商陆多糖I对小鼠脾淋巴细胞增殖及脾淋巴细胞、巨噬细胞分泌细胞因子的影响

商陆多糖Ⅰ(Phytolacca acinosa polysaccharides Ⅰ,PAP-Ⅰ)体外能显著促进小鼠脾淋巴细胞增殖,促进丝裂原诱导的淋巴细胞转化及双向混合淋巴细胞反应。检测PAP-Ⅰ和小鼠脾淋巴细胞培养上清液发现PAP-Ⅰ可显著促进小鼠脾淋巴细胞产生白细胞介素2(IL-2)及集落刺激因子(colony stimulating factor,CSF)。PAP-Ⅰ和小鼠腹腔巨噬细胞培养上清液中也存在CSF活性及促进重组粒单系集落刺激因子诱导骨髓细胞增殖的细胞因子。PAP-Ⅰ,ip可显著促进小鼠脾淋巴细胞增殖及IL-2产生。     

商陆多糖Ⅱ体外对小鼠脾细胞增殖及产生集落刺激因子的影响

用[³H]TdR参入法检测小鼠脾细胞增殖能力及产生集落刺激因子(colony stimulating factor. CSF)含量.证明商陆多糖Ⅱ(PAP-Ⅱ)在31～500 μg·m^-1范围内显著促进小鼠稗细胞增殖。PAP-Ⅱ,31～125 μg·ml^-1可剂量依赖性地促进Con A(1,2.8μg·ml^-1),LPS(3,10,30 μg·ml^-1)诱导的淋巴细胞细咆增殖,随着PAP-Ⅱ剂量加大,对丝裂原诱导的淋巴细胞增殖反呈抑制作用。PAP-Ⅱ。10～500μg·ml^-1呈剂量及时间依赖性地促进脾细胞产生CSF,其最适剂量为100 μg·ml^-1。最佳时间为5 d,提示PAP-Ⅱ能增强免疫及促进造血功能。     

牛膝多糖衍生物对小鼠脾淋巴细胞增殖及诱生IL-2和TNF-α的影响

目的研究牛膝多糖硫酸酯(S-AbP)和磷酸酯(P-AbP)衍生物对小鼠脾淋巴细胞增殖及诱生IL-2和TNF-α的影响。方法四甲基偶氮唑盐(MTT)法检测淋巴细胞增殖,ELISA法测定培养上清液中IL-2和TNF-α的含量。结果S-AbP和P-AbP均能剂量依赖性地促进小鼠脾淋巴细胞增殖,S-AbP和P-AbP的促增殖活性较AbP强。P-AbP显著诱导脾淋巴细胞分泌TNF-α,对IL-2的分泌无明显影响。S-AbP能诱生较高含量的IL-2,而对TNF-α的分泌无明显影响。结论S-AbP和P-AbP较牛膝多糖能更有效地促进淋巴细胞增殖,还可以分别通过IL-2和TNF-α发挥免疫调节作用。     

海洋真菌多糖(YCP)对小鼠淋巴细胞免疫功能的影响

目的研究海洋真菌多糖(YCP)促进小鼠淋巴细胞的免疫调节作用,探讨其抗肿瘤作用机制。方法采用四甲基偶氮唑盐(MTT)法检测YCP对淋巴细胞增殖的作用,用酶联免疫吸附试验(ELISA)法检测白介素-2(IL-2)和干扰素-γ(IFN-γ)的含量,MTT法检测淋巴因子激活的杀伤细胞(LAK细胞)的诱生及其细胞杀伤活性。结果YCP体外能显著提高淋巴细胞增殖反应;显著增加细胞上清液中IL-2和IFN-γ的浓度;与IL-2单独刺激相比,YCP体外能显著提高与IL-2协同诱导产生的LAK细胞对于NK不敏感的Hela细胞和NK敏感的K562细胞的杀伤作用。结论YCP能够增强小鼠脾淋巴细胞的免疫作用。     

MTT法检测卵蛋白刺激哮喘小鼠脾淋巴细胞增殖最佳实验条件的研究

目的探讨MTT法检测卵蛋白(OVA)刺激哮喘小鼠脾淋巴细胞增殖的最佳实验条件。方法制备OVA诱导哮喘小鼠模型,分离脾淋巴细胞。接种不同浓度的脾淋巴细胞,在不同培养时间点测定吸光度(A)值,确定最佳细胞浓度和检测时间。观察不同浓度OVA对脾淋巴细胞增殖的影响。结果最佳脾淋巴细胞接种浓度为2×106~4×106cells/ml,最佳检测时间为48 h。培养24和48 h时,细胞浓度为1×106~4×106cells/ml,A值与细胞浓度呈线性关系(r0.99)。10μg/ml OVA可诱导脾淋巴细胞显著增殖,100μg/ml OVA反而抑制脾淋巴细胞增殖。结论 MTT法可用于检测哮喘小鼠的脾淋巴细胞增殖,细胞浓度与A值呈线性关系。OVA浓度是影响脾淋巴细胞增殖的重要因素。     

雷帕霉素对小鼠脾淋巴细胞走后门殖及产生细胞因子的影响

目的：研究雷帕霉素（RAPA）对ConA、PHA、LPS诱导的小鼠脾细胞增殖反应,混合淋巴细胞反应,脾T淋巴细胞亚型及细胞因子IL-2、IFN-γ、TNF-α含量的影响。方法：MTT法测定淋巴细胞增殖实验、混合淋巴细胞反应,流式细胞法测定脾T淋巴细胞亚型,结晶紫染色法测定TNF-α,E-LASI法测定IL-2,IFN-γ。结果：RAPA可显著抑制ConA、PHA和LPS诱导的小鼠脾细胞增殖和混合淋巴细胞反应（MLR）,对小鼠脾T淋巴细胞亚型无明显影响。RAPA还可明显抑制小鼠脾淋巴细胞分泌IL-2,IFN-γ,但对小鼠腹腔巨噬细胞分泌TNF-α无效。结论：RAPA抑制小鼠免疫功能的作用机制与CsA不同。     

大鼠松果腺培养上清对淋巴细胞功能的影响

本文观察了异丙肾上腺素(10μmol/L)与松果腺体外培养60min上清对淋巴细胞功能的影响。结果表明,松果腺培养上清1/45稀释度时可分别促进ConA和LPS诱导小鼠脾淋巴细胞增殖反应,1 /135稀释度可促进ConA诱导大鼠脾细胞IL-2的产生。     

褪黑素对淋巴细胞功能的影响

本文采用~3H-TdR掺入法和ConA活化脾细胞检测IL-2的方法观察了松果腺褪黑素(MT)对ConA和LPS诱导小鼠脾T_1,B淋巴细胞增殖反应及ConA诱导大鼠脾淋巴细胞产生IL-2的影响。结果表明,MT0.01～1μmol/L和0.01～10μmol/L分别促进ConA和LPS诱导小鼠脾淋巴细胞增殖反应,ATl～10μmol/L对ConA诱导大鼠脾淋巴细胞产生IL-2亦有促进作用,但MT对未经ConA或LPS活化的淋巴细胞功能则无明显影响,提示丝裂原活化的淋巴细胞可能是MT作用的靶细胞之     

儿茶素对环磷酰胺诱导小鼠迟发性超敏反应的影响*

目的:研究儿茶素对环磷酰胺诱导小鼠迟发性超敏反应的影响。方法:以2,4-二硝基氯苯(DNCB)诱导小鼠迟发性超敏反应(DTH),致敏后立即腹腔注射(ip)环磷酰胺(Cy)125 mg.kg-1和致敏前3 d ip Cy 250mg.kg-1分别诱导小鼠降低和增强的DTH反应,用耳肿胀度、胸腺指数和脾指数作为主要检测指标。以ConA诱导胸腺T淋巴细胞,观察不同剂量或浓度的儿茶素体内外对胸腺T淋巴细胞增殖和IL-2生成的影响。MTT法检测胸腺T淋巴细胞增殖,ConA诱导的胸腺T淋巴细胞培养上清中IL-2活性检测采用活化的小鼠胸腺细胞MTT比色法。结果:于致敏前3 d灌胃儿茶素30,60,120 mg.kg-1.d-1,连续7 d,在Cy诱导的DTH反应降低小鼠模型,儿茶素在60,120 mg.kg-1剂量可显著上调其低下的耳肿胀度、胸腺和脾指数,并上调胸腺T淋巴细胞增殖反应以及产生的IL-2水平;在Cy诱导的DTH反应增强小鼠模型,儿茶素60,120 mg.kg-1可显著下调其增高的耳肿胀、胸腺和脾指数,儿茶素30,60,120 mg.kg-1可显著抑制其增强的胸腺T淋巴细胞增殖反应以及产生的IL-2水平。儿茶素体外在25~200 mg.L-1浓度时能增强Cy诱导的DTH反应降低的小鼠胸腺T淋巴细胞增殖和IL-2水平;儿茶素在12.5~200 mg.L-1浓度时能降低Cy诱导的DTH反应增强的小鼠胸腺T淋巴细胞增殖和IL-2水平。结论:儿茶素对小鼠的细胞免疫具有调节作用。     

甜菜碱对S180荷瘤小鼠脾淋巴细胞增殖的影响

目的:以S180荷瘤小鼠脾淋巴细胞作为研究材料,研究甜菜碱对S180町荷瘤小鼠脾淋巴细胞增殖影响的内在机制.方法:MTF法测S180荷瘤小鼠脾淋巴细胞增殖程度.ELISA方法检测甜菜碱对S180荷瘤小鼠脾淋巴细胞分泌肿瘤坏死因子α的含量的影响.结果:培养48 h后,甜菜碱在50-5 000μg/ml,的浓度范围内可促进S180荷瘤小鼠脾淋巴细胞的增殖反应(P<0.01).甜菜碱在浓度为50～500μg/ml的范围内可促进S180舶荷瘤小鼠脾淋巴细胞TNF-α的产生(P<0.01).结论:甜菜碱能够促进S180荷瘤小鼠脾淋巴细胞增殖、促进TN-α的产生,说明甜菜碱的促进增殖作用主要与促进TNF-α的产生有关.     

PHASE TWO OF AN INTERLABORATORY EVALUATION OF THE QUANTIFICATION OF RAT SPLENIC LYMPHOCYTE SUBTYPES USING IMMUNOFLUORESCENT STAINING AND FLOW CYTOMETRY

In phase one of an interlaboratory study, baseline values for rat splenic lymphocyte populations were established. In phase two, rat splenic lymphocyte populations were evaluated using immunofluorescent staining and flow cytometry following exposure to the immunosuppressive agent cyclophosphamide (CY). The study involved four independent facilities employing a common protocol. All laboratories purchased animals and reagents from the same sources. The objective of phase two was to determine whether each laboratory could detect a significant change in the same splenic lymphocyte population(s) at the same or similar CY dose levels. Crl:CD R BR male rats were dosed by the intraperitoneal route with 1, 3, or 10 mg / kg CY for 4 days. On day 5, spleen cell number and weights were obtained and splenic lymphocytes were evaluated following the lysis of red blood cells with ammonium chloride. Splenic lymphocyte populations were enumerated with monoclonal antibodies using the dual labeling of T-cell subpopulations and quadrant analysis procedures. The no observable adverse effect level (NOAEL) for spleen weight was 1 mg / kg for two laboratories and 3 mg / kg for the remaining two laboratories. For spleen cell number, the NOAEL was 1 mg / kg for three of the laboratories and between 3 and 10 mg / kg for the fourth. For the relative percentages of each splenic lymphocyte population, three of the four laboratories were within one dose level of each other for the NOAEL. The NOAEL for the W3/25+OX19+/OX8+OX19+ ratio was 3 mg/kg for three of the laboratories and < 1 mg / kg for the remaining laboratory. With the exception of the absolute numbers of OX8+OX19+ cells, the NOAELs for each absolute lymphocyte population were within one dose level for three of the laboratories. The fourth laboratory had NOAELs for each absolute population of > 10 mg/kg. Using flow cytometry, each of the laboratories identified CY as an immunotoxic agent.     

灵芝多糖对混合淋巴细胞培养反应中白细胞介素2生成和T细胞亚类的影响(英文)

利用混合淋巴细胞培养反应研究了灵芝多糖的免疫作用机理。结果表明培养12h,灵芝多糖(25,50,100,200μg/ml)可促进白细胞介素2的分泌,且具有剂量依赖关系。培养4d后,可增加总的细胞回收量以及Lyt 2~+和L3T4~+细胞的回收量。灵芝多糖还明显增强细胞毒T细胞的功能,在浓度为200μg/ml时,其杀伤活性增加100％。     

灵芝多糖对混合淋巴细胞培养反应中白细胞介素2生成和T细胞亚类的影响(英文)

利用混合淋巴细胞培养反应研究了灵芝多糖的免疫作用机理。结果表明培养12h,灵芝多糖(25,50,100,200μg/ml)可促进白细胞介素2的分泌,且具有剂量依赖关系。培养4d后,可增加总的细胞回收量以及Lyt 2⁺和L3T4⁺细胞的回收量。灵芝多糖还明显增强细胞毒T细胞的功能,在浓度为200μg/ml时,其杀伤活性增加100%。     

IRON EXACERBATES ANILINE-ASSOCIATED SPLENIC TOXICITY

Our earlier studies have shown that aniline exposure in rats causes time- and dose-dependent accumulation of iron in the spleen, which may exacerbate aniline splenotoxicity by catalyzing free-radical reactions. The present studies were conducted to test whether aniline-induced splenic toxicity could be potentiated by iron overload. For 30 d male Sprague-Dawley rats received the following treatments: 0.5 mmol/kg/ d aniline hydrochloride (AH) by gavage (AH group); 3% carbonyl iron-supplemented diet (IR group); 0.5 mmol/kg/d AH by gavage and iron-supplemented diet (AH + IR group); or no treatments (controls). Treatment-related significant increases in total iron, low molecular weight chelatable iron, lipid peroxidation, and protein oxidation were observed in the spleens of all the groups compared to control. However, these changes were much greater in the combined AH + IR group. The aniline-induced morphological changes in the spleen were consistent with our earlier observations, but were more pronounced in the AH + IR group. The increased toxicity, as evident from greater oxidative stress and morphological changes in the AH + IR group, suggests that iron potentiates the splenic toxicity of aniline.     

15-甲基前列腺素F_(2α)对纯种Beagle狗周围血淋巴细胞移动及增殖的影响

本文观察纯种性成熟Beagle狗im15-甲基前列脉素F_(2α)0.5mg/kg qd×14d,对周围血淋巴细胞的移动能力及淋巴细胞增殖的影响,结果对两者均无显著影响     

SERUM IMMUNOGLOBULINS AND LYMPHOCYTE SUBSET DISTRIBUTIONS IN CHILDREN AND ADULTS LIVING IN COMMUNITIES ASSESSED FOR LEAD AND CADMIUM EXPOSURE

A short-term evaluation of 6 months of estrogen therapy on oxidant status in 38 postmenopausal women was conducted. The levels of serum lipid peroxidation products, glutathione (GSH) status, and glutathione-related enzymes were evaluated before and after 6 months of hormone replacement therapy. After 6 months of estrogen treatment there was a significantly increased concentration of thiobarbituric acid-reactive substances (TBARS), which are an end product of lipid peroxidation. This was accompanied by a significant increase in the activity of glutathione peroxidase (GSH-Px). However, the activities of glutathione reductase (GSSG-R) and superoxide dism utase (SOD) were significantly decreased and total protein thiols were reduced. Data suggest that hormone replacement therapy in postmenopausal women is associated with oxidant mechanisms.     

当归多糖对小鼠脾淋巴细胞增殖及诱生IFN-γ的影响

目的研究当归多糖对小鼠脾淋巴细胞增殖及诱生IFN-γ的影响。方法用4种不同浓度乙醇将当归总多糖(AP)进行分级沉淀,获得AP-I,AP-II,AP-III和AP-IV 4个部分多糖。用［³H］-TdR参入法检测当归部分多糖对小鼠脾淋巴细胞增殖作用;结晶紫染色法测定IFN-γ的生物活性;ELISA法测定IFN-γ的含量。结果AP-I和AP-II能明显促进体内外小鼠脾淋巴细胞增殖,体外显著诱导脾淋巴细胞分泌IFN-γ,增强IFN-γ的生物活性;特别是AP-II体外诱生较高含量的IFN-γ,AP-I体内诱生的IFN-γ生物活性较强。结论AP-I和AP-II是免疫活性较强的部分。