红景天注射液的提取与精制工艺研究

目的对红景天注射液的提取与精制工艺进行研究。方法以红景天苷和酪醇的总转移率为考察指标,采用正交实验对提取与精制工艺进行优化。结果红景天的最佳提取工艺为:将粉碎后的药材,以8倍量的水为提取溶剂,加热回流提取3次,1h/次;采用醇沉法和明胶沉淀法对红景天水提取物进行精制,醇沉法中调含醇量至70%,明胶沉淀法的最佳工艺为:将醇沉后的药液浓缩至0.5mg·ml~(-1)(相当于原生药),调pH值至5.0,在60℃,转速为60r·min~(-1)的条件下,加入5%明胶溶液至不再生成沉淀时为止。结论采用上述提取精制方法制得的红景天浸膏杂质检查符合注射液的要求,该方法可用于红景天注射液的提取与精制。     

正交试验法优选红景天的醇沉工艺

红景天为景天科植物大花红景天Rhodiloa crenulata (Hook.F.et.Thoms.)H.Ohba的干燥根和根茎.其性平、味甘、微苦,具有益气活血、通脉平喘的功效,用于气虚血瘀、胸痹心痛、中风偏瘫、倦怠气喘等症状[1].目前以红景天为原料药材的制剂的提取主要是以水作为溶媒[2-5],由于在水提取过程中,药材的一些无效成分如糊化淀粉、油脂、脂溶性色素、树脂、树胶等也随同有效成分一起溶出,为了保证制剂的质量和稳定性,需要进行醇沉除杂工艺.本试验用正交试验选择最佳醇沉工艺,以杂质重量和红景天中的红景天苷的含量为考察指标,对红景天水提浓缩液醇沉工艺进行正交试验.     

中药水提取液常用精制方法概述

近10年来,中药水提取液新的精制方法发展很快,如超滤法、大孔树脂吸附法、吸附澄清法等在中药水提取液的精制上应用逐渐广泛。基于此,笔者结合研究和应用实际,对上述方法进行介绍,供大家应用时参考。 1 醇沉法 醇沉法是水提取乙醇沉淀的略称,是一种比较好的浸提除杂方法。该法是在水提取液浓缩后,加入一定量的乙醇,将不溶于乙醇的杂质如淀粉、树胶、粘液质、蛋白质等从溶液中沉淀析出,过滤去除。醇沉法主要是除去一些水溶性杂质。因为在用水煎煮时,不仅水溶性有效成分被提取出来,而且水溶性杂质也被提出来了。醇沉法就是利用有效成分能…     

中药水提取液常用精制方法及应用概述

用水提取中药是中药复方制剂中运用最为广泛的工艺步骤。以水为溶媒廉价易得 ,设备简单 ,操作方便 ,成本低。然而 ,中药水提取液一般体积大 ,溶液中存在大量鞣质、蛋白质、粘液质、多糖、果胶等大分子物质及许多微粒、亚微粒和絮状物等 ,它们大部分不但没有药理作用 ,而且影响产品的质量 ,是造成中药“粗、大、黑”的主要原因。怎样对提取液进行科学合理的纯化、精制 ,去粗取精 ,既保留中药中的有效成分和疗效 ,又除去无效杂质 ,使中药达到现代制剂的要求 ,是目前中药新制剂研究中的一个重要课题。本文就常用的精制技术、方法及其应用作一综…     

红景天中红景天苷和多糖制备工艺研究

目的:优选红景天中红景天苷和多糖的制备工艺。方法:通过水提醇沉法,采用L9(34)正交试验法,以浸膏得率、红景天苷含量、多糖含量为评价指标,考察加水量、煎煮时间、煎煮次数对水提取工艺的影响;以红景天苷含量、多糖含量为评价指标,考察药液浓度、药液含醇量对醇沉工艺的影响。结果:以煎煮3次,每次加8倍量水,煎煮2 h为最佳提取工艺,以药液浓缩至每1 mL相当于原生药0.4 g,加95%乙醇醇沉,使药液含醇量70%为最佳醇沉工艺。结论:优选工艺可为新药研究和综合利用红景天资源提供参考。     

高山红景天酶解工艺研究

高山红景天系景天科植物高山红景天的干燥根及根茎,主要成分为红景天苷及多糖,具有抗疲劳、耐缺氧等药理作用[1,2]。为增加红景天苷转移率,该药材的提取工艺多采用醇提法[3],但醇提法存在着生产成本高,多糖保留率低等缺点。酶解法是近年来应用于中药提取的一项新技术,选用适当的酶,可较温和地将植物组织分解,加速有效成分的溶出,从而提高提取效率[4,5]。本实验选用纤维素酶,通过正交试验优选酶解法提取红景天苷的工艺条件,并与     

银黄片中金银花提取液的精制工艺研究

目的优选银黄片中金银花水提液的最佳醇沉工艺。方法采用正交实验法,以绿原酸转移率和醇沉液中绿原酸的纯度为考察指标。对影响金银花醇沉工艺的因素进行了研究。结果实验设计3因素中,醇沉时乙醇浓度与两次醇沉时药液的相对密度对实验有显著影响。结论金银花水提液的最佳醇沉工艺为:药液第1次浓缩至密度1.2,醇沉浓度65%;第2次浓缩至密度1.1,醇沉浓度75%。     

红景天提取工艺的优化

目的：优化红景天的提取工艺。方法：运用正交设计法进行实验设计，用高效液相色谱法测定提取物中红景天苷的含量。结果：确认最佳提取方案为8倍量水，提取2h／次，提取3次。结论：用上述方法可以优化提取工艺。     

我国红景天提取工艺和含量测定研究概况

本文综述近年来国内对红景天的提取工艺和含量测定的研究进展。     

双黄连的提取精制工艺研究

采用亚沸态水提，絮凝和醇沉相结合的方法实现双黄连的提取精制，达到提高中药有效成分的目的。得到最佳方案为：经超临界萃取后的100g药材，加入10倍体积的水，在90℃下煎煮2h，过滤，滤液浓缩至80mL，在50℃下，加入1．5％的絮凝剂133mL，搅拌5min，离心，上清液浓缩至16mL，加入95％乙醇至含醇量为80％，过滤，滤液蒸干，得率约为5-6％。     

The Effects of Rhodiola rosea L. Extract on Anxiety,Stress, Cognition and Other Mood Symptoms

This trial evaluated the impact of a Rhodiola rosea L. extract on self‐reported anxiety, stress, cognition, and other mood symptoms. Eighty mildly anxious participants were randomized into two different groups of either Rhodiola rosea L (2 × 200 mg dose Vitano®, 1 tablet taken before breakfast and 1tablet before lunch) or a control condition (no treatment). Self‐report measures and cognitive tests were completed at four testing sessions over a period of 14 days. Relative to the controls, the experimental group demonstrated a significant reduction in self‐reported, anxiety, stress, anger, confusion and depression at 14 days and a significant improvements in total mood. No relevant differences in cognitive performance between the groups were observed. Rhodiola rosea L (Vitano®) presented a favourable safety tolerability profile. Although this was a non‐placebo controlled trial, it is unlikely that the findings were the result of placebo effects as changes appeared gradual and were specific to certain psychological measures. However, we cannot determine a causal relationship; further investigations are recommended to support the effects of Rhodiola rosea L. extract on stress related symptoms. Copyright © 2015 John Wiley & Sons, Ltd.     

红景天根腐病的防治研究

对新疆红景天根腐病病因调查表明,低海拔区种植、7~8月高温、土壤湿度大(浇水多)亦是红景天根腐病流行的主要原因之一。中耕时适当扒土使红景天根茎部裸出地面和人工摘除花芽等措施可以减轻根腐病的危害。室内及田间药物防治试验表明:多菌灵、多菌灵 福美双混合剂600倍对红景天根腐病的防治效果显著,分别达到82.12%和85.34%。     

新疆蔷薇红景天黄酮类化学成分研究

目的:对新疆野生蔷薇红景天黄酮类化学成分进行研究。方法:利用硅胶柱色谱、SephadexLH-20柱色谱以及重结晶、制备液相等方法分离纯化,通过理化常数和波谱数据鉴定化合物结构。结果:从新疆蔷薇红景天中分离得到6个黄酮类成分,分别是草质素-7-O-α-L-鼠李糖苷(Ⅰ),草质素-3-O-β-D-葡萄糖-7-O-α-L-鼠李糖苷(Ⅱ),山柰酚(Ⅲ),山柰酚-7-O-α-L-鼠李糖苷(Ⅳ),山柰酚-3-O-β-D-葡萄糖苷(Ⅴ),山柰酚-3-O-β-D-(2″-O-β-D-吡喃木糖基)吡喃葡萄糖苷(Ⅵ)。结论:本研究为进一步有效合理开发利用新疆野生蔷薇红景天资源奠定了基础,为进一步的药理、药效学以及质量标准研究奠定了基础。     

红景天胶囊工艺的研究

目的选取最佳胶囊的制备工艺。方法红景天具有滋补强壮、抗缺氧、抗微波辐射、抗毒、活血化淤、抗肿瘤、抗衰老等作用,通过用正交实验选取最佳提取工艺,提取红景天中有效成分,制成便于服用的胶囊型制剂。结果各项指标的检测合格。结论此最佳工艺可用于经景天胶囊的制备。     

Rhodiola rosea extract protects human cortical neurons against glutamate and hydrogen peroxide-induced cell death through reduction in the accumulation of intracellular calcium

The aim of this study was to investigate the neuroprotective effects of a titolated extract from Rhodiola rosea L. (RrE) and of salidroside (Sa), one of the major biologically active compounds extracted from this medicinal plant, against oxidative stressor hydrogen peroxide (H₂O₂) and glutamate (GLU)‐induced cell apoptosis in a human cortical cell line (HCN 1‐A) maintained in culture. The results obtained indicate that exposure of differentiated HCN 1‐A neurons to GLU or H₂O₂ resulted in concentration‐dependent cell death. A 24 h pre‐treatment with RrE significantly increased cell survival and significantly prevented the plasma membrane damage and the morphological disruption caused by GLU or H₂O₂, indicating that neurons treated with RrE were protected from the neurotoxicity induced by the oxidative stressor used. In addition, RrE significantly reduced H₂O₂ or GLU‐induced elevation of intracellular free Ca²⁺ concentration. The results obtained have also shown that Sa caused similar effects in all experimental models used; however, the potency of the action was lower than that of the extract containing corresponding quantities of Sa. These findings indicate that RrE has a neuroprotective effect in cortical neurons and suggest that the antioxidant activity of the RrE, due to the structural features of the synergic active principles they contain, may be responsible for its ability to stabilize cellular Ca²⁺ homeostasis. Copyright © 2011 John Wiley & Sons, Ltd.     

Adaptogenic and central nervous system effects of single doses of 3% rosavin and 1% salidroside Rhodiola rosea L. extract in mice

Rhodiola rosea L., or 'golden root', is a popular plant in traditional medicine in Eastern Europe and Asia, with a reputation for improving depression, enhancing work performance, eliminating fatigue and treating symptoms of asthenia subsequent to intense physical and psychological stress. Due to these therapeutic properties, R. rosea is considered to be one of the most active adaptogenic drugs. To confirm and extend results obtained in the few preclinical and clinical studies available in English language journals, the purpose of the present study was to re-investigate the effects produced by a single oral administration of an R. rosea hydroalcohol extract (containing 3% rosavin and 1% salidroside) on the central nervous system in mice. The extract was tested on antidepressant, adaptogenic, anxiolytic, nociceptive and locomotor activities at doses of 10, 15 and 20 mg/kg, using predictive behavioural tests and animal models. The results show that this R. rosea extract significantly, but not dose-dependently, induced antidepressant-like, adaptogenic, anxiolytic-like and stimulating effects in mice. This study thus provides evidence of the efficacy of R. rosea extracts after a single administration, and confirms many preclinical and clinical studies indicating the adaptogenic and stimulating effects of such R. rosea extracts. Moreover, antidepressant-like and anxiolytic-like activities of R. rosea were shown in mice for the first time.     

大株红景天治疗不稳定型心绞痛患者的疗效及对hs-CRP、血脂、血液流变学指标的影响

目的研究大株红景天治疗不稳定型心绞痛的疗效及对患者血清hs-CRP、血脂、血液流变学指标的影响。方法选取2017年7月—2019年7月本院收治的不稳定型心绞痛患者82例作为研究对象。随机数字表法将患者分为观察组和对照组,每组41例。对照组给予常规干预治疗,观察组在常规干预治疗的基础上给予注射大株红景天注射液治疗。治疗持续四个疗程后,统计观察2组患者接受治疗后总有效率、症状改善情况、检测患者血清中超敏C-反应蛋白(hs-CRP),血脂水平;血液流变学指标。结果观察组治疗总有效率为87.80%显著高于对照组的56.10%(P<0.05);观察组患者每周发作频次、持续时长、视觉模拟评分法(VAS)疼痛评分情况显著优于对照组(P<0.05);观察组患者血清hs-CRP含量显著低于对照组;患者血脂(胆固醇、高密度脂蛋白、低密度脂蛋白、甘油三酯)改善情况显著优于对照组(P<0.05);患者血液流变学指标改善情况显著优于对照组(P<0.05)。结论通过在常规干预治疗的基础上联合注射红景天注射液治疗不稳定型心绞痛,可显著降低hs-CRP水平,改善血脂水平和血液流变学指标,提高治疗效果,值得临床推广。     

马齿苋甜菜红素抗肿瘤实验研究

目的 观察马齿苋甜菜红素联合环磷酰胺(CTX)的抗肿瘤作用.方法 将接种S_(180)瘤细胞的小鼠分为荷瘤对照组、CTX组、马齿苋甜菜红素(高、中、低剂量)+CTX组.给药10 d,进行抑瘤率计算,以及外周血常规、骨髓有核细胞计数、脾脏、胸腺指数、免疫球蛋白、肝肾功能等的检测.结果 与荷瘤对照组相比,CTX组肿瘤生长明显受到抑制.马齿苋甜菜红素低、中、高剂量联合CTX各组抑瘤率明显高于CTX组的抑瘤率.与荷瘤对照组相比,单一CTX处理组脾脏和胸腺指数、IgA、IgG、IgM水平明显降低,WBC、PLT和骨髓有核细胞计数降低,ALT、BUN升高.不同剂量的马齿苋甜菜红素联合CTX处理,可以不同程度地改善脾脏和胸腺指数、IgG、IgM等免疫水平指标,提高WBC、PLT和骨髓有核细胞的数量,降低ALT和BUN.结论 马齿苋甜菜红素对小鼠S_(180)肉瘤有抑制作用,对化疗药物CTX抗S_(180)小鼠具有增效减毒的作用.     

木芙蓉叶提取工艺的研究

目的：研究木芙蓉叶中总黄酮提取法的最佳工艺条件。方法：采用Ｌ９（３＾４）正交试验法,采用７０％乙醇回流法与水煎法、７５％乙醇温浸法比较。结果：乙醇回流提取法最好。结论：木芙蓉叶总黄酮提取的最佳工艺条件Ａ１Ｂ３Ｃ３Ｄ５。