胰岛素样生长因子Ⅰ的抗心肌细胞凋亡作用

研究了缺氧条件下引起培养的乳鼠心肌细胞凋亡及IGF－Ⅰ的抗凋亡作用。将原代培养的心肌细胞置于95%N     

血清胰岛素样生长因子在机体生长发育中的作用研究进展

胰岛素样生长因子(IGF-Ⅰ)是一类多功能细胞增殖调控因子,在细胞的分化、增殖、个体的生长发育中具有重要的促进作用.研究显示其在调节胎儿宫内生长发育、出生后婴幼儿的生长发育中有重要作用,是目前儿科临床研究的热点内容,本文对血清IGF-Ⅰ在胎儿及婴幼儿生长发育中的研究进展进行综述.     

宫内发育迟缓儿脐血胰岛素样生长因子1、胰岛素样生长因子结合蛋白3、胰岛素及瘦素的关系

目的探讨宫内发育迟缓(IUGR)儿出生后脐血胰岛素样生长因子-1(IGF-1)、胰岛素样生长因子结合蛋白3(IGFBP3)、胰岛素(Ins)及瘦素(Lep)的变化。方法选择141例IUGR儿为IUGR组,以同期出生的足月、适于胎龄儿30例作为对照组,比较两组出生后脐血IGF-1、IGFBP3、Ins和Lep的浓度。结果IUGR组IGF-1、IGFBP3、Ins和Lep的浓度均明显低于对照组(P值分别＜0．01、0．05)。IUGR组IGF-1与IGFBP3(r= 0．6434)和Ins(r=0．4367)、Ins与Lep(r=0．3461)均呈正相关(P值均＜0．01),IGF-1与Lep、IGFBP3与Ins和Lep均不相关(P值均＞0．05)。结论IGF-1、IGFBP3、Ins及Lep低下是导致IUGR的原因, Ins-IGF-1-IGFBP3及Ins-Lep轴各自调控胎儿的生长发育。     

胰岛素样生长因子在植入前胚胎细胞中的表达

目的 :研究胰岛素样生长因子 1,2 (IGF 1,IGF 2 )在植入前胚胎的表达及其生物学意义。方法 :采用原位杂交法 ,检测人植入前胚胎细胞IGF 1,IGF 2mRNA的表达情况。结果 :IGF 1及IGF 2mRNA于不同阶段的植入前胚胎细胞中表达不同 ,各表达量有差异。结论 :不同阶段的植入前胚胎细胞分泌IGF 1、IGF 2 ,参与早期人类胚胎的生长、发育。     

胰岛素样生长因子与胎儿宫内生长受限的相关性研究

目的探讨胰岛素样生长因子（IGF）与胎儿生长发育的关系。方法抽取20例分娩宫内生长受限（IUGR）胎儿（IUGR组）及25例分娩正常儿（对照组）产妇肘静脉血、新生儿脐静脉血及羊水和胎盘组织,采用放射免疫法及免疫放射法分别测定其IGF-Ⅰ、IGF-Ⅱ水平和RT-PCR法检测胎盘组织中mRNA含量。结果IU-GR组产妇血清IGF-Ⅰ水平低于对照组,IGF-Ⅱ水平差异无统计学意义;IUGR组脐血及羊水中IGF-Ⅰ、IGF-Ⅱ水平较对照组显著降低;IGF-Ⅰ、IGF-Ⅱ的mRNA水平较正常对照组低。结论检测母血及羊水中IGF-Ⅰ、IGF-Ⅱ水平可监测胎儿生长发育,早期诊断IUGR,IGF与IUGR的发病密切相关。     

FIGF-Ⅰ、IGFBP-1与糖尿病酮症关系的研究

目的观察FIGF-I、IGFBP-1与糖尿病酮症之间的关系.方法测定了60名1型糖尿病患者(新发病未治疗尿酮阳性及阴性者各15例,胰岛素治疗3个月至1年尿酮阳性及阴性者各15例)和15名健康对照者空腹血清FIGF-Ⅰ、IGFBP-1、游离胰岛素(Ins)、血糖、HbA1c、尿酮体.结果①1型糖尿病患者血清FIGF-I低于对照组(P<0.001),IGFBP-1高于对照组(P<0.001).血清FIGF-Ⅰ与IGFBP-1显著负相关(P<0.001),与Ins水平正相关(P<0.05);IGFBP-1与Ins负相关(P<0.001).②糖尿病酮症患者血清FIGF-Ⅰ低于非酮症组(P<0.01).结论 1型糖尿病患者血清FIGF-Ⅰ低于对照组,IGFBP-1高于对照组,酮症时尤为明显;降低的FIGF-Ⅰ可能参与患者体内酮体的生成.     

胰岛素样生长因子I受体在宫同内发育心缓胎鼠中的表达研究

目的 探讨宫内发育迟缓（IUGR）时胰岛素样生长因子I受体（IGFD－1R）表达的变化及其在组织和细胞中的定位。方法 将21只SD孕鼠随机分为实验组12只和对照组9只。钳夹实验组孕鼠双侧子宫动、静脉20分钟,建立宫内发育迟缓动物模型。对照组仅作开腹和关腹术。两组均于孕22天剖宫取出胎鼠,采用免疫组化法观察孕晚期胎鼠肝脏和肺组织中IGF－1R的表达。结果 实验组胎鼠的体重、身长及胎盘、肝、肺组织重量按对照组明显降低（P＜0．05）;实验组胎鼠肝脏IGF－1R表达面积比较对照组明显增加,平均灰度明显降低（P＜0．01）;实验组胎鼠肺组织IGF－1R表达面积也比较对照组明显增加（P＜0．05）,但平均灰度则无显著性差异（P＞0．05）。结论 宫内发育迟缓时IGF－1R在肝脏和肺组织的表达增加,可能是机体对IGF－1降低的代偿机制。     

胰岛素样生长因子Ⅰ及其受体在子宫内膜异位症中的表达

目的：探讨胰岛素样生长因子Ⅰ(IGF-Ⅰ)及其受体与子宫内膜异位症的关系。方法：应用免疫组织化学法（常规S-P法）对子宫内膜异位症患者的在位子宫内膜(17例)、异位子宫内膜(30例)进行IGF-Ⅰ及其受体的检测， 取25例正常子宫内膜组织作为对照。 结果：IGF-Ⅰ及其受体在3组子宫内膜的腺细胞强表达，IGF-Ⅰ在3组子宫内膜间质弱表达，IGF-Ⅰ受体在子宫内膜间质不表达。IGF-Ⅰ在子宫内膜异位症患者的在位子宫内膜、异位子宫内膜的表达均强于对照组正常子宫内膜（P<0.01）。IGF-Ⅰ受体在子宫内膜异位症患者的异位子宫内膜的表达强于其在位子宫内膜和对照组正常子宫内膜（P<0.01）。 结论：IGF-Ⅰ在子宫内膜异位症的发生、发展中起促进作用。     

Expression of insulin-like growth factor-1in bony class Ⅲ malocclusion

目的 研究在下颌骨发育过度导致的骨性Ⅲ类错(牙合)畸形患者中,胰岛素样生长因子-1(IGF-1)基因的表达,探讨IGF-1基因表达与下颌骨生长的关系.方法 选取骨性Ⅲ类错(牙合)畸形患者27例,骨性Ⅰ类患者(包括个别正常(牙合))27例,应用荧光定量PCR技术和酶联免疫吸附剂测定(ELISA)技术研究下颌骨中IGF-1基因的表达变化.结果 RT-PCR检测结果显示,骨性Ⅲ类错(牙合)畸形组中IGF-1mRNA的表达量为(5.5410±2.044 7)μg/μl,骨性Ⅰ类组中IGF-1mRNA的表达量为(1.2821±0.273 1)μg/μl,2组比较具有统计学差异(P＜0.05).根据1GF-1基因光密度值与浓度关系曲线,得回归系数0.998.ELISA检测结果显示,骨性Ⅲ类错(牙合)畸形组中IGF-1蛋白浓度为(84.1259±29.294 7) ng/L,骨性Ⅰ类组中IGF-1蛋白浓度为(22.406 4±4.9312) ng/L,2组相比具有显著差异(P＜0.05).结论 下颌骨生长量越大,IGF-1基因mRNA和蛋白表达越高.     

原发性高血压患者血清胰岛素样生长因子-1水平的变化

目的:探讨原发性高血压(EH)患者血清胰岛素样生长因子-1水平的变化规律.方法:采用放射免疫分析法(RIA)检测82例EH(Ⅰ级30、Ⅱ级30、Ⅲ级22)、20例经降压药物治疗血压恢复至正常水平患者和30名健康人血清胰岛素样生长因子-1(IGF-1)水平,并与高血压临床分级进行相关分析.结果:血清IGF-1水平,EH组[(142.9±37.9)ng/ml]明显高于对照组[(93.6±25.4)ng/ml],差异有显著性(P＜0.01),并且与高血压临床分级相关(γ=0.422,p＜0.05);治疗后IGF-1水平(110.7±34.9 ng/ml)明显低于治疗前[(155.8±38.2)ng/ml](P＜0.01).结论:EH患者血清IGF-1明显升高,可能参与了EH发生和发展的病理、生理过程.     

Association of Insulin-like Growth Factors with Lung Development in Neonatal Rats

To explore the relationship between Insulin-like growth factor (IGF)-Ⅰ,-Ⅱ and lung development in neonatal rats. 80 timed pregnant Sprague-Dawley (SD) rats were randomly divided into 4 groups (n=20): group A (Control group), group B (Dexamethasone (DEX) 1 group),group C (DEX 2 group), group D (retinoic acid (RA) group). 20 pregnant rats in group A, B and D were injected subcutaneously or intraperitoneally with vehicle (NS), DEX, or RA respectively during gestational day 16 to 18. All newborn rats in group C were subcutaneously injected with DEX at day 1 to 3 after birth. The lung tissue was obtained at the following times: fetuses at gesta-tional ages of 18, 20 and 21 days, and 1, 3, 5, 7, 10, 14 and 21 days after birth. Lung tissues were used for histopathological study, the polypeptides analysis of IGF-Ⅰ, -Ⅱ(immunohistochem-istry and Western blot) and mRNA analysis ( RT- PCR). The results showed that the strongest expression of IGF-Ⅰ in group A and D occurred at ages of 5—7 days (alveolar stage). The stronger their expressions, the better the alveolar develop. The peak stage of expression in group B occurred earlier, on the day 3 after birth. Compared with group A, the expression of IGF-Ⅰ during gestation age of 18 days to age of 3 days in group B were significantly higher (P＜0.01), but significantly lower at other time points (P＜0.01). The expression of IGF-Ⅰ was lower in group C all the time and always higher in group D than those in group A (P＜0.01). The peak expression of IGF-Ⅱ took place at the gestation age of 18 days, then gradually dropped to trace. During 18 days of gestation to age of 3 days, the expression of IGF-Ⅱ in group B was significantly higher than that in group A (P＜0.01). No difference was found among all other groups. The change in the expression of IGF-Ⅰ, -Ⅱ mRNA in all 4 groups was similar to that of their polypeptides. The results suggested that there is a close linking between IGF- Ⅰ, -Ⅱ and lung development in newborns. The IGF-Ⅱ works at early stage and the that of IGF-Ⅰ works at the stage of new septa formation and alveoli maturation. The stronger their expressions, the more mature the lung development.     

胰岛素样生长因子结合蛋白在人类恶性肿瘤中的研究进展

胰岛素样生长因子(insulin—like growth factor，IGF)系统由IGF及其受体和胰岛素样生长因子结合蛋白(insulin-like growth factor binding protein，IGFBP)组成，体内外研究表明，它们调控着细胞的生长和增殖。IGF是一种强力的促细胞分裂素，其作用决定于IGF与其受体的相互作用。IGFBP是由6种与IGF高亲和力结合蛋白组成的家族，不仅具有运载蛋白的功能，而且通过IGF依赖性或非依赖性机制抑制或促进IGF的作用。近年的研究表明，IGFBP与肿瘤的发生、发展密切相关。     

The change of insulin-like growth factor-1 in diabetic patientswith neuropathy

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胰岛素样生长因子-1受体在植入前胚胎细胞中的表达

目的 :研究胰岛素样生长因子 1受体 (IGF 1R)在植入前胚胎的表达及其生物学意义。方法 :采用免疫组化ABC法 ,检测人植入前胚胎细胞IGF 1R的表达情况。结果 :不同阶段的植入前胚胎细胞均有IGF 1R表达 ,各表达程度无明显差异。结论 :IGF 1R在植入前胚胎中均有表达 ,提示IGF 1R可结合IGF 2、IGF 1,参与人植入前胚胎的生长、发育和着床。     

胰岛素样生长因子与肝纤维化的研究进展

肝星状细胞(hepatic stellate cells,HSCs)的激活和增殖是肝纤维化的中心环节,胰岛素样生长因子(insulin-like growth factors,IGFs)是一类重要的促细胞活化、分裂的生长因子.IGFs与其受体结合后通过Ras/细胞外信号调节激酶途径、PI3-K途径、影响Na /H 交换以及抑制肝星状细胞凋亡等方式促进肝星状细胞增殖,从而加速肝纤维化.     

胰岛素样生长因子对早期胚胎发育的保护作用

目的:探讨胰岛素样生长因子-I和II(IGF-I和-II)在正常胚胎发育及早期胚胎发育终止绒毛及蜕膜组织中的表达及其在母体血中水平的改变与胚胎发育的关系. 方法:分别采用免疫组织化学SP法和原位杂交法检测正常胚胎发育绒毛及早期胚胎发育终止绒毛中IGF-I和蜕膜中IGF-II蛋白的表达以及IGF-I mRNA和蜕膜中IGF-II mRNA的表达;采用酶联免疫吸附试验(ELISA)检测正常妊娠及早期胚胎发育终止孕妇血中IGF-I和II水平的变化. 结果:免疫组化结果显示,正常妊娠绒毛中IGF-I和蜕膜中IGF-II的表达明显高于胚胎发育终止组(P＜0.01);原位杂交结果显示,正常妊娠组绒毛中IGF-I和蜕膜中IGF-II mRNA的表达明显高于胚胎发育终止组(P＜0.01);正常妊娠组母血中IGF-I和II水平显著高于胚胎发育终止组(P＜0.01). 结论:IGF-I和II在绒毛组织中的表达及母血中的水平的升高对早期胚胎发育有重要的保护作用,其含量的减少可能是导致胚胎死亡的重要原因之一. IGF-I和II可能成为预测胎儿安危的指标之一.     

Levels of insulin-like growth factor after stem cell transplantation

Some cytokines, i.e. tumor necrosis factor-, interleukin-6 and soluble interleukin-2 receptors are associated with complications of stem cell transplantation. Insulin-like growth factors (IGFs) are a family of peptides essential for the proliferation of normal and malignant cells. Recently increased levels of IGFs have been associated with the development of malignant tumors. In this communication we report on 96 measurements of insulin-like growth factor-I (IGF-1), insulin-like growth factor-II (IGF-2), and insulin-like growth factor-binding protein-3 (IGFBP-3) performed in 19 patients following stem cell transplants. Seventeen patients had allogeneic and 2 patients autologous transplants. Most IGF determinations were made at days 0, 7, 14, 21 and 28, some at other time points. The baseline values (day 0) of IGF-1 and IGFBP-3 were not different from controls. IGF-2 values were slightly lower than controls. Following transplantation, a consistent increase of IGF-1 was observed in 9/16 patients at days 7 and 14. Later the values decreased again. IGF-2 and IGFBP-3 did not change significantly after transplantation. No direct correlation could be established with the severity of graft-versus-host disease, levels of interleukin-6 and the time to hematopoietic recovery. A potential relevance of IGFs following stem cell transplantation may be the early diagnosis of liver damage and the development of second malignancies. More studies are necessary to investigate the pathophysiology and the clinical relevance of the increase of IGF-1 following stem cell transplantation.     

胰岛素样生长因子-1与骨重建

胰岛素样生长因子-1 (insulin-like growth factor-1,IGF-1)是1种由70个氨基酸组成的单链多肽,几乎所有组织中都含IGF-1,现将IGF-1的研究现状作一综述.1 IGF-1的发现和命名Salmon和Daughaday首次发现IGF-1能促进小鼠软骨组织对硫酸盐35S的吸收,当时命名为"硫酸化因子".1963年,Froesch等将它描述为具有不可抑制的胰岛素样活性物质.1972年,又将它命名为"促生长因子".1976年Rinderknecht和Humbel从人血浆中分离出活性物质,由于它的结构与胰岛素原相似,重新将它命名为胰岛素样生长因子-1[1].     

脐静脉血清IGF-2水平与巨大儿发生的关系

目的：探讨脐静脉血清胰岛素样生长因子-2（IGF-2）水平与巨大儿（MAC）的关系。方法：选择2018 年1月至2019 年12月出生于温州医科大学附属第一医院的足月MAC和正常出生体质量儿（NBW）分别61例和60 例。检测脐静脉血血清IGF-2 水平,并采用Pearson或Spearman相关分析法分析其与新生儿生长发 育指标的相关性。结果：MAC和NBW组的脐静脉血清IGF-2水平分别为（221.92±29.97）ng/mL和（169.69±14.53）ng/mL,2组间差异有统计学意义（P ＜0.001）;在MAC和NBW组中,母亲合并妊娠期糖尿病（GDM）新生儿的脐静脉血清IGF-2水平高于母亲无GDM者（GDM-MAC组 vs. nGDM-MAC组为229.71 ng/mL vs. 214.18 ng/mL,P =0.046;GDM-NBW组 vs. nGDM-NBW组为175.69 ng/mL vs. 161.48 ng/mL,P ＜0.001）。相关性分析显示脐静 脉血清IGF-2水平与新生儿出生体质量、身长和重量指数呈正相关（r =0.678、0.566、0.600,均P ＜0.001）。IGF-2水平也与胎龄相关（r =0.273,P =0.002）,但与新生儿性别、母亲年龄、高学历和初产无关（P ＞0.05）。结论：新生儿脐静脉血清IGF-2水平受母亲GDM和胎龄影响;IGF-2可反映胎儿生长发育状况,高水平IGF-2与巨大儿发生有关。