针刺对痛经大鼠神经-内分泌影响的机制初探

目的:探讨针刺对痛经大鼠下丘脑-垂体-卵巢功能活动的影响。方法:雌性Wistar大鼠随机分为正常组、模型组、针刺组、益母草膏组,每组10只,己烯雌酚灌胃12 d建立痛经模型。针刺组取"关元",双侧"地机"三阴交"穴,留针30 min;益母草膏组采用益母草膏(每日80 g/kg)灌胃治疗。两组均在灌服己烯雌酚的第5天开始治疗,每日1次,连续7 d。末次给予己烯雌酚1 h后,腹腔注射催产素,观察各组大鼠的扭体反应潜伏期和30 min内扭体数;放射免疫法检测血清促卵泡生成素(FSH)、促黄体生成素(LH)、雌二醇(E2)、孕酮(P)的水平;RT-PCR检测各组大鼠下丘脑、卵巢促性腺激素释放激素(GnRH),垂体促性腺激素释放激素受体(GnRH-R),子宫雌激素受体(ER)及孕激素受体(PR)mRNA表达的差异。结果:①针刺组和益母草膏组的大鼠扭体次数明显低于模型组,潜伏期高于模型组(P<0.05);②针刺组和益母草膏组与模型组比较,FSH、LH、E2含量显著降低(P<0.05),P含量明显升高(P<0.05);③针刺组和益母草膏组下丘脑、卵巢GnRH,垂体GnRH-R,子宫ER mRNA表达水平均低于模型组(P<0.05),子宫PR mRNA表达水平高于模型组(P<0.05)。结论:针刺治疗原发性痛经的神经-内分泌机制可能与针刺调节大鼠下丘脑-垂体-卵巢轴的性激素及其受体表达有关。     

逆针灸关元对去卵巢大鼠下丘脑-垂体-卵巢轴影响

目的观察逆针灸“关元”对去卵巢大鼠的影响,探讨其调节围绝经期综合征下丘脑-垂体-卵巢(HPO)轴的机制。方法将3月龄SD雌性大鼠48只随机分为正常组、假手术组、去卵巢组、逆针关元组、逆灸关元组。术前30 d给逆针关元组和逆灸关元组予以针刺或艾灸,随后制作去卵巢大鼠模型,除正常组和假手术组外,其余组均行去卵巢手术。采用酶联免疫法测定大鼠下丘脑、垂体及子宫的促性腺激素释放激素(GnRH)、卵泡刺激素(FSH)、促黄体生成激素(LH)、雌二醇(E2)、雌激素受体(ER)的水平变化。结果与正常组比较,模型组E2、ER和GnRH水平显著降低(P＜0.01),FSH和LH的水平显著升高(P＜0.01)。与模型组比较,逆针和逆灸组E2、ER和GnRH水平均不同程度升高(P＜0.05),FSH和LH水平不同程度降低(P＜0.05)。逆针组和逆灸组比较,组间差异无统计学意义(P＞0.05)。结论逆针和逆灸“关元”均能良性地调节去卵巢大鼠HPO轴的紊乱,其对HPO轴的作用强度基本相同。     

更年乐颗粒剂对12月龄大鼠下丘脑-垂体-卵巢轴的调节作用

目的:通过检测内分泌激素的水平,下丘脑雌激素受体(ER)表达,考察更年乐对下丘脑-垂体-卵巢轴(HPO)的调节作用。方法:采用12-15月龄SD大鼠,同时设青年鼠作对照,灌胃给药30 d后,放免法检测血清E2、P、Te、FSH、LH水平,免疫组化法检测下丘脑GnRH,垂体和下丘脑ER表达。结果:与更年组比,12月龄组可以升高12月龄大鼠血清中E2,P(P<0.05或P<0,01)水平,有降低LH水平的趋势(P>0.05),明显降低下丘脑GnRH的表达(P<0.05),上调下丘脑ER表达。结论:更年乐能改善因体内雌激素水平降低导致的下丘脑、垂体功能的相对亢进状态,上调下丘脑ER表达,增强雌激素效应,调节HPO轴的失调。     

针刺对下丘脑-垂体-肾上腺轴的调整作用

下丘脑-垂体-肾上腺轴是机体应对外界各种急性或慢性应激的反应中枢,其最高反应中枢是下丘脑室旁核,HPA轴的激活除需应激刺激外,还受到中枢核团的调节作用,随之分泌CRH、ACTH、CORT 3种激素,使机体做出适应性的反应,从而保持内环境的稳定。但机体长期受到应激刺激则使HPA轴处于异常状态,CRH、ACTH、CORT 3种激素分泌异常,机体出现相应的疾病。目前研究发现,针灸能够使CRH、ACTH、CORT 3种激素恢复到正常水平,调整HPA轴功能,但其作用究竟是通过下丘脑室旁核及垂体、肾上腺的作用,还是通过脑内其他中枢核团的调节起效,其内在机制需要今后研究进一步明确。     

功血宁对下丘脑-垂体-卵巢轴功能的影响

目的 :观察功血宁对下丘脑 -垂体 -卵巢轴功能的影响。方法 :以未成年雌性大鼠为对象 ,采用放免方法检测功血宁大、小剂量组、阳性对照组和空白对照组大鼠血清 FSH、L H、E2 和 P水平 ;各组大鼠卵巢、子宫称重 ,进行组织形态学观察及卵泡成熟总数测定 ,并作比较。结果 :功血宁大剂量血清 FSH、L H、E2 水平显著增高 ,卵巢和子宫重量明显增重 ,卵泡成熟总数增加 ,组织形态学观察有明显改变。结论 :功血宁能提高大鼠生殖激素水平 ,改善卵巢功能 ,促进排卵。说明功血宁对下丘脑 -垂体 -卵巢轴功能有整体调节作用。     

中药复方对下丘脑-垂体-卵巢轴作用机理实验研究概况

目的：综述中药复方对下丘脑-垂体-卵巢轴的作用机理。方法：通过中药复方的实验研究，对下丘脑-垂体-卵巢轴的各个环节的影响进行综合分析。结果：中药复方可调节紊乱的植物神经功能、生殖内分泌系统和免疫功能，从而对女性神经内分泌免疫系统发挥双向调节作用。结论：脑（心）肾-冲任-胞宫轴与大脑皮层-下丘脑-垂体-卵巢轴相对应，为中医药治疗、研究妇科疾病提供理论依据。     

电针对围绝经期抑郁症大鼠下丘脑-垂体-卵巢轴的影响

目的:观察电针对围绝经期抑郁症大鼠下丘脑-垂体-卵巢(HPO)轴激素及血清β-内啡肽(β-EP)的影响,探讨针刺调节围绝经期抑郁症大鼠的神经内分泌机制。方法:雌性SD大鼠60只,随机分为空白组、假手术组、模型组、西药组和电针组,采用双侧卵巢切除和慢性不可预见刺激结合建立围绝经期抑郁症大鼠模型。电针组电针"百会"、双侧"肾俞"、双侧"三阴交",西药组使用盐酸氯米帕明灌胃,均每日治疗1次,连续治疗28d。观察大鼠阴道涂片、糖水偏嗜率,运用酶联免疫吸附法测定血清雌二醇(E2)、黄体生成素(LH)、促性腺激素释放激素(GnRH)、β-EP的含量。结果:与空白组相比,模型组及假手术组糖水偏嗜率明显减小(P0.05);与模型组相比,电针组及西药组糖水偏嗜率升高(P0.05)。模型组血清E2、β-EP含量较空白组和假手术组明显降低(P0.05),LH、GnRH明显升高(P0.05);与模型组比较,电针组及西药组血清E2、β-EP含量明显升高(P0.05),LH、GnRH明显降低(P0.05)。结论:针刺能够良性调节围绝经期抑郁症大鼠紊乱的HPO轴功能,改善围绝经期抑郁症状。     

电针“三阴交”对自然围绝经期大鼠下丘脑-垂体-卵巢轴的影响

目的:观察电针"三阴交"对自然围绝经期大鼠的影响,探讨其对下丘脑-垂体-卵巢(HPO)轴紊乱的调节机制。方法:将3月龄SD雌性大鼠8只作为正常组,11~15月龄SD雌性大鼠阴道涂片法筛选符合围绝经期综合征表现者16只,随机分为模型组和电针组,各8只。电针组取两侧"三阴交"穴做电针,每次20min,隔日1次,共治疗15次。采用酶联免疫法测定各组大鼠下丘脑中促性腺激素释放激素(GnRH),垂体中卵泡刺激素(FSH)、促黄体生成素(LH)和卵巢中雌二醇(E2)的含量。结果:与正常组相比,模型组E2水平显著降低(P0.01),FSH、LH、GnRH水平均显著升高(P0.01);与模型组相比,电针组E2水平明显升高(P0.05),FSH、LH和GnRH含量均有不同程度下降(P0.05)。结论:电针"三阴交"能够对围绝经期大鼠紊乱的HPO轴功能起到良性调节作用。     

循经通络推拿结合药物对乳腺增生大鼠下丘脑-垂体-卵巢轴的影响

目的 观察循经通络推拿手法结合药物治疗与单纯手法、单纯药物治疗对乳腺增生(MGH)大鼠的疗效差异及对下丘脑-垂体-卵巢(HPO)轴的影响。方法 SD大鼠随机分为对照组、模型组、手法组、药物组、药物加手法组,每组10只。采用孕雌激素联合造模法制备乳腺增生症模型。手法组予以循经通络推拿手法治疗,药物组灌胃给予三苯氧胺,药物加手法组同时给予循经通络推拿和药物灌胃。治疗结束后测量大鼠乳头直径和乳头高度,HE染色观察乳腺组织病理学变化,ELISA法检测血清中卵泡刺激素(FSH)和黄体生成素(LH)水平,Western-blot及RT-RCR检测下丘脑促性腺激素释放激素(GnRH)及垂体促性腺激素释放激素受体(GnRH-R)的蛋白和 mRNA 表达。结果 与模型组比较,各治疗组大鼠乳头直径、乳头高度显著减小(P<0.05);药物加手法组大鼠乳腺组织病理形态明显改善,手法组和药物组大鼠乳腺组织形态改善不明显;药物组、药物加手法组血清FSH水平明显降低,LH水平显著升高(P<0.05),手法组大鼠血清FSH、LH水平变化不明显(P>0.05);药物加手法组大鼠GnRH、GnRH-R的...     

补肾方药对下丘脑-垂体-卵巢轴作用的研究概述

中医学认为肾藏精,主生长发育与生殖,在机体的整个生命活动中起着主导作用,决定着人的生长衰老的全过程.对妇女来说,肾对生殖功能的调节是通过肾-天癸-冲任-胞宫轴而进行的,肾主宰着"肾-天癸-冲任-胞宫"之间的协调.     

EFFECTS OF ACUPUNCTURE ON THE HYPOTHALAMUS-PITUITARY-GONADAL(HPG) AXIS OF THE AGED RATS

Recentstudieshavedemonstratedthatacupuncturecouldregulatethefunctionsofhy-pothalamuffepitutary-gonadalaxis,exerttheef-fectsonthereleaseoftestosterone(T),estradiol(Ez),luteinizinghormone(LH),folliclestimulat-inghormone(FSH),luteinizinghormonere-leasignhorm…     

AN ANALYSIS ON THE THERAPEUTIC EFFECT OF ACUPUNCTURE AT SANYINJIAO POINT FOR TREATMENT OF 120 CASES OF PRIMARY DYSMENORRHEA

In the present paper, the authors make an analysis on the therapeutic effect of acupunc-ture at Sanyinjiao(SP 6) alone for treatment of 120 cases of primary dysmenorrhea. Results show atotal effective rate of 96.7%.     

ACUPUNCTURE TREATMENT OF 26 CASES OF PRIMARY DYSMENORRHEA

Theauthorsofthepresentpaperadoptedacupuncturetherapytotreat26casesofdysmen-orrheaandachievedsatisfactoryresults.Itisre-portedasfollows:GENERALDATAAllthe26casesofdysmenorrheawereuptothestandardsfordiagnosisofprimarydysmenor-rhearecordedintheteachingmateria…     

沉默穴位Cx43的表达后针刺镇痛的实验研究

目的：研究沉默痛经大鼠穴位局部Cx43的表达对针刺效应的影响和针刺治疗原发性痛经大鼠的镇痛机制。方法：利用己烯此酚造成原发性痛经的大鼠模型，应用RNAi技术沉默穴位局部Cx43的表达。将50只雌性大鼠随机平均分为5组：正常组（对照），模型组（己烯雌酚），针刺组（己烯雌酚＋针刺），针刺＋干扰组（己烯雌酚＋针刺＋pSilencer—Cx43-shRNA）和针刺＋干扰对照组（己烯雌酚＋针刺＋pSilencer—Con—shRNA）。末次注射催产素后，观察痛经大鼠30min的扭体数。半定量RT—PCR和免疫组织化学检测大鼠子宫内膜催产素（OTR）和加压素（VPR）mRNA和蛋白的表达。结果：①干扰组穴位处的Cx43显著低于针刺组（P〈0．05），针刺组和干扰对照组之间无明显差别（P〉0．05）。②与模型组、干扰组比较，针刺组能显著延长痛经大鼠的扭体潜伏期，减少扭体数。③与正常组比较，模型组OTR和VPR mRNA和蛋白水平显著增高（P〈0．05）。与模型组比较，针刺组和干扰对照组的OTR和VPR mRNA和蛋白水平显著降低（P〈0．05）。模型组和干扰组之间OTR和VPR mRNA和蛋白水平无显著性差异（P〉0．05）。结论：局部注射Cx43特异性shRNA真核表达载体有效地沉默了穴位Cx43的表达，并显著地影响了针刺效应，表明Cx43可能与针刺疗效有密切关系。针刺通过调节子宫内膜OTR和VPR的表达来有效治疗原发性痛经．     

EFFECTS OF DIFFERENT QUANTITY OF ACUPUNCTURE STIMULUS ON UTERINE MICROCIRCULATION IN DYSMENORRHEA RATS WITH COLD SYNDROME AT DIFFERENT TIMEPOINTS

Objective: To observe effects of different quantity of acupuncture stimulus on uterine microcirculation in dysmenorrhea rats with cold syndrome at different time-points, and to discuss the relationship between quantity of acupuncture stimulus and acupuncture effect.Methods: Female dioestrus rats were randomly divided into4 groups with 8 rats in each group.Rats of the model control group and the stimulus groups were frozen in a-25 ℃ freezer for 5 d, 4 h per day, while subcutaneous estradiol benzoate injection was given for 10 d, with intra-peritoneal injection of oxytocin,2 U/each, 1 h after the last injection.On the 10 th day, the stimulus groups were given different quantity of acupuncture stimulus on both sides of Sanyinjiao(SP6) for 20 min.Results: Compared to the saline control group, diameters of microvessel of the model control group at all time-points and diameters of capillary(except 40 and 60 min) were significantly decreased(P0.01), numbers of capillary were reduced significantly(P0.01), clarity of microvessel was worse with color getting darker, and blood flow was slower or stagnated(P0.01 or P0.05).Compared to the model control group, diameters of microvessel of the A stimuli group at all time-points, and diameters of capillary(only 5, 10, 20 and 30 min) were significantly increased(P0.01 or P0.05), clarity of microvessel(except 20 min) was better, color was(only 10, 20, 40 and 50 min) redder, and blood flow at all time-points was speeded up significantly(P0.01); diameters of microvessel of the B stimuli group were increased only at 30 min(P0.01), and clarity of microvessel(only 50 and 60 min) was better(P0.05).Compared to the B stimuli group, diameters of microvessel of the A stimuli group at all time-points and diameters of capillary(only 5, 10, 20 and 30 min) were significantly increased(P0.01 or P0.05), clarity of microvessel(only 30 and 40 min),color(only 5, 20 and 40 min) and blood flow(except 50 and 60 min) were significantly improved(P0.01 or P0.05).Conclusion: Acupuncture could improve uterine microcirculation.The effects of different quantity of acupuncture stimulus on uterine microcirculation were different, and the effect in A stimuli group was better.     

EFFECT OF ACUPUNCTURE ON PLASMA cAMP AND cGMP IN RATS WITH ACUTE CEREBRAL INFARCTION

Ａｃｕｔｅｃｅｒｅｂｒａｌｉｎｆａｒｃｔｉｏｎｉｓｏｎｅｏｆｔｈｅｍｏｓｔｃｏｍｍｏｎｌｙｅｎｃｏｕｎｔｅｒｅｄｃｅｒｅｂｒｏｖａｓｃｕｌａｒｄｉｓｅａｓｅｓ,ａｃｃｃｏｕｎｔｉｎｇｆｏｒ75%[1].Ｒｅｓｅｎｔｙｅａｒｓ’ｒｅｓｅａｒｃｈｅｓｉｎｄｉｃａｔｅｔｈａｔｔｈｅｃｏｎｔｅｎｔｓｏｆｃｙｃｌｉｃｎｕｃｌｅｏｔｉｄｅｓｉｎｐｌａｓｍａａｎｄｃｅｒｅｂｒｏｓｐｉｎａｌｆｌｕｉｄｃｈａｎｇｅｏｂｖｉｏｕｓｌｙｗｈｅｎｄｉｓｏｒｄｅｒｓｏｆｃｅｎｔｒａｌｎｅｒｖｏｕｓｓｙｓｔｅｍｏｃｃｕｒ.Ｔｈｉｓ…     

STUDY OF ACUPUNCTURE EFFECT ON NATURAL CYTOTOXICITY IN RATS

When the effects of acupuncture were compared with those of naloxone (Nx)and the control in rats,the immunoreactivity (IR) of substance P (SP),5-hydoxytryptamine (5HT),vasoactive intestinal peptide (VIP)and somatostatin (SOM),natural cytotoxicity,α-naphthylacetate esterase-focal pattern (ANAE-F) subpopulation and the ratio of cGMP-IR/cAMP-IR in thecirculating lymphocytes were elevated.After incubation of the lymphocytes in vitro with exogenousligends:SP,5-HT,VIP and SOM,an enhanced effect on their respective receptors,a dual regula-tion effect on NC were exhibited.The increase or decrease in NC depended on the basic level of NCprior to incubation.Both the acupuncture effect and the above incubation effect could be inhibited bynaloxone.The way in which acupuncture affected NC is discussed in this paper.