克山病心肌IgG免疫组化观察

一些学者曾查出克山病的血清免疫复合物阳性,说明克山病可能有免疫病理现象存在。李正茂,郭珍对克山病尸检中的心肌作了免疫荧光的观察,同样查到     

变应性哮喘患者细胞免疫水平的变化

对７４例变应性哮喘患者进行Ｔ细胞总数、功能检测。Ｅ－花环试验：７１例４５％±１．７１％，淋巴细胞转化试验：４５例３６８２０．４０±３２９０．７９ｃｐｍ。两项检查结果均高于正常对照组，淋巴细胞转化有显著差异，（Ｐ＜０．０１）。     

几种肿瘤患者红细胞免疫粘附功能的初步观察

本文报道了30例食管癌、20例胃癌、15例乳腺癌、8例肝癌、10例淋巴瘤等患者红细胞免疫粘附功能的初步结果,并与67例献血员值进行了比较。几种肿瘤病人的RBC C_(3b)RR低下,RBC-ICR较高,两者与献血员值比较都有明显差别(P<0.01),几种肿瘤患者CIC阳性率在60～83.4％之间。对食管癌患者部分病例进行跟踪检测,并对其临床意义进行了初步探讨。     

慢性肺原性心脏病患者血浆内皮素和心钠素水平的观察

本文测定了31例健康人和37例慢性肺原性心脏病(CPHD)患者急性发作期与缓解期血浆内皮素(ET)和心钠素(ANP)水平,以探讨两者之间的关系在CPHD的发生、发展中的病理生理学意义。现报道如下。     

风湿性心脏病细胞免疫指标的检测

风湿性心脏病细胞免疫指标的检测石冠荣，梁茂云，张彦亮，张修平，董波，潘祥林风湿性心脏病（ＲＨＤ）是由Ａ族链球菌感染诱发的自身免疫性疾病，为探讨免疫因素在其发病中的作用与意义，我们检测了ＲＨＤ患者的可溶性白细胞介素２受体（ｓＩＬ－２Ｒ）、自然杀伤细胞（...     

尿毒症血透患者细胞免疫功能观察

本文检测了23例尿毒症血液透析患者外周血PBMC的几项细胞免疫指标,并以健康献血员作为对照组。结果表明血透患者T细胞亚群和ADCC活性与正常对照组差异无显著意义(P>0.05)。T细胞转化功能、NK细胞活性、IL-2和IL-2受体活性均低于正常对照组(P<0.01)。     

支气管哮喘患者红细胞免疫指标及外周血相关细胞因子水平的观察

近年来的研究证实 ,呼吸道炎症发生过程中有红细胞免疫参与 ,且与外周血细胞因子水平关系密切[1,2 ] 。为探讨红细胞免疫指标及相关细胞因子水平与支气管哮喘发作与缓解的关系 ,我们测定了 35例哮喘患者的红细胞Ｃ3 受体花环率 (ＲＢＣＣ3 ｂＲＲ)、外周血白细胞介素 10 (ＩＬ - 10 )及白细胞介素5 (ＩＬ - 5 )的水平。对象和方法一、对象 :哮喘发作期组 35例 (男 2 2 ,女 13) ,年龄 14～ 6 4岁。哮喘缓解期组 30例 (男 2 0 ,女 10 ) ,年龄 12～ 6 9岁 ,为哮喘发作后病情缓解 2周以上者。病情程度判定按 1993年中华医学会呼吸病学会哮喘学组…     

再生障碍性贫血患者细胞免疫指标的观察

采用酶免疫和放射免疫法分析了再生障碍性贫血(AA)患者外周血T淋巴细胞亚群、血清肿瘤坏死因子(TNF)、γ-干扰素(IFN-γ)和IL-2水平,以及患者外周血单个核细胞体外诱生的上述细胞因子水平。结果表明:患者CD4/CD8细胞比值降低或倒置(P<0.001),血清TNF和IFN-γ水平显著增高(P<0.01),外周血单个核细胞诱生的TNF、IFN-γ和IL-2水平均明显高于正常对照(P<0.01)。提示T淋巴细胞亚群比例失调和TNF、IFN-γ等造血负调控因子的过量分泌可能与AA的发病机制有关。     

SLE患者几种细胞因子水平变化的观察

<正>系统性红斑狼疮(SLE)病人存在着一系列细胞因子的异常.而在这些异常的细胞因子之间,是否有内在的联系,是一个令人感兴趣的问题.我们对不同病情的SLE患者的几种细胞因子之间的相互联系作了一些初步的比较观察.1 对象和方法1.1 研究对象 活动期SLE病人系在上海市仁济医院和上海市瑞金医院就医的患者,均为女性,年令18～54岁.符合1982年美国风湿病学会诊断标准.缓解期SLE病人系上述医院门诊随访SLE患者,均为女性,年龄18～54岁.正常对照为年龄与上述两组SLE患者接近的正常女性.     

用单克隆抗体观察慢性肝炎患者细胞免疫状况的变化

本文用多种单克隆抗体对32例慢性肝炎患者的细胞免疫状况进行了研究。结果发现患者肝组织内淋巴细胞和单核、巨噬细胞浸润明显增加,坏死区浸润细胞主要是T_3~+和T_8~+细胞。B淋巴细胞浸润极少,浸润淋巴细胞表面IL—2受体阳性细胞与外周血淋巴细胞表面IL—2受体阳性细胞率均无改变。患者外周血单个核细胞改变为T_3~+下降,T_4/T_8比例下降,单核、巨噬细胞、B淋巴细胞及淋巴细胞表面HLA—DR、DP、DQ标志与正常人无差异。     

传染病患者红细胞免疫粘附功能的初步观察

<正> 红细胞的免疫功能已日益为国内外学者所关注。红细胞表面膜结构中有C_(ab)受体,通过识别C_(3b)或C_(4b)而粘附抗原-抗体-补体复合物,以达到清除循环免疫复合物。而在红细胞免疫功能低下时则可引起免疫复合物在局部组织、脏器中的沉积,造成免疫病理损伤。 测定红细胞免疫功能的方法,主要有红细胞免疫复合物花环试验、红细胞C_(3b)受体花环试验、红细胞膜ELISA、红细胞酶联免疫竞争吸附试验(E-ELISA)、免疫酶或免疫荧光技术测定周围血涂片中红细胞粘附抗原等。目前已知许多传染病的发病机     

六例小儿潜在型克山病心肌活检的电镜观察

Myocardial biopsy from six children with latent Keshan disease was performed. Samples taken from right heart ventricles were prepared and investigated under an H-600 electron microscope. Some samples were also prepared with the lanthanum preparation for studying the membrane permeability of the myocardium. Ultrastructurally, most of the sarcolemma of myocardial cells remained intact, and some occasionally showed local minimal defects. Lanthanum particles are located outside the myocardial cells, and only a small number of them entered into the cells; but they were located between myofibrils and mitochondria. Mitochondria showed some extent of proliferative and degenerative changes. Some of them were swollen, and their cristae resolved and matrix lightly stained. Transverse system, sarcoplasmic reticulum and the perinuclear cisterns were dilated apparently. Myolytic foci could be seen scarcely. Proliferation of collagen fibers could also be found here and there in all the samples. It was showed that certain permeability disorders of the sarcolemma were still existent, although these patients were in a period of compensation and reparation. Perhaps, some pathogenic factors were still acting on them at that time.     

缺血性心脏病患者血浆神经肽Y水平变化

目的：为探讨神经肽Y（NPY）缺血性心脏病发生,发展中的病理生理意义。方法：95例缺血性心脏病患者包括心绞痛者35例（A组）急性心肌梗塞（AMI）无心衰者31例（B组）及AMI伴左心衰者29例（C组）;分别于入院时与入院后1,4,7及28天测定了AMI患者血浆NPY的含量,心绞痛患者入院时与入院后1天血浆NPY水平变化;同时测定了37例正常人血浆NPY的含量。心绞痛患者入院时与入院后1天血浆NPY水平变化;同时了37例正常人血浆NPY的含量。结果：入院时患者血浆NPY水平均高于正常对照组,且病情越重升高越显著。A组;1天后恢复至对照组水平。B组：入院后1天较入院时升高;入院后4－7天逐渐降低,但仍高于对照组;28天后恢复至对照组水平。C组：入院后1,4天较入院时显著升高;其中6例死亡患者入院后4天极度升高。其余23例入院后7天开始下降,仍显著高于入院时的水平;入院后28天较入院时明显降低,但仍明显高于对照组。结论：血浆NPY含量测定对于缺务性心脏病患者病情观察及预后判定具有重要意义。     

慢性乙肝患者抗病毒治疗前后特异性T细胞免疫观察

目的 了解抗病毒治疗前后慢性乙型肝炎患者特异性T淋巴细胞对HBV抗原蛋白免疫应答的变化及其特征.方法 收集17例慢性乙型肝炎患者抗病毒治疗前及治疗后1个月、3个月的外周血单个核细胞,以HBV特异性抗原蛋白HBsAg、HBcAg和HBeAg为刺激物,酶联免疫斑点法检测其分泌IFN-γ产生斑点的情况.同时对血清HBV DNA和HBsAg、HBeAg等病毒学指标及谷丙转氨酶(ALT)等生化学指标进行榆测并分析其相关性.结果 治疗前,所有患者ALT、总胆红素(TBiL)均高于正常上限,17例患者HBV DNA均大于104拷贝/ml;治疗1个月后,ALT复常率为35.3%,9例患者HBV DNA降为检测下限以下;治疗3个月后,ALT复常率为58.8%,有11例患者HBV DNA降为检测下限以下.抗病毒治疗前、治疗1个月、治疗3个月患者针对HBV特异性蛋白总的T细胞反应阳性率分别为64.7%、76.5%和82.4%,其差别无统计学意义.不论治疗前后,患者对HBeAg的特异性T细胞反应频率和平均反应强度最高;治疗后,对3种蛋白的特异性T细胞反应频率和平均反应强度各有不同程度的增加,其中以对HBcAg蛋白的平均反应强度的增强最明显,治疗前和治疗3个月,治疗1个月和治疗3个月之间的差别都有统计学意义.患者对HBcAg蛋白的特异性T细胞反应平均反应强度与病毒载量有明娃负相关,与血清ALT无明显相关性.结论 本研究结果提示抗病毒治疗后,患者对HBV的特异性T细胞免疫应答有所增强,这种改变可能与HBV DNA的下降有关,检测HBV特异性T细胞反应对丁解患者的免疫状态有重要的意义.

Abstract：

Objective To explore the responses of antigen-specific T cells stimulated by hepatitis B virus(HBV)-specific proteins in chronic hepatitis B patients accepting antiviral therapy. Methods Seventeen patients with chronic hepatitis B (CHB) accepting antiviral therapy were included in this study. The peripheral blood monocular cell ( PBMC) were separated from the whole blood collected at the three different time of before and one and three months after accepting antiviral therapy. ELISPOT assay was used to detect the frequency and strength of secreting IFN-γ cells of PBMC stimulated by HBsAg, HBcAg and HBeAg. HBV virus loading, HBsAg, HBeAg, ALT and AST in serum were detected at the same time. Results After three months therapy, ALT, TBiL were improved in all patients, and HBV DNA level were dropped and undetectable in 11 cases. The rates of T cell response in patients to HBV specific proteins were 64. 7% , 76. 5% and 82. 4% at the time of before and one and three months after accepting antiviral therapy, respectively. The frequency of responses of antigen-specific T cells stimulated by HBcAg was higher than that stimulated by HBsAg or HBeAg, and the frequency was enhanced after antiviral therapy. The average response magnitude was expressed as spot forming cells (SFC) per million input cells. SFC of T cell responses to HBcAg was also higher than to HBsAg or HBeAg. There was no significant difference in SFC of T cell responses to HBsAg or HBeAg at the time of before and after antiviral therapy, but there were significant difference in SFC of T cell responses to HBcAg at the time of before and after antiviral therapy. SFC of T cell responses to HBcAg was negatively associated with HBV DNA, and no associated with level of ALT in serum. Conclusion The responses of antigen-specific T cells were improved in CHB patients accepting antiviral therapy which associated with the decrease of HBV DNA. It suggested to investigate HBV specific T cell responses was important.     

心血管疾病中TNF等细胞因子水平的观察

应用双抗体夹心法检测44例心血管疾病（其中冠心病26例,风心病10例,高血压病8例）患者血清肿瘤坏死因子（TNF）、白细胞介素6（IL-6）和白细胞介素8（IL-8）水平。结果显示,心血管病患者血清TNF、IL-6和IL-8水平均显著高于正常人（P<0.002,<0.005和<0.05,不同心血管疾病间TNF、IL-6和IL-8水平无显著性差异（P>0.05）,INF与IL-6和IL-8及IL-6与IL-8之间均成正相关（r分别为0.546、0.394和0.606,P为<0.001、<0.01和<0.001）。提示TNF、IL-6和IL-8可能参与某些心血管疾病的发病及病理变化过程。     

风湿性心脏病心脏瓣膜置换术后细胞免疫与心肌酶谱水平、心功能的关系分析

为探讨风湿性心脏病(简称风心病)患者心脏瓣膜置换术后细胞免疫与心肌酶谱水平、心功能的关系,对52例接受心脏瓣膜置换术的风心病患者的临床资料进行回顾性分析.结果 显示,风心病患者淋巴细胞百分比、CD4+/CD8+均于术后1d下降(P＜0.05),且术后7、14d均逐渐恢复(P＜0.05);心肌酶谱[肌酸激酶(creati...     

先天性心脏病患者B19等病原感染的调查研究

Objective　To explore relationship of Parvovirus B19(B19), Toxoplasma gondii(TOX), rubella virus(RV), cytomegalovirus (CMV) or herpes simplex virus type-2 (HSV-2) infection with congenital heart disease (CHD). Methods　We conducted a case-control study on detection of B19, TOX, RV, CMV or HSV-2 gene in cardiac tissue of 66 cases of CHD and 38 cases controls with polymerase chain reaction (PCR). Results　1) The positive rates of B19, TOX, CMV and HSV-2 in 66 cases CHD were 18.2%, 15.2%, 25.8% and 4.5%, respectively, while that in 38 cases control groups were 0%, 2.6%, 21.2% and 2.6%, reapectively. The positively of B19, TOX was significantly different (P<0.05) between CHD and control groups, while no statistical difference (P<0.05) for CMV and HSV-2. 2) Seven of 30 (23.3%) CHD were positive for RV-RNA, compared with control group which was all negative (P=0.0327). Conclusion　The results show that B19, TOX, RV infection mighet be important risk factors for CHD, while CMV and HSV-2 had no relationship with CHD.     

IL-1β表达与食管癌患者细胞免疫水平的相关性分析

目的 探讨IL-1β表达与食管癌患者细胞免疫水平的相关性.方法 收集38例食管癌患者外周血标本,流式细胞仪检测血清中IL-1β的水平及CD4+T/CD8+T细胞比值;收集8例PD-1免疫治疗前后食管癌患者外周血标本,采用酶联免疫吸附法检测血清中IL-1β的水平;利用TIMER数据库分析IL-1β表达与免疫细胞浸润及关键调控基因的关系.结果 外周血中IL-1β的水平和CD4+T/CD8+T细胞比值呈负相关(P=0.047);血清中IL-1β的水平在PD-1单抗免疫治疗后显著下降(P=0.038);食管癌组织中IL-1β的表达与巨噬细胞浸润呈负相关(P=0.00241),与中性粒细胞浸润呈正相关(P=0.00324);食管癌组织中IL-1β表达与B2M(P=0.0128)和PD-L1(P=0.00174)的表达呈正相关,与TNFRSF13C的表达呈负相关(P=0.0094).结论 IL-1β的表达与食管癌患者细胞免疫水平呈负相关,其机制与抑制免疫细胞浸润有关.     

潜在型克山病的血液流变学变化

克山病患者存在血液流变学指标的改变，在克山病模型中报道较多，在克山病患者中报道较少．本文对宁县于午岭林区克山病重灾区某林场职工进行普查诊断的潜在型克山病患者32例，进行了血液流变学检测。现报告如下：资料和方法1病例选择对宁县子午岭林区克山病重灾区某林场职工进行普查筛选，对象均为长期居住于病区的居民，根据（克山病防治工作标准》执行。全部病例进行了详细体检，心电图及X线检查。共筛选出克山病48例。再进行超声心动图及心功能测定，除外伴有心功能不全者后，共确诊潜在型克山病32例。其中男14例，女18例。年龄30～56…