口腔扁平苔藓病损中郎格罕氏细胞与T细胞的关系

本文应用免疫组织化学技术对口腔扁平苔藓病损中的郎格罕氏细胞数量，分布情况及与Ｔ细胞的关系进行了研究，结果显示口腔扁平病损中郎格罕氏细胞周围有ＨＬＡ－ＤＲ阳性的Ｔ细胞及角朊细胞，并且发现郎格罕氏细胞与Ｔ细胞有接触关系。作者认为郎格罕氏细胞与Ｔ细胞之间的密切接触在递呈抗原及启动局部免疫反应过程中起重要作用。     

郎格罕氏细胞:病理状态下的结构、功能及作用

郎格罕氏细胞(LCs)是来源于骨髓的树突状细胞。在很多复层鳞状上皮,如口腔粘膜包括牙龈上皮皆有郎格罕氏细胞存在,位于基底层之上。在诱导免疫时,郎格罕氏细胞起抗原提呈细胞(antigen-presenting cells,APC)的作用,即在正常及病理状态下具有与抗原反应的作用。 郎格罕氏细胞结构 郎格罕氏细胞属于树突状细胞系统一类。它主要来源于骨髓并迁移到上皮中。目前对郎格罕氏细胞的染色主要靠组织化学及免疫化学方法,如确定ADP酶     

下唇疣状癌中朗格罕氏细胞免疫组化观察

本研究采用 S—100蛋白特异标记朗格罕氏细胞(Lc),以 PAP 免疫组化法对16例下唇疣状癌病例的癌上皮及间质内 Lc 分布特点进行了观察,并计算 Lc 出现的频率。结果显示:(1)癌上皮和结缔组织中 Lc 均数增多;(2)间质内 Lc 与淋巴细胞相伴浸润,形成小簇,关系密切;(3)Lc 可能为抗原递呈细胞,在疣状癌免疫反应起动和调节中起着关键作用。     

天疱疮与良性粘膜类天疱疮中郎格罕细胞的免疫组织化学观察

本文采用抗T_6抗体及S—100蛋白免疫组织化学方法,对20例口腔粘膜病的病损组织中的郎格罕细胞进行了观察,初步探讨了其在疱性病损发病中的作用。观察结果表明,天疱疮与良性粘膜类天疱疮上皮组织中的郎格罕细胞均比对比观察组中的扁平苔藓与慢性盘状红斑狠疮明显减少,甚至缺如。本研究提示了郎格罕细胞数量降低,与疱性疾患中的上皮内疱形成或上皮下疱形成、存在有内在的联系。     

成人慢性牙周炎龈上皮中郎格罕氏细胞的观察

本文用 S-100蛋白和溶菌酶抗体的免疫过氧化物酶技术,对10例正常人和20例成人慢性牙周炎龈上皮中郎格罕氏细胞进行鉴定和计数观察。结果表明:牙周炎时龈上皮中郎格罕氏细胞的数目明显高于正常龈上皮(P<0.01),且随炎症细胞浸润程度的加重和菌斑指数记分值的升高而增多,呈显著正相关(P<0.05)。     

牙源性角化囊肿中的郎格罕细胞

用抗S—100蛋白抗体对37例牙源性角化囊肿中的郎格罕细胞定位研究表明,郞格罕细胞主要出现在上皮下方有炎症的上皮内及炎症细胞中,郎格罕细胞的数量在炎症轻时较少,炎症重时较多。本研究结果提示,郎格罕细胞可能来自上皮下的炎症细胞中,并且与炎症的进展及上皮的破环有关。     

根尖囊肿上皮细胞增殖与郎格罕细胞分布的关系

目的 分析根尖囊肿衬里上皮细胞增殖及其与郎格罕细胞分布的关系。方法 收集23例经组织病理学确诊的根尖囊肿石蜡标本，分别进行增殖细胞标记Ki-67和郎格罕细胞标记S-100的免疫组化染色，观察根尖囊肿衬里上皮的细胞增殖及其与郎格罕细胞分布的关系。结果 衬里上皮内的Ki-67阳性的细胞数目与结缔组织囊壁内的S-100阳性的细胞数目呈正相关（P〈0．05），但与衬里上皮内的S-100阳性的细胞数目无相关关系（P〉0．05）。结论 结缔组织囊壁中的郎格罕细胞数目与衬里上皮的增殖活性呈正相关，提示囊壁内由郎格罕细胞参与介导的免疫反应可能有促进上皮增殖的作用。     

口腔鳞状细胞癌纤维连接蛋白的免疫组化研究

目的研究FN与不同分化程度口腔鳞状细胞癌的关系。方法应用用SPTM免疫组化技术检测了30例口腔粘膜鳞状细胞癌（SCC）和10例正常口腔粘膜纤维连接蛋白（FN）在粘膜基底膜及间质的表达。结果正常口腔粘膜FN的基底膜染色密度低，呈连续线状，间质中染色密度高，呈疏松网状。鳞状细胞癌随肿瘤分化程度的降低，基低膜FN的染色密度升高，间质中FN的染色密度与正常相似。基底膜的染色密度与肿瘤分化程度直接相关，基底膜的连续性与肿瘤的分化程度呈负相关，系数是－0．86。结论FN的表达异常与肿瘤分化、浸润和转移有密切关系。     

血管细胞粘附分子-1在口腔鳞状细胞癌中的表达及其与血管生成的关系

目的 观察口腔鳞状细胞癌组织中血管细胞粘附分子-1（vascular cell adhesion molecule-1，VCAM-1）的表达与定位及其与微血管密度（microvessel density，MVD）间的关系，探讨VCAM-1在口腔鳞状细胞癌血管生成中的意义。方法 用免疫组化二步法检测48例口腔鳞状细胞癌组织和10例正常口腔粘膜组织中VCAM-1和CD31表达并计数微血管密度（MVD）。结果 VCAM-1蛋白主要定位于肿瘤细胞的胞膜和胞浆以及血管内皮细胞；VCAM-1在口腔鳞状细胞癌组织中的表达率显著高于正常口腔粘膜组织（P〈0．01）；口腔鳞状细胞癌组织的MVD值显著高于正常口腔粘膜组织（P〈0．01），MVD值与肿瘤浸润深度和有无淋巴结转移密切相关（P〈0．01）；VCAM-1表达阳性组MVD值显著高于VCAM-1表达阴性组（P〈0．01），且VCAM-1表达与MVD呈正相关（P〈0．01）。结论 VCAM-1在口腔鳞状细胞癌组织中的高表达可能在肿瘤的浸润和转移中起重要作用，并与肿瘤血管生成有关。     

PTEN和VEGF在口腔鳞状细胞癌发生发展过程中的表达及意义

目的　探讨抑癌基因PTEN和血管内皮生长因子 (VEGF)在口腔鳞状细胞癌 (oralsquamouscellcancer,OSCC)发生发展不同阶段中的蛋白表达及意义。方法　应用免疫组化S -P法检测 10例正常口腔粘膜、10例上皮单纯增生、15例上皮异常增生及 32例口腔鳞状细胞癌组织中PTEN和VEGF的蛋白表达情况 ,比较这两种蛋白表达之间的关系。结果　PTEN蛋白在正常口腔粘膜、上皮单纯增生、上皮异常增生、和口腔鳞状细胞癌组织中阳性表达率分别为 10 0 % (10 / 10 )、10 0 % (10 / 10 )、93.3% (14 / 15 )和 71.9% (2 3/ 32 ) ;口腔鳞状细胞癌组织中PTEN蛋白的阳性表达率显著低于正常口腔粘膜、上皮单纯增生、上皮异常增生组织 (P <0 .0 5 ) ;高、中、低分化口腔鳞状细胞癌组织中PTEN蛋白的阳性表达率分别为 85 .7%、75 %和 33.3% ,统计学分析表明PTEN在OSCC组织中的蛋白表达与组织分化程度明显相关 (P <0 .0 5 )。PTEN蛋白与VEGF蛋白表达呈负相关趋势 ,但差异无显著性 (P >0 .0 5 )。结论　PTEN蛋白在口腔鳞状细胞癌发生发展过程中的表达呈进行性下调或缺失 ,可能是通过降低细胞粘附、促进血管形成等途径参与口腔鳞状细胞癌的发生和演进过程     

Odontogenic ghost cell tumour with clear cell components: clear cell odontogenic ghost cell tumour?

A case of odontogenic ghost cell tumour (OGCT) with clear cell components was encountered in the mandible of a 63-year-old man. The tumour revealed ameloblastomatous-type epithelial components accompanied by clusters of ghost cells and dentinoid juxtaposed to the odontogenic epithelium. In addition, some areas of the tumour tissue showed sheets and islands of clear, glycogen containing epithelial cells, which were separated by a thin fibrous connective tissue stroma. Both ameloblastic and clear cells exhibited positive immunoreactivities for cytokeratin 19 and AE1/3. It is not known whether this tumour represents a clear cell change of a pre-existing OGCT or a separate and distinct neoplasm derived de novo from the odontogenic epithelium. This tumour was given the term 'clear cell OGCT' because it captures the clear cell components, which is one of the most prominent distinguishing features of the tumour.     

The human periodontal ligament cell: a fibroblast-like cell acting as an immune cell

Jönsson D, Nebel D, Bratthall G, Nilsson B‐O. The human periodontal ligament cell: a fibroblast‐like cell acting as an immune cell. J Periodont Res 2011; 46: 153–157. © 2010 John Wiley & Sons A/S Background: Periodontal ligament cells are fibroblast‐like cells characterized by collagen production but also possessing some osteoblastic features. In the light of numerous studies presented during recent times, which show that human periodontal ligament cells also produce cytokines and chemokines in response to inflammation promoters, it is reasonable to suggest that periodontal ligament cells play a role as promoters of periodontal inflammation through these mechanisms. Material and Methods: The periodontal ligament, which harbours the periodontal ligament cells, is a part of the attachment apparatus comprised of periodontal ligament cells, extracellular matrix and fibres, attaching the root cement to the alveolar bone. Periodontal ligament cells are in close proximity to bacteria within the plaque and the pocket, and thus these cells are readily accessible to bacterial endotoxins and other promoters of inflammation. Results: Cytokines and chemokines, released by periodontal ligament cells upon stimulation with inflammation promoters, reach the blood vessels easily thanks to rich vascularization of the periodontium stimulating recruitment of white blood cells to the site of inflammation. In addition to classical inflammatory cells, such as leucocytes, macrophages and mast cells, the periodontal ligament cells also contribute to periodontal inflammation via their production and release of cytokines and chemokines. Conclusion: Therefore, pharmacological treatment of periodontitis should aim to reduce the release of proinflammatory agents not only from classical inflammatory cells but also from periodontal ligament cells.     

Stem cell patterns in cell lines derived from head and neck squamous cell carcinoma

The initiation, growth, recurrence and metastasis of solid tumours, including squamous cell carcinoma of the head and neck region, have been related to the behaviour of a small subpopulation of 'tumour-initiating' cells. Cells with stem cell characteristics have also been identified in cell lines derived from cancers and the aim of the present work was to extend examination of such cells. Established cell lines were examined for their patterns of colony morphologies and staining, the presence of a Hoechst dye-excluding 'side population', expression of the putative stem cell markers CD44, CD133 and CD29, and their ability to grow as 'cancer spheroids'. Two cell lines, CaLH2 and CaLH3, recently generated from HNSCC tumour biopsies, were similarly examined. All cell lines showed a holoclone/meroclone/paraclone series of colony morphologies and cell sorting indicated that CD44 marker expression was related to clonogenicity. FACS analysis after exposure to Hoechst dye indicated that the CA1, H357 and UK1 cell lines contain a dye-excluding 'side population', a property associated with stem-like subpopulations. When held in suspension, all cell lines formed spheroids that could be re-passaged. These observations indicate that cell lines derived from HNSCC contain cells with stem cell properties and that such cell lines may provide experimental systems relevant to the behaviour of stem cells present in the tumours of origin and to their responses to therapy.     

人口腔鳞状细胞癌细胞系TSCC2016的建立及生物学特性分析

目的: 建立一株口腔鳞状细胞癌细胞系TSCC2016,明确其生物学特性,为研究口腔鳞状细胞癌的发病机制和临床治疗提供工具。方法: 选取新鲜的口腔鳞状细胞癌患者手术标本,采用组织块培养法,建立口腔鳞状细胞癌细胞系TSCC2016,观察其细胞形态和生长特性,对其表面标记、细胞核型和裸鼠成瘤等进行检测。采用SPSS13.0软件包对数据进行统计学分析。结果: TSCC2016的传代已经超过100代。细胞生长稳定,形态为均一的多角形上皮细胞,具有典型的口腔鳞状细胞癌细胞特点。细胞STR分型结果表明,TSCC2016细胞为原代培养出的口腔鳞状细胞癌细胞,无其他肿瘤细胞系污染。TSCC2016细胞的成瘤性较好,成瘤率为100%,瘤体生长较快。结论: 成功建立了一株口腔鳞状细胞癌细胞系TSCC2016,为口腔鳞状细胞癌的基础研究提供了一个稳定的细胞株。     

Cancer stem cell immunophenotypes in oral squamous cell carcinoma

J Oral Pathol Med (2011) 40 : 135–142 Background: The presence of cancer stem cell (CSC) antigens can be evidenced in some human tumors by phenotypic analysis through immunostaining. This study aims to identify a putative CSC immunophenotype in oral squamous cell carcinoma (OSCC) and determine its influence on prognosis. Methods: The following data were retrieved from 157 patents: age, gender, primary anatomic site, smoking and alcohol intake, recurrence, metastases, histologic classification, treatment, disease‐free survival (DFS), and overall survival (OS). An immunohistochemical study for CD44 and CD24 was performed in a tissue microarray of 157 paraffin blocks of OSCCs. Results: In univariate analysis, the immunostaining pattern showed significant influences in relation to OS for alcohol intake and treatment, as well as for the CD44⁺ and CD44^?/CD24^? immunophenotypes. The multivariate test confirmed these associations. Conclusions: Based on our results, the CD44 immunostaining and the absence of immunoexpression of these two investigated markers can be used in combination with other clinicopathologic information to improve the assessment of prognosis in OSCC.     

Squamous cell carcinoma antigen in oral squamous cell carcinomas

Squamous cell carcinoma (SCC) antigen is a tumor-associated antigen isolated from the squamous cell carcinoma of the uterine cervix. In order to estimate the usefulness of the SCC antigen in monitoring the clinical behaviors of oral squamous cell carcinomas, we analyzed clinicopathologically and immunohistochemically 54 cases of squamous cell carcinoma of the oral cavity. Elevated serum SCC antigen levels were detected in 23 (42.6%) out of 54 oral squamous cell carcinomas. The positive rate of serum SCC antigen levels was significantly higher in the patients with advanced clinical stages and poorly differentiated carcinoma. The serum levels declined rapidly after the surgical operation. It is considered that the serum SCC antigen levels could be useful in monitoring the extension, effectiveness of therapy, recurrence and metastases of the oral squamous cell carcinomas. Immunohistochemically, strong staining was seen in the cytoplasm of the well-differentiated carcinoma cells.     

B7-H3在口腔鳞癌细胞中的表达及其意义

目的：研究B7-H3在口腔鳞癌组织和正常口腔黏膜组织中的表达差异,以及B7-H3对口腔鳞癌细胞生物学的影响。方法：RT-qPCR、免疫组化检测B7-H3在口腔鳞癌组织和正常口腔黏膜组织的表达差异;构建B7-H3腺病毒表达载体,感染人舌鳞癌细胞Tca8113,CCK-8、PI染色流式细胞仪检测B7-H3高表达对Tca8113细胞增殖和细胞周期的影响。采用SPSS11.0软件包对数据进行统计学处理。结果：RT-qPCR结果显示,口腔鳞癌组织B7-H3 mRNA表达为3.021±0.2310,显著高于正常口腔黏膜组织（0.6002±0.1010）;与对照组比较,10、50、100感染复数组在作用24h呈现促进细胞增殖作用,S期细胞比例显著增加,72h时增殖作用最强（P〈0.05）;与感染复数1组比较,同时间段50、100组促细胞增殖作用和S期细胞比例显著增加（P〈0.05）,50与100组比较无显著差异（P〉0.05）。结论：口腔鳞癌组织B7-H3mRNA和蛋白表达显著高于正常口腔黏膜组织;B7-H3高表达,可促进口腔鳞癌细胞增殖。     

舌癌单细胞培养建系与癌干细胞相关标志的检测

目的 以舌癌Tca8113M1细胞系单细胞培养建系为基础,观察Tca8113M1细胞系中舌癌干细胞存在的现象及其相关标志的变化规律。方法 选取Tca8113M1细胞系,以有限稀释法进行体外单细胞培养并建立细胞亚系,在证实其成瘤性的基础上,进一步采用流式细胞术检测癌干细胞相关标志CD44、CD184、细胞外可溶性抗原（ESA）的表达情况,着重观察单个细胞培养形成细胞克隆的形态与时间。结果 以有限稀释法获取192个Tca8113M1舌癌单个细胞,在96孔板中进行体外培养,获取12个细胞亚系（获取比例为6.25%）,均有高成瘤性。癌干细胞相关标志CD44 与ESA均为高水平表达,而CD184表达则在12个细胞系之间有差异。在单个细胞培养中,形成完全克隆、部分克隆与旁克隆3种形态,12个细胞亚系均源于单个细胞形成的完全克隆,均可进行连续传代与扩增,而部分克隆与旁克隆则在后续培养中逐渐衰老与消亡。结论 Tca8113M1细胞系中可能存在癌干细胞,而单细胞培养可形成完全克隆并建立细胞亚系,是进行舌癌干细胞后续研究重要的细胞培养模式。