银屑病患者循环免疫复合物及有关免疫指标观察

本文检查了109例寻常型银屑病住院病人的循环免疫、复合物及混合玫瑰花试验等几项免疫检查,提示银屑病可能是一种(皮肤)免疫复合物疾病. 用C₃b致敏酵母凝集试验检测39例银屑病病人,及用红细胞免疫试验检测19例,表明银屑病病人循环免疫复合物可以升高.免疫球蛋白检测显示IgG及IgA升高,以及C₃检测较正常值低,亦可支持银屑病与免疫复合物有关.用E. Y混合玫瑰花试验检测55例.结果B细胞减少,N及D细胞增多,结合文献复习,可以认为银屑病细胞免疫功能可能低下.且体液免疫功能亦可能有缺陷.PHA淋巴细胞转化率明显降低,也可说明细胞免疫功能低下.     

角蛋白、抗角蛋白自身抗体和银屑病

被作为上皮类型和分化阶段的分子标志——角蛋白,在银屑病患者表皮内明显地表现异常并且这种异常改变不仅仅出现在皮损区域。最新研究提示银屑病患者角朊细胞本身存在缺陷,它们较正常角朊细胞分化能力低下。直接以不同分子量的角蛋白作为对象的抗角蛋白自身抗体,也与银屑病有着明显的联系。有的角蛋白和抗角蛋白自身抗体可以作为判断银屑病的病情以及疗效、治愈标准的指标。深入研究银屑病患者角蛋白、抗角蛋白自身抗体的变化对进一步了解银屑病可能提供一些有益的启迪。     

采用聚乙二醇沉淀法和补体消耗法测定红斑狼疮的循环免疫复合物

系统性红斑狼疮(SLE)40例,其中狼疮活动者15例,非活动性者25例。慢性红斑狼疮13例,其中损害广泛分布者7例,局限性6例。在93.3%(14/15)的活动性SLE患者的血清中用聚乙二醇沉淀法找到免疫复合物型物质。在非活动性SLE中有此物质者44.0%(11/25),广泛性慢性红斑狼疮为83.3%(5/6),局限性慢性红斑狼疮42.9%(3/7)。试验结果说明存活动性SLE和广泛性慢性红斑狼疮中免疫复合物的阳性率均很高。此外在SLE聚乙二醇沉淀法和补体消耗试验二种方法所取得的结果相一致。而在慢性红斑狼疮中二种方法的测定结果无明显的平行关系。采用交联葡聚糖G—200的凝胶过滤法分别在1名SLE和1名广泛性慢性红斑狼疮患者血清的巨分子带发现数个抗补体峰,这些具有     

从麻风病人分离循环免疫复合物的补体激活作用

以往的研究已报告各型麻风病人循环免疫复合物(CICs)水平均有升高,但这些复合物在麻风的病理生理作用还未被研究,另外反应期与非反应期麻风病人的CICs在免疫病理学性质上是否有区别也属未知.本文的目的:(1)查证从各型麻风     

玫瑰糠疹患者血清中循环免疫复合物的测定

<正> 我们检测了玫瑰糠疹患者血清中的循环免疫复合物(CIC)。以探讨其发病机理。材料和方法研究对象玫瑰糠疹患者74例,献血员25例作对照。CIC测定按范纯武方法进行。CIC中的补体C_3 用PEG将血清中CIC沉淀下     

在银屑病、银屑病性关节炎中所含循环免疫复合物、血清免疫球蛋白和急性期蛋白质之临床意义

约7%的银屑病患者可发生银屑病性关节炎,其病因尚不明。免疫复合物曾在类风湿性关节炎病人的血清与滑液中检出,并在该病的发病机制上起一定作用。IgG 复合物亦曾在银屑病性关节炎的滑膜洗提液中测得,因此,这种复合物亦可能在银屑病性关节炎的发病机制中起到作用。作者乃用固相 Clq     

244例斑秃临床分析及自身抗体、免疫复合物检查

本文观察了斑秃患者244例,发现20～30岁为发病高峰年龄,有斑秃既往史及家族史者均显著多于对照组。50.5%患者发病前有精神因素。33.3%重型斑秃自身抗体阳性。这些结果提示斑秃发病与遗传,情神因素及免疫机能紊乱有关。固相C_(lq)酶联免疫法检查CIC未发现异常。     

寻常狼疮:循环和组织结合免疫复合物的测定

本文报告2例寻常狼疮病人,发现其皮损部真与表皮连接处有免疫球蛋白和补体沉积,其中1例测到循环免疫复合物增高。例1,46岁,女性。1979年3月,在左耳周围出现无症状的丘疹性损害,     

角蛋白,抗角蛋白自身抗体和银屑病

被作为上皮类型和分化阶段的分子标志－角蛋白，在银屑病患者表皮内明显地表现异常，并且这种异常改变不仅仅出现在皮损区域。最新研究提示屑病患者角朊细胞本身存在缺陷，它们较正常角朊细胞分化能力低下。直接以不同分子量的角蛋白作为对象的抗角蛋白自身抗体，也与银屑病有着明显的联系。有的角蛋白和抗角蛋白自身抗体可以作为判断银屑病的病情以及疗效、治愈标准的指标。深入研究银屑病患者角蛋白、抗角蛋白自身抗体的变化对进一     

银屑病患者外周血淋巴细胞的微核出现率

微核测定法显示40例银屑病之微核出现率为8.63%,较正常对照组明显增高,微核测定法为目前快速测定活体内染含体损伤的一种新方法.根据有关微核性质的现代概念.我们认为银屑病患者周围血淋巴细胞是存在染色体损伤的.     

Nickel sensitivity in atopics, psoriatics and healthy subjects

A prospective study of 552 persons was performed lo study nickel allergy in atopics, with and without dermatitis, psoriatics and healthy adults. We found no statistically-significant difference in the frequency of nickel allergy between persons with atopic dermatitis, atopics without dermatitis and healthy controls. More females than men gave a history of metal intolerance and gave allergic patch test reactions. A poor correlation between history and patch lest reaction was not specific for atopics. Psoriatics had a significantly lower frequency of allergic patch test reactions lo nickel than healthy controls or atopics with and without dermatitis. Psoriatics should not be used as controls for atopics in studies of contact dermatitis.     

Study of HLA class I,class II and complement genes (C2, C4A,C4B and BF) in Japanese psoriatics and analysis of a newly-found high-risk haplotype by pulsed field gel electrophoresis

Summary Genetic polymorphisms of HLA antigens and HLA-linked serum complement components (C2, C4A, C4B and BF) were investigated in 79 Japanese patients suffering from psoriasis. HLA typing revealed increased frequencies of HLA-A1, A2, B39, Bw46, Cw6, Cw7 and Cw11. Among complement components, positive associations were obtained with C4A4 and C4B2 and a negative association with BFF. The major histocompatibility complex haplotype (supratype), HLA-A2-Cw11-Bw46-C2C-BFS-C4A4-C4B2-DRw8 is purported to be a new high-risk haplotype in Japanese patients with psoriasis. Analysis of patients with this supratype via pulsed field gel electrophoresis showed the existence of specific, extensive DNA deletions near HLA-DR genes, but no disease-specific patterns could be observed by means of this technique. The newly-found high-risk haplotype indicates racial and ethnic differences among psoriatic patients.     

乙双吗琳治疗寻常型银屑病的初步临床观察报告

我科自1981年7月起使用由杭州第一制药厂提供的国产新药乙双吗琳胶囊,治疗寻常型银屑病94例,现将其中坚持服药,可供分析的60例临床观察资料,进行整理与分析报告如下.     

Treatment modalities of psoriatics over a 6-year period (1975-1981)

For an evaluation of the various treatments given to psoriatics, a total of 243 outpatients, all seen in 1975, were followed during a 6-year period. 18.1% underwent only one sort of treatment, 44.9% two sorts and 18.1% three sorts. The rest underwent four or more treatment modalities. Topical steroids were the most commonly used form of therapy given to 88.5%. Grenz-rays were given in small amounts to 73.3%, most often as a supplement to topical steroids. Methotrexate was given to 12.3%, PUVA to 13.6%, etretinate to 3.3% and UVB to 1.2%. 26.3% were treated as inpatients during the observation period, and finally 7.0% were treated at the Dead Sea.     

HLA-linked complement polymorphisms (C2, BF) in psoriasis

Summary Starting from the known association between psoriasis and several HLA antigens and from the fact that the HLA chromosomal region contains the structural genes for at least three complement components, the authors have looked for an association between psoriasis and allotypes of C2 and BF. C2 and BF polymorphism were examined in 230 psoriatic patients. Two rare complement genes were found to be significantly increased when compared with controls: the frequency of the C2 ^*2 gene was 0.061 among patients and 0.035 among controls (P<0.05); for BF ^*SO7, the frequencies were 0.0304 in patients and 0.0092 in controls (P<0.0005). The BF ^*F gene frequency, however, was significantly decreased among patients: 0.1196 vs. 0.1743 (P<0.01). The relative risks were 1.79 for the C22, 3.44 for the BF SO7, and 0.6 for the BFF gene product. From previous studies, it is known that these three complement alleles (C2 ^*2, BF^* SO7, BF ^*F) are in linkage disequilibrium with HLA alleles that have also been found increased or decreased, respectively, in psoriasis.Presented in part at the 4th International Congress of Immunology, Paris, July 21–26, 1980     

The fibrillarin (Scl-34) autoantibody in systemic scleroderma

Reexamination of ANA positive sera with nucleolare fluorescence staining on HEp-2 cells and hamster liver imprints revealed 3 sera with an uniform characteristic pattern. It is characterized by: 1. Strong clumpy fluorescence of nucleoli in the interphase. 2. Missing nucleoplasma staining (pure nucleolar). 3. Characteristic granular staining of the cytoplasma from the condensed chromosoma in mitosis. 4. Additive fluorescence of a nucleolus-like body. 5. Extremely high antibody titers. 6. Negative immunodiffusion. This nucleolar staining pattern was described by Bernstein et al. as a clumpy nucleolar pattern. It is produced through an antibody to the dense fibrillar component of the nucleolus (fibrillarin). This antigen is a protein of the U3 RNP with a molecular weight 34 kDa. Clinically is the fibrillarin antibody highly specific for systemic scleroderma especially with diffuse skin involvement. We found it in 1% of scleroderma patients. Its pathogenetic importance is not known.     

Immunoglobulin and complement deposits in the skin and circulating immune complexes in scabies

Sixteen patients with papulovesicular, 6 with nodular and one with a Norwegian scabies were studied. Direct immunofluorescence (IF) examination revealed C3 deposits in the skin lesions of 13 of the 18 patients. Among them were all 6 cases with nodular scabies. C3 was found mostly in dermal vessel walls and 3 of the patients also showed IgM and 2 IgA deposits at the same site. No circulating immune complexes were found, with a solid-phase C1q radioimmunoassay (RIA), but HSV- and RSV-RIA methods detected IgM antibodies of rheumatoid factor type in 5 of the 15 sera examined. These results suggest that local complement activation and perhaps also immune complex deposition may by important in the pathogenesis of the papular and nodular skin lesions of human scabies.