白细胞分类显微镜法与CD1600血细胞分析仪法的对比分析

目的 比较CD1600血细胞分析仪和人工显微镜油镜计数法对白细胞分类计数的差异情况.方法 EDTA-K2抗凝全血标本分别采用仪器法和手工法作白细胞分类计数.结果 仪器法和手工法白细胞分类计数结果当白细胞总数低于15×109/L结果无显著性差异(P＞0.05),但当白细胞总数超过15×109/L时,两者计数有差异.结论 仪器法方便、快捷、重复性好,白细胞总数低于15×109/L时可以代替显微镜油镜计数法,当白细胞数量超过15×109/L,或有不正常细胞出现及细胞分布异常时用显微镜目视计数法更准确.     

全自动血液分析仪对晚期肝硬化腹水检测的应用评价

目的:观察全自动血液分析仪在晚期肝硬化腹水检测中的临床应用价值。方法:应用Sysmex XT-1800i全自动血液分析仪(仪器法)检测晚期肝硬化患者腹水中的白细胞总数、分类和异常细胞,并与手工显微镜法(手工法)进行对比。结果:仪器法与手工法的白细胞计数和分类结果相关性好,相关系数(r)均>0.950(P<0.05);仪器法检测异常细胞的灵敏度为80.00%,特异度为99.12%,Youden指数为0.7912,阳性预测值为91.01%,阴性预测值为97.80%,与手工法结果一致率为0.9512。结论:全自动血液分析仪(Sysmex XT-1800i)能高效、准确地对肝硬化腹水中白细胞计数和肿瘤细胞进行提示。     

ABX micros 60三分群血细胞分析仪白细胞分类与手工镜检法比较

目的探讨全自动血细胞分析仪白细胞分类计数与手工法的方法学差异.方法随机抽取94例普通门诊病人的ED-TA-K2抗凝静脉血进行白细胞分类仪器法与手工镜检法测定,并对所得的两组结果进行比较.结果两种方法中性粒细胞与淋巴细胞计数差异无明显意义(P＞0.05);中间细胞计数差异有显著性意义(P＜0.01),仪器法明显高于手工法.结论ABX micros 60血细胞分析仪与手工法在中间细胞计数方面存在较大的方法学差异,故不能用该仪器完全取代显微镜涂片检查.     

17例有核红细胞存在对白细胞计数干扰的原因分析

日本希森美康最新一代全血细胞检测仪器,是XN系列全自动血细胞分析仪,在日常血细胞分析检验中被广泛应用。过往案例报道,XN系列全自动血细胞分析仪,能通过识别有核红细胞,达到自动纠正白细胞计数的目的,与显微镜手工镜检方法比较,结果无明显偏差,可信度及相关性较好。针对此情况,收集我院2021年1月至9月的17例患者的结果,发现当有核红细胞计数NRBC为25.3个/100WBC及NRBC>61.3个/100WBC,并伴有大量幼稚红细胞或白细胞碎片存在时,仪器自动纠正白细胞计数与显微镜手工镜检分类纠正白细胞计数比较,结果有明显偏差,两者之间存在不符。由此证明先进的全血细胞分析仪仍无法取代显微镜手工镜检法纠正白细胞的地位。因此,当大量有核红细胞存在时,仍需要手工纠正白细胞计数。     

应用全自动血细胞分析仪应该重视血涂片镜检

目的 目的:应用全自动血细胞分析仪应该重视血涂片镜检.方法 采用SySmex XS 800 i全自动五分类血细胞分析仪检测,异常或未出结果 的标本进行人工瑞士染色后涂片镜检.结果 人工血涂片镜检要比血细胞分析仪更能准确地反映出病人血细胞形态的变化.结论 全自动血细胞分析仪虽然快速、准确,但也不能忽视血涂片镜检的重要作用.     

二种血细胞分析仪白细胞分类的应用评价

九 十年代 ,三分类血细胞分析仪的使用 ,减轻了检验工作者的劳动强度 ,提高了检测效率。近年来 ,可以对血液细胞多种组分 (包括白细胞的五种组分 )进行分类的五分类血细胞分析仪 ,其操作更为简便 ,检测速度更快 ,使血细胞分析技术有了极大的提高。但其结果与手工法比较准确性怎样呢 ?本文作者将此二类血细胞分析仪白细胞分类结果与手工法进行比较 ,并作出评价。1　材料与方法1.1　仪器及试剂美国雅培CELL DNY 170 0型血细胞分析仪 ,试剂均由雅培公司生产 ;德国拜耳ADVIA 12 0型血细胞分析仪 ,试剂均由拜耳公司生产 ;OLYMPUS显微镜由…     

UF-100尿流式细胞仪应用于脑脊液细胞计数

目的 建立流式细胞仪计数脑脊液标本细胞的快速、准确、直观的检测方法并验证该方法的可行性.方法 无菌条件下采集患者脑脊液标本,使用UF-100流式细胞仪分别对117例脑脊液标本中红细胞与白细胞数计数,并与采用镜检法检测相同脑脊液标本所得的结果用统计学方法进行比较.结果 流式细胞仪法与镜检法计数脑脊液中的血细胞结果差异无显著性.结论 流式细胞仪计数脑脊液中的血细胞快速、结果准确且重复性好,适合临床常规检测脑脊液标本.     

BC-3000 plus血细胞分析仪在脑脊液细胞计数中的应用

目的寻找一种简便快捷的脑脊液(cerebrospinalfluid,CSF)细胞计数方法。方法采用BC-3000plus血细胞分析仪预稀释模式直接进行CSF计数分析;并与显微镜下使用牛氏细胞计数盘计数结果进行对比。结果与计数盘计数相比,脑脊液白细胞采用BC-3000plus计数具有准确、快速的优点;与计数盘计数结果间有良好的相关性;结论用BC-3000plus血细胞分析仪的预稀释模式进行脑脊液细胞计数快速、准确,不失为一种有效的手段。其临床应用价值值得进一步探讨。     

超敏C-反应蛋白检测对小儿浆膜腔积液性质鉴别的应用

目的探讨超敏C-反应蛋白（hs-CRP）对小儿浆膜腔积液性质鉴别的价值。方法收集2008—2009年本院43例浆膜腔积液标本,使用日立7600全自动生化分析仪检测积液和血清的LDH及蛋白含量,采用免疫荧光双抗体夹心法检测积液中的hs—CRP含量。结果hs-CRP检测对于浆膜腔积液性质鉴别的灵敏度为92％,特异度为93％。结论与常规方法相比,hs-CRP在小儿浆膜腔积液的鉴别诊断方面也具有较好的灵敏度、特异性,可作为临床小儿浆膜腔积液性质鉴别的指标。     

Sysmex XN-2000血细胞分析仪hsA模式在脑脊液细胞计数及分类中的应用评价

目的 研究Sysmex XN-2000血细胞分析仪高灵敏分析(XN hsA)模式对脑脊液(cerebrospinal fluid, CSF)细胞计数及分类的应用。方法 收集本院患者CSF标本268例。在XN hsA模式下,对红/白细胞计数的精密度、携带污染率、线性范围等进行性能验证,比较XN hsA模式与手工计数法的红/白细胞计数及分类结果两种方法间的差异,建立并验证XN hsA模式CSF细胞检测的复检规则。结果 红细胞计数精密度低、中、高值变异系数分别为9.59%、4.44%、1.60%;白细胞计数精密度低、中、高值变异系数分别为4.81%、2.17%、1.16%;红、白细胞计数携带污染率分别为0.029%、0.002%;检测范围分别为0.5～29 760×10⁶/L、0.1～8 352×10⁶/L。当15×10⁶/L6/L时,两种方法红细胞计数差异无统计学意义(P>0.05);当WBC>8×10⁶/L时,两种方法白细胞计数差异无...     

Evaluation of the correlation of automated and manual results of complete blood count in oncologic patients

The aim of this study is to evaluate the performance of Sysmex XS-800i analyzer and compare its correlation with standard manual method in oncologic patients. The complete blood count (CBC) with differential leukocyte count is one of the most frequently requested hematologic tests. In oncologic patients, it is more important because primary diagnosis and treatment follow-up are depending on CBC results. Automated hematology analyzers are designed to perform CBC with acceptable precision and accuracy, but manual slide examination still is needed to complement the analyzer results. One thousand CBC samples of oncologic patients analyzed by Sysmex XS-800i analyzer and the corresponding slides were examined independently by a skilled hematology technician and a pathologist. Their results analyzed for finding the degree of correlation between them. Our instrument showed excellent performance with a correlation coefficient of >0.91 for platelet and leukocyte differential count (except for basophil with r = 0.592). Sensitivity and specificity of identifying abnormal cells was 82.7 and 92 %, respectively. The Sysmex XS-800i hematology analyzer results are as reliable as the standard manual method; however, manual microscopic blood examination should be used to complete the uncompleted results, to validate analyzer flags, and for counting the cells with a flag.     

有核红细胞采用XN1000血液分析仪计数与人工计数结果比较

目的 Sysmex XN1000b型全自动血细胞分析仪检测的有核红细胞结果的有效性和准确性,通过金标法人工涂片检测进行验证。方法 2017年1月~2017年3月本院经过Sysmex XN1000b型全自动血细胞分析仪检测有核红细胞结果阳性标本213例进行仪器结果与人工涂片结果进行准确性验证,对随机抽取100例有核红细胞结果为零的标本验证阴性符合率验证。结果 采用Sysmex XN1000b型全自动血细胞分析仪计数有核红细胞,真阳性率100.00%,假阳性率2.09%,假阴性率0（＜5%）,真阴性率96.70%,阳性结果符合率97.56%。结论 Sysmex XN1000b型全自动血细胞分析仪检测的有核红细胞计数准确性高,阴阳性符合率好,结果可靠,有核红细胞计数可以作为临床报告项目。     

An evaluation of the Sysmex NE-8000 hematology analyzer

The Sysmex NE-8000 is a new, fully automated hematology analyzer capable of providing a five-part white blood cell differential count and identifying abnormal specimens. This instrument was evaluated on 5,000 consecutive blood specimens and compared to the Coulter S Plus-IV analyzer and manual differential cell counts to determine the efficacy of its five-cell differential and screening capabilities. There was a high correlation between the commercial counters for the standard parameters, white blood cell count, red blood cell count, hemoglobin level, hematocrit, mean corpuscular volume, mean corpuscular hemoglobin concentration, and platelet count (r greater than 0.95), except for the mean corpuscular hemoglobin concentration (r = 0.51), for which the NE-8000 was considered the more accurate measurement. Precision and linearity studies were excellent. The white blood cell count, red blood cell count, hemoglobin level, and platelet count were reproducible on specimens stored at 4 degrees C or room temperature for 72 hours and the differential counts were reproducible for 12 hours. The correlations between automated and manual counts for neutrophils, eosinophils, basophils, and lymphocytes were excellent: r = 0.912, 0.945, 0.332, and 0.964, respectively. The monocyte correlation improved with software modification from 0.306 to 0.801. The NE-8000 gave accurate and reproducible differential counts for neutrophils, lymphocytes, and monocytes on specimens with white blood cell counts as low as 0.6 x 10(9)/1. The ability of the instrument to 'flag' abnormal specimens was excellent. The false-positive rate on normal samples was 1.8%, and the false-negative rate on known abnormal samples was 0.3%, due only to nonrecognition of a mild left shift. The identification of specific abnormalities was less precise. The NE-8000 is a powerful hematology analyzer that can perform a five-part white blood cell differential count accurately for a wide range of WBCs and reliably indicate abnormal specimens. It is an excellent screening tool for distinguishing between normal and abnormal specimens and identifying those that require microscopy. Its reliability significantly reduces the need for manual film examination.     

XS-1000i血液分析仪计数嗜碱性粒细胞影响原因分析

目的分析Sysmex XS-1000i血液分析仪计数嗜碱性粒细胞部分标本出现假性增高的影响原因。方法对50例外周血中性粒细胞形态正常的标本使用Sysmex XE-5000、XS-1000i血液分析仪进行嗜碱性粒细胞计数,作相关统计学分析。4例使用Sysmex XS-1000i血液分析仪计数嗜碱性粒细胞出现明显增高标本与手工计数法进行比较,做相关形态学分析。结果 50例外周血中性粒细胞形态正常的标本使用Sysmex XE-5000、XS-1000i血液分析仪进行嗜碱性粒细胞计数,两者相关系数r=0.715,啫碱性罯赴剖钜炀咄臣蒲б庖澹≒〈0.01）。4例使用Sysmex XS-1000i血液分析仪计数嗜碱性粒细胞出现明显增高标本均出现中性粒细胞中毒颗粒、退行性变等形态学改变。结论两仪器间计数嗜碱性粒细胞结果差异有统计学意义,外周血中性粒细胞中毒颗粒等形态改变会造成XS-1000i血液分析仪计数嗜碱性粒细胞假性增高,对嗜碱性粒细胞异常增高的结果需进行手工计数分类。     

Evaluation of an automated hematology system (Technicon H-1)

We evaluated the Technicon H-1 hematology system, a new third-generation blood cell analyzer that performs a complete blood cell count, including red blood cell (RBC) morphometric studies and a full white blood cell differential count. Linearity and precision were acceptable over the entire clinical range. We compared the H-1 with the Technicon H-6000 and the Coulter S Plus IV. Close correlation was observed for all equivalent parameters except the mean corpuscular hemoglobin concentration (MCHC), the mean platelet volume, and the basophil count. Poor correlation for the MCHC reflects the novel methodology of the H-1 for measuring RBC size and, independently, RBC hemoglobin content. The H-1 MCHC correlates with hypochromia as assessed morphologically, while the MCHC of the H-6000 or S Plus IV does not. Further technological innovations include measurement of the mean neutrophil peroxidase content and nuclear lobularity.     

荧光定量PCR在筛查甲型流感病毒中的诊断价值

目的:探讨荧光定量PCR技术在筛查甲型流感病毒中的诊断价值。方法:用荧光定量RT-PCR方法检测150例疑似流感患者咽拭子中的甲型流感病毒RNA,用XT-1800全自动血液分析仪检测150例疑似流感患者EDTA抗凝全血中的白细胞计数,用胶体金法检测54例甲型流感病毒RNA阳性患者的甲型流感病毒核心蛋白。结果:在150例患者中,甲型流感病毒RNA检测阳性者有54例,阳性率为36%。54例甲型流感病毒RNA检测阳性者采用胶体金法检测甲型流感病毒核心蛋白,结果均为阴性。甲型流感病毒RNA检测阳性者白细胞计数为(6.81±2.12)×109/L,阴性者为(6.64±3.13)×109/L,白细胞计数在甲型流感患者和非甲型流感患者中无统计学差异(P0.05)。结论:荧光定量RT-PCR在检测甲型流感病毒RNA中有较好的阳性检出率,其敏感性和特异性明显优于胶体金方法和白细胞计数,能快速有效筛选出甲型流感患者,防止疫情爆发流行。     

The lack of clinical utility of white blood cell differential counts and blood morphology in elderly individuals with normal hematology profiles

An investigation of 462 patients aged older than 65 years with normal hematology profiles revealed 105 individuals with unsuspected abnormalities in the white blood cell differential count, peripheral blood film, or platelet count. In only 8 patients would this information have resulted in additional patient testing or other changes in management. The availability of instrumentation providing an automated platelet count would have decreased the number of patients experiencing a change in management to 5. In no instance did physicians believe that an individual patient's clinical outcome would have been altered by a knowledge of the unexpected abnormalities or the anticipated management changes. In an ambulatory adult population with normal hematology profiles, there is limited clinical value in pursuing further studies, such as a white blood cell differential count or an examination of the red blood cell morphology.     

The new hematology analyzer Sysmex XE-2100: performance evaluation of a novel white blood cell differential technology

CONTEXT: The new hematology analyzer Sysmex XE-2100 (TOA Medical Electronics, Kobe, Japan) has a novel, combined, white blood cell differential technology and a special reagent system to enumerate nucleated red blood cells. DESIGN: Performance evaluation of both technologies of the Sysmex XE-2100 according to the H20-A protocol of the National Committee for Clinical and Laboratory Standards and comparison of the results with those for the hematology analyzer Sysmex NE-8000 (TOA Medical Electronics). SPECIMENS: Five hundred forty-four blood samples randomly chosen from various inpatient and outpatient departments of the Vienna University hospital. RESULTS: Five-part white blood cell differential counts on the XE-2100 revealed excellent correlation with the manual reference method for neutrophils, lymphocytes, and eosinophils (r =.925,.922, and.877, respectively) and good correlation for monocytes and basophils (r =.756 and.763, respectively). The efficiency rates of flagging for the presence of >/=1% abnormal white blood cells were 83% (XE-2100) and 66% (NE-8000). The correlation of automated and microscopic nucleated red blood cell counts was excellent (r =.97). CONCLUSIONS: From the present evaluation and our former experience with other types of Sysmex analyzers, we conclude that the new white blood cell differential technology of the XE-2100 represents a further development toward more efficient flagging of abnormal white blood cells.     

Evaluation of the Sysmex CC-800. An automated eight-parameter hematology instrument

An evaluation of the Sysmex CC-800 hematology analyzer (TOA Medical Electronics, Kobe, Japan, and distributed by American Scientific Products, Chicago, IL) was performed at University Hospital at Boston University Medical Center to assess the analytic performance and ease of use of the instrument. The Sysmex CC-800 is the first self-contained, fully automated, eight-parameter hematology analyzer. It can handle as many as 100 whole blood specimens without constant operator intervention. Stat and predilute modes are also available. The optional PDA-410 (particle distribution analyzer) was not evaluated in this study. Precision, linearity, carryover, and reproducibility of values over time in the automode were well within the manufacturer's specifications. The correlation study was performed with the existing Coulter S-Plus (Coulter Electronics, Hialeah, FL). The Sysmex CC-800 allowed us to expand the linear range without dilution for white blood cells to 160 X 10(3)/microL, hemoglobin to 26 g/dL, and platelets to 2,000 X 10(3)/microL. The samples on the automode exposed to normal laboratory atmosphere for up to 120 minutes showed no significant difference from baseline. The two instruments correlated well (r greater than 0.99). The authors concluded that the Sysmex CC-800 would be a reliable and time saving instrument in their laboratory.     

Use of an automated hematology analyzer and flow cytometry to assess bone marrow cellularity and differential cell count

The automated analysis of bone marrow aspirates was performed to estimate the bone marrow cellularity and differential cell count. Total nucleated cell count (TNC) was measured using an automated hematology analyzer. TNC of the bone marrow correlated well with the bone marrow cellularity estimated by microscopic examination (r = 0.590, p <0.001). The bone marrow cellularity was readily confirmed as <30%, from 30 to 70%, or >70% according to TNC. Differential count of bone marrow cells was done with the combination of CD45 monoclonal antibody and propidium iodide using flow cytometry. Excellent correlations were obtained for the population distributions of normoblasts, eosinophils, lymphocytes, and blasts between the results of flow cytometry and manual differential counts. The percentage of mature granulocytes in flow cytometry showed good correlation with the manual percentage of metamyelocytes + neutrophils. The percentage of immature granulocytes by flow cytometry showed good correlation with the manual percentage of blasts + promyelocytes + myelocytes. These results demonstrate that analysis of bone marrow aspirates using an automated hematology analyzer is valuable in the determination of bone marrow cellularity. Moreover, as flow cytometry provides objective findings for percentages of major cell populations, it can serve as a method to automate bone marrow differentials.