乳腺癌组织中上皮钙黏蛋白和B细胞淋巴瘤基因2的表达及其临床意义的研究

目的:探讨乳腺癌中上皮钙黏蛋白（E-cadherin）和B细胞淋巴瘤基因2（Bcl-2）的表达及其临床意义。方法:使用免疫组织化学方法检测68例乳腺癌旁正常组织、41例纤维腺瘤组织和74例乳腺癌组织的E-cadherin和Bcl-2的表达水平,并分析乳腺癌患者各病理特征与E-cadherin和Bcl-2表达的相关性。结果:乳腺癌旁正常组织和纤维腺瘤组织中E-cadherin阳性率高于乳腺癌组织,Bcl-2阳性率低于乳腺癌组织,差异具有统计学意义（P均＜0.05）。乳腺癌组织病理分级越高、TNM分期越严重、有淋巴结转移和复发的患者 E-cadherin阳性率低,Bcl-2阳性率高;ER阳性患者E-cadherin阳性率高,Bcl-2阳性率高。组织病理分级低-中分化患者E-cadherin阳性率高于高分化患者,Bcl-2阳性率低于高分化患者,差异具有统计学意义（P均＜0.05）;TNM分期Ⅰ+Ⅱ期患者E-cadherin阳性率高于Ⅲ期,Bcl-2阳性率低于Ⅲ期,差异具有统计学意义（P均＜0.05）;有淋巴结转移患者E-cadherin阳性率低于无转移者,Bcl-2阳性率高于无转移者,差异具有统计学意义（P均＜0.05）;雌激素受体（ER）阴性患者E-cadherin、Bcl-2阳性率低于ER阳性者,差异具有统计学意义（P均＜0.05）;复发患者E-cadherin阳性率低于无复发者,Bcl-2阳性率高于无复发者,差异具有统计学意义（P均＜0.05）;Bcl-2的表达与E-cadherin的表达存在负相关性（r=-0.638,P＜0.05）。结论:乳腺癌组织中E-cadherin表达降低,Bcl-2表达升高,二者与乳腺癌组织病理分级、TNM分期、淋巴结转移、ER和复发有关,E-cadherin和Bcl-2的表达存在相关性。     

粘附分子E-cadherin在乳腺肿瘤中的异常表达及其意义

E—cadherin是介导上皮细胞间粘附的一种粘附分子,其功能异常与肿瘤的浸润和转移密切相关。乳腺肿瘤中,E—cadherin表达缺失或下降,在肿瘤的浸润转移过程中起着非常重要的作用。基因突变、基因启动子区甲基化或转录抑制等是E—cadherin表达异常的原因。     

上皮型钙黏蛋白在宫颈癌中的表达及其临床意义

目的 探讨上皮型钙黏蛋白（Ｅ－ｃａｄｈｅｒｉｎ,Ｅ－ＣＤ）在宫颈癌前病变及宫颈癌中的表达及其临床意义。方法 应用Ｓ－Ｐ免疫组化法和抗Ｅ－ＣＤ抗体观察６０例宫颈癌、１０例正常宫颈上皮组织和１２例宫颈上皮内瘤样病变（ＣＩＮ）中Ｅ－ＣＤ的表达。结果 正常宫颈上皮组织中Ｅ－ＣＤ１００％表达,ＣＩＮ中Ｅ－ＣＤ阳性率为６６．７％,而宫颈癌组织中Ｅ－ＣＤ阳性率仅为４６．７％。随着肿瘤病理分级的升高,Ｅ－ＣＤ表达下降,其Ⅰ、Ⅱ、Ⅲ级Ｅ－ＣＤ阳性率分别为７１．４％、４６．２％、和３０．０％（Ｐ〈０．０５）。Ｅ－ＣＤ表达在淋巴结转移组（２７．３％）明显低于无淋巴结转移组（７５．９％,Ｐ〈０．０５）。临床分期愈晚,Ｅ－ＣＤ表达愈低,Ⅰ～Ⅱ期和Ⅲ～Ⅳ期Ｅ－ＣＤ阳性率分别为６２．５％和１５．０％（Ｐ〈０．０５）。结论 随着宫颈癌的发生发     

The expression and clinical significance of the androgen receptor and E-cadherin in triple-negative breast cancer

Triple-negative breast cancer (TNBC) has a poor prognosis and lacks prognostic indicators. The androgen receptor (AR) and E-cadherin are involved in the pathogenesis of breast cancer, but their roles are not clearly defined. We designed this study to evaluate AR and E-cadherin expression and to determine their relationships with the clinicopathologic parameters of triple-negative breast cancer. The present study included 127 TNBC patients. Immunohistochemical stains for AR and E-cadherin were performed, and the relationships between AR and E-cadherin expression and clinicopathologic data and prognosis were analyzed. We found that in TNBC patients, AR was expressed in 16(12.6%) cases, and E-cadherin was expressed in 41(33.0%) cases. AR expression was associated with tumor grade (P = 0.004) and menopausal status (P = 0.017), and E-cadherin expression was associated with node status (P= 0.016). A multivariate analysis demonstrated that tumor size, tumor grade, lymph node status, and E-cadherin were of prognostic significance for disease-free interval and overall survival. Compared with AR-positive patients, AR-negative patients showed significantly poorer outcomes with respect to the disease-free interval (P = 0.047) and overall survival (P = 0.038). E-cadherin-negative patients experienced shorter disease-free interval (P = 0.016) and poorer overall survival (P = 0.012) than did E-cadherin-positive patients. An AR-positive and E-cadherin-negative expression profile was associated with recurrence or metastasis (P = 0.036). Moreover, as the expression of nuclear AR increased (25% vs. 33.3%, P = 0.361), less E-cadherin staining was observed in TNBC samples. This finding suggested that AR and E-cadherin expression could be a useful prognostic marker for classifying subgroups of TNBC.     

Vimentin和E-cadherin蛋白在乳腺癌组织中的表达及其临床意义

目的: 研究波形蛋白（Vimentin）和E-钙黏蛋白（E-cadherin）在人乳腺癌组织中的表达及其临床意义。方法: 回顾性分析2014 年1 月至2016 年1 月在安徽医科大学附属巢湖医院接受乳腺癌根治术的56 例乳腺癌组织和相应癌旁组织标本以及病历资料,用免疫组织化学染色法和qPCR 分别检测癌组织中Vimentin、E-cadherin 蛋白和mRNA的表达,分析Vimentin 和E-cadherin蛋白在乳腺癌组织中的表达与临床病理特征的关系。用Logistic 多因素回归分析影响Vimentin 和E-cadherin 蛋白表达的独立因素,用Spearman 分析Vimentin 与E-cadherin 蛋白表达的相关性,用Kaplan-Meier 分析Vimentin 和E-cadherin 蛋白表达与预后的关系,用ROC曲线分析Vimentin 和E-cadherin 蛋白表达对预后的诊断。结果: 56 例乳腺癌组织中Vimentin 和E-cadherin 蛋白高表达率分别为76.79%和19.64%;其中47 例（47/56,83.93%）乳腺癌组织中Vimentin mRNA 的表达量显著高于癌旁组织（P<0.05）,46 例（46/56,82.14%）乳腺癌组织中E-cadherin mRNA的表达量显著低于癌旁组织（P<0.05）。Vimentin 蛋白表达与肿瘤大小、淋巴结转移、脉管侵袭、组织学分级、临床分期、分子分型、Ki67 阳性、ER阴性、PR阴性及HER2 阴性表达均有关（均P<0.05）;E-cadherin 蛋白表达与淋巴结转移、脉管侵袭、组织学分级、临床分期、分子分型、Ki67 阳性、ER阴性、PR阴性及HER2 阴性表达均有关（均P<0.05）。肿瘤大小、淋巴结转移、脉管侵袭、组织学分级、临床分期、分子分型、Ki67 阳性、ER阴性、PR阴性及HER2 阴性表达均是促进Vimentin 和E-cadherin 表达的独立影响因素（P<0.05）,且Vimentin 与E-cadherin 蛋白表达呈负相关关系（P<0.05）。Vimentin 蛋白高表达的患者3 年生存率为67.44%,E-cadherin 蛋白低表达的患者3 年生存率为68.89%。结论: 在乳腺癌组织中Vimentin高表达和E-cadherin低表达与乳腺癌发生发展、侵袭转移及患者预后有关,可作为临床诊断与预后的评价指标。     

E-cadherin在不同分子分型乳腺癌中的表达及其在三阴性乳腺癌中的临床意义

目的:探讨不同分子分型乳腺癌中E－钙黏素蛋白（E-cadherin）的表达及其在三阴性组中的临床意义。方法:收集2012年1月至2013年12月乳腺浸润性导管癌共188例,应用免疫组织化学及荧光原位杂交技术将乳腺癌分为Luminal A型49例、Luminal B型41例、HER-2阳性型44例,三阴性组（TNBC）54例。免疫组织化学检测的E-cadherin的表达情况同时分析E-cadherin在TNBC中与临床病理特征关系,并随访54例TNBC患者3年复发或转移率及PFS。结果:乳腺癌中E-cadherin阳性率为52.13%,Luminal A型组阳性率为63.27%、Luminal B型组阳性率为63.41%、HER-2阳性组阳性率为43.18%及TNBC组为42.59%,四组间阳性率差别具有统计学意义(χ2=7.89,P<0.05)。TNBC与HER-2阳性组间差别无统计学意(χ2=0.003,P>0.05),而TNBC组阳性率较Luminal A型组和Luminal B 型组均明显降低,差别具有统计学意义(42.59% vs 63.27%,χ2=4.40,P<0.05,42.59% vs 63.41%,χ2=4.05,P<0.05);TNBC中E-cadherin阳性表达与年龄无关 (P>0.05),与肿瘤大小、TNM分期、组织学分级、淋巴结转移状态及Ki67负相关,差别具有统计学意义(P<0.05);TNBC总体3年复发或转移率高达55.56%,无病生存率为44.44%。E-cadherin阳性组复发转移率为39.13%,E-cadherin阴性组复发转移率为67.74%,差别有统计学意义（χ2=4.38,P<0.05）,3年无疾病进展时间（PFS）E-cadherin阳性组明显优于阴性组（P<0.05）。结论:不同分子分型乳腺癌中E-cadherin表达不同,E-cadherin阳性表达与肿瘤大小、组织学分级、分期及浸润转移呈负相关,对预测3年复发转移有一定意义。     

Altered expression of E-cadherin in gastric cancer tissues and carcinomatous fluid.

Expression of E-cadherin in 21 patients with various histological types of gastric carcinomas was studied by immunoperoxidase staining. Intercellular boundaries of almost all cancer cells in well and moderately differentiated adenocarcinomas stained as deeply for E-cadherin as normal gastric mucosa. However, singly infiltrating cells of those histological types were poorly stained. In poorly differentiated adenocarcinomas, cancer cells forming clusters stained lightly and those infiltrating singly stained even less. In signet ring cell carcinomas, hardly any staining was observed. In each histological type, the staining patterns and intensity at different layers of the gastric wall, were essentially the same. Cancer cells from carcinomatous ascites of gastric adenocarcinomas and pancreatic adenocarcinomas, and those from pleural effusion of lung adenocarcinomas were also studied by immunofluorescence staining. Of 11 specimens, ten were negative and only one from a lung adenocarcinomas was positively stained. By phase-contrast microscopic observations, none of these cancer cells including those from the lung adenocarcinomas, formed obvious cell-cell contacts. Cell aggregation assays confirmed the above results. The molecular weight of E-cadherin of cancer cells of lung adenocarcinomas was less than intact E-cadherin as revealed by Western blot analysis. These results suggest that depressed expression and/or impaired function of E-cadherin in cancer cells, facilitates their liberation from primary sites to infiltrate freely into tissue or fluid.     

E-钙黏附素及α-链接素的表达与胃癌生物学行为及淋巴结转移规律的关系

目的 探讨上皮细胞黏附分子E－钙黏附素（E－CD）及链接素－α（α-CA）在胃癌的表达及其与胃癌生物学行为的关系。方法 采用免疫组化技术（ABC法）对70例手术切除新鲜胃癌组织进行了E－CD及α-CA表达情况的检测,每例均行腹腔脱落癌细胞和系统病理学检查。结果 胃癌组织E－CD及α-CA表达阳性率分别为38．6％和28．6％。在侵袭型、未分化型、弥漫状生长、侵透浆膜（T3／T4）、淋巴结转移阳性及Ⅲ、Ⅳ期胃癌中,E－CD及α-CA表达显著减弱（P＜0．025－0．005）。在淋巴结多数转移（＞5个）,转移度＞50％及转移至Ⅱ站以远的胃癌中,E－CD及α-CA表达亦明显减弱,且α-CA比E－CD降低更明显（P＜0．05－0．005）。E－CD（＋）α-CA（-）及E－CD（－）／α-CA（-）组淋巴结转移率和脱落癌细胞阳性率均高于E－CD（＋）／α-CA( )组（P＜0．05－0．005）,但E－CD（＋）／α-CA（-）联合检测对评估胃癌侵袭深度、淋巴结转移程度、肝转移、TNM分期及腹腔癌细胞脱落状态有较大意义,其中α-CA比E－CD表达更敏感。     

E-钙黏蛋白及Ki67在三阴性乳腺癌中的表达及临床意义

目的探讨E-钙黏蛋白、Ki67在三阴性乳腺癌(TNBC)中的表达及临床意义。 方法回顾性分析陆军军医大学新桥医院2010年1月至2013年12月收治的77例女性TNBC患者临床资料。采用免疫组织化学方法检测E-钙黏蛋白和Ki67的表达情况,通过χ²检验分析其与患者临床病理资料的关系。由于77例患者中有5例失访,本研究仅对随访资料完整的72例患者采用Kaplan-Meier法、Log-rank检验、Cox逐步回归模型进行生存分析和危险因素分析。 结果在77例患者中,E-钙黏蛋白的表达与淋巴结状态有关(χ²＝16.428,P<0.001),而Ki67表达与组织学分级有关(χ²＝7.218,P＝0.007)。中位随访59个月,72例患者的DFS率和OS率分别为58.3%、68.1%。其中,E-钙黏蛋白高表达者DFS率和OS率均高于低表达者(DFS率：75.9%比46.5%,χ²＝7.553,P＝0.006;OS率：82.8%比58.1%,χ²＝5.132,P＝0.023),而Ki67低表达者DFS率和OS率均高于高表达者(DFS率：84.0%比44.7%,χ²＝9.486,P＝0.002;OS率：92.0%比55.3%,χ²＝9.006,P＝0.003)。Cox逐步回归模型分析显示,淋巴结转移、组织学分级高是患者DFS的独立危险因素(OR＝4.030, 95%CI：1.854～8.757, P<0.001; OR＝2.879,95%CI：1.359～6.100,P＝0.006),Ki67高表达、淋巴结转移是OS的独立危险因素(OR＝5.067,95%CI：1.179～21.768, P＝0.029; OR＝6.253,95%CI：2.296～17.034, P<0.001)。 结论E-钙黏蛋白高表达和Ki67低表达的TNBC预后良好,这对于乳腺癌的个体化治疗具有一定的指导意义。     

E-钙粘附素在胃癌表达的临床意义

目的：探讨Ｅ－钙粘附素（Ｅ－ＣＤ）表达与胃癌发生发展及预后的关系。方法：采用ＳＰ免疫组化染色技术,检测２０例正常胃粘膜上皮,４３例异型增生和１０１例胃癌组织Ｅ－ＣＤ的表达情况。结果：正常胃粘膜和异型增生组织Ｅ－ＣＤ呈强阳性表达,８８．１％（８９／１０１）胃癌Ｅ－ＣＤ表达减弱,Ｅ－ＣＤ表达与胃癌分化差、浸润型生长显著相关（Ｐ〈０．０５）,Ｅ－ＣＤ表达正常的胃肿病人术后３、５年自下而上率显著高于减弱者     

Clinical significance of E-cadherin and vimentin co-expression in breast cancer

We examined the clinical significance of E-cadherin and vimentin expression in 83 patients with breast dancer. E-cadherin expression was correlated with axillary metastases, histologic grade, recurrence and disease-free survival, but not with overall survival. On the other hand, vimentin expression was correlated with axillary metastases and histologic grade, but not with recurrence, disease-free or overall survival. However, the co-expression of E-cadherin and vimentin was associated with axillary metastases, histologic grade and recurrence, and appeared as the most important prognostic factor in disease-free and overall survival. We therefore concluded that E-cadherin and vimentin expression in combination might provide prognostic information for breast cancer patients.     

Significance of E-cadherin expression in triple-negative breast cancer

Purpose:

Triple-negative breast cancer (TNBC), a subtype of breast cancer that is oestrogen receptor (ER) negative, progesterone receptor (PR) negative, and human epidermal growth factor receptor 2 (HER2) negative, has a poor prognosis. Although a correlation between E-cadherin expression level and outcome has been demonstrated among all types of breast cancer, little is known about the significance of E-cadherin expression levels in TNBC.

Methods:

A total of 574 patients who had undergone a resection of a primary breast cancer except for invasive lobular carcinomas were enrolled in this study. Expressions of ER, PR, HER2, and E-cadherin were assessed by immunohistochemistry. We examined the association between TNBC and other clinicopathological variables and evaluated the significance of the E-cadherin expression.

Results:

Among the 574 breast cancer cases, 123 (21.4%) revealed a triple-negative phenotype. Patients with TNBC experienced more frequent lymph node metastasis (P=0.024) and a poorer prognosis (P<0.001) in comparison with non-TNBC patients. Triple-negative breast cancer was an independent prognostic factor. Reduced levels of E-cadherin were observed in 238 (41.5%) of the 574 breast cancer cases. E-cadherin reduction was significantly frequent in cases of TNBC (P<0.001) and lymph node metastasis (P=0.032). Furthermore, in the 123 TNBC cases, the prognosis of patients with an E-cadherin-negative expression was significantly worse than that of E-cadherin-positive patients (P=0.0265), especially for those in clinical stage II (P=0.002). A multivariate logistic regression analysis showed a reduction of the E-cadherin expression to be an independent prognostic factor (P=0.046).

Conclusion:

E-cadherin expression may be a useful prognostic marker for classifying subgroups of TNBC.     

人类乳腺癌组织中骨膜素的表达及其意义

目的研究乳腺癌组织中骨膜素(periostin,PN)的表达情况。方法应用RT-PCR和Western-Blot方法检测乳腺癌组织中PNmRNA及蛋白的表达水平,并与正常乳腺组织的表达水平对照。采用免疫组织化学二步法(Envision法)检测不同临床分期乳腺癌组织、转移性淋巴结及良性病变中PN的表达情况。结果相对于正常乳腺组织,乳腺癌组织中PN在mRNA水平和蛋白水平均表达增高(均P<0.05),并随着肿瘤临床分期(TNM分期)的进展而表达增高。结论 PN在乳腺癌组织中表达上调,可能参与乳腺癌的病理发生发展过程。     

EGR1在乳腺癌组织中的表达及临床意义

目的:探究早期生长反应基因1（early growth response 1,EGR1）在乳腺癌组织中的表达及临床意义。方法:下载并提取TCGA数据库中EGR1的表达数据;购买乳腺癌组织芯片（HBreD090CS01）,并采用免疫组织化学法检测EGR1在乳腺癌中的表达;利用Kaplan-Meier Plotter数据库分析EGR1在乳腺癌中的预后价值;利用TIMER数据库分析乳腺癌中EGR1表达与免疫细胞浸润的关系。结果:相较于癌旁组织,EGR1 mRNA在乳腺癌中的表达显著下调（P＜0.001）;EGR1蛋白在45例乳腺癌组织中有18例呈低表达,27例呈高表达,在45例癌旁组织中有9例呈低表达,36例呈高表达,差异具有统计学意义（P=0.038）;EGR1表达与乳腺癌分化程度相关（P=0.003）,与患者年龄、肿瘤大小、N分期、临床分期等无关（P＞0.05）;EGR1低表达的乳腺癌患者,其无复发生存率显著低于EGR1高表达的患者（P＜0.001）,但EGR1表达与乳腺癌患者的总生存率无明显相关性（P＞0.05）;乳腺癌组织中EGR1表达与B细胞浸润水平呈负相关（P＜0.001）,与CD8+T细胞、CD4+T细胞、巨噬细胞、中性粒细胞、树突状细胞浸润水平呈正相关（P＜0.001）。结论:EGR1在乳腺癌组织中的表达较癌旁组织低,且与肿瘤分化程度、预后及免疫浸润有关,有望成为乳腺癌预后评估的分子标志物及治疗的潜在靶点。     

晚期胃癌血清可溶性Ｅ-钙粘连蛋白的表达及其意义

目的　观察晚期胃癌化疗前后血清可溶性Ｅ-钙粘连蛋白（ｓＥＣ）的浓度变化并探讨其临床意义。方法　用酶联免疫吸附方法检测４０例晚期胃癌初治患者接受ＦＯＬＦＯＸ４方案化疗前和化疗后第８周的血清ｓＥＣ浓度,分析化疗前后血清ｓＥＣ浓度及其变化与疗效的关系。结果　４０例患者获ＣＲ２例,ＰＲ１２例,ＳＤ１３例,ＰＤ１３例。获有效组（ＣＲ＋ＰＲ）患者化疗后的血清ｓＥＣ浓度为（２０８２.３６±５９０.３２）ｎｇ／ｍｌ,显著低于化疗前的（２６７４.５０±６８７.８９）ｎｇ／ｍｌ（Ｐ＜０.０５）;获ＳＤ和ＰＤ组患者化疗后血清ｓＥＣ浓度较化疗前均显著升高（Ｐ＜０.０５）;获ＳＤ和ＰＤ组患者化疗前基线ｓＥＣ水平显著低于有效组（Ｐ＜０.０５）。结论　化疗后血清ｓＥＣ浓度下降可能与晚期胃癌ＦＯＬＦＯＸ４方案化疗疗效呈正相关,化疗前ｓＥＣ浓度高的疗效好。     

E-cadherin在非小细胞肺癌中的表达及临床意义

目的探讨E-cadherin在非小细胞肺癌(NSCLC)中的表达.方法采用免疫组织化学SABC法,对常规10%福尔马林固定、石蜡包埋的NSCLC90例分别进行Ecadherin的检测.结果 E-cadherin在NSCLC中表达阳性率为52.2%(47/90).在NSCLC中E-cadherin与PTNM分期、病理分级、淋巴结转移及预后有显著相关性,与病理类型无关.结论 E-cadherin缺失可促使肿瘤转移,可能是NSCLC转移的重要因素之一,可作为判断NSCLC转移及预后有价值的指标之一.     

乳腺癌中c-erbB-2、EGFR的表达及其临床意义

目的 检测c—erbB-2、EGFR在乳腺癌中的表达及其预后的关系。方法 应用免疫组化Envision法对110例乳腺癌进行标记，分析c—erbB-2、EGFR、ER、PR的阳性表达情况。结果c—erbB-2、EGFR、ER、PR的阳性率分别为36．4％、45．5％、52．7％和47．3％，c—erbB-2、EGFR与ER、PR呈负相关；c—erbB-2、EGFR、ER、PR的表达与乳腺癌的组织类型、分化程度具有相关性。结论 乳腺癌中c—erbB-2、EGFR的表达提示乳腺癌患者预后不良，可作为临床判断预后的指标。     

前列腺癌E和N型钙粘附素的表达及意义

背景与目的：E－钙粘附素表达缺失与许多种癌密切相关,而最近在乳腺癌的研究中发现N－钙粘附素的反常表达在引起癌细胞的浸润及转移方面有着比E－钙粘附素缺失更为明显而直接的作用。本研究拟探讨前列腺癌中E和N－钙粘附素的表达与癌分级、分期及前列腺特异抗原（prostate specific antigen,PSA)的关系。方法：采用免疫组织化学染色法检测56例前列腺癌标本E和N－钙粘附素的表达。结果：E－钙粘附素阳性表达24例（43％）,阴性表达32例（57％）;N－钙粘附素表达阴性18例（32％）,阳性38例（68％）,E－钙粘附素表达缺失或N－钙粘附素阳性表达与肿瘤恶性程度及分期呈正相关（P均＜0．01）;与血清总PSA（T－PSA）和游离PSA（F－PSA）无明显相关性,而与F－PSA／T－PSA比值呈正相关（P＜0．01）。结论：E－钙粘附素和N－钙粘附素与前列腺癌的发生、发展明显相关,可以作为判断前列癌恶性程度及预后的指标。     

Biological and clinical significance of PARP1 protein expression in breast cancer

Breast Cancer Research and Treatment - Poly(ADP-ribose) polymerase-1 (PARP1) is a key facilitator of DNA repair. PARP inhibitors have gained recent attention as promising therapeutic agents for the...     

E-cadherinP120ctn在乳腺癌中的表达及意义

  目的  探讨E-钙黏蛋白(E-cadherin, E-cad)、P120连环蛋白(P120ctn)在乳腺浸润性导管癌(IDC)和浸润性小叶癌(ILC)中的表达及其鉴别诊断意义。  方法  收集解放军第113医院2007年12月至2012年5月间有完整随访资料的浸润性导管癌60例、浸润性小叶癌48例及混合性癌20例。采用免疫组织化学S-P法检测E-cad及P120的表达。  结果  E-cad在IDC和ILC中的阳性率分别为85%(51/60)和0, 其差异有统计学意义(P < 0.01);P120在IDC中阳性率为100%, 且均为细胞膜着色, 而在ILC中阳性率亦为100%, 但均为胞浆着色。E-cad和P120联合使用将20例混合癌确诊为IDC 16例及ILC 4例。IDC中E-cad的表达水平与肿瘤分期无关, 与淋巴结转移有关(P < 0.05)。  结论  E-cad和P120联合使用可以鉴别光镜下易混淆的IDC和ILC, 使组织分型更准确, 建议应用于乳腺癌的常规免疫组织化学检测。E-cad的表达水平与淋巴结转移有关。