骨髓增生异常综合征患者红细胞免疫黏附功能的研究

目的 探讨骨髓增生异常综合征(MDS)患者红细胞免疫黏附功能的变化及其与疗效及预后的关系.方法 应用红细胞酵母菌花环试验测定38例健康献血者(对照组)和35例初次确诊为MDS患者(患者组)缓解前后红细胞膜C3b受体花环率(RBC-C3bRR)及红细胞膜免疫复合物花环率(RBC-ICR).结果 对照组RBC-C3bRR及RBC-ICR分别为(18.65±2.58)%和(7.45±2.16)%;患者组缓解前后RBC-C3bRR分别为(9.87±2.43)%和(14.65±3.22)%,而缓解前后RBC-ICR分别为(15.48 ±3.25)%和(9.36±2.56)%.患者组缓解前后RBC-C3bRR及RBC-ICR与对照组比较差异均有统计学意义(P均＜0.01);患者组缓解前后RBC-C3bRR及RBC-ICR比较差异有统计学意义(P＜0.01);缓解后RBC-C3bRR明显升高,但仍较对照组低,缓解后RBC-ICR明显降低,但仍较对照组高.结论 MDS患者红细胞免疫黏附功能下降,测定红细胞免疫黏附功能(RBC-C3bRR、RBC-ICR)可作为判定MDS患者疗效及预后的指标之一.     

饮酒对成人红细胞免疫及其调节功能影响的研究

目的 探讨饮酒对红细胞免疫及其调节功能的影响.方法 对饮酒组(94人)和对照组(62人)健康成人分别检测其红细胞受体花环率(RBC-C3bRR)、红细胞免疫复合物花环率(RBC-ICR)、红细胞免疫增强因子(EF)及抑制因子(IF)的变化.结果 饮酒组的RBC-C3bRR、EF水平低于非饮酒组(P均＜0.01),而RBC-ICR水平则高于非饮酒对照组(P＜0.01);相关分析表明RBC-C3bRR、EF水平与饮酒量及饮酒时间负相关,而RBC-ICR、IF水平则与被动饮酒量及饮酒时间呈正相关.结论 长期大量饮酒可引起继发性免疫功能低下及降低红细胞免疫调节能力.     

新生儿缺氧缺血性脑病的红细胞免疫功能变化研究

目的 了解新生儿缺氧缺血性脑病(HIE)的红细胞免疫功能状态及病情严重程度不同的患儿免疫功能是否不同.方法 HIE组为新生儿HIE患儿68例,其中轻度26例,中度22例,重度20例.对照组30例新生儿为同期足月健康新生儿.采用细胞酵母花环法检测红细胞C3b受体花环率(RBC-C3bRR)、红细胞免疫复合物花环率(RBC-ICR).并进行统计学分析.结果 HIE组患儿的RBC-C3bRR低于对照组,RBC-ICR高于对照组,差异有统计学意义(P<0.05),重度HIE组患儿的RBC-C3bRR[(13.025±1.856)%]低于轻、中度HIE组[分别为(16.107±1.317)%、(16.156±2.935)%](P<0.05).结论 新生儿HIE的红细胞免疫功能低下,而且与病情严重程度有关.     

新生儿红细胞免疫功能测定及影响因素的研究

【目的】了解新生儿红细胞免疫功能变化,观察围产因素对新生儿红细胞免疫功能的影响。【方法】采用红细胞酵母花环法测定新生儿红细胞C3b受体花环率(the adherence rate of complement 3b-receptor onthe surface ofred blood cell,RBC-C3bRR)和红细胞免疫复合物受体花环率(the i mmune complex adherence rate of red blood cell,RBC-ICR)。【结果】①104例正常新生儿的RBC-C3bRR为:16.80±1.56;RBC-ICR为:5.72±1.63。母婴配对分析表明产妇的RBC-ICR与新生儿的RBC-ICR具有高度正相关(P<0.01);②52例高胆红素血症新生儿RBC-C3bRR为:13.59±1.55,RBC-ICR为:4.47±1.37,低于正常新生儿(P<0.01);③73例缺氧缺血性脑病新生儿RBC-C3bRR为:13.37±1.68;RBC-ICR为:4.39±1.50;低于正常新生儿(P<0.01);④新生儿RBC-C3bRR和RBC-C3bRR与胎龄、日龄、性别、羊水...     

饮酒成年男子红细胞免疫状态与超氧化物歧化酶和过氧化脂质关系的研究

[目的]探讨饮酒者体内红细胞免疫状态改变,以及其与超氧化物歧化酶和过氧化脂质的关系.[方法]对饮酒组(118人)和非饮酒对照组(53人)健康成年男子分别检测其红细胞受体花环率(RBC-C3bRR)、红细胞免疫复合物花环率(RBC-ICR)、红细胞超氧化物歧化酶(SOD)以及血浆过氧化物脂质(LPO)的变化.[结果]饮酒组的RBC-C3bRR、SOD水平均显著低于非饮酒对照组,而RBC-ICR、 IPO水平则显著高于非饮酒对照组;相关分析表明SOD水平随着饮酒量的增加而下降,两者呈负相关关系(r=-0.725, P＜0.01),而LPO则随饮酒量的增加而增高,两者呈正相关关系(r=0.916, P＜0.01);直线相关回归分析表明RBC-C3bRR与SOD星正相关(r=0.657, P＜0.05),而与LPO呈负相关(r=-0.872, P＜0.01),RBC-ICR与LPO星正相关关系(r=0.617, P＜0.05).[结论]饮酒可引起继发性免疫功能低下,其可能与体内的超氧化物歧化酶相对不足及过氧化物脂质增加有关.     

吸烟成年男子红细胞免疫状态与超氧化物歧化酶和过氧化脂质关系的研究

目的探讨吸烟者体内红细胞免疫状态改变,以及其与超氧化物歧化酶和过氧化脂质的关系.方法将158名健康成年男子分成两组吸烟组(116人)和非吸烟对照组(42人),分别检测其红细胞受体花环率(RBC-C3bRR)、红细胞免疫复合物花环率(RBC-ICR)、红细胞超氧化物歧化酶(SOD)以及血浆过氧化脂质(LPO)的变化.结果吸烟组的RBC-C3bRR、SOD水平均显著低于非吸烟对照组,而RBC-ICR、LPO水平则显著高于非吸烟对照组;相关分析表明SOD水平随着吸烟量的增加而下降,两者呈负相关关系(r=-0.584,P＜0.05),而LPO则随吸烟量的增加而增高,两者呈正相关关系(r=0.828,P＜0.01);直线相关回归分析表明RBC-C3bRR与SOD呈正相关(r=0.785,P＜0.01),而与LPO呈负相关(r=-0.913,P＜0.01).结论提示吸烟可引起继发性免疫功能低下,可能与体内的超氧化物脂歧化酶相对不足及过氧化脂质增加有关.     

黄芪注射液联合重组人促红细胞生成素对血液透析患者红细胞免疫功能的影响

目的 观察黄芪注射液联合重组人促红细胞生成素(rHuEPO)对血液透析患者红细胞免疫功能的影响.方法 51例慢性肾衰竭尿毒症行血液透析维持治疗的患者,按随机数字表法分为治疗组(26例)与对照组(25例).治疗组在透析液A液中加入黄芪注射液1 ml/kg,对照组不使用黄芪注射液.测定治疗前后IgG、IgA、IgM、红细胞C3b受体花环率(RBC-C3bRR)、红细胞免疫复合物花环率(RBC-ICR)、CD35阳性红细胞百分率、红细胞超氧化物歧化酶(SOD)水平.结果 治疗8周后,治疗组IgG、IgA、IgM水平及对照组IgG、IgA水平显著高于治疗前(P<0.01);治疗组治疗8周后IgM水平为(2.03±0.73)g/L,与对照组比较差异有统计学意义(P<0.05).两组RBC-C3bRR、CD35阳性红细胞百分率、SOD水平治疗8周后显著高于治疗前,RBC-ICR水平显著低于治疗前(P<0.05);治疗组治疗8周后,RBC-C3bRR为(10.85±2.31)%,显著高于对照组,RBC-ICR为(5.34±1.91)%,显著低于对照组(P<0.05).结论 黄芪注射液联合rHuEPO可提高血液透析患者血清免疫球蛋白水平,提高红细胞免疫功能,较单用r-HuEPO效果更好.     

肺癌患者红细胞免疫功能的研究

目的:对肺癌患者红细胞免疫功能进行研究。方法:采用受体粘附法测定48例肺癌患者的红细胞免疫功能,并与35例正常人对照分析。结果:肺癌患者红细胞C3b受体花环(RBC-C3bRR)、肿瘤红细胞花环(RBC-TRR)明显低于正常人(P<0.01),而红细胞免疫复合物花环(RBC-ICR)则明显高于正常人(P<0.01)。结论:肺癌患者红细胞免疫功能低下,检测其红细胞免疫功能有助于肺癌的诊断及治疗。     

新生儿ABO溶血病的红细胞免疫功能的变化

目的了解新生儿ABO溶血病红细胞免疫功能状态,主要是血清高未结合胆红素对新生儿红细胞免疫功能的影响。方法对48例ABO溶血病新生儿测定血清总胆红素,同时采用郭峰的酵母多糖花环实验法测定血清红细胞C3b补体受体花环(RBC-C3b RR)和红细胞免疫复合物受体花环(RBC-ICR),并与100例正常新生儿对照比较。结果 (1)ABO溶血病患儿的血清RBC-C3b R低于正常新生儿,其RBC-ICR则高于正常新生儿,差异有统计学意义,P﹤0.05;(2)不同水平血清总胆红素的新生儿间比较,RBC-C3b RR无统计学差异,P﹥0.05。RBC-ICR有统计学差异,P﹤0.05;(3)相关分析表明,ABO溶血病患儿的RBC-ICR与血清总胆红素、血清未结合胆红素水平具有相关性,且呈正相关,P﹤0.05;RBC-C3b RR与光疗的时间存在明显相关,呈正相关,P﹤0.05。结论 ABO溶血病患儿的红细胞免疫功能明显低下。     

茵栀黄颗粒辅助头孢唑林钠治疗新生儿感染性黄疸疗效

目的 观察茵栀黄颗粒辅助头孢唑林钠治疗新生儿感染性黄疸疗效。方法 选取2018年4月-2020年6月承德医学院附属医院新生儿科收治的感染性黄疸患儿106例,以简单随机化法分为研究组和对照组,每组53例。常规治疗基础上,对照组给予头孢唑林钠,研究组给予茵栀黄颗粒辅助头孢唑林钠,均治疗1周。比较两组临床疗效及治疗前后行为神经评估量表(NBNA)评分、黄疸指数、血清总胆红素(TBil),心肌酶谱指标[肌酸激酶同功酶(CK-MB)、肌酸激酶(CK)、乳酸脱氢酶(LDH)],炎症反应指标[C反应蛋白(CRP)、白细胞计数(WBC)、降钙素原(PCT)],红细胞免疫功能指标[红细胞免疫复合物花环率(RBC-ICR)、红细胞C3b受体花环率(RBC-C3bRR)]水平。结果 研究组治疗1周后总有效率(92.45%)高于对照组(73.58%)(P<0.05);研究组治疗3 d、1周后NBNA评分高于对照组,黄疸指数、血清TBil水平低于对照组(P<0.05);血清CK-MB、CK、LDH、CRP、WBC、PCT及RBC-ICR水平低于对照组,RBC-C3bRR水平高于对照组(P<0....     

Enhanced T-cell rosette formation in shigellosis by the in vitro use of thymopoietin.

The results from this study suggest that the large nul cell lymphocyte population seen in patients with Shigella dysentery, does contain a sub-population of cells that will respond in vitro to thymopoietin, a bovine thymic extract, by increased E-rosette formation. It is felt that this sub-population is in fact immature T-cells. A previous study has shown that an unusual leukaemoid reaction develops in a substantial number of patients with Shigella dysentery. The leukaemoid response was primarily granulocytic in nature but there was also a substantial increase in the mean number of lymphocytes. The proportion of the various populations of lymphocytes from leukaemoid and non-leukaemoid subjects were altered, B-cells remained constant, while the T-cells were depressed with a corresponding rise in the proportion of nul cells. The cumulative results of this and other studies demonstrate that the T-cell arm of immunity is compromised in shigellosis. Indeed the degree of compromise may ultimately be the decisive factor in determining the severity of this disease.     

Effect of organophosphoric pesticides on the behaviour of NBT-dye reduction and E rosette formation tests in human blood

Summary Investigations have been undertaken to determine the effect of occupational exposure to organophosphoric pesticides on the behaviour of NBT-dye reduction and spontaneous E rosette formation tests. A significant decrease of the values of the examined cellular parameters of human immunity and of the absolute lymphocyte count in peripheral blood was found in 31 workers, as compared to 30 controls. Moreover, the NBT-dye reduction decrease showed a positive linear correlation with a reduced acetylcholinestrase (AChE) activity. The exposure time showed a negative linear correlation of the phagocytic index of the NBT-dye reduction test. It is concluded that depressed NBT-dye reduction and diminished spontaneous E rosette formation may be regarded as a probable mode of the effect of organophosphoric chemicals on metabolic and membrane damage of human cells. A possible role of endogenous acetylcholine poisoning in the development of these pathologic conditions is also discussed.     

The effect of ozone on human cellular and humoral immunity: characterization of T and B lymphocytes by rosette formation.

Eight healthy male subjects were exposed to 784 μg/m³ (0.4 ppm) of ozone for 4 hours in an environmentally controlled exposure chamber. Peripheral blood samples were taken before and after air and ozone exposures and again at 72 hours and 2 weeks. These blood samples were examined for the percentages of rosette-forming T and B lymphocytes. No statistically significant depressions in T-lymphocyte rosette formation with sheep erythrocytes were found following exposure to air or ozone. The ability of B lymphocytes to form rosettes with sensitized human erythrocytes was depressed following ozone exposure. These results suggest that surface receptors and/or the cell membranes of peripheral blood B lymphocytes may be temporarily altered following short-term exposure to ozone.