The presence of complement fixing antibodies to Rous sarcoma virus (Schmidt-Ruppin strain) in patients with various forms of neoplasia.

The presence in men of antibodies to Rous sarcoma virus, an oncogenic virus of avian origin, was demonstrated. The highest antibody incidence was found in patients with various forms of cancer (34.4%, as against the value of 12% recorded in blood donors); these patients also showed an increased antibody level - 1/64 titers were detected in 43 out of 7 patients. The possibility of a human infection with animal oncogenic viruses is discussed, without excluding the hypothesis of a paraimmune reaction.     

Investigations of the presence of serum antibodies to Epstein-Barr virus in patients with various tumors

Serum samples from 150 patients with various malignant or benign tumors and from 20 clinically healthy subjects were tested by indirect immunofluorescence (IF) reaction for the presence of specific antibodies to Epstein-Barr-virus-determined viral capsid antigen (EB VCA). The IF test-carried out in multitest-slide EB3 and Raji cell preparations-revealed antibodies to EB VCA in 102 of the patients with tumors and in 4 of the healthy persons.     

Investigations on the presence of antibodies to papova viruses in patients with different forms of cancer and in other categories of patients or apparently healthy subjects. Note I. Neutralizing antibodies to SV-40 in the cerebrospinal fluid of patients with brain tumors and chronic neurological diseases

Neutralizing antibodies to SV-40 were detected in cerebrospinal fluid samples from 16 out of 43 patients with brain tumors and in 6 out of 20 patients with different nonmalignant chronic neurological diseases. The patients' age excluded the correlation with a vaccine-associated or occupational contact with SV-40. The results ascertain the occurrence of SV-40 or of an antigenically related virus in the population of Romania.     

Effect of phenazepam in various medicinal forms on psychophysical state of patients with anxiety disorders

Differences between phenazepam formulations were found in the clinical study of drug effects on the psychophysiological state in patients with anxiety disorder. Phenazepam did not cause negative changes of psychophysiological parameters. In patients with anxiety disorders, the most significant positive therapeutic actionon psychophysiological state was observed in the case of using transdermal phenazepam formulation.     

Epstein-Barr virus (EBV). X. Incidence of EBV antibodies in patients with malignant tumors

Serum samples from 31 patients with various types of malignancies, 18 patients with different viral infections and 6 healthy subjects as controls, were tested by indirect immunofluorescence (IF) method for antibodies against viral capsid antigens (VCA) and the presence of active EBV infection. EBV antibodies anti-VCA were detected in 19 patients with tumors, in 8 patients with viral infections and in 2 healthy subjects. EBV active infection was found out in 9/19, 3/8 and 0/2 EBV anti-VCA positive patients with malignancies, different viral infections and healthy subjects respectively.     

Epstein-Barr virus (EBV). III. Incidence of EBV antibodies in patients with various tumors

Serum samples from 553 patients with various tumors, from 26 patients with different viral infections and from 78 clinically healthy subjects were tested by indirect immunofluorescence (IF) reaction for the presence of specific antibodies to Epstein-Barr virus. The test revealed antibodies to EBV in 127 patients with tumors, in 14 patients with viral infections and in 8 healthy persons.     

Epstein-Barr virus (EBV). VI. Incidence of EBV antibodies in patients with malignant lymphoproliferative diseases

Serum samples from 52 patients with malignant lymphoproliferative diseases and 12 clinically healthy subjects were tested by indirect immunofluorescence (IF) reaction for the presence of specific EB antibodies anti-VCA and active EBV infection. The tests revealed EB antibodies anti-VCA in 32 patients with lymphoproliferative diseases and in 2 clinically healthy subjects and active EBV infection in 18/32 and in 0/2 EB anti-VCA positive patients and clinically healthy subjects, respectively.     

Possible relation between viruses and oromaxillofacial tumors. II. Research on the presence of the SV40 antigen and specific antibodies in patients with oromaxillofacial tumors

SV40 antigen was detected on sections through malignant oromaxillofacial tumors in 47.8% of the cases, with a higher incidence among the epithelium originating tumors. Anti-SV40 CF antibodies were detected in 83.6% of the examined subjects with cancer, but only in 57.9% of the blood donors. The titers were quite low in general. The results are discussed from the point of view of the immunologic status of the patients.     

恶性血液病患者核仁组成区嗜银蛋白表达活性的研究

目的：探讨恶性血液病患者T淋巴细胞核仁组成区相关蛋(Ag-NORs)表达特点及临床价值。方法：利用KL型肿瘤免疫图象分析系统及其配套细胞培养银染试和方法,对125例正常及恶性血液病患者外周血T淋巴细胞进行染色分析。结果：恶性血液病患者rDNA转录活性明显低于正常对照组（P＜0．01）,分组观察慢性粒细胞白血病（CML）,急性非淋巴细胞白血病（ANLL）,非何杰金氏淋巴瘤（NHL）及急性淋巴细胞白血病（ALL）组明显低于正常对照组（P＜0．01）和（P＜0．05）,多发性骨髓瘤（MM）,再生障碍性贫血（AA）组与正常对照组比较,无明显差异（P＞0．05）,未完全缓解组与正常对照组比较有显著差异（P＜0．01）,结论：恶性血液病患者普遍存在T细胞免疫功能降低,T淋巴细胞Ag-NORs检测指标可以灵敏地反映各类恶性血液病患者病情进展程度和机体免疫状况的相互关系,并可作为各类恶性血液病的诊断及病情监测的实验参考指标。     

Studies on the effects of l-ascorbic acid on acetaminophen-induced hepatotoxicity: II. An in vivo assessment in mice of the protection afforded by various dosage forms of ascorbate

The protection provided by various forms of ascorbate, in several dosage regimens, against acetaminophen (APAP)-induced hepatotoxicity was assessed in male MF1 mice. Hepatotoxicity was produced by treatment with 450 mg APAP/kg po and evaluated 24 hr later by the change in liver weight to body weight ratio and from a histological score of liver necrosis. All treatments were given by gavage in 0.5% tragacanth at 20 ml/kg. No significant protection was given by l-ascorbic acid (LAA) at 50, 150, or 300 mg/kg given at the same time as APAP, nor at 150 or 300 mg/kg given 1 hr afterward. A dose of 150 mg LAA/kg at the same time as, and 2 hr after APAP also failed to give protection. In contrast, other dosage forms showed a protective action. Ascorbyl palmitate provided significant protection at 300 mg/kg. After 600 and 900 mg/kg doses, the liver weight/body weight ratio and necrosis scores were not significantly greater than those in the vehicle controls. The degree of protection by a microencapsulated LAA preparation (MEAA) was related to the fate of the microcapsules in the gastrointestinal tract. This finding suggests that the pharmacokinetic profile of LAA, relative to the time scale of the hepatotoxic processes, may be critical. Sodium ascorbate gave no protection when used in combination with MEAA. The results indicate that ascorbic acid in certain biopharmaceutical forms may have potential use in the development of a safer dosage form of APAP.     

Investigations on the presence of papova virus in certain forms of human cancer. Note 2. Brain tumors

Two serially transmissible tumors (metastatic lymphosarcomas) were induced in the hamster by inoculation of human brain tumor material--tissue homogenate from a malignized fibroma or DNA extracted from a meningioma. SV40 V antigen was made evident by indirect immunofluorescence in 4 out of 18 human tumors, while SV40 T antigen could only be demonstrated in the tumors experimentally induced in hamsters. Antibodies to SV40 T antigen were detected in 12 out of 60 patients with different tumors, in 1 out of 40 blood donors and in 7 out of 18 hamsters carrying experimentally induced tumors.