2008-2010年江门市腹泻病原菌现状调查

目的了解江门市细菌性腹泻病原菌的种类和构成特点,为预防和临床治疗提供科学的参考依据。方法 2008年12月至2010年6月,采集监测点腹泻患者的粪便（或肛拭）,分离霍乱弧菌、志贺菌、沙门菌、出血大肠O157：H7、空肠弯曲菌、结肠耶尔森菌、副溶血性弧菌、变形杆菌等10种病原菌,采用传统分离培养法及BD PHOENZX SYSTEM全自动鉴定系统鉴定。结果 1260份标本共分离各类病原菌189株,检出率为15.00%,其中检出金黄色葡萄球菌61株,沙门菌52株,蜡样芽胞杆菌22株,变形杆菌24株,副溶血性弧菌24株,空肠弯曲菌2株,结肠耶尔森菌1株,志贺菌3株,霍乱、出血大肠O157：H7未检出。结论金黄色葡萄球菌、沙门菌是江门市细菌性腹泻的主要病原菌。     

2007—2008年北京大学深圳医院急性感染性腹泻细菌病原学监测结果分析

目的了解2007年7月至2008年7月间北京大学深圳医院急性感染性腹泻的流行病学特征。以便及时采取措施以防传染病暴发流行。方法选择每日排便≥3次的患者作为研究对象,在患者未使用抗菌药物之前进行大便细菌病原学检验并进行统计分析。结果共检测1017份标本,检出病原菌133株,阳性率为13．07％。133株病原菌中,致病性大肠埃希菌、副溶血弧菌、产毒大肠埃希菌、侵袭性大肠埃希菌、沙门菌分别分离出43株（32．34％）、37株（27．82％）、19株（14．28％）、18株（13．54％）和13株（9．77％）。夏秋季检出肠道致病菌90株（67．66％）,高于春冬季节的43株（32．34％）。结论不仅要针对各病原菌检出率的差异在不同季节开展相应的防控措施,还要结合地域的特点对特定的病原菌进行重点监测。     

儿童感染性腹泻病原微生物、耐药性分析

目的:研究儿童感染性腹泻病原微生物、耐药情况.方法:选取濮阳市某医院2018年12月至2020年12月收治的感染性腹泻儿童100例,分析其感染病原和耐药性情况.结果:100份粪便共培养出97株病原微生物.检出病毒中,诺如病毒和A组轮状病毒比例较高;肠道细菌中,致病性大肠埃希菌的比例较高.经检测,致病性大肠埃希菌对新生霉素和利福平的耐药性为100％,对四环素和氨苄西林的耐药性为70.11％,对多粘菌素B和阿米卡星的敏感度为100％.结论:感染性腹泻儿童的主要感染病原为诺如病毒、A组轮状病毒、致病性大肠埃希菌和沙门菌,且细菌具有多重耐药性.     

医院内泌尿道感染患者的病原菌耐药性分析

目的 了解湖北省襄阳市第一人民医院住院患者泌尿系感染的主要病原菌分布与抗生素对常用菌的 耐药率。方法 回顾性收集湖北省襄阳市第一人民医院2017年1月~12月住院患者1580份尿培养样本,采用全自动微生物鉴定仪480份阳性标本进行鉴定及药敏试验。应用WHONET5.6对数据进行分析。结果 480株病原菌中,革兰阴性杆菌353株（73.54%）,以大肠埃希菌、肺炎克雷伯菌和铜绿假单胞菌为主;革兰阳性球菌85株占（17.71%）,以肠球菌和凝固酶阴性葡萄球菌为主;真菌40株（8.33%）。检出的病原菌对各种抗生素的耐药率不同,所有分离的大肠埃希菌与肺炎克雷伯菌对三代头孢菌素和喹诺酮类耐药率均大于50%,并且外科系统分离的大肠埃希菌株耐药率高于内科系统;铜绿假单胞菌对β内酰胺酶抑制剂耐药率大于30%,高于其他病原菌对此类抗生素耐药率。未发现分离的革兰阳性菌对万古霉素和利奈唑胺耐药,凝固酶阴性葡萄球菌对苯唑西林耐药率达82.60%。结论 本院泌尿系统感染中,以大肠埃希菌感染为主,β内酰胺酶抑制剂可用于大肠埃希菌和肺炎克雷伯菌常规治疗。     

社区获得性泌尿系统感染病原菌分布及耐药性分析

目的 分析社区获得性泌尿系统感染病原菌分布及耐药性,为临床合理使用抗菌药物提供依据, 减少耐药菌株的产生。方法 收集2015年1月~2019年1月绵阳科学城医院门诊疑似泌尿系统感染患者中段尿标本分离培养的病原菌及其体外药敏试验结果,并对结果进行统计分析。结果 共分离187株泌尿系统感染细菌,以大肠埃希菌、肺炎克雷伯菌、肠球菌、葡萄球菌为主,其中革兰阴性杆菌148株,占79.14%,检出产ESBLs大肠埃希菌82株,产ESBLs肺炎克雷伯菌13株;革兰阳性球菌37株,占19.78%。药敏结果分析显示大肠埃希菌、肺炎克雷伯菌对碳青霉烯类和头霉素类抗菌药物高度敏感,其次为哌拉西林+他唑巴坦、呋喃妥因（平均耐药率＜10.00%）;粪肠球菌、葡萄球菌对万古霉素、利奈唑胺、呋喃妥因完全敏感。结论 社区泌尿系统感染病原菌以革兰阴性杆菌为主,大肠埃希菌产酶率高,耐药现象较严重;临床应根据当地病原菌构成特点及药敏试验结果合理使用抗菌药物,避免经验性用药,控制耐药性菌株的播散,提高治疗效果。     

313例尿培养病原菌分布及耐药性分析

目的分析313例清洁中段尿培养菌株构成及耐药性分析,为医师合理规范使用抗生素提供依据。方法对医院2012年6月~2013年5月住院泌尿系感染患者尿培养分离出的313株病原菌用法国梅里埃公司VITEK 2 COMPACT全自动微生物鉴定药敏检测系统仪进行鉴定和药敏试验。结果共培养1804份中段尿标本,分离出313例病原微生物,阳性率17.35%。其中革兰阴性杆菌、革兰阳性球菌、真菌、革兰阳性杆菌分别占65.50%、19.81%、7.35%、7.35%。大肠埃希菌仍是泌尿系感染的主要致病菌,其次为肺炎克雷伯杆菌、阴沟肠杆菌、铜绿假单胞菌、奇异变形菌;其中产超广谱β-内酰胺酶(ESBLs)大肠埃希菌占大肠埃希菌41.94%;而在革兰阳性菌中以肠球菌属为主。检出耐亚胺培南的肺炎克雷伯菌1株,耐碳青霉烯类阴沟肠杆菌2株,耐万古霉素屎肠球菌1例。结论泌尿系感染常见病原菌仍为大肠埃希菌,临床应根据尿培养及药敏试验结果有针对性筛选敏感药物进行治疗。     

我院急性阑尾炎手术患者细菌学分析及药敏试验

目的 了解引起急性阑尾炎患者感染的痛原菌分布及对药物的耐药性,有助于指导临床合理选择抗菌药物.方法 回顾分析2013年所有阑尾炎手术患者细菌培养及药敏试验结果.结果 382例标本中有157例检出细菌(其中16例为两种细菌混合感染),阳性率为41.1％,分离到细菌173株,其中革兰阴性菌167株(96 5％),革兰阳性菌6株(3.5％);从分离到的细菌构成前3位是大肠埃希菌、肺炎克雷伯菌、铜绿假单胞菌,分别占检出病原菌的67.1％、9 8％、6 9％;大肠埃希菌体外药敏试验结果:对头孢菌素类敏感率＞60％;喹诺酮类敏感率＜50％;头孢替坦、哌拉西林他唑巴坦和阿米卡星敏感率达到99％以上;无对碳青霉烯类耐药株.结论 我院急性阑尾炎患者感染菌中以阴性肠杆菌为主,其中以大肠埃希菌占首位,临床抗菌药物选择可以首选头霉素类和阿米卡星,头孢菌素类亦可以经验选择.     

南京市江宁医院2015年~2017年血培养阳性病原菌分布及耐药性分析

目的 研究血培养阳性检出的病原菌分布及耐药情况,指导临床合理用药。方法 采用法国梅里埃公司Bact/Alert 3D全自动血培养仪对我院2015年1月~2017年12月送检的血培养标本进行培养,阳性标本用VITEK 2 Compact全自动细菌鉴定及药敏分析系统进行细菌鉴定和药敏试验,观察病原菌分布及耐药情况。结果 共分离出病原菌459株,检出率为9.57%,其中革兰阴性杆菌246株（53.59%）,革兰阳性球菌202株（44.01%）,真菌11株（2.40%）;前3位病原菌依次为大肠埃希菌、肺炎克雷伯菌、金黄色葡萄球菌。大肠埃希菌对美罗培南、阿米卡星、厄他培南、亚胺培南均较敏感,耐药率低于3%。肺炎克雷伯菌对美罗培南、阿米卡星、厄他培南、亚胺培南、头孢替坦较敏感,耐药率低于6%。未检出对万古霉素、利奈唑胺、喹努普汀/达福普汀、替加环素耐药的金黄色葡萄球菌。结论 本院血培养的病原菌种类多,以革兰阴性杆菌为主,且耐药情况复杂。应加强对病原菌的鉴定和药敏监测,指导临床合理用药,防止广谱抗生素的滥用,减少耐药菌株的产生。     

重症监护室6400例血培养病原菌分布及药敏分析

目的对重症监护室6400例血培养的病原菌进行分离,并对菌株的分布及药敏进行分析。方法回顾调查2011年1月至2013年6月高州市人民医院ICU病房6400例患者的血样标本,并对其病原菌种类进行分离。采用BDBACTECTM9120全自动血培养仪对血液进行培养,BD—Phoenix-100全自动细菌鉴定仪对细菌的种类进行鉴定并对细菌的药敏性进行检测。结果6400例血样中共检出细菌971株,阳性率为15．17％。其中革兰阴性菌检出515株,占53．04％;革兰阳性菌425株,占43．77％;真菌31株,占3．19％。检出病原菌主要为大肠埃希菌、铜绿假单胞菌、金黄色葡萄球菌、表皮葡萄球菌、肺炎克雷伯菌以及肠球菌等。其中,葡萄球菌对于甲氧西林和青霉素的耐药性最大,大肠埃希菌、肺炎克雷伯菌以及铜绿假单胞菌对亚胺培南都有很好的受药性。结论阴性葡萄球菌、鲍曼不动杆菌等在重症监护室中属于多发常见的病原菌,该种菌的耐药性以及多重耐药性都非常普遍,临床上应根据药敏检测结果进行用药。     

大肠埃希菌的耐药特性及其产超广谱β-内酰胺酶的分析

观察大肠埃希菌的耐药特性及产超广谱β-内酰胺酶(ESBLs)的情况，为临床正确选择抗生素提供药敏依据。用微量稀释法测定最低抑菌浓度(MIC)，用ESBLs表型确证试验检测出产ESBLs的大肠埃希菌。共检出314株大肠埃希菌。对其中298株大肠埃希菌进行了ESBLs检验，检出产ESBLs菌81株，检出率为27．2％；81株产ESBLs菌对多种药物耐药率较高，除亚胺培南、头孢哌酮，舒巴坦、庆大霉素和阿米卡星外，大肠埃希菌产ESBLs株对其他9种抗生素的耐药性明显高于非产ESBLs株。大肠埃希菌产ESBLs率高，应引起临床高度重视，对检测出的大肠埃希菌均应进行ESBLs检测，产ESBLs细菌有较高的交叉耐药性和多重耐药性，对亚胺培南和头孢哌酮，舒巴坦的敏感性好。     

Use of an alkaline phosphatase-labeled synthetic oligonucleotide probe for detection of Campylobacter jejuni and Campylobacter coli.

A commercially available synthetic nucleic acid probe (SNAP) conjugated to alkaline phosphatase was compared with standard culture techniques for detecting Campylobacter species. The SNAP was able to detect either 5 ng of C. jejuni DNA or 10(5) CFU of bacteria. The SNAP could also detect DNA extracted from 10(5) CFU in mock-infected stool samples. The SNAP detected C. jejuni and C. coli but showed no reactivity with C. laridis, C. fetus subsp. fetus, C. fetus subsp. venerealis, C. fennelliae, "C. upsaliensis," C. cinaedii, C. fecalis, C. hyointestinalis, C. mucosalis, or Helicobacter (Campylobacter) pylori. The SNAP also showed no cross-reactivity with other enteric pathogens. When applied to pure cultures, the SNAP detected 55 clinical isolates of C. jejuni and 11 clinical isolates of C. coli, with an accuracy of 100%. When applied directly to clinical specimens, the SNAP detected Campylobacter spp. in 19 of 23 culture-positive stool specimens (sensitivity, 82.6%; specificity, 100%). Pure cultures of the Campylobacter strains isolated from the four probe-negative, culture-positive stool specimens gave positive reactions with the SNAP. While the SNAP had excellent sensitivity and specificity for isolated bacterial colony isolates, the main limitation to the Campylobacter probe detection kit may be the sensitivity limit on direct detection of Campylobacter organisms in stools.     

Parasitic, bacterial, and viral enteric pathogens associated with diarrhea in the Central African Republic.

A total of 1,197 diarrheic children less than 15 years old were investigated for parasitic, bacterial, and viral enteropathogens from March 1981 through February 1982 in the Central African Republic. One or more pathogens were identified from 49.4% of the patients. Rotavirus was the most frequently identified pathogen among children less than 18 months old. Enteropathogenic Escherichia coli was the second most frequently isolated pathogen (12.1%) in children less than 2 years of age. Campylobacter jejuni was also isolated frequently from diarrheic children less than 5 years of age (10.9%). Entamoeba histolytica was identified in very young children and was found to be the most frequent enteropathogen associated with diarrhea in children over the age of 2 years. Enterotoxigenic Escherichia coli was rarely isolated (ca. 2%). There was a peak in the incidence of rotavirus during the dry season and in the incidence of Campylobacter jejuni during the rainy season.     

Development and evaluation of immunochromatographic assay for simple and rapid detection of Campylobacter jejuni and Campylobacter coli in human stool specimens

An immunochromatographic assay (Campy-ICA) using a newly generated single monoclonal antibody against a 15-kDa cell surface protein of Campylobacter jejuni was developed. When cell suspensions of 86 C. jejuni strains and 27 Campylobacter coli strains were treated with a commercially available bacterial protein extraction reagent and the resulting extracts were tested with the Campy-ICA, they all yielded positive results. The minimum detectable limits for the C. jejuni strains ranged from 1.8 x 10(4) to 8.2 x 10(5) CFU/ml of cell suspension, and those for the C. coli strains ranged from 1.4 x 10(5) to 4.6 x 10(6) CFU/ml of cell suspension. All 26 non-Campylobacter species tested yielded negative results with the Campy-ICA. To evaluate the ability of the Campy-ICA to detect C. jejuni and C. coli in human stool specimens, suspensions of 222 stool specimens from patients with acute gastroenteritis were treated with the bacterial protein extraction reagent, and the resulting extracts were tested with the Campy-ICA. The Campy-ICA results showed a sensitivity of 84.8% (28 of 33 specimens) and a specificity of 100% (189 of 189 specimens) compared to the results of isolation of C. jejuni and C. coli from the stool specimens by a bacterial culture test. The Campy-ICA was simple to perform and was able to detect Campylobacter antigen in a fecal extract within 15 min. These results suggest that Campy-ICA testing of fecal extracts may be useful as a simple and rapid adjunct to stool culture for detecting C. jejuni and C. coli in human stool specimens.     

Evaluation of the ProSpecT Microplate Assay for detection of Campylobacter: a routine laboratory perspective

Objectives  To evaluate the use of the new enzyme-linked immunosorbent assay, the ProSpecT Campylobacter Microplate Assay (Alexon-Trend, Minneapolis, MN, USA), which allows 2-h detection of both Campylobacter jejuni and Campylobacter coli antigen directly in stool specimens.
Methods  Over 4 months, all stool samples preserved in Cary–Blair medium, or fresh specimens, from non-hospitalized children and HIV-infected patients (adults and children), submitted to our laboratory were evaluated with the ProSpecT Campylobacter Microplate Assay. Results were compared with those obtained by routine culture methods using both a specific medium and a filtration method for the recovery of Campylobacter spp.
Results  Of the 1205 stool specimens cultured, 101 were found to be positive for either C. jejuni or C. coli , giving an overall recovery rate of 8.38%. Ninety samples were positive by both culture and ProSpecT Campylobacter Microplate Assay, and 11 were positive by culture only, giving a sensitivity of 89.1%. In addition, of 1104 samples negative by culture, 25 were initially positive by ProSpecT Campylobacter Microplate Assay. We found no cross-reaction with other bacterial enteropathogens isolated from stool specimens. These results thus confirm a high specificity (97.7%) for both C. jejuni and C. coli. The positive and negative predictive values found were 78.3% and 99%, respectively. There was no statistically significant difference in sensitivity and specificity if the stool was fresh or preserved with Cary–Blair medium.
Conclusion  These data suggest that the ProSpecT Campylobacter Microplate Assay is a rapid and easy-to-use test for the detection of both C. jejuni and C. coli in stool specimens. It could be used for patients for whom early antibiotic therapy is needed or for epidemiologic studies.     

Specific detection of Campylobacter jejuni and Campylobacter coli by using polymerase chain reaction.

Development of a routine detection assay for Campylobacter jejuni and Campylobacter coli in clinical specimens was undertaken by using the polymerase chain reaction (PCR). An oligonucleotide primer pair from a conserved 5' region of the flaA gene of C. coli VC167 was used to amplify a 450-bp region by PCR. The primer pair specifically detected 4 strains of C. coli and 47 strains of C. jejuni; but it did not detect strains of Campylobacter fetus, Campylobacter lari, Campylobacter upsaliensis, Campylobacter cryaerophila, Campylobacter butzleri, Campylobacter hyointestinalis, Wolinella recta, Helicobacter pylori, Escherichia coli, Shigella spp., Salmonella spp., Vibrio cholerae, Citrobacter freundii, or Aeromonas spp. By using a nonradioactively labeled probe internal to the PCR product, the assay could detect as little as 0.0062 pg of purified C. coli DNA, or the equivalent of four bacteria. In stools seeded with C. coli cells, the probe could detect between 30 and 60 bacteria per PCR assay. The assay was also successfully used to detect C. coli in rectal swab specimens from experimentally infected rabbits and C. jejuni in human stool samples.     

Prevalence of enteroaggregative Escherichia coli among children with and without diarrhea in Switzerland

In a prospective study between July 1999 and September 2000, stool specimens of children below the age of 16 years with (n = 187) and without (n = 137) diarrhea were tested for the presence of enterovirulent bacteria by standard culture methods and by PCR. Targets for the PCR were the plasmid pCVD432 for enteroaggregative Escherichia coli (EAEC), the verotoxin 1 and verotoxin 2 genes for enterohemorrhagic E. coli, ipaH for enteroinvasive E. coli (EIEC) and Shigella spp., genes coding for heat-stable and heat-labile toxins for enterotoxigenic E. coli (ETEC), and the eaeA gene for enteropathogenic E. coli. The following bacteria could be associated with diarrhea: Salmonella enterica (P = 0.001), Campylobacter spp. (P = 0.036), ETEC (P = 0.012), and EAEC (P = 0.006). The detection of EAEC, ETEC, and S. enterica was strongly associated with a history of recent travel outside of Switzerland. EAEC isolates were found in the specimens of 19 (10.2%) of 187 children with diarrhea and in those of 3 (2.2%) of 137 children without diarrhea (P = 0.006) and were the most frequently detected bacteria associated with diarrhea. Among the children below the age of 5 years, the specimens of 18 (11.9%) of 151 with diarrhea were positive for EAEC, while this agent was found in the specimens of 2 (2.2%) of 91 controls (P = 0.007). Enteropathogenic E. coli isolates were found in the specimens of 30 (16.4%) of the patients and in those of 15 (10.9%) of the controls, with similar frequencies in all age groups (P > 0.05). We conclude that EAEC bacteria are involved in a significant proportion of diarrhea cases among children. Children younger than 5 years of age are more often affected by EAEC than older children.     

Evaluation of a latex agglutination method for the rapid identification of Campylobacter jejuni/coli

A latex agglutination assay was developed to identify Campylobacter jejuni and Campylobacter coli. We evaluated the specificity, reproducibility and utility of the assay for clinical use and the following results were obtained. 1) To prepare standardized antigen, bacterial cells must be suspended to a density of 1 to 5 McFarland unit, and heated at 121 degrees C for 10 to 30 min. 2) Bacterial cells may be suspended either in the solution provided with the kit, or in physiological saline, without affecting the results. 3) Of C. jejuni, 94 strains, 6 of C. coli, and 3 of "Campylobacter upsaliensis", all tested positive without exception. All other Campylobacter species, encompassing 13 species and 80 strains, were negative. An additional 9 species and 30 strains, of non-Campylobacter gram negative bacteria, isolated on the Campylobacter selection agar medium, also were uniformly negative. Based on these results, we conclude that bacteria testing positive with the kit can be identified as C. jejuni/coli. Interestingly, "C. upsaliensis", although isolated very rarely from the clinical specimens, also tested positive.     

Duplex real-time SYBR green PCR assays for detection of 17 species of food- or waterborne pathogens in stools

A duplex real-time SYBR Green LightCycler PCR (LC-PCR) assay with DNA extraction using the QIAamp DNA Stool Mini kit was evaluated with regard to detection of 8 of 17 species of food- or waterborne pathogens in five stool specimens in 2 h or less. The protocol used the same LC-PCR with 20 pairs of specific primers. The products formed were identified based on a melting point temperature (T(m)) curve analysis. The 17 species of food- or waterborne pathogens examined were enteroinvasive Escherichia coli, enteropathogenic E. coli, enterohemorrhagic E. coli, enterotoxigenic E. coli, enteroaggregative E. coli, Salmonella spp., Shigella spp., Yersinia enterocolitica, Yersinia pseudotuberculosis, Campylobacter jejuni, Vibrio cholerae, Vibrio parahaemolyticus, Vibrio vulnificus, Aeromonas spp., Staphylococcus aureus, Clostridium perfringens, and Bacillus cereus. No interference with the LC-PCR assay was observed when stool specimens were artificially inoculated with each bacterial species. The detection levels were approximately 10(5) food- or waterborne pathogenic bacteria per g of stool. The protocol for processing stool specimens for less than 10(4) food- or waterborne pathogenic bacteria per g of stool requires an overnight enrichment step to achieve adequate sensitivity. However, the rapid amplification and reliable detection of specific genes of greater than 10(5) food- or waterborne pathogenic bacteria per g in samples should facilitate the diagnosis and management of food- or waterborne outbreaks.     

Importance of salmonellae and Campylobacter jejuni in the etiology of diarrheal disease among children less than 5 years of age in a community in Bangkok, Thailand.

The etiology of diarrhea in children less than 5 years of age in a low-income housing project in Bangkok, Thailand, was determined over 1 year. Nontyphoidal salmonellae (13%), Campylobacter jejuni (12%), rotavirus (12%), enterotoxigenic Escherichia coli (7%), shigellae (6%), E. coli that hybridized with the enteropathogenic E. coli adherence factor probe (3%), and enteroinvasive E. coli (1%) were identified in 345 episodes of diarrhea in children less than 5 years of age. Salmonellae were identified in 17% and C. jejuni was identified in 15% of 54 children less than 6 months of age with diarrhea. Shigellae, enteroinvasive E. coli, enteropathogenic E. coli adherence factor, and enterotoxigenic E. coli were not isolated from children less than 6 months of age. Since salmonellae and C. jejuni were the most common bacterial pathogens identified in children less than 6 months of age, efforts to prevent transmission of salmonellae and campylobacter to young children should be a public health priority in Bangkok.     

腹泻相关致病菌基因芯片的制备及应用

目的确定引起人类感染性腹泻的11种病原微生物,并制备芯片用于检测门诊腹泻患者粪便标本中的致病菌。方法根据本院2009年1月至2012年12月期间腹泻门诊的粪便病原菌检测数据,采用生物信息学的方法,收集11种病原菌的所有基因序列,设计引物及探针,优化并制备芯片,PCR扩增杂交并对杂交结果进行分析。用该芯片对本院保存的163个肠道致病菌临床分离株进行鉴定来评价芯片的特异性,用芯片来检测掺有不同浓度沙门氏菌的粪便标本评价芯片的灵敏度。同时收集2010年6月至2013年3月在本院就诊的1052份腹泻患者粪便标本,平行进行PCR扩增、细菌培养、基因芯片检测,比较不同检测方法的阳性率。结果成功制备了腹泻相关11种致病菌检测芯片。应用制备的芯片检测了163个临床分离株,准确率达100％。与PCR方法比较,基因芯片检测沙门氏菌的灵敏度达102CFU／ml,比PCR法检测灵敏度高10倍。用该芯片对临床1052份腹泻患者腹泻标本进行检测,与传统的培养法及PCR法比较,有较高的阳性检出率,达36％,比常规细菌培养阳性率高13％（X2=2．28,P〈0．05）,比PCR检出率高4％（X2=5．16,P〉0．05）。结论成功制备11种腹泻相关致病菌基因芯片,能同时对11种腹泻致病菌进行检测,有较好的特异性和灵敏度,有更高的阳性率,可以用于临床检测。