内因性葡萄膜炎患者的T淋巴细胞亚群改变

目的 旨在了解内因性葡萄膜炎患者的外周血Ｔ淋巴细胞亚群改变。方法 应用碱性磷酸酶抗碱性磷酸酶法（ＡＰＡＡＰ法）检测４５例内因性葡萄膜炎患者的外周血Ｔ淋巴细胞亚群,同期收集３２例健康对照者的外周血作为对照。结果 ４５例葡萄膜炎患者葡萄膜炎患者外周血ＣＤ３＋、ＣＤ８＋细胞数目下降,ＣＤ４＋升高,ＣＤ４＋／ＣＤ８＋比值升高。不同类型的葡萄膜炎患者的Ｔ淋巴细胞亚群数目改变不明显。经局部和／或全身皮质类固醇药物治疗后,部分患者的Ｔ淋巴细胞亚群数目有轻度改变。结论 葡萄膜炎患者体内免疫功能异常,这也为免疫治疗的观察提供了一定依据。     

人类葡萄膜炎T细胞亚群的研究

使用间接免疫灾光法测定了２０例葡萄膜炎外周血Ｔ细胞亚群。结果显示ＣＤ３＾＋细胞较对照组显著下降，ＣＤ４＾＋、ＣＤ８＾＋与ＣＤ２５＾＋细胞较对照组显著上升。ＣＤ４＾＋／ＣＤ８＾＋比值下降。提示全身免疫反应参与葡萄膜炎发病，患者可能有免疫紊乱，并有细胞免疫缺陷。     

人类复发性葡萄膜炎的T细胞亚群

材料与方法患者:10例男性,10例女性,共20例,均为复发性。包括:5例白塞氏病,5例葡萄膜大脑炎,1例福克氏综合征,1例结节病,1例交感性眼炎,3倒虹膜睫状体炎,4例其它。所有患者均未用全身激素治疗,平均年龄43.1岁。对照为20例健康献血员(18例男性2例女性),平均年龄33.95岁。材料:单克隆抗体购自武汉生物制品研究     

内源性葡萄膜炎患者细胞免疫状态的研究：以Wu系列McAb测定外周血T细胞亚群

用单克隆抗体(monoclonal antibodies,McAb)检测T细胞亚群是近年来发展起来的较为可靠的方法。本文应用Wu系列McAb以直接致敏红细胞     

复发性葡萄膜炎患者T淋巴细胞亚群检测

复发性葡萄膜炎患者Ｔ淋巴细胞亚群检测第一军医大学珠江医院眼科徐小平，徐虹，余振标慢性内源性葡萄膜炎的病因发病机理大多原因不明，临床控制复发有一定困难。该病对糖皮质激素治疗反应良好，但常因其反复发作导致较严重的并发症而预后较差。关于内源性葡萄膜炎与免疫...     

实验性自身免疫性葡萄膜炎T淋巴细胞亚群与Treg细胞的表达研究

目的　探讨实验性自身免疫性葡萄膜炎（ｅｘｐｅｒｉｍｅｎｔａｌａｕｔｏｉｍｍｕｎｅｕｖｅｉｔｉｓ,ＥＡＵ）新西兰兔模型外周血单个核细胞（ｐｅ-ｒｉｐｈｅｒａｌｂｌｏｏｄｍｏｎｏｎｕｃｌｅａｒｃｅｌｌｓ,ＰＢＭＣ）Ｔ淋巴细胞亚群的变化及其与疾病特征的相关性。方法　选取健康成年雄性新西兰大白兔４０只作为动物模型,用２０ｇ·Ｌ－１牛血清蛋白（ｂｏｖｉｎｅｓｅｒｕｍａｌｂｕｍｉｎ,ＢＳＡ）静脉注射和玻璃体内注射法建立ＥＡＵ模型,并观察模型眼炎症反应及ＰＢＭＣＴ淋巴细胞亚群特征。结果　ＣＤ４＋Ｔ细胞在ＥＡＵ兔的外周血中比例明显增加,且随时间的进展呈进行性增加,并与炎症反应呈正相关,相同的趋势可见于ＣＤ４＋／ＣＤ８＋Ｔ细胞比例。此外,Ｔｒｅｇ细胞趋势与ＣＤ４＋Ｔ细胞及ＣＤ４＋Ｔ／ＣＤ８＋Ｔ细胞比例相反,与炎症反应呈负相关。结论　ＣＤ４＋Ｔ细胞的优势性选择性克隆增殖及Ｔｒｅｇ细胞的降低有可能引发免疫功能紊乱并导致针对自身组织的免疫应答失去有效的负性调控,导致ＥＡＵ的发生及进行性加重。     

Effects of cyclosporine on T-cell subsets in experimental autoimmune uveitis

The effective inhibition of S-antigen (S-Ag) induced experimental autoimmune uveitis (EAU) by Cyclosporine (CsA) suggests strongly the important role of T-cells in the modulation of this disease. The authors evaluated the changes in T-cell subsets induced by this agent in S-Ag immunized Lewis rats. Using the fluorescence-activated cell sorter and monoclonal antibody preparations directed against rat T-cell subsets, a comparison was made between lymphocyte populations obtained from CsA or olive oil treated S-Ag immunized rats taken 5, 10, 12, and 14 days after antigenic challenge. The T-cell subpopulations of lymphocyte preparations from the spleen and peripheral blood of CsA-treated and control animals appeared to parallel each other, with both groups showing an increase in the suppressor/cytotoxic fraction beginning on day 12 and approaching the percentage of inducer cells by day 14. Lymphocyte preparations from lymph nodes draining the site of S-Ag immunization from CsA-treated animals demonstrated a different T-cell subset profile than did controls. Beginning on day 10, the control group was noted to have an increased inducer cell fraction as compared with the CsA group. This increase in the inducer fraction paralleled an increase in the in vitro proliferative responses to the S-Ag. These data suggest that CsA appears to prevent the development of inducer cells in the lymph nodes draining the S-Ag immunization site, the T-cell subgroup the authors have seen capable of inducing EAU.     

Lymphocyte subsets of peripheral blood in patients with sarcoidosis]

Cytofluorometric analysis of lymphocyte subsets was performed in the peripheral blood samples from fifteen sarcoidosis patients with uveitis as well as normal controls. The percentage of T cells (CD3+) was significantly decreased, whereas that of B cells (CD19+) was markedly increased in patients with sarcoidosis compared to controls. Natural killer cells (CD8+CD57+) as well as activated T cells (CD3+HLA-DR+) were significantly increased in patients with sarcoidosis. The percentage of the Leu 1-B cells and the ratio of Leu 1-B/B were significantly higher in sarcoidosis patients with respect to controls. Moreover, the ratio of Leu 1-B/B was significantly correlated with the serum level of angiotensin converting enzyme (ACE) in sarcoidosis. Therefore, it is considered that the Leu 1-B/B value could be a useful indicator not only for the activity but also for the diagnosis of sarcoidosis. No significant difference was found in any subset between the patients with active uveitis and patients with inactive uveitis.     

Hemosorption in multimodal treatment of endogenous uveitis]

Hemoperfusion was used in multiple modality treatment of 92 patients with chronic recurrent endogenic uveitis and uveitis associated with syndromic conditions. The treatment included systemic and local steroids, and antiinflammatory therapy. Clinical improvement was achieved in 75 percent of patients; it was paralleled by improvement of the vision acuity. In the rest patients vision acuity was unchanged because of irreversible impairment of the retina and optic nerve, but uveitis ceased. Addition of hemoperfusion of multiple-modality treatment of uveitis patients resulted in shortening of hospital stay by 6-7 days, in prolongation of remission periods to 10-15 months after a single course of hemoperfusion in 71 percent of patients and after two courses in the rest. A dynamic immunologic analysis has shown normalization of T lymphocyte functional activity, and of blood serum immunoglobulin and circulating immune complexes levels.     

The importance of immunologic tests in endogenous uveitis]

The authors evaluate the results of immunological examination of the peripheral blood of 50 patients with endogenous uveitis hospitalized at the Ophthalmological Clinic in Plzen in 1987-1989. The authors reach the conclusion that with regard to contemporary, available methods we cannot expect a significant contribution of immunological examinations of the peripheral blood for the diagnosis of endogenous uveitis without systemic disease.