共查询到20条相似文献,搜索用时 12 毫秒
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Jinmi Lee Seok-Woo Hong Se Eun Park Eun-Jung Rhee Cheol-Young Park Ki-Won Oh Sung-Woo Park Won-Young Lee 《Metabolism: clinical and experimental》2014
Objective
Hepatokine fibroblast growth factor (FGF) 21 takes part in the regulation of lipid metabolism in the liver and adipose tissue. We investigated whether exendin-4 regulates the expression of FGF21 in the liver, and whether the effects of exendin-4 on the regulation of FGF21 expression are mediated via silent mating type information regulation 2 homolog (SIRT) 1 or SIRT6.Materials/methods
The C57BL/6J mice were fed a low fat diet, high fat diet, or high fat diet with 1 nmol/kg/day exendin-4 intraperitoneal injection for 10 weeks. HepG2 used in vitro study was treated with palmitic aicd (0.4 mM) with or without exendin-4 (100 nM) and FGF21 (50 nM) for 24 hours. The change of FGF21 and its receptors expression by exendin-4 were measured using quantitative real-time RT-PCR and Western blot. The intracellular lipid content in HepG2 cells was evaluated by Oil Red O staining. Inhibition of FGF21, SIRT1 and SIRT6, by 10 nM siRNA was performed to establish the signaling pathway of exendin-4 action in hepatic lipid metabolism.Results
Exendin-4 increased the expression of FGF21 and its receptors in high fat diet-induced obese mice. In addition, recombinant FGF21 treatment reduced lipid content in palmitic acid-treated HepG2 cells. We also observed significantly decreased expression of peroxisomal proliferator-activated receptor (PPAR) α and medium-chain acyl-coenzyme A dehydrogenase (MCAD) in hepatocytes transfected with FGF21 siRNA. In cells treated with exendin-4, inhibition of SIRT1, but not SIRT6, by siRNA significantly repressed the expression of FGF21 mRNA, whereas decreased SIRT1 expression by inhibition of FGF21 was not observed.Conclusions
These data suggest that exendin-4 could improve fatty liver by increasing SIRT1-mediated FGF21. 相似文献4.
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Zhong Q. Wang Xian H. Zhang Yongmei Yu Russell C. Tipton Ilya Raskin David Ribnicky William Johnson William T. Cefalu 《Metabolism: clinical and experimental》2013
Objective
Nonalcoholic fatty liver disease (NAFLD) is a common liver disease which has no standard treatment. In this regard, we sought to evaluate the effects of extracts of Artemisia santolinaefolia (SANT) and Artemisia scoparia (SCO) on hepatic lipid deposition and cellular signaling in a diet-induced obesity (DIO) animal model.Materials/Methods
DIO C57/B6J mice were randomly divided into three groups, i.e. HFD, SANT and SCO. Both extracts were incorporated into HFD at a concentration of 0.5% (w/w). Fasting plasma glucose, insulin, adiponectin, and FGF21 concentrations were measured.Results
At the end of the 4-week intervention, liver tissues were collected for analysis of insulin, AMPK, and FGF21 signaling. SANT and SCO supplementation significantly increased plasma adiponectin levels when compared with the HFD mice (P < 0.001). Fasting insulin levels were significantly lower in the SCO than HFD mice, but not in SANT group. Hepatic H&E staining showed fewer lipid droplets in the SCO group than in the other two groups. Cellular signaling data demonstrated that SCO significantly increased liver IRS-2 content, phosphorylation of IRS-1, IR β, Akt1 and Akt2, AMPK α1 and AMPK activity and significantly reduced PTP 1B abundance when compared with the HFD group. SCO also significantly decreased fatty acid synthase (FAS), HMG-CoA Reductase (HMGR), and Sterol regulatory element-binding protein 1c (SREBP1c), but not Carnitine palmitoyltransferase I (CPT-1) when compared with HFD group. Neither SANT nor SCO significantly altered plasma FGF21 concentrations and liver FGF21 signaling.Conclusion
This study suggests that SCO may attenuate liver lipid accumulation in DIO mice. Contributing mechanisms were postulated to include promotion of adiponectin expression, inhibition of hepatic lipogenesis, and/or enhanced insulin and AMPK signaling independent of FGF21 pathway. 相似文献7.
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Sangiao-Alvarellos S Helmling S Vázquez MJ Klussmann S Cordido F 《Molecular and cellular endocrinology》2011,335(2):177-188
Ghrelin, a hormone whose levels increase during food deprivation, plays a pivotal role in the regulation of food intake, energy metabolism and storage, as well as in insulin sensitivity. Here, we investigated the effects of acyl-ghrelin neutralization with the acyl-ghrelin-binding compound NOX-B11(2) during the fasting-refeeding cycle. Our data demonstrate that ghrelin neutralization with NOX-B11(2) impairs recuperation of lost body weight after food deprivation. Analysis of enzymes involved in glucose and lipid metabolism in liver of fed, fasted and refed rats revealed that neutralization of acyl-ghrelin resulted in minor decreases in the enzymes of glycolytic and lipogenic pathways during fasting. However, during refeeding these enzymes as well as glycogen levels recovered more slowly when acyl-ghrelin was blocked. The high levels of ghrelin in response to food deprivation may contribute to an adequate decrease in hepatic glycolytic and lipogenic enzymes and aid in the recovery of body weight and energetic reserves once food becomes available after the fasting period. 相似文献
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Metformin prevents alcohol-induced liver injury in the mouse: Critical role of plasminogen activator inhibitor-1 总被引:7,自引:0,他引:7
Bergheim I Guo L Davis MA Lambert JC Beier JI Duveau I Luyendyk JP Roth RA Arteel GE 《Gastroenterology》2006,130(7):2099-2112
BACKGROUND & AIMS: The biguanide drug metformin has recently been found to improve steatosis and liver damage in animal models and in humans with nonalcoholic steatohepatitis. METHODS: The aim of the present study was to determine whether metformin also prevents steatosis and liver damage in mouse models of acute and chronic alcohol exposure. RESULTS: Acute ethanol exposure caused a >20-fold increase in hepatic lipids, peaking 12 hours after administration. Metformin treatment significantly blunted the ethanol effect by >60%. Although metformin is a known inducer of AMP kinase (AMPK) activity, the hepatoprotective property of metformin did not correlate with activation of AMPK or of AMPK-dependent pathways. Instead, the protective effects of metformin correlated with complete prevention of the upregulation of plasminogen activator inhibitor (PAI)-1 caused by ethanol. Indeed, a similar protective effect against acute alcohol-induced lipid accumulation was observed in PAI-1-/- mice. Hepatic fat accumulation caused by chronic enteral ethanol feeding was also prevented by metformin or by knocking out PAI-1. Under these conditions, necroinflammatory changes caused by ethanol were also significantly attenuated. CONCLUSIONS: Taken together, these findings suggest a novel mechanism of action for metformin and identify a new role of PAI-1 in hepatic injury caused by ethanol. 相似文献
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The Intestinal Nuclear Receptor Signature With Epithelial Localization Patterns and Expression Modulation in Tumors 总被引:1,自引:0,他引:1
Salvatore Modica Stefania Murzilli Lorena Salvatore Antonio Nicolucci Massimiliano Copetti Serena Veschi Giuseppe Palasciano Johan Auwerx Antonio Moschetta 《Gastroenterology》2010,138(2):636-76
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Chunchun Han Huofu Wan Shuang Ma Dandan Liu Fang He Jiwen Wang Zhixiong Pan Hehe Liu Liang Li Hua He Hongyong Xu Shouhai Wei Feng Xu 《Molecular and cellular endocrinology》2014
Our result showed in the fatty liver formation induced-by overfeeding goose, it was accompanied by an activation of mammalian target of rapamycin (mTOR) pathway and cell proliferation. Recent studies have suggested a crucial role for mammalian sirtuin 1 (SIRT1) in regulating lipid metabolism and cell proliferation, so we hypothesize that resveratrol -activated and nicotinamide -inhibited SIRT1 acts goose hepatocellular lipid metabolism and cell proliferation by mTOR signal pathway. Here we show that both resveratrol and nicotinamide could evidently affect the DNA synthesis rate, the lipids accumulation, the mRNA level and protein content of genes involved in the lipids metabolism, mTOR signal pathway, and the cell cycle progression of goose primary hepatocytes. Moreover, rapamycin decreased the effect of nicotinamide on lipids accumulation and cell proliferation. These findings suggest that SIRT1 functions as a regulator for mTOR signaling and plays an essential role in the regulation of hepatocyte lipid metabolism and cell proliferation. 相似文献
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L.M. Cornall M.L. Mathai D.H. Hryciw A.C. Simcocks P.E. O’Brien J.M. Wentworth A.J. McAinch 《Molecular and cellular endocrinology》2013
Gene knockout and agonist studies indicate that activation of the G protein-coupled receptor, GPR119, protects against diet-induced obesity and insulin resistance. It is not known if GPR119 activation in skeletal muscle mediates these effects. To address this uncertainty, we measured GPR119 expression in skeletal muscle and determined the effects of PSN632408, a GPR119 agonist, on the expression of genes and proteins required for fatty acid and glucose oxidation in cultured myotubes. GPR119 expression was readily detected in rat skeletal muscle and mRNAs were induced by 12 weeks of high-fat feeding. Treatment of cultured mouse C2C12 myotubes with 5 μM PSN632408 or 0.5 mM palmitate reduced expression of mRNAs encoding fatty acid oxidation genes to similar extents. More so, treatment with PSN632408 decreased AMPKα (Thr172 phosphorylation) activity in the absence of palmitate and ACC (Ser79 phosphorylation) activity in the presence of palmitate. In human primary myotubes PSN632408 decreased expression of PDK4 and AMPKα2 mRNA in myotubes derived from obese donors. These data suggest GPR119 activation in skeletal muscle may impair fatty acid and glucose oxidation. 相似文献
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