转化生长因子β1基因多态性及其血清水平与儿童哮喘易感性的关系

目的检测转化生长因子β1(TGF-β1)基因C-509T、T869C位点多态性在哮喘儿童中的分布频率,并探讨其与哮喘易感性及临床表型的相关性。方法以110例哮喘患儿(哮喘组)和144例非哮喘患儿(对照组)为研究对象,应用聚合酶链反应-限制性片段长度多态性分析(PCR-RFLP)技术对C-509T、T869C单核苷酸多态性进行基因分型,同时用ELISA法检测血清TGF-β1水平。结果哮喘组C-509T位点T等位基因频率显著高于对照组(P=0.006,OR=1.66,95%CI:1.16～2.37),哮喘组CC基因型频率低于对照组(P<0.05),CC基因型携带者患哮喘病的危险性明显降低(P=0.004,OR=0.46,95%CI:0.27～0.79)。TGF-β1基因T869C位点基因型及等位基因频率在哮喘组和对照组间分布差异均无统计学意义(P>0.05);哮喘组患儿血清TGF-β1水平显著高于对照组(P<0.01),C-509T位点携带T等位基因患儿血清TGF-β1水平高于非携带者(P<0.05)。结论 TGF-β1基因C-509T位点T等位基因可能是河南儿童哮喘易感基因,T869C位点多态性可能与儿童哮喘发病无相关性。     

TGF-β1基因多态性与乙型肝炎肝硬化的相关性研究

目的探讨转化生长因子-β1（TGF-β1）基因多态性是否与慢性HBV感染人群肝硬化发生的遗传易感相关。方法收集173例中国汉族慢性HBV感染患者（肝硬化组42例和非肝硬化组131例）和52例健康对照者的外周血标本,提取白细胞DNA,基因直接测序法检测TGF-β1基因4个多态性（869T/C、915G/C、-800 G/A、-509 C/T）,ELISA法检测TGF-β1血清浓度,比较各组TGF-β1基因型分布频率、等位基因频率及TGF-β1血清水平的差异。结果 （1）乙型肝炎肝硬化组TGF-β1869位点以TT基因型为主（47.6%）,高于非肝硬化组（25.2%）和对照组（19.2%）,差异有统计学意义（P〈0.05）;869T等位基因频率（63.1%）明显高于正常对照组（44.2%）,差异有统计学意义（P〈0.05）,乙型肝炎肝硬化组与慢性HBV感染非肝硬化组（53.8%）、慢性HBV感染非肝硬化组与正常组869T等位基因携带率相比,差异无统计学意义（P〉0.05）。（2）所检其余多态性位点基因型及基因频率未见组间统计学差异。（3）869T/C不同基因型患者的血清TGF-β1水平不一,T/T型携带者较T/C和C/C型可见明显增高（P〈0.017）。结论 TGF-β1869T可能与汉族人群乙型肝炎肝硬化的遗传易感相关,其机制可能与该多态性对TGF-β1血清水平的影响有关。     

TGF-β1基因多态性与乙型肝炎肝硬化的相关性研究

目的探讨转化生长因子-β1(TGF-β1)基因多态性是否与慢性HBV感染人群肝硬化发生的遗传易感相关。方法收集173例中国汉族慢性HBV感染患者(肝硬化组42例和非肝硬化组131例)和52例健康对照者的外周血标本,提取白细胞DNA,基因直接测序法检测TGF-β1基因4个多态性(869T/C、915G/C、-800 G/A、-509 C/T),ELISA法检测TGF-β1血清浓度,比较各组TGF-β1基因型分布频率、等位基因频率及TGF-β1血清水平的差异。结果 (1)乙型肝炎肝硬化组TGF-β1869位点以TT基因型为主(47.6%),高于非肝硬化组(25.2%)和对照组(19.2%),差异有统计学意义(P<0.05);869T等位基因频率(63.1%)明显高于正常对照组(44.2%),差异有统计学意义(P<0.05),乙型肝炎肝硬化组与慢性HBV感染非肝硬化组(53.8%)、慢性HBV感染非肝硬化组与正常组869T等位基因携带率相比,差异无统计学意义(P>0.05)。(2)所检其余多态性位点基因型及基因频率未见组间统计学差异。(3)869T/C不同基因型患者的血清TGF-β1水平不一,T/T型携带者较T/C和C/C型可见明显增高(P<0.017)。结论 TGF-β1869T可能与汉族人群乙型肝炎肝硬化的遗传易感相关,其机制可能与该多态性对TGF-β1血清水平的影响有关。     

转化生长因子-β基因多态性与矽肺相关性研究

①目的探讨转化生长因子-β（TGF-β）基因多态性与矽肺的关系。②方法采用1：1配对的病例对照研究方法,矽肺组为确诊的77例Ⅰ期矽肺患者,对照组与矽肺纽同性别、同民族、同一工作车间或相同采掘工作面,年龄差〈5岁,开始接尘时间和累积接尘工龄差〈不超过2年的接尘工人。应用聚合酶链反应一限制性片段长度多态性（PCR—RFLP）技术对TGF-β启动区的-509C／T住点和第一外显子的＋869T／C、＋915G／C住点的基因多态性进行检测。⑧结果矽肺组TGF-β启动区的-509C／T住点等住基因和基因型频率与对照组比较,差异均有显著性（P〈0．05）;＋869T／C位点等住基因和基因型频率与对照纽比较,差异均无显著性（P〉0．05）;＋915G／C位点等位基因和基因型频率与对照组比较,差异均有显著性（P〈0．05）。④结论TGF-β—509TT和＋915GC基因型是矽肺的易感基因型;同时携带TGF-β—509＊T和TGF-β＋915＊C等位基因的接尘人群,更易感矽肺。未发现TGF-β＋869位点基因多态性与矽肺易感性有关联。     

转化生长因子-β1基因单核苷酸多态性与卵巢癌的相关性

目的研究转化生长因子β1(TGF-β1)基因启动子多态性各等位基因及基因型在卵巢癌患者中的分布频率,初步分析其基因型及血清水平与卵巢癌的相关性。方法采用聚合酶链反应-限制性片段长度多态性技术,检测110例卵巢癌患者和120例正常对照组TGF-β1的基因多态性,包括TGF-β1基因启动子-800G/A,-509C/T位点,同时采用酶联免疫吸附试验检测血清TGF-β1水平。结果卵巢癌患者血清TGF-β1水平显著高于对照组(P<0.01),TGF-β1基因-800G/A位点多态性在卵巢癌组和正常人群中的分布差异无显著性(P>0.05),而TGF-β1基因-509C/T多态性各等位基因及基因型频率在两组人群中的分布差异有显著性(P<0.05),等位基因频率的相对风险分析发现,T等位基因携带者患卵巢癌的风险是C等位基因的1.702倍,携带T等位基因的卵巢癌患者血清TGF-β1水平显著高于不携带者(P<0.01)。结论TGF-β1基因-509C/T多态性与卵巢癌的发病具有相关性,其中T等位基因可能是卵巢癌发病的遗传易感基因;携带T等位基因的个体可能通过促进TGF-β的高度表达进而增加了卵巢癌的发病风险。     

TGF-β_1基因多态性与妊娠期高血压疾病的相关性研究

目的：研究TGF-β1基因（转化因子-β1）启动子序列509C/T的多态性与妊娠期高血压病（妊高征）的关系。方法：应用聚合酶链反应-限制性片段长度多态性技术检测我院2010年1～12月100例妊高征患者与100例正常妊娠妇女的TGF-β1基因多态性,比较两组人群TGF-β1基因型和等位基因分布频率是否存在统计学差异。结果：在妊高征组患者中CC基因型占48%、CT基因型占31%、TT基因型占69%;在正常孕妇中CC基因型占15%、CT基因型占10%、TT基因型占27%。两组的基因型及等位基因分布差异有统计学意义（P〈0.05）。结论：TGFβ1-509 C/T基因多态性与妊娠高血压综合征的发病有明显相关性。     

宁夏回、汉族群体TGF-β1启动子区基因多态性研究

目的探讨转化生长因子β1(Transforming Growth Factor-β1,TGF-β1)基因启动子区SNP-800(G/A)和SNP-509(C/T)多态性在宁夏回、汉族人群中的分布特征。方法应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术对210例回族和308例汉族人群转化生长因子β1启动子区(-800位点、-509位点)两个多态性位点进行检测。结果宁夏回族群体TGF-β1基因两个多态性位点各基因型频率及等位基因频率分别为:-800(GG:97.62%;GA:2.38%;AA:0%;G:98.81%;A:1.19%),-509(CC:21.90%;CT:56.67%;TT:21.43%;C:50.24%;T:49.76%);宁夏汉族群体TGF-β1基因两个多态性位点各基因型频率及等位基因频率分别为:-800(GG:99.03%;GA:0.97%;AA:0%;G:99.52%;A:0.48%);-509(CC:27.27%;CT:50.97%;TT:21.75%;C:52.76%;T:47.24%),两组间差异均无统计学意义(P>0.05)。按性别分组,两个多态性位点的基因型频率及等位基因频率差异亦无统计学意义(P>0.05)。结论 TGF-β1基因启动子区-800、-509两个多态性位点在宁夏回、汉族人群及不同性别间差异无统计学意义。     

壮族矽肺患者外周血转化生长因子-β1基因T+869C、G+915C位点多态性分布状况

目的探讨壮族矽肺患者转化生长因子-β1(TGF-β1)T+869C、G+915C位点多态性分布状况。方法随机选择汉族、壮族健康未接触矽尘工人各30例,壮族接触矽尘工人42例,汉族接触矽尘工人43例,壮族矽肺患者40例,汉族矽肺患者30例。用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法检测TGF-β1T+869C、G+915C位点多态性。结果各组T+869C位点上TT、TC、CC 3种基因型构成比比较差异无统计学意义(P均>0.05);各组G+915C位点基因型均为GG。结论尽管TGF-β1可能参与了矽肺的发生、转归过程,但汉族、壮族人员,TGF-β1的这种功能似乎与其基因上的T+869C、G+915C位点上的突变无关。     

转化生长因子β1基因多态性与广西壮族人原发性高血压的遗传易感性

目的: 研究转化生长因子β1(TGF-β1)基因第1外显子+869T/C及+915G/C多态性与广西壮族人原发性高血压的关系.方法: 采用序列特异性引物聚合酶链反应(PCR-SSP)技术,检测172例原发性高血压患者和180 例健康对照者转化生长因子β1的基因多态性.结果: TGF-β1基因+869T/C多态性在两组人群中的分布差异有统计学意义(P<0.05),等位基因频率的相对风险分析发现, C等位基因携带者患原发性高血压的风险是T等位基因的1.525倍(OR=1.525,95%CI:1.133～2.053),携带C等位基因的原发性高血压患者收缩压水平显著高于不携带者(22.0±3.9 kPa vs 21.1±3.1 kPa,P<0.05);而TGF-β1基因+915G/C位点多态性在原发性高血压组和正常人群中的分布差异无统计学意义.结论: TGF-β1基因+869T/C多态性与广西壮族人原发性高血压的发病具有相关性,其中C等位基因可能是原发性高血压发病的遗传易感基因;携带C等位基因的个体可能通过促进收缩压的升高进而增加了原发性高血压的发病风险.     

煤工尘肺患者血清转化生长因子β1水平与其基因多态性的关系研究

目的探讨煤工尘肺患者血清转化生长因子1β(TGF-1β)与其-509位点基因多态性的关系。方法选择101例汉族煤工尘肺患者,采集外周静脉血,应用TGF-1βEL ISA试剂盒检测其血清TGF-1β,应用PCR-RFLP技术检测其TGF-1β基因-509位点基因型。结果血清TGF-1β在T/T突变型、C/T杂合型和C/C野生型三组煤工尘肺患者中分别为(90.029±17.774)ng/m l、(60.988±26.475)ng/m l、(55.960±28.833)ng/m l,差异有统计学意义(P<0.05);不同年龄、不同工龄、不同期别煤工尘肺患者间的差异无统计学意义(P>0.05)。结论煤工尘肺患者血清TGF-1β水平可能与TGF-1β基因-509位点多态性有关,尚未观察到不同年龄、工龄、期别的煤工尘肺患者血清TGF-1β水平差异有统计学意义。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.