducible nitric oxide synthase (iNOS) expression in cirrhotic patients with hepatopulmonary syndrome (HPS) was explored. Enrolled subjects were divided into three groups according to their disease/health conditions: the HPS group (cirrhotic patients with HPS; n=63), the non-HPS group (cirrhotic patients without HPS; n=182), and the control group (healthy subjects without liver disease; n=35). The distribution of the MPO-463 G/A geno...     

组织蛋白酶B和L在早期妊娠绒毛及蜕膜中的表达

目的 观察组织蛋白酶B（cathepsin B，CB）和L（cathepsin L，CL）在人早期妊娠绒毛及蜕膜中的表达．探讨它们在胚胎着床及发育过程中的作用。方法 研究组包括：正常早期妊娠行人工流产者30例、早期自然流产者25例、正常分泌期子宫内膜lO例和葡萄胎15例。应用免疫组织化学法检测CB和CL的表达。结果 CB、CL主要阳性表达于正常早期妊娠滋养层细胞和蜕膜细胞：CB在正常分泌期内膜．正常早期妊娠蜕膜、自然流产蜕膜中的阳性率表达分别为10％（1／10）、83.3％（25／30）、32．0％（8／25）；CL则分别为0、63.3％（19／30）、32．0％（8／25）；CB和CL在3组间的表达有显著性差异（P〈0．05）。CB在早期妊娠绒毛、自然流产绒毛、葡萄胎组织中表达强阳性率分别为10．0％（3／30）、0.66．7％（10／15）；CL则分别为26．7％（8／30）、4．0％（1／25）、80．0％（12／15）。CB和CL在3组间的表达有显著性差异（P〈0．05）。结论 CB和CL在正常和异常妊娠蜕膜和绒毛中的表达差异提示它们胚胎着床过程中可能起着很重要的作用。     

子宫动脉栓塞术对产后出血患者并发症及再次妊娠围生期妊娠结局的影响

目的 探讨子宫动脉栓塞术（UAE）对产后出血（PPH）患者并发症及再次妊娠围生期妊娠结局的影响。方法 选择2015年1月至2019年12月唐山市妇幼保健院妇接收的79例UAE治疗的PPH患者作为UAE组,选择同期在我院接受子宫动脉结扎术（UAL）治疗的78例PPH患者作为UAL组。比较两组术中及术后恢复情况、卵巢功能指标水平、术后近远期并发症发生率及再次妊娠围生期妊娠结局。通过logistic回归分析确定产后出血治疗后远期并发症发生的影响因素。结果 UAE组术中失血量[（959.70±203.15）mL]、止血时间[（30.59±5.81）min]、住院时间[（7.33±1.58）d]、产后月经恢复时间[（72.14±16.42）d]均少于UAL组,产后首次月经量/妊娠前平均月经量（1.13±0.35）多于UAL组,差异均有统计学意义（P<0.05）;UAL组术后抗缪勒氏管激素（AMH）、雌二醇（E2）水平较术前降低（P<0.05）,卵泡刺激素（FSH）水平较术前增高（P<0.05）,两组术前后AMH、E2、FSH的差值比较,差异有统计学意义（P<0.05）;U...     

Effects of Phorbol-12,13-dibuterate on Sodium Currents and Potassium Currents in Rat Trigeminal Ganglion Neurons

The effects of phorbol-12,13-dibuterate (PDBu) on total sodium current (INa-total), tet-rodotoxin-resistant sodium current (INa-TTXr), 4-AP-sensitive potassium current (IA) and TEA-sensitive potassium current (IK) in trigeminal ganglion (TG) neurons were investigated. Whole-cell patch clamp techniques were used to record ion currents in cultured TG neurons of rats. Results revealed that 0.5 μmol/L PDBu reduced the amplitude of INa-total by (38.3±4.5)% (n=6, P<0.05), but neither the G-V curve (control: V0.5 =-17.1±4.3 mV, k=7.4±1.3; PDBu: V0.5=-15.9±5.9 mV, k=5.9±1.4; n=6, P>0.05) nor the inactivation rate constant (control: 3.6±0.9 ms; PDBu: 3.6±0.8 ms; n=6, P>0.05) was altered. 0.5 μmol/L PDBu could significantly increase the amplitude of INa-TTXr by (37.2± 3.2)% (n=9, P<0.05) without affecting the G-V curve (control: V0.5=-14.7±6.0 mV, k=6.9± 1.4; PDBu: V0.5=-11.1±5.3 mV, k=8.1±1.5; n=5, P>0.05) or the inactivation rate constant (control: 4.6±0.6 ms; PDBu: 4.2±0.5 ms; n=5, P>0.05). 0.5 μmol/L PDBu inhibited IK by (15.6±5.0) % (n=16, P<0.05), and V0.5 was significantly altered from - 4.7±1.4 mV to -7.9 ±1.8 mV (n=16, P<0.05). IA was not significantly affected by PDBu, 0.5 μmol/L PDBu decreased IA by only (0.3±3.2)% (n=5, P>0.05). It was concluded that PDBu inhibited INa-total but enhanced INa-TTXr, and inhibited IK without affecting IA. These data suggested that the activation of PKC pathway could exert the actions.     

早期自发流产妇女血清抗子宫内膜抗体的研究

采用酶联免疫吸附试验 (EL ISA)对 10 5例早期自发流产妇女血清中抗子宫内膜抗体 (EMAb)进行检测 ,并与正常生育组对照研究。结果表明 ,早期自发流产妇女血清中 EMAb的阳性率显著高于正常生育组 (P<0 .0 1)。流产 1次与流产 2次相比 ,血清中 EMAb阳性率无明显差异 (P>0 .0 5) ;流产 3次或 3次以上与流产 1～ 2次相比 ,血清 EMAb阳性率明显降低 (P<0 .0 5)。提示 EMAb是早期自发流产的一个重要原因 ,且对流产 1～ 2次者影响较为明显。     

来曲唑与尿促性素在多囊卵巢综合征不孕患者促排卵中的应用

目的 分析来曲唑（letrozole,LE）与尿促性素（human menopausal gonadotmpin,HMG）在多囊卵巢综合征（polycystic ovary syndrome,PCOS）不孕患者促排卵中的疗效。方法 选取112例多囊卵巢综合征不孕患者,分为A、B两组,A组（来曲唑组）、B组（来曲唑与尿促性素组）,B组HMG用法为隔日肌内注射1次。观察两组成熟卵泡数、成熟卵泡率、排卵率、临床妊娠率、异位妊娠数及早期流产率等。结果 两组基础性激素水平、BMI、成熟卵泡数、排卵率差异均无统计学意义（P>0.05）;妊娠率两组分别为26.7%和41.6%,差异有统计学意义（P=0.046）,说明B组临床妊娠率优于A组;B组中HMG的周期平均用量为5.12支（532/104）。A组3例早期流产,3例多胎妊娠;B组2例自然流产,1例异位妊娠,5例多胎妊娠,差异无统计学意义（P>0.05）。结论 对于多囊卵巢综合征不孕患者进行促排卵治疗,LE与HMG联合应用且HMG隔日肌内注射1次的方案优于单用LE。     

M-MDSC在非霍奇金淋巴瘤中表达及临床意义

目的 探讨单核细胞型髓系来源抑制性细胞(M-MDSC)在非霍奇金淋巴瘤(NHL)患者中的表达水平以及临床意义.方法 选取2017年9月至2020年1月解放军第九○一医院和安徽医科大学第二附属医院35例初诊NHL患者作为试验组,同期选取20例健康志愿者作为对照组.比较两组研究对象M-MDSC、精氨酸酶-1(Arg-1)mRNA及诱导型一氧化氮合酶(iN-OS)mRNA的表达水平.分析M-MDSC与NHL患者年龄、性别、临床分期、病理分型、乳酸脱氢酶(LDH)以及IPI评分等临床指标的相关性.结果 试验组患者M-MDSC表达水平为(32.64±11.23)％,高于对照组,差异有统计学意义(P<0.05).Ⅲ～Ⅳ期、LDH升高及IPI评分为3～5分的NHL患者,M-MDSC水平升高,差异有统计学意义(P<0.05).试验组患者Arg-1水平为(18.12±5.25)ng/mL,iNOS mRNA相对水平为(12.36±3.68),均高于对照组,差异有统计学意义(P<0.05).结论 M-MDSC参与NHL患者发病过程,与疾病进展密切相关,可能成为治疗NHL的新靶点.     

基于深度教学理念在眼战伤早期急救护理教学中的应用研究

目的 探讨深度教学理念引导下的眼战伤早期急救护理教学应用效果。方法 64名参加眼战伤早期急救护理培训的眼科护士被分为两组,对照组26人,观察组38人。对照组理论部分采用清单式自主学习,操作技能采用传统示教法;观察组基于深度教学的理念,从促进理解、启发反思、沉浸式体验3个维度进行教学设计并实施理论和实践教学。对比分析两组护士一般资料和实施前、后理论成绩,操作成绩及护士核心能力得分情况。采用SPSS 22.0进行t检验和卡方检验。结果 两组护士在年龄、眼科工作年限、学历、职称等一般资料上差异无统计学意义。实施前两组护士理论成绩、操作成绩和护士核心能力得分比较差异均无统计学意义;实施后观察组护士理论成绩[（90.13±5.87） vs. （81.73±4.68）,P<0.001],两项操作成绩[（95.63±2.81） vs. （87.31±4.51）,P<0.001];[（96.24±2.74） vs. （89.08±4.50）,P<0.001]均优于对照组;观察组护士核心能力量表的评判性思维得分[（34.00±1.93） vs. （30.58±3.01）,P<0.001]和临床护理能力得分[（32.13±1.65） vs. （28.35±2.28）,P<0.001]优于对照组。结论 基于深度教学理念的教学模式有助于提升眼科护士关于眼战伤早期急救的相关知识、急救技能、临床反思及护理能力,利于提高军队临床护士的思想觉悟和练兵备战意识。