Effect of thymoquinone on the lung pathology and cytokine levels of ovalbumin-sensitized guinea pigs

Different pharmacological effects from Nigella sativa have been demonstrated in guinea pig tracheal chains in previous studies. In the present study, the prophylactic effects of thymoquinone on lung pathology as well as blood IL-4 and IFN-γ levels in sensitized guinea pigs were examined. Three groups of guinea pigs sensitized to ovalbumin were given drinking water alone (group S) or drinking water containing low (LTQ) or high (HTQ) concentrations of thymoquinone (groups S + LTQ and S + HTQ). The lung pathology as well as blood IL-4 and IFN-γ levels of the sensitized and the control guinea pigs were evaluated in three sensitized and one control group (n = 8, for all groups). The lungs of the S group showed significant pathological changes (p < 0.001). Blood IL-4 and IFN-γ levels were increased in the sensitized animals compared to those of controls (p < 0.01 and p < 0.001, respectively). Treatment of the S animals with thymoquinone significantly improved their pathological changes to the lung and decreased their IL-4 levels (p < 0.05 to p < 0.001) but increased their IFN-γ levels (p < 0.001). These results showed a preventive effect of thymoquinone on lung inflammation in sensitized guinea pigs.     

Oral administration of patchouli alcohol isolated from Pogostemonis Herba augments protection against influenza viral infection in mice

Seasonal influenza A infection results in considerable morbidity and mortality. The limited efficacy of available therapeutic strategies stresses the need for development and study of new molecules against influenza virus (IFV). Patchouli alcohol (PA), the major chemical constituent of Pogostemonis Herba, was previously found to strongly inhibit influenza H1N1 replication in vitro. In the present study, the in vivo anti-IFV effect of PA was investigated. In a mouse model infected with lethal levels of FM1, oral administration of PA (20 mg/kg to 80 mg/kg) for 7 d post IFV infection significantly increased the survival rate and survival time. For IFV infection at nonlethal levels, the quantity of IFV in the lungs 5 d after infection was significantly reduced after PA (20 mg/kg to 80 mg/kg) administration. Anti-IFV IgA, IgM, and IgG titers in serum on day 6 were significantly higher in the PA-treated group than the IFV-control group. Anti-IFV immune response augmentation was further confirmed by the elevated production of CD3+, CD4+, and CD8+ T cell levels in blood. Furthermore, the levels of inflammatory cytokines, including TNF-alpha, IL-10 and IFN-gamma in serum of mice, were regulated. Lung inflammation was reduced significantly after PA administration, and the effect may be mediated, at least in part, by regulating the lung levels of inflammatory cytokines. Thus, oral administration of PA appears to be able to augment protection against IFV infection in mice via enhancement of host immune responses, and attenuation of systemic and pulmonary inflammatory responses.     

急性白血病患者血清IFN-γ、TGF-β、IL-6和IL-17水平及临床意义

目的 研究急性白血病患者血清干扰素-γ(IFN-γ)、转化生长因子-β(TGF-β)、白介素-6(IL-6)和IL-17表达情况以及临床意义.方法 选取2014年4月-2016年3月收治的96例急性白血病患者为观察对象,按FAB分型标准分为急性淋巴细胞白血病(ALL)组35例,急性髓细胞白血病(AML)组61例.另选同期健康体检者96例为对照组.采用酶联免疫吸附法检测急性白血病患者治疗前后及对照组血清IFN-γ、TGF-β、IL-6和IL-17的表达情况.结果 治疗前,ALL组与AML组血清IFN-γ水平明显低于对照组(P＜0.05),TGF-β、IL-6、IL-17水平明显高于对照组(P＜0.05).ALL组血清IFN-γ与IL-6水平高于AML组(P＜0.05).ALL组和AML组治疗后,完全缓解患者血清IFN-γ水平高于未缓解患者(P＜0.05),TGF-β、IL-6和IL-17水平低于未缓解患者(P＜0.05).ALL组和AML组中,IFN-γ与TGF-β、IL-6和IL-17呈负相关(P＜0.05),TGF-β与IL-6和IL-17呈正相关(P＜0.05),IL-6与IL-17呈正相关(P＜0.05).结论血清IFN-γ 、TGF-β、IL-6和IL-17与急性白血病的发生、发展有关,可作为临床诊断急性白血病与评价其预后的重要指标.     

Oral administration of heat-killed Lactobacillus pentosus strain b240 augments protection against influenza virus infection in mice

Host-defense mechanisms against influenza virus (IFV) infection involve both innate and acquired immunities. Among other components, secretory immunoglobulin A (SIgA) in the airway mucosa plays a particularly pivotal role in preventing IFV infection. Among 150 strains of lactic acid bacteria, Lactobacillus pentosus strain b240 (b240) has the highest IgA-inducing potency in mouse Peyer's patch cells. We previously reported a practical new finding that oral ingestion of nonviable heat-killed b240 elevates salivary IgA secretion in humans. The present study aimed to determine if nonviable b240 can prevent IFV infection in mice. In a BALB/c mouse model infected with lethal levels of IFV A/PR8/34 (H1N1), oral administration of b240 for 3 weeks by gavage prior to IFV infection significantly prolonged the survival period. For IFV infection at nonlethal levels, the infectious titers of IFV in the lungs 7 days after infection were significantly reduced after similar b240 administration. Both anti-IFV IgA and immunoglobulin G titers in bronchoalveolar lavage fluid and plasma on day 7 were significantly higher in the b240-treated group than the control group. The augmentation of the anti-IFV immune response by b240 application was preliminarily confirmed by the elevated production of IFV-driven T-cell factors during mixed lymphocyte reactions with b240-primed splenocytes. These results suggest that oral nonviable heat-killed b240 intake can facilitate protection against IFV infection.     

Efficacy of oral administration of heat-killed probiotics from Mongolian dairy products against influenza infection in mice: alleviation of influenza infection by its immunomodulatory activity through intestinal immunity

Some probiotics possess immunomodulatory activities and have been used as complementary and alternative medicines. We previously found that 10 lactic acid bacteria (LAB) strains isolated from traditional Mongolian dairy products showed probiotic potential in vitro. In this study, we assessed the immunomodulatory activity of 10 LABs on influenza virus (IFV) infection in relation to their efficacies in IFV-infected mice. In an intranasal IFV infection model in mice, oral administration of boiled Lactobacillus plantarum 06CC2 strain (20 mg/mouse), one of the 10 LABs, twice daily for 10 days starting two days before infection was significantly effective in protecting the body weight loss of infected mice, reducing virus yields in the lungs on days 2, 4, and 6 after infection, and prolonging survival times without toxicity. The total numbers of infiltrated cells in the bronchoalveolar lavage fluid (BALF), especially macrophages and neutrophils, were significantly reduced by 06CC2 administration on day 2. On day 2, tumor necrosis factor (TNF)-α production in BALF was also reduced significantly, but interferon-α, interleukin-12, and interferon-γ productions were augmented and natural killer (NK) cell activity was significantly elevated. Furthermore, the gene expressions of interleukin-12 receptor and interferon-γ in Peyer's patches were augmented by 06CC2 administration on day 2. Thus, 06CC2 was suggested to alleviate influenza symptoms in mice in correlation with the augmentation of NK cell activity associated with the enhancement of interferon-α and Th1 cytokine productions through intestinal immunity and the reduction of TNF-α in the early stage of infection.     

Nasal priming with immunobiotic lactobacilli improves the adaptive immune response against influenza virus

The nasal priming with Lactobacillus rhamnosus CRL1505 modulates the respiratory antiviral innate immune response and improves protection against influenza virus (IFV) challenge in mice. However, the potential beneficial effect of the CRL1505 strain on the adaptive immune response triggered by IFV infection or vaccination was not evaluated before. In this work, we demonstrated that nasally administered L. rhamnosus CRL1505 is able to improve both the humoral and cellular adaptive immune responses induced by IFV infection or vaccination. Higher levels of IFV-specific IgA and IgG as well as IFN-γ were found in the serum and the respiratory tract of CRL1505-treated mice after IFV challenge. Lactobacilli treated mice also showed reduced concentrations of IL-17 and improved levels of IL-10 during IFV infection. The differential balance of inflammatory and regulatory cytokines induced by L. rhamnosus CRL1505 contributed to the protection against IFV by favoring an effective effector immune response without inducing inflammatory-mediated lung damage. The optimal immunomodulatory effect of the CRL1505 strain was achieved with viable bacteria. However, non-viable L. rhamnosus CRL1505 was also efficient in improving the adaptive immune responses generated by IFV challenges and therefore, emerged as an interesting alternative for vaccination of immunocompromised hosts. Similar to other immunomodulatory properties of lactobacilli, it was shown here that the adjuvant effect in the context of IFV vaccination was a strain dependent ability, since differences were found when L. rhamnosus CRL1505 and the immunomodulatory strain L. rhamnosus IBL027 were compared. This investigation represents a thorough exploration of the role of immunobiotic lactobacilli in improving humoral and cellular adaptive immune responses against IFV in the context of both infection and vaccination.     

紫花牡荆素对佐剂性关节炎小鼠足肿胀度和细胞因子的影响

目的研究紫花牡荆素对佐剂性关节炎小鼠血浆炎症相关细胞因子的作用及其与足跖肿胀度的相关性。方法 36只Bal b/c小鼠,随机分为6组:空白对照组、模型组、阳性药物组、紫花牡荆素12.5,25.0和50.0mg·kg~(-1)剂量组;小鼠右足垫皮内注射弗氏完全佐剂后连续灌胃给药12d,每日1次。第13天测量小鼠后足爪的肿胀度,第14天采血,用流式细胞仪的微球阵列(CBA)法检测佐剂性关节炎小鼠外周血中的致炎性细胞因子(IL-2、IFN-γ和TNF-α)和抗炎性细胞因子(IL-4和IL-5)的水平。结果紫花牡荆素可剂量依赖性地抑制Bal b/c小鼠后足爪的肿胀度;明显降低致炎性Th1细胞因子IL-2、IFN-γ和TNF-α的水平,显著提高抗炎性Th2细胞因子IL-4和IL-5的水平;小鼠后足爪肿胀度与血清细胞因子IL-2、IFN-γ和TNF-α的水平呈正相关,与IL-4和IL-5的水平呈负相关。结论紫花牡荆素改善佐剂性关节炎小鼠足跖肿胀度,可能是通过增加血液IL-4和IL-5含量,降低IL-2、IFN-γ和TNF-α含量,调节细胞因子平衡的结果。     

金舒喜辅助治疗子宫颈CINⅠ-Ⅲ对阴道微生态及阴道灌洗液IL-10、IFN-γ水平的影响观察

探讨金舒喜辅助治疗宫颈上皮内瘤变(CIN)Ⅰ-Ⅲ对阴道微生态及阴道灌洗液白介素-10(IL-10)、干扰素-γ(IFN-γ)的影响。将该院于2017年8月~2019年3月接诊的76例CIN患者随机均分为研究组及对照组,研究组(38例)给予辛复宁+金舒喜治疗,对照组(38例)仅给予辛复宁治疗,比较两组患者治疗3个月后人乳头瘤病毒(HPV)感染和CIN疗效及阴道微生态、阴道灌洗液IL-10、IFN-γ等情况。研究组HPV感染和CIN疗效均明显优于对照组(P<0.05);两组治疗后白带量、色泽、质地和气味均明显改善(P<0.05),阴道病原体检出率和阴道灌洗液IL-10水平均显著降低(P<0.05),乳酸杆菌检出率、阴道p H<4.5比例和阴道灌洗液IFN-γ水平均显著增加(P<0.05);研究组临床症状、阴道微生态、阴道灌洗液IL-10、IFN-γ水平变化幅度均大于对照组(P<0.05)。金舒喜辅助治疗子宫颈CINⅠ-Ⅲ疗效确切,可有效改善患者阴道微生态,调节阴道内IL-10、IFN-γ水平。     

Matrine ameliorates spontaneously developed colitis in interleukin-10-deficient mice

Interleukin-10 (IL-10)-deficient mice spontaneously develop T cell-mediated colitis. Previous reports have shown that Matrine may reduce the symptoms of acute colitis induced by trinitrobenzene sulfonic acid (TNBS). However, whether Matrine impacts chronic colitis remains unknown. In this study, we investigated whether Matrine could limit the symptoms of spontaneously developed colitis and its potential molecular mechanisms. IL-10 deficient mice were given Matrine or a PBS control by oral gavage daily for 4 weeks and were euthanized at week 2 or week 4. We measured body weight, colon length and weight, and histological scores. We also evaluated the spontaneous secretion of IL-12/23p40, IFN-γ and IL-17 in colon explant cultures as well as IFN-γ and IL-17 secretion in unseparated mesenteric lymph node (MLN) cells, and assessed IFN-γ, IL-17, IL-1β and IL-6 mRNA expression in colon tissue. In addition, we analyzed the proportions of CD4-positive and CD8-positive cells in unseparated MLN cells. Our results show that Matrine-treated mice exhibited better body weight recovery than controls and that histological scores and spontaneously secreted IL-12/23p40, IFN-γ and IL-17 in colon tissue were significantly decreased in treated mice compared with controls. The proportion of CD4-positive cells of MLNs in treated mice was significantly smaller than that in controls at week 4. Both cytokine production and mRNA expression of IFN-γ and IL-17 were significantly reduced in treated mice compared with controls. Taken together, our results indicate that Matrine may ameliorate spontaneously developed chronic colitis and could be considered as a therapeutic alternative for chronic colitis.     

动态检测慢加急(亚急)性肝衰竭病人细胞因子的临床意义

目的 动态观察慢加急(亚急)性肝衰竭(ACLF)的白细胞介素-2(IL-2)、白细胞介素-4(IL-4)、白细胞介素-6(IL-6)、白细胞介素-10(IL-10)、γ干扰素(IFN-γ)、肿瘤坏死因子-α(TNF-α)在不同病程时期(第0周、第8周)的变化,分析其临床意义.方法 采用酶联免疫吸附法(ELISA),检测急性肝衰竭组(ACLF)、慢性乙型肝炎组(CHB)、健康对照组的血清,第0周与治疗后第8周与其他两组进行比较.结果 (1)治疗前(第0周),ACLF组的IL-2、IL-4、L-6、IL-10、IFN-γ、TNF-α水平均高于CHB、对照组,差异有统计学意义;(2)ACLF组经过治疗第8周时,ACLF组的IL-2、IL-4、IL-6、IL-10、IFN-γ、TNF-α水平均有显著下降,但ACLF组水平仍高于CHB组和对照组;(3)存活组与死亡组比较,死亡组病人IL-2、IL-4、IL-6、IFN-γ、TNF-α水平高于存活组,差异有统计学意义(P<0.01);死亡组病人IL-10水平低于存活组,差异有统计学意义(P<0.05);两组病人的IFN-γ水平差异无统计学意义.结论 动态检测ACLF病人的IL-2、IL-4、IL-5、IL-6、IL-10、IFN-γ、TNF-α水平,对病情轻重及转归有一定指导意义.     

小鼠腹腔注射流感病毒后外周血IFN-γTNF-α和IL-2的变化及意义

目的 观察和比较小鼠腹腔注射人流感病毒H3N2和禽流感病毒H9N2后外周血干扰素γ(interferon-γ,IFN-γ)、肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)和白细胞介素2(interleukin-2,IL-2)质量浓度的变化,探讨其变化规律.方法 采用腹腔注射方式给小鼠注射人流感病毒H3N2、禽流感病毒H9N2或无病毒尿囊液,分离血清,用ELISA法检测注射后第1、2、3、4和5天外周血IFN-γ、TNF-α和IL-2质量浓度.分别比较人流感病毒H3N2和禽流感病毒H9N2注射组与阴性对照组在不同时间的IFN-γ、TNF-α和IL-2质量浓度.用方差分析和LSD-t检验进行统计学分析.结果 在某些时间段,病毒注射组外周血IFN-γ和IL-2质量浓度与阴性对照组问的差别有统计学意义,而病毒注射组TNF-α质量浓度与阴性对照组间的差别则无统计学意义.结论 小鼠腹腔注射人流感病毒H3N2和禽流感病毒H9N2可促进IFN-γ和IL-2产生,而对TNF-α产生则无显著影响.     

下生殖道感染患者阴道灌洗液中干扰素-γ和白细胞介素-4的表达

目的研究下生殖道感染患者阴道局部干扰素(IFN)-γ和白细胞介素(IL)-4水平及其与疾病发生的关系。方法应用酶联免疫吸附法测定健康妇女24名(健康对照组)、外阴阴道假丝酵母菌病(VVC)患者35例(VVC组)、细菌性阴道病(BV)患者30例(BV组)、宫颈人乳头瘤病毒(HPV)感染37例(HPV组)的阴道灌洗液IFN-γ,IL-4水平。结果①IFN-γ:VVC组与健康对照组IFN-γ水平比较,差异无统计学意义(P>0.05);BV组和HPV组分别与健康对照组比较,差异均有统计学意义(P<0.05);而VVC组与BV组比较差异无统计学意义(P>0.05);VVC组与HPV组比较差异均有统计学意义(P<0.05);BV组与HPV组比较,差异无统计学意义。②IL-4:VVC组与健康对照组比较,差异有统计学意义(P<0.05);BV组和HPV组分别与健康对照组比较差异均无统计学意义(P>0.05);VVC组分别与BV组及HPV组比较,差异有统计学意义(P<0.05);HPV组与BV组比较则差异无统计学意义(P>0.05)。结论阴道局部免疫功能异常可能与下生殖道感染的发生有关,保持免疫因子间的平衡,是防止下生殖道感染的重要因素;检测其阴道灌洗液中的细胞因子,可以反映其阴道局部免疫状况,并作为治疗监测的指标之一。     

实验性矽肺大鼠肺组织中Th1/Th2型细胞因子的变化及意义

目的研究实验性矽肺大鼠在染尘后不同时间肺组织中Th1型细胞因子IFN-γ和Th2细胞因子IL-4的变化,初步探讨Th1/Th2型细胞因子与矽肺发病机制的关系。方法将30只健康雄性SD大鼠随机分为模型组18只、对照组12只。模型组以非暴露方式气管内一次性注入1 ml二氧化硅悬液（40 g·L^-1）建立大鼠矽肺模型,对照组气管内注入等量的灭菌生理盐水。分别在染尘后第1、7、14、21、28、35天各处死3只模型组和2只对照组大鼠,免疫组化SABC法观察肺组织中IFN-γ和IL-4的表达情况,用图像分析系统进行定量分析。结果与对照组相比,染尘后第1、7、14、21天,模型组大鼠肺组织中IFN-γ表达明显增强（P〈0.05）;IL-4的表达显著减少（P〈0.05）。染尘后第28、35天,模型组大鼠肺组织中IFN-γ表达与对照组没有显著性差异（P〉0.05）;而IL-4的表达量大量增加,明显高于对照组（P〈0.05）。结论矽肺发展早期以Th1型细胞因子发挥细胞免疫调节作用为主;后期Th2型细胞因子分泌增加,体液免疫增强。     

I型糖尿病不同病情血清IL-6、TNF—α、IFN—γ的变化及临床意义

目的探讨TNF-α、IFN-γ和IL-6促进I型糖尿病发生的机制。方法采用酶联免疫吸附法对新发病患者组（A组）19例、I型糖尿病并发酮症酸中毒组（B组）14例、I型糖尿病合并有慢性并发症组（C组）12例和正常对照组22例的血清IL-6、TNF-α和IFN—γ的水平进行测定。结果（1）A组、B组和C组治疗前的TNF—α、IFN-γ水平显著高于正常对照组（P〈0．01）;C组IFN-γ、TNF-α和IL-6水平高于A组、C组,差异有统计学意义（P〈0．05）;（2）A组给予胰岛素治疗血糖稳定3个月后TNF—α、IFN-γ下降,但仍高于对照组（P〈0．05）;C组高于A组、B组;（3）B组给予胰岛素治疗,血糖稳定2周后TNF-α、IFN-叫稍有下降,但较对照组仍高（P〈0．01）,C组给予胰岛素治疗1月后有下降,但仍显著高于正常对照组（P〈0．01）;（4）C组IL-6水平明显升高,在治疗后下降并接近正常对照组（P〈0．05）。结论（1）TNF-α、IFN-γ水平的升高可能是I型糖尿病的发病机制之-;（2）TNF-α、IFN-γ水平与I型糖尿病患者的病程有关,并可作为评价I型糖尿病的疗效指标之-;（3）在TIDM合并有慢性并发症患者中不仅存在TNF—α、IFN-γ水平的异常。同时也存在IL-6水平的异常,IL-6水平的升高有可能是TIDM发生慢性并发症机制之一。     

ITP中IFN-γ和IL-4 mRNA表达与其甲基化水平的相关性研究

目的:检测免疫性血小板减少症(ITP)患者的干扰素(IFN)-γ和白细胞介素(IL)-4的mRNA表达水平及其各自基因启动子区DNA甲基化的水平.方法:取36例ITP患者(ITP组)和36例正常对照(对照组)外周血,采用实时定量PCR方法检测IFN-γ和IL-4的mRNA水平;随机抽取2组中的各10例,采用DNA甲基化修饰后测序的方法检测IFN-γ和IL-4基因启动子区的甲基化水平,并计算IFN-γ和IL-4基因的mRNA水平与其基因启动子区DNA甲基化水平的相关性.结果:与对照组相比,ITP组IFN-γ的mRNA水平增高(1.86±0.29 vs 0.83±0.14,P＜0.05),而IL-4的mRNA水平降低(0.78±0.22 vs 1.45±0.40,P＜0.05),Th1/Th2(IFN-γ/IL-4)比值升高(7.11±0.60vs 3.12±1.88,P＜0.01).2组IFN-γ和IL-4基因CpG岛的位点甲基化水平和整体甲基化水平差异均无统计学意义(P＞0.05).ITP组中IFN-γ和IL-4的mRNA表达水平与基因启动子区甲基化水平不存在相关性(均P＞0.05).结论:ITP患者处于Th1极化状态,IFN-γ和IL-4的基因启动子区的甲基化水平对各自基因mRNA水平的表达不起调控作用.     

呼吸道合胞病毒肺炎患儿急性期血清IFN-γ水平与病情相关性研究

目的观察呼吸道合胞病毒（RSV）肺炎患儿急性期血清中IFN-γ水平的变化和病情严重程度、过敏状态的关系,为临床上使用干扰素治疗重症RSV感染提供理论依据。方法实验组为RSV阳性的肺炎患儿42例;以患儿入院时的临床表现,将患儿分为轻度组和重度组其余为轻度,其中轻度组33例,重度组9例;根据患儿的过敏状况（如湿疹史）及家族过敏史（哮喘、过敏性鼻炎、过敏性结膜炎、特应性皮炎等）分为具有过敏因素组29例和无过敏因素组13例。对照组38例为年龄在2岁以内,同时期在医院外科住院,近1周内无呼吸道感染的患儿。用ELISA法检测血清中IFN-γ的浓度。结果实验组IFN-γ浓度与对照组比较,差异无统计学意义（P〉0.05）。实验组中轻度组IFN-γ浓度与重度组比较差异有统计学意义（P〈0.05）;而具有过敏因素组IFN-γ浓度与无过敏因素组比较差异无统计学意义（P〉0.05）。结论 RSV感染早期血清中IFN-γ的浓度与正常婴幼儿无明显差异;IFN-γ的水平与疾病的严重程度呈现明显的负相关,重度患儿IFN-γ浓度显著低于轻度患儿,对重度的RSV肺炎患儿,使用干扰素是合理的;RSV感染时IFN-γ的浓度与过敏因素之间未发现有显著的联系。     

孟鲁斯特钠对毛细支气管炎患儿Th1/Th2细胞平衡的影响

目的 观察孟鲁司特钠对毛细支气管炎患儿Th1/Th2细胞平衡的影响.方法 采用酶联免疫吸附试验（ELISA）法分别测定30例毛细支气管炎患儿（毛支组）口服孟鲁司特钠片治疗前后及10例门诊健康体检婴幼儿（对照组）血清白介素（IL）-4及γ-干扰素（IFN-γ）水平.结果 毛支组患儿治疗前血清IL-4水平明显高于对照组,差异有统计学意义（P〈0.05）,而IFN-γ水平与对照组相似,差异无统计学意义（P〉0.05）;治疗后血清IL-4水平及IL-4/IFN-γ比值较治疗前显著降低,差异有统计学意义（P〈0.05）,而血清IFN-γ水平与治疗前相似,差异无统计学意义（P〉0.05）.结论毛细支气管炎患儿Th1/Th2细胞处于失衡状态,白三烯受体拮抗剂--孟鲁司特钠可明显调节毛细支气管炎患儿Th1/Th2细胞失衡,预防喘息反复发作.     

子痫前期血清IFN-γ、IL-6的水平与患者病情变化的相关性研究

目的探讨子痫前期血清IFN-γ、IL-6的水平与患者病情变化的相关性。方法 Elisa法检测血清IFN-γ、IL-6在30例轻度子痫和28例重度子痫前期及35正常孕妇中的水平。结果轻、重度组子痫前期患者血清IFN-γ水平显著高于正常对照组(P<0.05),重度组血清IFN-γ高于轻度组,差异具有显著性(P<0.05);血清IL-6的水平随着患者病情加重而呈降低趋势,但轻、重度组与正常对照组比较无统计学差异(P>0.05).血清IFN-γ/IL-6比值与患者病情呈正相关(P<0.05)。结论 IL-6和IFN-γ在妊娠期高血压疾病的发生、发展中发挥重要作用。     

儿童哮喘IL-4、IFN-γ与总IgE关系的研究

目的考察儿童哮喘IL-4、IFN-γ与总IgE的相关性。方法采用了双抗体夹心ELISA法、免疫比浊法对60例支气管哮喘儿童(观察组)与30例无气喘与特异体质住院的支气管炎、支气管肺炎儿童(对照组)的血清IL-4、IFN-γ与总IgE含量进行测定并分析。结果观察组IL-4、总IgE水平显著高于对照组(P<0.01),而IFN-γ水平显著低于对照组(P<0.01),组间差异具有统计学意义,直线回归分析结果显示,观察组IL-4与总IgE表现为正相关(r=0.884,P<0.01),IFN-γ与总IgE表现为负相关(r=0.759,P<0.01);对照组IL-4与总IgE表现为正相关(r=0.887,P<0.01),IFN-γ与总IgE表现为负相关(r=0.741,P<0.01)。结论早期就儿童血清IL-4、IFN-γ以及IgE水平实施监测对防治无气喘与特异体质的支气管炎、支气管肺炎向哮喘发展具有十分重要的临床意义,而通过研发改善Th1/Th2平衡的新型免疫调节剂是治疗儿童哮喘的可靠方法。     

清热祛湿法抗流感病毒的实验研究

目的 观察清热祛湿法对流感病毒所致小鼠病毒性肺炎的作用,通过探讨清热祛湿药物的抗流感病毒免疫调节机制,为指导临床辨证治疗流感提供实验依据.方法 （1）观察清热祛湿法体外抗病毒作用及对小鼠流感病毒性肺炎的影响.（2） 用FM1株甲1型流感病毒感染小鼠.制作病毒性肺炎模型,将小鼠随机分为正常组、模型组、蒿芩清胆汤组、病毒唑对照组,分别用单标法流式细胞仪及ELISA法测定四组小鼠外周血T淋巴细胞哑群（（CD3/CD4、CD8）百分比及比值及血清IL-4、IL-18、IFN-γ的含量.结果 清热祛湿法在体外对流感病毒FM1株无明显的抑制作用,但对小鼠流感病毒性肺炎均有抑制作用,与模型对照组比较,差异有显著性意义（P〈0.05）,与病毒唑组比较,差异无显著性意义（P〉005）蒿芩清胆汤对小鼠感染流感病毒FM1后血中的CD3＋CD4＋百分比和CD3＋CD4＋/CD3＋CD8＋比值下降有一定的抑制作用并且能够降低模型小鼠血清TIL-18及IFN-r的值.结论 清热祛湿药物对流感病毒具有抑制作用,但不是直接杀死流感病毒,而是一方面通过调整T细胞亚群的百分率或CD4＋CD4＋/CD4＋CD8＋比值,另一方面通过降低IFN-r、IL-18表达水平,对流感病毒感染引起的细胞免疫功能降低有一定的抑制作用,从而减少异常的细胞免疫反应,调节和加强体液免疫功能,有利于损伤组织的修复.