乳牙菌斑液无机阳离子含量与龋易感性的关系

目的　分析乳牙菌斑液中无机阳离子含量与龋易感性的关系。方法　将 4 1名 3～ 5岁儿童 ,按龋齿情况将其分为 3组 :无龋组dft=0 ,CSI=0 ,n =2 0 ;龋低危组 0 10 ,n =10。用离子色谱测定各受试者息止菌斑液中无机阳离子浓度。结果　无龋组与高危组、低危组与高危组钠离子、镁离子水平有显著性差异 (P <0 .0 5 ) ,镁离子与dft、CSI有正相关性 (r分别为 0 .5 6 ,0 .6 4 )。结论　提示乳牙息止菌斑液镁离子浓度与乳牙龋病、机体龋敏感度的有关     

乳牙菌斑液磷酸根浓度及其与患龋状况关系的分析

目的 :探讨乳牙菌斑液中磷酸根浓度与儿童患龋状况的关系 ,为乳牙菌斑液及龋病的研究提供基础资料。方法 :对 46名 3～ 5岁儿童 ,用离子色谱仪测定集合菌斑液的磷酸根浓度 ,并检查其患龋状况 ,统计分析乳牙菌斑液磷酸根浓度与儿童患龋状况之间的关系。结果 :乳牙菌斑液磷酸根浓度在无龋组为 (12 .63± 2 .91)mmol/L、有龋组为 (13 .85± 3 .2 8)mmol/L、其中龋病高危组为 (14 .62± 3 .47)mmol/L ,无龋组与有龋组之间无显著性差异 ,无龋组与龋病高危组之间无显著性差异。乳牙菌斑液磷酸根浓度与dft、dfs、和CSI无相关性。结论 :乳牙集合菌斑液磷酸根浓度与儿童患龋状况无关 ,菌斑液中单一成分不能反映菌斑的致龋能力。     

无口腔保健干预牙菌斑液成分的研究

目的 研究长期无口腔保健干预的牙菌斑液粘代谢规律及其致龋力。方法 选择长期不刷牙者４３人,分为无龋组２７人,高龋组１６人,采集１０％蔗糖漱口前后的菌斑,检测菌斑提取液的ｐＨ值、有机酸和无机离子的浓度。结果 糖漱口后,长期菌斑提取注的ｐＨ值,、铵离子浓度降低,乳酸、甲酸升高,与禁食后新形成菌斑的糖代谢规律一致。但糖漱口前后两组菌斑提取液中的单一成分差异均无显著性。结论 长期菌斑与短期菌斑的糖代谢规律     

菌斑原位pH在判断个体龋易感性中的作用

口内牙齿表面菌斑的原位pH既能反映细菌代谢产酸的能力,也反映局部环境缓冲和转运酸的能力。本研究使用钯-微触电极测量龋活跃者(CA)和无龋者(CF)在用10％蔗糖溶液漱口前后菌斑的原位pH,以评价原位pH测定在判断个体龋易感性中的作用。结果表明,龋活跃组菌斑pH在漱糖前后各时间点均较无龋组低;龋活跃组与无龋组在漱糖后菌斑下降的最低pH相近,但龋活跃组菌斑pH回升速度慢,导致低pH水平持续时间长。上颌牙菌斑pH水平较下颌牙菌斑pH水平低,而左右侧牙菌斑pH水平大致相同,提示唾液在维持菌斑pH水平中的主导作用。     

牙菌斑液对牙釉质矿物的饱和程度与龋活动性的关系

为了解菌斑液对牙齿矿物饱和度随糖漱口的变化，以及与龋活动性的关系，作者选择２２例年龄相同的大学生，按龋齿情况，将其分为３组。第１组无龋（无龋组），第２组ＤＭＦＳ大于１０但无活动性龋（普通龋组），第３组ＤＭＦＳ大于１０且有活动性龋（活动性龋组），测定各受试者在静止时、用蔗糖液漱口后３ｍｉｎ和７ｍｉｎ时的菌斑液中有机酸、ｐＨ值、钙离子活度（有效离子浓度）和无机成分的含量，并计算对于釉质矿物的饱和度。结果表明，糖漱口之后菌斑液乳酸浓度升高、ｐＨ值降低、总钙和离子钙浓度增加。活动性龋组的菌斑液在糖漱口后ｐＨ值和饱和度降低的幅度明显大于无龋组和普通龋组。结论：牙菌斑液对牙齿矿物的饱和度是指示个体接受致龋食物后牙齿脱矿倾向的敏感指标。     

乳牙菌斑钙含量与患龋状况关系的研究

目的:分析乳牙集合菌斑中钙含量与儿童患龋状况的关系,为龋病预防研究提供基础资料。方法:应用原子吸收分光光度仪测定53例不同患龋状况的儿童集合菌斑中钙含量值。统计分析集合菌斑中钙含量值与龋病易感性的关系。结果:集合菌斑钙含量为(0.931±0.384)mg/g,菌斑钙含量无龋组、有龋组间差异有高度显著性P<0.01;无龋组、低危组、高危组间差异有显著性P<0.05,菌斑钙含量无龋组高于低危组,低危组高于高危组;有龋组集合菌斑中钙含量与龋补牙数(dft),龋补牙面数(dfs),龋蚀指数(CSI)具有负相关性。结论:集合菌斑中总钙含量与龋患程度具有一定的相关性,可为预测龋病易感性提供参考。     

菌斑pH值、游离钙和总蛋白水平与根龋易感性的关系

目的　分析菌斑液成分的动态变化与根龋易感性的关系。方法　比较无根龋组和有根龋组根面牙菌斑液在糖溶液漱口前后 ,所测定pH值、游离钙 (微电极法 )和总蛋白水平 (分光光度法 )的差异。结果　静止状态下 ,无根龋组和有根龋组的pH值 (无根龋组 6 2 4± 0 74,有根龋组 5 96±0 70 )和游离钙 [无根龋组 (0 6 0± 0 46 )mmol/L ,有根龋组 (0 89± 0 5 4)mmol/L]两项指标差异无统计学意义 ;糖漱口后 ,有根龋组的pH(5 14± 0 19)和游离钙 [(1 73± 0 74)mmol/L]水平均低于无根龋组[pH 5 2 8± 0 16 ,游离钙 (2 73± 1 2 5 )mmol/L],差异有统计学意义。总蛋白水平与游离钙含量之间没有相关性。同一个体牙根面与冠面之间菌斑液的三项指标均有显著的相关关系。结论　有根龋组的菌斑液有较强的致龋倾向 ,在细菌酵解糖产酸方面 ,牙根面 (牙骨质 )菌斑与牙冠面 (牙釉质 )菌斑无根本差别     

牙菌斑液成分在判断个体龋易感性中的作用

目的研究糖代谢后牙菌斑液化学成分与龋的关系,探求判别个体龋易感性中的因子.方法6～7岁儿童,无龋(dmfs=0)12名,高龋(dmfs≥10)6名,低龋(3≤dmfs＜10)7名.毛细管电泳技术(Beckman,Pace5000)检测有机酸、磷酸和无机阳离子.结果无龋组乳酸和低pKa酸的水平显著低于低龋组(P=0.002)和高龋组(P=0.01).多元逐步回归法和Logist回归法筛选出乳酸为龋的判别因子(P=0.0644).结论糖代谢7min后菌斑液中乳酸浓度的变化具有判断个体对龋易感程度的作用.     

乳牙菌斑液pH值与患龋状况及摄糖频率的关系

目的分析乳牙菌斑液pH值与患龋状况及摄糖频率之间的关系。方法将93名北京城区3～5岁儿童，根据患龋情况分为重症婴幼儿龋（Severe Early Childhood Caries，S—ECC）组47名，dt≥5；无龋（Caries Free，CF）组46名，dmft=0。分别收集集合菌斑，应用离子选择性微电极法测定受试者乳牙菌斑液pH值，并进行组间比较；对两组受试者每日摄糖频率进行调查，分析菌班液pH值与摄糖频率的关系；并在S—ECC组分析菌斑液pH值与dt的关系。结果S—ECC组菌斑液pH均值低于CF组，差别有统计学意义；S—ECC组患儿每日摄糖频率高于CF组；乳牙菌斑液pH值与摄糖频率呈负相关关系；S—ECC组菌斑液pH值与dt相关（P〈0、05）。结论乳牙菌斑液pH值与摄糖频率呈负相关关系，并且S—ECC组儿童与CF组儿童相比，其菌斑液有更大的致龋潜能。     

乳牙集合菌斑中钙含量与患龋状况的关系

菌斑内不断进行的钙磷代谢活动是釉质脱矿和再矿化的基础 ,和龋病发生发展密切相关。我们测定集合菌斑中钙含量值 ,比较不同龋患人群菌斑钙含量的差异 ,探讨龋病易感性与菌斑钙含量的关系 ,寻求筛选龋病易感人群的方法。1 对象 :受检者为 3～ 5岁乳牙列龋患者 5 3例。按记录下的口腔患龋状况计算乳牙列的龋蚀指数 (cariesseverityindex ,CSI)。以龋患程度为指标分组 :无龋组 2 1例 (CSI =0 ) ;有龋组 32例 ,其中包括低危组 17例 ( 0 相似文献   

澳门幼儿乳牙龋病及龋病活跃性的分析研究

目的：了解澳门地区幼儿乳牙患龋状况及其龋病活跃性,并作两者间关系分析,填补该地区资料的空白。方法：对澳门生活环境、经济条件等有较大差异的两所幼儿园2～4岁930名幼儿作龋蚀程度检查和记录;其中910名幼儿并作龋病活跃性检测SCAT,依据牙菌斑产酸能力强度分度。统计分析患龋率,龋均dft、龋蚀严重度指数CSI和SCAT。结果：①2～4岁930名幼儿乳牙患龋率为42.5%、dft1.93、CSI6.35,性别间无显著性差异（P〉0.05）。②患龋率、dft、CSI在年龄组间均有显著性差异。③两幼儿园间,患龋率、dft、CSI均有明显差异。④910名幼儿SCAT0度和1度占68.68%,2度和3度占31.32%。⑤两幼儿园间SCAT各度的分布有明显差异。⑥SCAT各度间,患龋率、dft、CSI均有明显差异,dft、CSI与SCAT相关性有显著意义。结论：①澳门幼儿患龋状况不容忽视。应抓紧低年龄幼儿的龋病防治工作。②儿童所处的不同生活条件可影响患龋状况,普及科普知识、维护口腔卫生、开展定期防治等的推广尤有必要。③SCAT能反映患龋状况的敏感度,在筛选幼儿龋病高危群体、有的放矢开展龋病防治计划工作中,既能节省人力、财力、又能提高效率。     

Composition and buffer capacity of pooled starved plaque fluid from caries-free and caries-susceptible individuals

The composition of pooled starved plaque fluid from six population samples, grouped according to age (8-11, 12-16, 8-17, 18-22 years) and caries status (caries-free, CF, DMFS = 0; caries-susceptible, CS, DMFS greater than 10), was determined by means of ion chromatography. Prior to plaque collection, all subjects abstained from oral hygiene for 48 hours and fasted overnight. In addition, selected subjects also received a dental prophylaxis one week prior to plaque collection. Plaque samples from each group were pooled under mineral oil and centrifuged at 15,000 g (ambient temperature) for 45 minutes. Supernatants were then analyzed for organic acids, inorganic anions, and mono- and divalent cations. Plaque fluid samples from CF and from the CS subgroups were also titrated with standard HCl for exploration of the inherent buffer capacity. The ions Na+ NH4+, K+, Ca2+, Mg2+, Cl-, phosphate, and carbonate were present in all samples analyzed. Formic, acetic, propionic, butyric, and trace amounts of isobutyric and isovaleric acids were detected, with acetic and propionic acids comprising between 85 and 94% of the total acids. Little (less than 1 mmol/L) to no succinic or lactic acid was detected in starved plaque fluid. No apparent difference was detected in the composition and saturation status of pooled starved plaque fluid from CF and CS individuals. These results suggest that caries susceptibility is not necessarily correlated with the plaque fluid composition of pooled starved plaque. In addition, no apparent difference in the inherent buffer capacities of these samples could be detected, consistent with the noted similarities in composition.(ABSTRACT TRUNCATED AT 250 WORDS)     

Relationship among salivary carbonic anhydrase VI activity and flow rate, biofilm pH and caries in primary dentition

This study aimed to determine the activity of carbonic anhydrase isoenzyme VI (CAVI) in the saliva of preschool children with caries and to investigate the relationship between caries and salivary CAVI activity, salivary flow rate and biofilm pH before and after a 20% sucrose rinse. Thirty preschool children aged 45.3-80.3 months were divided into two groups: a caries-free group and a caries group. Clinical examinations were conducted by one examiner (κ = 0.95) according to WHO criteria (dmfs) and early caries lesions. From each subject, CAVI activity, salivary flow rate and plaque pH were determined before and after a sucrose rinse. The results were submitted to Wilcoxon, Mann-Whitney and Spearman correlation tests (α = 0.05). The results showed that prerinse CAVI activity and its variation were higher in the saliva from caries children than from caries-free children. No difference was found between the two groups in postrinse salivary CAVI activity. After rinsing, biofilm pH differences were lower in both groups (p = 0.0012 and p = 0.0037 for the caries and caries-free groups, respectively). Also, after the sucrose rinse, salivary flow rate significantly increased in caries and caries-free groups (p = 0.0003, p = 0.0037). The variation of salivary CAVI activity was negatively correlated with caries (r = -0.501, p = 0.005). Child's age showed a positive correlation with caries (r = 0.456, p = 0.011). These results suggest that variation of salivary CAVI activity and child's age are associated with dental caries in preschool children.     

Importance of high pKA acids in cariogenic potential of plaque

Through the use of computer simulation, it is shown that anions of high pKA acids (e.g., acetic, propionic, butyric), present in resting plaque fluid, act as effective buffers during the production of stronger acids (e.g., lactic) by oral micro-organisms. Using reported organic acid compositions for plaque, obtained from caries-resistant and caries-susceptible individuals, analyzed at various times following sucrose exposure, it is shown that the calculated pH values of the aqueous phase of plaque describe typical Stephan curves. Furthermore, it is shown that for higher acid anion contents in resting plaque, a higher degree of saturation of the aqueous plaque phase with respect to enamel is maintained during acid production, resulting in a lower demineralization potential of plaque. Enamel demineralization experiments conducted in vitro confirmed the significance of the results of the computer simulations, thus providing evidence for a correlation between the known metabolic activity of plaque (organic acid composition), physical chemistry of plaque, and caries susceptibility.     

Association of caries activity with the composition of dental plaque fluid

This study tests the hypothesis that caries activity is associated with lower degrees of saturation with respect to enamel mineral in dental plaque fluid following sucrose exposure. Plaque fluids were obtained from caries-free, caries-positive, and caries-active subjects. Samples were collected before and at 3 and 7 min after a sucrose rinse on consecutive weeks and analyzed for organic acids, inorganic ions, pH, calcium activity, and, in selected samples, total protein. After sucrose, pH values were significantly lower in the caries-active group in comparison with the caries-free and caries-positive groups. Total and free calcium concentrations increased with decreasing pH, with free calcium being about one-third of total calcium. The caries-active group exhibited significantly lower degrees of saturation with respect to enamel mineral, after sucrose, and had significantly higher mutans streptococci levels in plaque than did the caries-free samples. Thus, saturation levels in post-sucrose plaque fluids reflect the cariogenic potential of dental plaque.     

唾液及菌斑液中蛋白成分与龋易感性的关系

目的：探讨纯腮腺液，全唾液及菌斑液中蛋白成分与龋易感性的关系。方法：选39名无龋者（caries free,CF)和27例高龋者（caries susceptible,CS)，用Folin酚法测定纯腮腺液，全唾液及菌斑液的总蛋白含量，SDS－PAGE和图像分析系统定量分析各蛋白成分。结果：总蛋白含量菌斑液高于唾液近10倍，其中仅CF组全唾液与菌斑液有较大相关性（r=0.804).3种液体共有的蛋白为14000，66000及76000蛋白，菌斑液中14000，15000及38000蛋白和全唾液中14000蛋白的含量CS组显著低于CF组，结论：无龋组菌斑液蛋白受全唾液影响较大。3种液体中总蛋白含量均与患龋无关，但菌斑液和全唾液中某些蛋白可能有重要的抗龋作用。     

乳牙牙菌斑脂肪酸的分析研究

目的 了解乳牙菌斑中脂肪酸组成、含量及其与龋病的关系。方法 43名4-6岁幼儿按龋蚀状况分组，气相色谱-质谱联用技术、气相色谱技术测定乳牙菌斑中脂肪酸组成厦含量，将脂肪酸含量与患龋状况作相关性分析。结果乳牙菌斑含有9种直链饱和脂肪酸及3种直链非饱和脂肪酸。总脂肪酸含量在无龋组、高危组间差别有统计学意义（P〈0．05），C16：0脂肪酸含量在有龋组、无龋组间及无龋组、高危组间差别有统计学意义（P〈0．05）。结论 乳牙菌斑总脂肪酸、C16．0脂肪酸含量所示与乳牙龋病之关系可进一步探讨。