斑点ELISA法检测流行性出血热患者血清中特异性抗体

<正> 检测血清中流行性出血热(EHF)特异性抗体大都用间接免疫荧光法(IFAT),但该法由于需要荧光显微镜等特殊设备,因而在基层的推广使用常受到限制。为此,本实验将斑点ELISA(DIA)技术应用于病人血清特异性抗体的检测,同时与IFAT作一比较,获得满意结果,现报告如下。     

流行性出血热（EHF）病毒间接ELISA抗原制备的研究

本文报告用硫酸鱼精蛋白结合高浓度盐溶液方法从感染鼠脑组织中提取用于EHF间接ELISA的病毒抗原,对抗原提取条件进行了研究,并成功地将提取的病毒抗原用于检测HFRS病人血清特异性IgG抗体的间接ELISA。     

应用Hep-z细胞从褐家鼠肺中分离出流行性出血热病毒

<正> 1976年,Lee氏应用非疫区的黑线姬鼠从疫区黑线姬鼠肺中分离出流行性出血热病毒(EHFV)。此后,应用A549(人肺癌)。Veto-E_6(绿猴肾)、2BS(人胚肺二倍体)等细胞分离EHFV相继获得成功。但应用Hep-2细胞(人喉癌细胞)从鼠肺标本中分离出EHFV尚未见报道。我们于1991年至1992年首次应用Hep-2细胞从闽南的褐家鼠肺中分离出EHFV,现报道如下。     

猪在流行性出血热传播中的作用──Ⅱ、养猪户人群中流行性出血热病毒抗体的检测

猪在流行性出血热传播中的作用──Ⅱ、养猪户人群中流行性出血热病毒抗体的检测江西医学院微生物学教研室陈瑞琪,刘佩芹,谌济国,余克花江西省上高县卫生防疫站龙健自１９８８年张云等［１］首次从猪肺中分离到流行性出血热（ＥＨＦ）病毒,关于猪在ＥＨＦ传播中的作用...     

双重免疫荧光抗体法在流行性出血热抗体检测中的应用

双重免疫荧光抗体法在流行性出血热抗体检测中的应用浙江省上虞县卫生防疫站贾杰流行性出血热（ＥＨＦ）血清抗体的检测，对ＥＨＦ的诊断十分重要。近年用于检测的方法较多［１～５］，但多数使用免疫荧光法（ＩＦＡＴ）。本文在常规荧光法的基础上，加用第二荧光抗体（第...     

猪脾细胞干扰素抗流行性出血热病毒的初步研究

根文首次报道猪脾细胞干扰素在细胞培养中对流行性出血热病毒具有抑制作用,使感染细胞内病毒抗原的表达减少,并对细胞无毒性反应。此外,猪脾细胞干扰素和病毒同时作用细胞,或先感染病毒2小时后,再加猪脾细胞干扰素对病毒的抑制作用要大于先用猪脾细胞干扰素处理6小时后再感染病毒。此结果为猪脾细胞干扰素可用于治疗流行性出血热提供了依据。     

流行性出血热患者特异性抗体的比较研究

流行性出血热患者特异性抗体的比较研究刘正稳，张成文，樊万虎为研究流行性出血热（ＥＨＦ）患者病程中特异性ＩｇＧ抗体、中和抗体（ＮＡｂ），血凝抑制抗体（ＨＩＡｂ）之间的相互关系，及探讨各种特异性抗体在发病机制及病情转旧中的作用，我们对１０例ＥＨＦ患者８２...     

Modulation of acetylcholine receptor by antibody against the receptor

Antibody against acetylcholine receptor induces an increase in the rate of degradation of acetylcholine receptors on a mouse cell line (BC(3)H-1) and cultured rat skeletal muscle. The increased rate of degradation results in a lowered density of acetylcholine receptors on muscle membrane and a lowered sensitivity to iontophoretically applied acetylcholine. The modulation of acetylcholine receptor is energy, temperature, and time dependent and may be related to antigenic modulation found in other systems. Acetylcholine noise analysis demonstrates that antibody against acetylcholine receptor reduces the channel mean conductance and mean open time slightly.It is concluded that antibody binds to the acetylcholine receptor, impairs its function, and induces receptor degradation. This results in a lowered density of acetylcholine receptor and a lowered sensitivity to acetylcholine. Patients with myasthenia gravis have antibodies to their acetylcholine receptor in their serum. Antigenic modulation of receptor in the muscle of patients with myasthenia gravis could contribute to the observed decrease in amplitudes of miniature endplate potentials and in muscle acetylcholine sensitivity, and the symptoms of muscular weakness.     

Protection against bacteremic pneumococcal infection by antibody to pneumolysin

Pneumolysin is an important virulence factor of Streptococcus pneumoniae. This study examined the hypothesis that human antibody to pneumolysin provides protection against pneumococcal infection. At the time of hospital admission, patients with nonbacteremic pneumococcal pneumonia had higher levels of serum anti-pneumolysin IgG than did patients with bacteremic pneumococcal pneumonia or uninfected control subjects. IgG levels rose significantly during convalescence in patients with bacteremic pneumonia, reaching levels observed in nonbacteremic patients. Purified human anti-pneumolysin IgG protected mice against intraperitoneal challenge with S. pneumoniae types 1 or 4 in a dose-related fashion; mice that received anti-pneumolysin IgG had a greater likelihood of surviving challenge and had negative blood cultures. Pneumolysin damages epithelial cells and inhibits phagocytic function of polymorphonuclear leukocytes. One hypothesis that might explain the study results is that, early in infection, IgG to pneumolysin blocks these effects in the alveoli, thereby protecting the host against bacteremic pneumococcal disease.     

Monoclonal antibody against human renin

By spleen cell fusion with NS1 myeloma, a mouse hybridoma was obtained which secretes an antibody directed against human renin. This monoclonal antibody recognizes human and monkey renin, but neither hog nor mouse. Preliminary experiments demonstrate the potential of this antibody for renin immunopurification and characterization.     

Antibody-dependent cell-mediated cytotoxicity against cell lines generated by liver-specific idiotype-bearing antibody

We produced a murine monoclonal antibody (mAb), designated H2-mAb, against a fractionated soluble phase of human liver homogenate which antibody reacted with human liver cells. A human antibody possessing the same idiotype as the H2-mAb, designated LSIA (liver-specific idiotype-bearing antibody), can be measured by a sandwich enzyme-linked immunosorbent assay, using the anti-H2 idiotype antibody. The serum level of LSIA in patients with histologically proven chronic hepatitis (CH) was significantly higher than that in healthy subjects and it was also higher than that in subjects with other diseases, including systemic lupus erythematosus. In a comparison between patients with CH type B and those with CH type C, there was no significant difference in serum levels of LSIA. It was possible to purify LSIA from the sera of patients with CH. The purified LSIA bound to the human cell lines Chang and HCC-M, derived from liver cells and a hepatoma respectively, but not to HeLa cells, a uterine carcinoma derivative. The reactivity of this mAb to HCC-M was weaker than that to Change. Moreover, the presence of LSIA caused an antibody-dependent cell-mediated cytotoxic challenge against Change cells in vitro.     

流行性出血热病毒血凝和血凝抑制试验的改进

本文对流行性出血热研究和诊断中传统采用的血凝和血凝抑制试验进行较大改进。比较7种制备流行性出血热病毒血凝素的方法,首次推出制备较高滴度血凝素的吐温80-乙醚法;试用含CaCl_2和MRSO_4 各0.05％的pH9.0 BABS稀释血凝素,血凝滴度较仅用pH9.0 BABS稀释者高4 倍;测定6种动物血球对血凝素的敏感性,找到了比鹅血球更敏感的鸽血球;采用以二甲亚砜为主要成份的血球冻存液,将鸽血球保存于-20℃,大大延长了血球使用期限而不影响其质量。