女性生殖道解脲脲原体感染与细菌性阴道病的关系

目的探讨解脲脲原体(UU)感染和细菌性阴道病(BV)的相互关系。方法对本院性病门诊2008年6月～2009年1月本院性病门诊就诊的1491例患者进行了UU和BV的检测。UU阳性的696例纳入观察组,UU阴性795例作为对照组,调查两组BV的阳性率,并对年龄、职业分布、避孕方式和阴道清洁度等因素进行分析。结果观察组BV的阳性率(22.41%)明显高于对照组BV的阳性率(11.45%);观察组BV的阳性率先随着年龄的增加而增高,至31～40岁到达高峰,随后又随着年龄的增长而降低,对照组中BV的阳性率随着年龄的增长而降低,与年龄呈负相关;职业分布在两组间差异无显著性意义。观察组中采用屏障避孕法BV的检出率明显比非屏障避孕法低。观察组BV阳性患者以阴道清洁度Ⅲ度和阴道清洁度Ⅳ度为主,对照组中BV患者以阴道清洁度Ⅱ度为主。结论女性生殖道UU感染可以增加BV的发病率;屏障避孕法可以降低UU患者BV的发病率;女性生殖道UU感染合并BV感染时,阴道清洁度下降。     

生殖支原体、解脲脲原体与泌尿生殖道症状的相关性研究

目的：探讨泌尿生殖道症状与生殖支原体（MG）与解脲脲原体（UU）感染的相关性。方法：分别用聚合酶链式反应法、培养法检测我院性病门诊就诊患者的泌尿生殖道分泌物标本中的MG和UU。结果：男性有临床非淋菌性尿道炎（NGU）症状者和无NGU症状者MG的阳性率分别为6.86%和0%,UU阳性率分别为8.92%和13.92%,两组无明显差异。女性有临床NGU症状者和无NGU症状者MG的阳性率分别为3.13%和0%,UU阳性率分别为18.75%和45.45%。结论：MG可能是NGU的一个致病菌,而UU可能与NGU无明显相关性。     

女性下生殖道假丝酵母菌感染患者解脲脲原体与沙眼衣原体混合感染情况研究

目的:探究并分析女性下生殖道假丝酵母菌(VVC)感染患者解脲脲原体(UU)与沙眼衣原体(CT)混合感染情况。方法:选取我院自2012年12月至2014年12月收治的下生殖道假丝酵母菌感染患者103例作为病例组,并征集于我院体检的健康女性250例,作为对照组。将病例组分为单纯组、重度组以及复发组。对所有患者进行UU、CT阳性率检查,并分别记录下UU阳性率、CT阳性率以及UU混合CT阳性率。结果:单纯组、重度组、复发组患者之间UU阳性、CT阳性、UU混合CT阳性率无明显差异(P>0.05),无统计学意义。对照组患者UU阳性为8.4%,病例组患者UU阳性为57.2%,明显高于对照组患者(χ2(病,对)=17.134,P=0.002),具有统计学意义。单纯组、重度组、复发组患者均明显高于对照组患者UU阳性率(χ2(单,对)=8.932,P=0.004;χ2(重,对)=8.362,P=0.005;χ2(复,对)=4.567,P=0.034),具有统计学意义。结论:患有VVC的女性常伴有生殖道UU感染,两者有着密切的联系,但VVC的严重程度与女性生殖道UU感染无明显联系,而CT感染与VVC的发生无明显相关性,尚需进一步研究。     

女性生殖道解脲脲原体感染相关因素调查

为调查女性生殖道解脲脲原体(UU)感染现状及影响因素,设计结构式问卷,知情同意后收集女性性行为学资料,阴道拭子检测白念珠菌、加特纳菌、毛滴虫.宫颈拭子培养淋球菌、UU、酶免疫测定(EIISA)法检测沙眼衣原体抗原.结果:接受调查的715例女性中,321例（44.9%）宫颈UU阳性,其中142例（44.2%）为UU单纯感染,179例（55.8%）为UU感染,合并各种阴道炎,其中合并白念珠菌性阴道炎21例、滴虫性阴道炎21例、细菌性阴道病47例和非特异性阴道炎90例,分别占相应阴道炎的57%、81%、69%和42%.     

男性尖锐湿疣患者合并解脲支原体感染的临床特点分析

目的:探讨男性尖锐湿疣患者合并解脲支原体感染的临床特点分析。方法:选择86例男性尖锐湿疣患者为观察组,86例健康男性为对照组,根据患者主诉记录临床特征,采用醋酸白试验和UU培养基检测判定复发情况。结果:86例CA患者中,共41例患者检出解脲支原体感染,占47.7%。86例对照组中,共9例检出解脲支原体感染,占10.5%;二者检出率比较具有显著性差异(P0.05);单纯CA组患者复发7例,复发率为15.6%;CA合并UU组患者复发16例,复发率为39.0%,二者复发率比较具有显著性差异,合并UU感染更易复发;41例UU阳性患者中,21例患者出现尿道口黏膜明显充血发红,占51.2%;24例患者镜检出现多形核白细胞,占58.5%;5例患者主诉提及尿急、尿频、尿道不适,尿道分泌物等尿道炎症状,占12.2%。结论:尖锐湿疣合并解脲支原体感染是导致尖锐湿疣复发的重要因素,需尽早进行常规检查和化验,并进行规范化治疗。     

350例盆腔炎患者解脲支原体感染情况分析

目的:了解盆腔炎(PID)患者解脲支原体(UU)感染情况,为盆腔炎的临床防治提供有参考价值的实验依据。方法:采集PID患者和正常女性的宫颈分泌物,体外培养法检测两组UU的感染率,统计分析两者UU感染率的差异。结果:350例PID患者中UU阳性率为50.2%;对照组130例中UU阳性率为14.6%,两组UU感染率比较有统计学意义(Z=7.07,P<0.05)结论:盆腔炎患者的UU感染率较高,防治UU感染,可能有益于预防盆腔炎的发生。     

沙眼衣原体与解脲支原体感染与女性不孕症相关性分析

目的:分析探讨沙眼衣原体(CT)与解脲支原体(UU)感染与女性不孕症之间的关系,以指导临床治疗。方法:收集我院自2013年12月至2015年6月治疗的不孕症患者235例,其中原发不孕组127例,继发不孕组108例。收集同期已生育的健康查体女性105例作为对照组。采用荧光定量PCR法进行宫颈分泌物沙眼衣原体与解脲支原体检查,并对结果进行统计学分析。结果:不孕组CT、UU及其联合感染率分别为43.4%、49.8%和13.6%,对照组感染率为10.5%、12.4%和2.9%,均有显著性差异(P<0.05)。原发不孕组CT、UU及其联合感染率分别为45.7%、46.5%和13.4%;继发不孕组CT、UU及其联合感染率分别为40.7%、53.7%和13.9%,均无显著性差异(P>0.05)。各年龄段不孕症患者感染CT、UU无显著性差异(P>0.05)。结论:沙眼衣原体与解脲支原体感染与女性不孕有一定程度的相关性,沙眼衣原体与解脲支原体检测应成为不孕症的临床常规检查项目。     

3575例性病门诊尿道(宫颈)炎患者病原体检测分析

目的:了解性病门诊尿道(宫颈)炎患者的病原体感染情况及特点。方法:对3575例性病门诊尿道(宫颈)炎患者检测其淋球菌(NG)、解脲支原体(UU)、人型支原体(MH)、沙眼衣原体(CT)的感染情况。结果:3575例患者病原体检出率为64.47%,其中男性患者检出率明显高于女性患者(P<0.001);单一病原体感染占63.99%,混合病原体感染占36.01%。结论:性病门诊尿道(宫颈)炎患者混合病原体感染率较高,提示应将NG、UU、MH、CT作为该类病人常规的实验室检查项目。     

4030例性病门诊患者沙眼衣原体、淋球菌及解脲脲原体感染情况分析

目的了解性病门诊患者沙眼衣原体(CT)、淋球菌(NG)及解脲脲原体(UU)感染情况。方法回顾性分析2013—2016年首都医科大学附属北京地坛医院性病门诊患者CT、NG、UU核酸检测结果。结果 4 030例患者中男2 509例,女1 521例,年龄1~73岁,平均(32.73±9.06)岁。CT、NG、UU总阳性率分别为11.29%(455/4 030)、6.72%(271/4030)、26.63%(1 073/4 030)。不同年份患者NG阳性率之间差异有统计学意义,2016年NG阳性率最高。不同性别患者NG、UU阳性率之间差异有统计学意义,男性患者NG阳性率较高,女性患者UU阳性率较高。不同年龄段患者CT、NG、UU阳性率之间差异均有统计学意义。CT、NG、UU阳性患者年龄多在21~40岁,但15~20岁年龄段的患者CT、NG、UU阳性率均最高。CT阳性患者和NG阳性患者中混合感染的构成比分别为27.91%和24.72%。结论性病门诊患者CT、NG、UU核酸检测阳性率较高,建议性病门诊常规筛查患者CT等性病病原体感染,并在临床工作中注意各种性病病原体的混合感染。     

核酸扩增杂交梳法检测沙眼衣原体和解脲支原体感染

目的　探讨本地区性传播疾病 (STD)患者沙眼衣原体 (CT)、解脲支原体 (UU)的感染现状及使用核酸扩增杂交梳法检测CT、UU的价值。方法　使用核酸扩增杂交梳法对 2 178例STD患者的CT、UU进行检测。结果　男性CT阳性率为 8.7% ,女性为 12 .6% ;男性UU阳性率为 19.2 % ,女性为 17.1% ;混合感染男性为 3 .7% ,女性为 7.2 %。核酸扩增杂交梳与UU培养法的符合率为 97.3 %。结论　女性比男性更易感染CT ;核酸扩增杂交梳法是检测病原体DNA的快速、准确、敏感的方法     

妇科门诊人群宫颈解脲支原体检出情况及分群分型

目的 调查妇科门诊人群宫颈解脲支原体检出情况及其分群分型。方法 用液体选择培养基初筛妇科体检人群261例，初筛阳性的液体培养基用玻璃奶法提取DNA，再用PCR法进行分群分型。结果 261例中UU阳性159例（60.9%)，其中生物一群151例（95.0%)，生物二群8例（5.0%)；生物一群中血清1型30例(19.9%），血清3型62例（41.1%)，血清6型44例（29.1%)，血清14型2例（1.3%)，混合感染13例（8.6%)。结论 在妇科门诊人群中UU的检出率达到60.9%，以生物一群为主（95.0%)，而且以1、3、6型单纯感染主为（90.1%)。所以妇科门诊人群中单纯从宫颈分离出UU并不意味着致病。     

Urethritis in women attending an STD clinic.

Of an unselected group of 159 women attending a sexually transmitted diseases (STD) clinic 20% (32) had symptoms of urethritis. A positive correlation existed between the finding of more than 10 polymorphonuclear leucocytes (PMNL) per high-power field in the Gram-stained urethral smear and the presence of Neisseria gonorrhoeae, Chlamydia trachomatis, and Trichomonas vaginalis. Conversely, these organisms were rarely isolated if no PMNL were present. Fewer cultures gave positive results for these organisms if micturition had occurred less than four hours before examination. C trachomatis was recovered from the urethra or endocervix in 29/150 (19 . 3%) and from the urethra alone in six women. In contrast, N gonorrhoea was never recovered from the urethra in the absence of endocervical infection. Of the 159 women 10% had bacteriuria due to non-sexually transmissible agents; 50% had asymptomatic bacteriuria. All, however, had other urethral pathogens isolated as well. Thus, sexually transmitted disease agents are highly prevalent in women attending an STD clinic who have signs and symptoms of urethritis. As in non-gonococcal urethritis in men, C trachomatis may be an important cause of urethritis in women.     

宫颈糜烂与解脲支原体、沙眼衣原体感染关系的临床研究

目的　研究解脲支原体、沙眼衣原体感染与宫颈糜烂的关系。方法　用聚合酶链反应 (PCR)法对 3 2 4例宫颈糜烂患者 (观察组 )、3 2 4例无宫颈糜烂的宫颈炎患者 (对照组 )进行解脲支原体、沙眼衣原体的检测。结果　观察组解脲支原体、沙眼衣原体总检出率为 2 0 .68% ,显著高于对照组的 9.2 6% (P <0 .0 1) ;其中轻度糜烂患者检出率为 6.5 9% ,中度 2 7.18% ,重度 5 1.85 %。轻、中、重度宫颈糜烂各检出率经统计学处理有显著性差异 (P均 <0 .0 1) ;单纯解脲支原体检出率为 12 .65 % ,单纯沙眼衣原体检出率为 5 .2 5 % ,两者混合感染检出率为 2 78% ,解脲支原体检出率显著高于沙眼衣原体 (P <0 .0 1) ;有不洁性生活史者阳性检出率为 44 .74% ,显著高于无不洁性生活史者的 13 .3 1% (P <0 .0 1)。结论　解脲支原体、沙眼衣原体感染是引起宫颈糜烂的重要因素。     

Genital HPV infection not a local but a regional infection: experience from a female teenage group.

OBJECTIVES--To investigate the prevalence of human papillomavirus (HPV) infections in a group of female teenagers, and to analyse to what extent HPV DNA was also detectable, in urethra and cervix samples among the patients with macroscopic genital warts compared with those without. DESIGN--The patients were interviewed about their sexual habits and history of venereal diseases. They underwent a gynaecological health control examination, including macroscopic inspection for genital warts and collection of a cytological vaginal smear (Pap smear). Cell samples were also taken from endocervix and urethra and from vulva lesions, when found. These samples were tested for HPV DNA of the types 6, 11, 16, 18 and 33 using the polymerase chain reaction (PCR) technique. SETTING--An adolescence out-patient clinic in Malmö, Sweden. SUBJECTS--Forty-nine female teenagers consulting for gynaecological complaints, some of them for genital warts. RESULTS--Twenty patients had present and four had a history of genital warts (group A). The other 25 patients had no visible lesions (group B). In the first group (A) 18 of the 24 patients were positive for HPV DNA in one or more of the three locations studied. More patients were positive in urethra (17) than in cervix (15). In group B four of the 25 patients were positive for HPV DNA in urethra, three of these also in cervix. In the two groups 11 and four patients, respectively, showed pathological Pap smears. CONCLUSIONS--The finding of HPV DNA in urethra, both from women with and without visible genital warts, indicates that there is a high probability that the infection is also present in cervix, suggesting that the genital HPV infections are multifocal. Thus, patients with genital warts are most likely to have cervical HPV infections and will more often have pathological Pap smears than patients without warts.     

解脲支原体感染的流行病学调查

解脲支原体是非淋菌性尿道炎的主要病原体之一。本文探索了不同人群中解脲支原体的寄生和感染状况。在123名性乱妇女、94名妇科门诊患者和24名棉纺厂女工中,女性生殖道解脲支原体感染率分别为37.4%、20.2%和16.7%。性乱妇女解脲支原体感染率为37.4%,单一性伴妇女感染率为19.5%,差异有非常显著性意义(x~2=9.45,P<0.01)。     

慢性前列腺炎患者解脲支原体及人型支原体感染的研究

对９０例性病门诊慢性前列腺炎患者前列腺液及３６例正常对照组的前列腺液进行解脲支原体（ＵＵ）及人型支原体（ＭＨ）培养。结果表明，慢性前列腺炎组ＵＵ阳性１８例（２０．００％），ＭＨ阳性４例（４．４４％）；对照组ＵＵ、ＭＨ均为阴性。两组ＵＵ阳性率，经统计学处理有显著性差异（Ｐ〈０．０１）。作者认为，ＵＵ是性病后慢性前列腺炎的主要病原体之一。     

A comparison of an enzyme immunoassay and cell culture for detection of Chlamydia trachomatis in genito-urinary specimens

Chlamydia trachomatis infection is easy to treat but laborious to diagnose by culture. An antigen-detecting enzyme immunoassay (EIA; Chlamydiazyme, Abbott Laboratories, North Chicago, IL), suitable for testing many samples, was compared with the conventional iodine-stained, one-passage culture. A total of 471 duplicate samples from 218 men (urethra) and 128 women (urethra and cervix) attending a sexually transmitted disease clinic were examined by both tests. The overall sensitivity, specificity, and predictive value of positive and negative results were 80.3%, 96.7%, 86.4%, and 95.0%, respectively. No difference between male and female patients was observed. A remarkable difference between sensitivities of female urethral (60.0%) and cervical (95.5%) samples was found. This difference is clearly important in cases of women with urethral infection only (19% in our study) and points to the need for further improvement of this EIA.     

性传播疾病中的支原体感染

对８７２例性病门诊患者的尿道（宫颈）拭子标本进行了解脲支原体和人型支原体的培养，其中部分标本还做了淋球菌和沙眼衣原体的培养。结果表明，支原体培养阳性者共２５８例（２９．６％），其中ＵＵ感染为１８８例（１２．６％），显著高于ＭＨ感染（１７例，１．９％）和ＭＨ与ＵＵ混合感染（５３例，６．１％）（Ｐ〈０．０１）。在１７３例支原体培养阳性的患者中有５３例（３０．５％）合并ＣＴ和（或）ＮＧ感染。作者对上述病     

Group B streptococci in venereal disease clinic patients.

The isolation rates of group B streptococci for 457 men and 300 women attending a venereal disease clinic were 16-4 and 20-6% respectively. The frequencies were significantly increased by using a selective medium and by taking specimens from the female urethra as well as the cervix. In contrast to gonorrhoea the presence of group B streptococci could not be related to promiscuity. In addition, the isolation of group B streptococci was found to be independent of a concomitant gonococcal infection. This study indicates that group B streptococci have little relation to the clinical signs and symptoms of genital infection, such as dysuria, discharge and inflammatory mucosal reaction.