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1.
黄芪多糖对IL-2/LAK抗肿瘤增强作用的动物实验研究   总被引:3,自引:1,他引:3  
采用C57BL/6鼠荷其自发产生的黑色素瘤B_16细胞,以生存期为指标,建立了黄茂多糖(APS)增强IL-2/LAK抗肿瘤作用的动物模型,结果表明:APS自身具有一定的抗肿瘤作用,APS(5mg/kg)与IL-2/LAK(5×10 ̄3U/kg、5×10 ̄7/kg)的抗肿瘤作用相似,荷瘤鼠生存期分别为21.57±1.81天和20.86±1.86天(荷瘤对照鼠为15.71±0.49天);APS对IL-2/LAK的抗肿瘤效应有明显的增强作用,二者在上述剂量下联合应用,荷瘤鼠生存期为24.86±2.73天(P<0.01)。并对荷瘤鼠进行动态细胞免疫功能观察,提示:APS和IL-2/LAM均具有抵抗鼠脾NK细胞活性,IL-2产生能力和腹腔Mψ吞噬能力下降的作用,APS对IL-2/LAK仍有显著增强效应。  相似文献   

2.
锂增强小鼠LAK细胞活性及其体内抗瘤作用的研究   总被引:5,自引:0,他引:5  
本文观察了锂体外对小鼠LAK细胞的作用及其体内抗瘤作用,以期找到一种能增强LAK细胞活性,降低IL-2毒副作用的免疫调变剂。在IL-2诱导LAK的同时加入LiCl,则能增强LAK细胞的活性。以C57BL/6小鼠接种B16黑色素瘤细胞为荷瘤小鼠模型,比较了IL-2/LAK,单独锂及IL-2/LAK合并锂三组的抗瘤作用,结果表明,单独锂及IL-2/LAK组与对照组相比均有抗瘤作用,但IL-2/LAK和锂一起作用,其抗瘤作用最强,表现为能抑制肿瘤的生长及延长荷瘤小鼠的存活时间。因此,锂可望作为一种新型的免疫调节剂用于免疫性疾病及肿瘤的治疗。  相似文献   

3.
观察了小鼠接种高分泌IL-4的黑色素瘤细胞后体内免疫效应细胞(包括CTL、NK细胞及LAK细胞)抗肿瘤活性及其分泌的细胞因子(包括IL-2、IFN-r、TNF及GM-CSF)水平的变化,荷瘤后第15天小鼠脾细胞NK活性及经诱导后的LAK活性有所降低,而经诱导后的CTL杀伤活性显著升高,荷瘤小鼠腹腔内巨噬细胞杀伤活性及其分泌的IL-1水平也明显升高;在荷瘤后第4天及第12天出现两个高峰,实验结果表明,高分泌IL-4的黑色素瘤细胞体内生长受抑制是因为IL-4基因的导入及IL-4的分泌使体内CTL及巨噬细胞杀伤活性提高,因而使机体抗肿瘤免疫功能得以增强。  相似文献   

4.
绿茶儿茶素体内增强小鼠的肿瘤免疫反应   总被引:11,自引:0,他引:11  
研究绿茶儿茶素(Greenteacatechin,GTC)在小鼠体内对淋巴瘤L5178Y免疫反应的影响。结果表明,GTC能(1)促进对L5178Y产生免疫反应的小鼠细胞的特异性增殖,这一作用在GTC2mg/d灌喂的小鼠尤为显著,增殖作用比不灌喂的高6~10倍;(2)加强特异性CTL的诱导;(3)增加IL-2分泌和IL-2m-RNA表达;(4)培强NK细胞活性。GTC上述作用可能与降低巨噬细胞的抑制  相似文献   

5.
TNF通过自分泌方式促进人T-LAK细胞体外增殖和功能分化[英]/InninsEK…//Cytokine.-1992,4.-39~396用IL-2刺激的人FBMC能释放TNF等细胞因子。本实验首次检测了内源性TNF对人T-LAK(T细胞衍生的)细胞增...  相似文献   

6.
慢性活动性乙型肝炎患者细胞免疫功能检测及其临床意义   总被引:26,自引:0,他引:26  
测定了例慢性活动乙型肝炎患者外周血单个核细胞(PBMC)产生的白细胞介素2(IL-2)活性,其中部分病人检测了血清可溶性白细胞介素2受体(sIL-2R)水平,膜白细胞介素2受体(mIL-2R)表达,LAK活性及外周血T淋巴细胞亚群,并分析了它们之间的相关性,结果表明:IL-2活性,mIL-2R表达,LAK活性,CD4/CD8比值显著低于正常对照组(P〈0.001),而sIL-2R水平,CD8细胞显  相似文献   

7.
本研究用重组白细胞介素2(rIL-2)联合转移因子(TF)或抗肿瘤免疫核糖核酸(iRNA)作用于外周血单个核细胞(PBMC),测定其对K562,Raji及H7404细胞的杀伤活性。结果发现,高浓度TF(0.25u/ml)可抑制LAK活性,TF进一步稀释(0.125u/ml)可使其抑制作用消失。TF和抗肿瘤iRNA不能进一步提高最适剂量rIL-2(500u/ml)诱导的LAK活性,但能显著增强亚适剂量rIL-2(200u/ml)诱导的LAK活性,诱导LAK活性增高的TF最适剂量为0.031u/ml。TF或抗肿瘤iRNA单独不能诱导出LAK活性,而当两者联合应用可诱导PBMC产生LAK活性。本文为TF及抗肿瘤iRNA协同LAK细胞疗法在临床应用提供实验基础。  相似文献   

8.
采用编码人IL-2基因的重组痘苗病毒载体(rVV-IL-2),以invivo模式体内局部转染小鼠黑色素瘤,结果肿瘤生长速度明显减慢,荷瘤小鼠存活期显著延长。体内免疫功能的检测表明,荷瘤小鼠脾细胞的NK活性及诱导后的LAK、CTL活性明显高于对照组,表明采用rVV-IL-2载体介导invivo的肿瘤基因治疗具有一定的前景。  相似文献   

9.
IL-2基因修饰成纤维细胞对肝癌荷瘤小鼠的治疗实验   总被引:2,自引:0,他引:2  
目的研究双顺反子逆转录病毒载体介导IL-2基因转导成纤维细胞对小鼠肝癌的治疗作用。方法利用双顺反子逆转录病毒载体pGCEN/IL-2将IL-2基因转导小鼠成纤维细胞NIH3T3,然后将分泌IL-2的成纤维细胞与60Gyγ射线照射的肝癌H22细胞皮下植入三天前5×105或2.2×105肝癌H22细胞皮下注射的BALB/c鼠。结果分泌IL-2的成纤维细胞治疗组能明显增加荷瘤小鼠的生存率(P<0.025),抑制肿瘤生长(P<0.05),在肿瘤植入第70天无肿瘤形成的小鼠再次注射1×106H22细胞仍然不发生肿瘤。51Cr释放试验表明来自照射H22与分泌IL-2成纤维细胞混合液注射的小鼠脾细胞与51Cr标记的H22细胞共培养后,51Cr特异释放明显高于对照组(P<0.05)。结论实验结果表明IL-2基因修饰的成纤维细胞能诱导针对肝癌的特异免疫反应。  相似文献   

10.
人白细胞介素4诱导杀伤细胞的研究   总被引:3,自引:2,他引:3  
人重组白细胞介素4(rhIL-4)在PHA协同下,从人外周血单核细胞(PBMC)诱导出明显的LAK活性,其对K562、Raji细胞的杀伤力低于IL-2诱导者,对TBL-E,PHA活化的淋巴母细胞(PHA-blasts)的杀伤力和IL-2诱导者相似。在PHA介导的4小时51Cr杀伤试验中,加入PHA后,IL-4-LAK对PHA-blasts的杀伤力提高2.3倍,而IL-2-LAK对PHA-blasts的杀伤力无变化,提示IL-4主要诱导CTL样活性,而IL-2主要诱导NK样活性,IL-4诱导效应CTL的能力强于IL-2。我们的实验同时证实,在淋巴细胞活化的早期,IL-4抑制IL-2诱导的LAK活性,淋巴细胞活化后,IL-4与IL-2有协同作用,增强IL-2诱导的LAK活性。  相似文献   

11.
Differential bioassay of interleukin 2 and interleukin 4   总被引:5,自引:0,他引:5  
The T cell-derived lymphokines interleukin 2 (IL-2) and interleukin 4 (IL-4, originally BSF-1) both exhibit T cell growth-promoting activity. Recent observations that T cell lines commonly used as indicator cells in IL-2 bioassays also proliferate in response to IL-4 demonstrate the lack of specificity of these bioassays for IL-2. In this report we describe a bioassay method to differentiate IL-2 and IL-4 through the parallel use of two T cell lines with defined lymphokine specificity. The IL-2-responsive CT6 cell line, when used in conjunction with the IL-2- and IL-4-responsive HT-2 cell line, allows for the differentiation of IL-2 and IL-4 in conditioned media.  相似文献   

12.
Solid phase interleukin 2   总被引:1,自引:0,他引:1  
D R Kaplan 《Molecular immunology》1991,28(11):1255-1261
Interleukin 2 was bound to Sepharose beads in order to assess its potential bioactivity in vitro. Although the cytokine was attached to the solid phase via a standard chemical reaction for covalent coupling, it was spontaneously released into culture medium in an active form during short-term incubation. Release was absolutely dependent on the availability of soluble protein in the incubation medium, and it was greater at 37 than at 4 degrees C. Approximately 80% of the cytokine attached to the Sepharose beads was recovered in the medium after appropriate incubation for several days. The release of interleukin 2 from the solid phase resulted from the dissolution of aggregated cytokine. Noncovalent self-aggregation of interleukin 2 onto solid surfaces represents an unusual biochemical property that has implications for the molecular interactions of the cytokine with the solid phase, for the use of bound interleukin 2 in preparative or analytical modes, and for the development of immunotherapy for patients with solid tumors.  相似文献   

13.
K N Lai  J C Leung  F M Lai 《Pathology》1991,23(3):224-228
Following activation in vitro, peripheral blood mononuclear cells (PBMC) express cell-associated interleukin-2 receptors (IL2R). The present study was undertaken to define the proportion of T lymphocyte subsets that express the IL2R (CD25 antigen) upon different mitogenic stimulation. Double immunofluorescence staining with different fluorochromes, fluorescein isothiocyanate and phycoethyrin, was applied for identification of IL2R positive cells and individual lymphocyte subset. The exact percentage of individual activated lymphocyte subset bearing IL2R was enumerated by photographic counting. There was paucity of IL2R in freshly isolated, unstimulated peripheral blood, PBMC cultured without mitogen, and cultured B lymphocytes. Following pokeweed mitogen stimulation in vitro, 19% of CD4 (T-helper/inducer) lymphocytes and 14% of CD8 (T-suppressor/cytotoxic) lymphocytes expressed IL2R. Similarly, 25% of CD4 lymphocytes and 19% of CD8 lymphocytes expressed IL2R following phytohemagglutinin stimulation in vitro. Contrary to the reported data of Tac-positive cells in human lymphoid tissues, our study revealed that, upon lectin mitogen stimulation, approximately 55% of IL2R positive PBMC were CD4 lymphocytes, and 45% of them were CD8 lymphocytes. These observations imply the plausible notion that interleukin-2 mediated immune activation of T lymphocytes in PBMC is different from that in local lymphoid organs. It was also demonstrated that the release of soluble IL2R (sIL2R) and IL2 production in supernatant from cultured PBMC varied with different lectin stimulation. A significant correlation was demonstrated between the cellular and soluble IL2R but the production of IL2 from activated mononuclear cells bore no good correlation with either the cellular IL2R expression or the release of sIL2R.  相似文献   

14.
PHA activated human peripheral blood mononuclear cells (MNC) were incubated with human interleukin 2 (IL-2) in the absence or in the presence of 10(-6)-10(-9) M hydrocortisone (HC). HC suppressed the proliferative response of the activated MNC to highly purified human leucocyte IL-2 in a dose dependent manner. This suppression was in full accordance with an inhibition of the IL-2 binding capacity, whereby the high affinity binding sites were reduced by 85%, while the low affinity sites were less affected. The mechanism by which HC inhibits the binding of IL-2 is still unknown. Evidence is presented that HC binds only weakly to IL-2. We conclude that HC interferes with the IL-2 binding by modulating the binding and/or signal processing function of the IL-2 receptor.  相似文献   

15.
Murine interleukin 2 receptors found on freshly isolated and on in vitro activated lymphocytes were identified using a fluorescent interleukin 2 (IL2F). Three percent of freshly isolated small thymocytes bound the IL2F; these cells appeared to be dual CD4 and CD8 positive cells. Ten percent of the larger thymocytes also bound the IL2F; phenotypically, these cells were more heterogenous in their CD4/CD8 composition than the small IL2F+ thymocytes. Freshly isolated splenocytes bound more IL2F than did the thymocytes. Twenty-four percent of the small splenocytes were IL2F+ and they were mostly B220+ cells. Half of the larger splenocytes were IL2 receptor positive and these cells consisted of B and T cells. Using mitogen stimulated splenocytes, three times as many LPS stimulated B220+ blasts bound the fluorescent IL2 than freshly isolated large B220+ cells; this level of IL2F binding was maintained for four days. Of the Con A blasts, more CD8+ cells (30%) bound IL2F than did CD4+ blasts (19%); these cells maintained this level of IL2F binding for only three days. The IL2F binding could be completely inhibited by excess unlabeled IL2 and could be inhibited by 92% using a monoclonal antibody directed against the IL2 binding region of the IL2 alpha receptor, indicating that IL2F can bind to both IL2 alpha and IL2 beta receptors.  相似文献   

16.
17.
The immunosuppressive effects of a recombinant soluble IL-2 receptor L chain (s-IL-2R) were analyzed. S-IL-2R protein was obtained from the conditioned medium of L cells transfected with a mutant cDNA clone encoding the extracytoplasmic portion of the IL-2 receptor (IL-2R) and was purified to homogeneity by an IL-2-coupled sepharose column, following by reverse phase chromatography (HPLC). Soluble IL-2R protein thus prepared retained the ability to bind IL-2 specifically and suppressed the in vitro IL-2-mediated immune responses, including proliferation of IL-2-dependent cell line (CTLL-2), induction of secondary cytotoxic T lymphocytes (CTL) and the mixed lymphocyte reaction (MLR), but did not suppress the growth of IL-3-dependent cell line. Kinetic studies revealed that s-IL-2R exhibited the suppressive effects on the proliferative responses of alloantigen stimulated human tonsillar cells, only when added at an early stage, namely 0-48 h after culture onset, whereas cyclosporin A (CsA) exhibited an inhibitory effect only when added at between 0 and 24 h. This implies that s-IL-2R exerts its effect on an early stage of lymphocyte activation. The observed immunosuppressive effects of s-IL-2R suggest the possibility that s-IL-2R might be useful for the protection of rejection crisis in organ transplantation.  相似文献   

18.
Augmentation of interleukin 1 and interleukin 2 production by OK-432   总被引:2,自引:0,他引:2  
Intraperitoneal (i.p.) administration of OK-432 augmented both interleukin 1 (IL-1) and interleukin 2 (IL-2) production to the rechallenge of OK-432 in vitro. Peritoneal exudate cells (PEC) of mice 8 days after i.p. injection with OK-432 (1 KE/mouse) showed maximum IL-1 production to the restimulation with OK-432 in vitro. OK-432-induced IL-1 was consisted of three molecular weight species (two major peaks: 85 K and 15 K daltons and one minor peak: 67 K daltons) on Sephadex G-100 chromatography. Splenocytes of mice 4 days after i.p. injection with OK-432 (1 KE/mouse) demonstrated maximum IL-2 production to the in vitro rechallenge of OK-432, however, in vivo OK-432 administration failed to enhance ConA-induced IL-2 production in vitro. From gel filtration analysis, OK-432 induced IL-2 had an unique molecular weight (approximately 70 K daltons). From these results, OK-432-induced augmentation of cellular immunity against tumor cells might be due to the activation of so-called lymphokine cascade reaction mediated by IL-1 and IL-2.  相似文献   

19.
The present study was undertaken to examine the T-lymphocyte activation in IgA nephropathy. Serum-soluble interleukin 2 receptor (sIL2R) levels were studied in 29 IgA nephritic patients, 17 patients with chronic glomerulonephritis (non-IgA nephropathy), and 30 healthy controls during an infection-free period. No difference in serum sIL2R level was demonstrated among these three groups of subjects. However, the serum sIL2R levels of IgA nephritic patient rose significantly during clinical exacerbation with synpharyngitic macroscopic hematuria and the serum sIL2R levels fell when hematuria subsided. Mitogen-stimulated cellular interleukin 2 receptor (IL2R) expression, sIL2R release, and interleukin 2 (IL2) production were also examined in peripheral blood mononuclear cells (PBMC) cultured for 24–48 hr in 21 patients with IgA nephropathy, 17 patients with chronic glomerulonephritides, and 17 healthy controls. The total cellular IL2R expression and sIL2R release did not differ among these three groups of subjects. However, the individual T-cell subsets bearing IL2R were distinctly different between IgA nephritic patients and the other two groups of controls. IgA nephritic patients had increased activated CD4+ lymphocytes and reduced activated CD8+ lymphocytes. Furthermore, IL2 production in response to phytohemagglutinin and pokeweed mitogen stimulation was increased in lymphocytes from patients with IgA nephropathy. The IL2 production did not correlate with the quantities of cellular and sIL2R yet the cellular IL2R expression paralleled the sIL2R released by cultured lymphocytes. Our present study suggests that the T lymphocytes from patients with IgA nephropathy have a defect in overproduction of IL2 and increased activated T helper-cell subset upon mitogenic stimulation. Serum measurement of sIL2R could potentially be useful in monitoring the disease activity.  相似文献   

20.
Interleukin 2 (IL-2) is a class of T cell growth factors produced by T cells of a number of species. The unique growth-promoting properties of these molecules allow the development of antigen-specific effector T cell lines which can be used to analyze the molecular basis of lymphocyte interactions. A murine T cell tumor line has been identified as a source of IL-2. The purification and biochemical properties of murine IL-2 are described, and compared with rat and human IL-2.  相似文献   

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