14-3-3β基因与胃癌淋巴结转移的相关性研究

目的：探讨敲减及过表达14-3-3β基因对胃癌细胞生物学行为的影响及临床病理改变。方法在 SGC 7901、MGC 803和 HGC 27胃癌细胞系中，利用 RNA 干扰技术抑制14-3-3β基因的表达，分别用 MTT 法、基底膜侵袭实验检测胃癌细胞的增殖和侵袭能力的变化。结果在 HGC27和 MGC803细胞系中，14-3-3β基因的缺失会影响细胞增殖（ P 值分别为0.000、0.000、0.000、0.000、0.000、0.001、0.000、0.005），14-3-3β基因表达缺失的MGC803胃癌细胞侵袭能力降低（ P 值分别为0.000、0.000）。结论14-3-3β基因参与了胃癌细胞增殖和侵袭。     

结肠癌根治术淋巴结廓清范围的探讨

左、右侧结肠癌根治术淋巴结的廓清范围,在国内目前仍有意见分歧。但根据结肠癌的淋巴结转移规律与生物学特性,指导结肠癌的术中淋巴结的廓清,近年通过在中国医大癌研所学习,结合文献,参照日本大肠癌规约的标准,1993年以来,我院共收治不同部位的结肠癌12例,均做R_3半结肠癌切除术,术中廓清主干淋巴结,术后根据病理情况做初步讨论。现报告如下。     

FDGPET能探测淋巴结转移的性质吗？黑素瘤临床研究

此研究目的是确立 FDGPET诊断淋巴结转移的准确性和测定病变最小可探测体积。方法　作者在手术前用 FDGPET研究了临床或仪器诊断为黑素瘤淋巴结转移的 38例患者 5 6个骨盆淋巴结。所有骨盆淋巴结作了解剖 ,FDGPET结果与术后病理组织学结果作了对照 ,静脉注射 FDG平均强度 496兆贝克勒尔 (范围 ,36 6～ 6 99兆贝克勒尔 )后 ,用 GE40 96 WB扫描仪获得PET显像。结果　 FDGPET诊断骨盆淋巴结累及的功效是好的 ,敏感性为 95 % (35 / 37) ,特异性为 84% (16 / 19) ,准确性为91% (5 1/ 5 6 ) ,阳性预测值为 92 % (35 / 38)和阴性预测…     

结肠癌细胞表达的细胞外基质Reelin在淋巴结转移中的作用

探讨一种由结肠癌细胞表达的细胞外基质Reelin在淋巴结转移中的作用.构建了99例结肠癌及其癌旁组织的组织芯片进行免疫组织化学染色,探测其表达强度并结合临床资料进行分析.结果显示,Duke's A,B,C,D分期与结肠癌细胞Reelin表达强度无关,结肠癌细胞Reelin表达强度与其淋巴结转移潜能相关,结肠癌细胞Reelin表达强度每增加一个单位,其转移进入淋巴结的能力将增加3.523倍.结肠癌细胞表达Reelin与其淋巴结转移密切相关,Reelin可以作为结肠癌淋巴结转移预测的一个新指标.     

VEGF-D的表达与结肠癌淋巴管生成及淋巴结转移的相关性

目的探讨血管内皮生长因子-D(Vascular endothelial growth factorD,VEGF-D)在结肠癌组织中的表达规律,及其与结肠癌淋巴管密度(LVD)、淋巴结转移之间的关系。方法采用免疫组化法对43例人结肠癌组织和14例结肠良性病变组织的VEGF-D表达进行分析,以LYVE-1标记淋巴管,测算淋巴管密度,统计分析结肠癌中的VEGF-D表达与结肠癌临床病理特征的关系。采用Western-blot法检测16例人结肠癌组织中淋巴结转移组和无淋巴结转移组的VEGF-D蛋白表达。结果免疫组化结果显示结肠癌组织中VEGF-D的阳性率明显高于结肠良性病变组织,并且与结肠癌组织的LVD成正相关。Western-blot结果显示结肠癌中淋巴结转移组的VEGF-D的表达量较无淋巴结转移组明显增加,VEGF-D表达与患者的肿瘤浸润深度、淋巴结转移和Dukes分期显著相关,而与年龄和肿瘤大小无关。结论结肠癌组织中VEGF-D的高表达可能与结肠癌的淋巴管的新生及淋巴结转移有关。     

磁性碳纳米管载附5-氟尿嘧啶对结肠癌淋巴结转移的靶向抑制作用☆

背景：将碳纳米管制备成磁靶向药物载体后,在磁场作用下能更好地把药物运送至体内靶器官或靶组织。 目的：观察磁性碳纳米管新化疗载体对结肠癌淋巴结转移的抑制效果。 方法：MTT法检测5-氟尿嘧啶、磁性多壁碳纳米管-5-氟尿嘧啶、磁性多壁碳纳米管(每种药物分别含0.000 3,0.003,0.03,0.3,3 g/L 5-氟尿嘧啶)对结肠癌SW480细胞的抑制作用。将含相同浓度5-氟尿嘧啶的5-氟尿嘧啶、磁性多壁碳纳米管-5-氟尿嘧啶、磁性多壁碳纳米管分散液分别注入SD大鼠足垫皮下及结肠癌淋巴结转移裸鼠体内。 结果与结论：体外各质量浓度的5-氟尿嘧啶和磁性多壁碳纳米管-5-氟尿嘧啶对癌细胞的毒性存在剂量依赖性,相同5-氟尿嘧啶质量浓度时两者对体外SW480细胞的抑制作用无明显差异,说明磁性多壁碳纳米管-5-氟尿嘧啶的主要药效成分为5-氟尿嘧啶。体内研究高效液相检测显示磁性多壁碳纳米管-5-氟尿嘧啶能有效聚集在淋巴结,长时间持久释放,淋巴结浓集效果明显优于5-氟尿嘧啶(P < 0.05),且不良反应小,肉眼容易辨识,细胞穿透性好;TUNNEL检测见磁性多壁碳纳米管-5-氟尿嘧啶化疗后结肠癌淋巴结转移灶细胞有明显凋亡现象,在磁场作用下效果更显著。说明磁性多壁碳纳米管-5-氟尿嘧啶对结肠癌SW480细胞淋巴结转移有明显抑制作用。关键词：磁性碳纳米管;5-氟尿嘧啶;淋巴化疗;结肠癌;淋巴结转移;生物材料与纳米技术 缩略语注释：5-FU：5-fluorouracil,5-氟尿嘧啶;mMWNTs：multi walled carbon nanotubes,磁性多壁碳纳米管 doi:10.3969/j.issn.1673-8225.2012.16.008      

卵巢癌淋巴结转移的解剖基础与临床意义

卵巢癌淋巴结转移发生率高,有其自身的特殊规律.本文拟介绍卵巢癌腹膜后淋巴转移的解剖学基础及一些分子生物学机制,及其诊断和治疗中的一些新趋势.     

胃癌转移淋巴结分型的病理研究

胃癌组织学生长方式,是估计胃癌手术预后的重要因素。本组淋巴结转移癌的生长方式,多数(65.4%)与原发癌一致,这一部分转移淋巴结,同原发癌一样可反映胃癌的预后。少数(34.6%)与原发癌生长方式不同的转移淋巴结,总的趋向变恶,推测它们将比原发癌对预后表达得更深刻。在粘液组化分型中,淋巴结转移癌与原发癌的各型分布近似。     

盆腔淋巴结转移与宫颈癌临床病理特征的关系研究

目的 研究宫颈癌临床病理特征与盆腔淋巴结转移的关系.方法 回顾性分析从2013年5月至2015年5月于菏泽市立医院手术治疗且病例资料完整的宫颈癌患者100例,分析其肿瘤的期别、组织学类型、生长类型和盆腔淋巴结转移情况.结果 100例患者中有盆腔淋巴结转移者18例,转移率为18％.18例中有绝经期8例,占8/18 (44.4％),非绝经期12例,占10/18(55.6％),数据差异有统计学意义;高危HPV阳性18例,占100％,阴性0例,数据差异有统计学意义;Ⅰ b11例,占11/18(61.1％),Ⅱa有7例,占7/18(38.1％),差异有统计学意义(P＜0.05);肿瘤≤4cm有14例,占14/18(77.8％),肿瘤＞ 4cm有4例,占4/18(22.2％),差异有统计学意义.(P＜0.05);接受化疗的患者有5例,占5/18(27.8％),未接受化疗的有13例,占13/18(72.2％),差异有统计学意义(P＜0.05).而盆腔淋巴结转移与年龄、病理类型、组织学分级差异无统计学意义(P＞0.05).结论 盆腔淋巴结转移和宫颈癌与高危HPV、临床分期、术前化疗、肿瘤大小有关;且与年龄、病理类型、组织学分级无关.盆腔淋巴结状态结合临床病理因素在临床上个体化治疗具有意义.     

Integrin α4 Induces Lymphangiogenesis and Metastasis via Upregulation of VEGF‐C in Human Colon Cancer

Vascular endothelial growth factor‐C (VEGF‐C) is a key regulator in lymphangiogenesis, and is overexpressed in various malignancies. Integrin α4β1, a new member of the VEGF‐C/VEGF receptor pathway, was found to be overexpressed in melanoma tumors. However, little is known regarding the potential role of integrin α4β1 in lymphangiogenesis and other solid tumors. The aim of this study was to investigate the expression patterns of integrin α4 and VEGF‐C in relation to lymphangiogenesis and clinicopathological parameters in human colon cancer. The expression of integrin α4, VEGF‐C, and VEGFR‐3 was assessed in 71 human colon cancer tissues and 30 paracancerous normal tissues by immunohistochemical staining. Lymphatic microvessel density (LMVD) was measured after D2‐40‐labeling, and the correlations among different factors were statistically analyzed. The expression of integrin α4, VEGF‐C, VEGFR‐3, and LMVD was higher in colon cancer tissues compared with the normal paracancerous colon tissues. There was a positive correlation between the expression of integrin α4 and VEGF‐C. Integrin α4 and VEGF‐C were significantly associated with the clinicopathological parameters (LMVD, Duke's stage, and lymph node metastasis). Kaplan–Meier analyses indicated that patients with high integrin α4 or VEGF‐C expression had significantly shorter overall survival and tumor‐free survival time. Multivariate analyses suggested that integrin α4 and VEGF‐C may serve as independent prognostic factors for human colon cancer. Both integrin α4 and VEGF‐C are involved in lymphangiogenesis and lymphatic metastasis. Our results demonstrated that integrin α4 is a novel prognostic indicator for human colon cancer. Anat Rec, 299:741–747, 2016. © 2016 Wiley Periodicals, Inc.     

经支气管针吸活检术对肺癌伴纵隔淋巴结转移的临床诊断价值

目的评价经支气管针吸活检术(transbronchial needle aspiration,TBNA)对伴有纵膈淋巴结转移的肺癌临床诊断价值。方法对肺部CT检查疑诊为伴有淋巴结转移的肺癌53例患者,在常规支气管镜行刷检和活检后,对转移肿大的纵隔淋巴结行经支气管针吸活检术(TBNA),对获取的标本实行相应的病理学检查。结果 53例患者经三种结合的纤支镜检查明确诊断46例,其中组织活检、刷检和TBNA的阳性率分别是64.2%、60.4%、58.5%,常规组织刷检及活检的阳性率为69.8%,结合TBNA术的阳性率增加至86.8%。参检者术中及术后均未发现严重的并发症。结论 TBNA术对于伴有纵膈淋巴结转移的肺癌患者具有较高的临床诊断价值。     

CT引导下125I粒子瘤内植入治疗腹膜后转移淋巴结的临床应用价值

目的 探讨CT引导下放射性粒子125I瘤内植入治疗腹膜后转移淋巴结的可行性、方法与近期疗效.方法 20例腹膜后淋巴结转移患者经CT引导下125I粒子瘤内植入,跟踪随访1～24个月观察近期疗效.结果 20例患者在CT引导下全部顺利完成粒子植入,未出现出血、感染等并发症.15例以腰疼症状为主的患者术后一周疼痛开始缓解,其中9例完全缓解,6例部分缓解;2例腰疼伴一侧下肢水肿患者淋巴结均有体积缩小.结论 CT引导下125I粒子植入治疗腹膜后转移淋巴结安全、微创、近期疗效满意,能有效控制局部症状.     

Study on the Influencing Factors of Cervical Lymph Node Metastasis in Papillary Thyroid Microcarcinoma

Objective:To investigate the influencing factors of the cervical lymph node metastasis in papillary thyroid microcarcinoma(PTMC) and to provide a theoretical basis for cervical lymph node dissection.Methods:A total of 80 PTMC patients treated in the Department of Breast and Thyroid Surgery of our Hospital from January 2017 to January 2018 were selected in the study.All the patients accepted the first surgical treatment and were confirmed by pathology.The clinical data of all PTMC patients were retrospectively analyzed,and the related factors such as gender,age,number of primary lesions,tumor diameter and extrathyroidal invasions were analyzed.The factors that actually affected the lymph node metastasis of PTMC patients were explored.Results:All patients underwent prophylactic central lymph node dissection.Lymph node metastasis occurred in 26 patients in the central region and in 13 patients in the lateral neck region.The rate of lymph node metastasis in the central region of men was 41.18%,and the rate of lymph node metastasis in the lateral neck region was 23.53%,which were significantly higher than that of women's 30.16% and 14.29%.The differences were statistically significant(P0.05).The regional metastasis rate of the patients of ≤45 years was 45.83%,and the lateral neck region metastasis rate was 20.83%,which were significantly higher than those of patients45 years old(12.50%,9.38%) and the differences were statistically significant(P0.05).The lymph node metastasis rate in the central region and in the lateral neck region of tumor diameter 0.5 cm were 27.27%and 9.09% respectively,which were significantly lower than those of the tumor diameter0.5-1.0 cm(38.89%,25.00%)(P0.05).The rate of lymph node metastasis in the central region of single focus was 20.37%,which was significantly lower than that of57.69% in the multifocal lesions;the rate of lymph node metastasis in the cervical region of the single lesion was 11.11%,which was significantly lower than 26.92% in the multifocal area;the differences were statistically significant(P0.05).The metastasis rate of the patients with extrathyroidal invasions in the central area was 60.00%,which is significantly higher than that without extrathyroidal invasions(30.67%).About the tumor neck invasion of the lateral neck region,the lymph node metastasis rate of the patients with extrathyroidal invasions was 40.00%,which was significantly higher than that without extrathyroidal invasions(14.67%).The differences were statistically significant(P0.05).Logistic regression analysis showed that gender and tumor size were independent influencing factors of lymph node metastasis in the central and lateral neck regions(P0.05).Conclusion:Young,male,multifocal,tumor diameter 0.5-1.0 cm and extrathyroidal invasions are risk factors for cervical lymph node metastasis in patients with PTMC.Gender and tumor size are independent factors affecting central/lateral cervical lymph node metastasis in PTMC patients.     

食管癌患者淋巴结转移灶DNA含量测定在预后判断中的意义

为了探讨食管癌患者癌周淋巴结转移(LNM)灶细胞DNA含量及其相关指标测定的预后意义,本文采用流式细胞术对64例食管癌癌周LNM灶细胞DNA含量进行检测,并对癌周LNM灶细胞的细胞生物学特性和患者预后的关系进行分析.食管癌癌周LNM灶细胞的DI值、H-检出率、SPF和PI均显著高于相应的对照组淋巴结,Apo却显著低于对照组;癌周LNM灶DNA二倍体者、倍体同质体者、SPF降低者、PI降低者、Apo升高者的术后生存期和5年生存率均显著高于DNA异倍体者、倍体异质体者、SPF升高者、PI升高者和Apo降低者.随癌周淋巴结转移阳性率的升高,患者生存期和5年生存率均逐渐降低,部分呈显著负相关关系. 结论:食管癌癌周LNM灶细胞DNA倍体变化是恶性肿瘤的重要生物学特性之一,其生物学特性和患者预后关系十分密切.因此,癌周LNM灶细胞的生物学参数可作为判断食管癌患者预后的重要参考及量化指标.