共查询到20条相似文献,搜索用时 31 毫秒
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Two catabolite activator protein molecules bind to the galactose promoter region of Escherichia coli in the presence of RNA polymerase 总被引:13,自引:2,他引:13 下载免费PDF全文
Stephanie H. Shanblatt Arnold Revzin 《Proceedings of the National Academy of Sciences of the United States of America》1983,80(6):1594-1598
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Single amino acid substitutions in the cAMP receptor protein specifically abolish regulation by the CytR repressor in Escherichia coli. 下载免费PDF全文
L S?gaard-Andersen A S Mironov H Pedersen V V Sukhodelets P Valentin-Hansen 《Proceedings of the National Academy of Sciences of the United States of America》1991,88(11):4921-4925
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J D Gralla 《Proceedings of the National Academy of Sciences of the United States of America》1985,82(10):3078-3081
A DNase protection technique is described and applied to the interaction of three lac control proteins with supercoiled lac DNA. The technique uses end-labeled oligonucleotide primers to probe specific DNA regions as an alternative to protocols requiring restriction endonuclease cleavage or blotting. Thus DNA may be probed with high resolution in its native state. It is demonstrated that the introduction of supercoiling into DNA accelerates the rate of lac ps promoter binding by RNA polymerase but does not alter the positions at which polymerase, c-AMP-binding protein, or lac repressor bind to lac DNA. 相似文献
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Application of fluorescence energy transfer and polarization to monitor Escherichia coli cAMP receptor protein and lac promoter interaction. 总被引:9,自引:0,他引:9 下载免费PDF全文
T Heyduk J C Lee 《Proceedings of the National Academy of Sciences of the United States of America》1990,87(5):1744-1748
A fluorescence method was developed to study DNA-protein interactions in solution. A 32-base-pair (bp) DNA fragment of the lac promoter containing the primary binding site for Escherichia coli cAMP receptor protein (CRP) was chemically synthesized and labeled specifically at the 5' end with fluorescent probe. Binding of cAMP receptor protein to this fragment can be conveniently followed by measuring changes in polarization of fluorescence of the labeled DNA or by measuring fluorescence energy transfer from protein tryptophan residues to the DNA label. Formation of protein-DNA complex was monitored as a function of cAMP concentration. Various equilibrium constants can be resolved to characterize the binding of cAMP to CRP and the subsequent binding of CRP-cAMP and CRP-(cAMP)2 to DNA. These binding studies showed that the two ligated forms of CRP have significantly different affinities for specific-site DNA. These results show that, in principle, the fluorescence technique can yield thermodynamically valid equilibrium constants under essentially any solution conditions. This technique also has the potential of providing information regarding the structure of protein-DNA complexes. 相似文献
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Purification and DNA-Binding Properties of the Catabolite Gene Activator Protein 总被引:26,自引:4,他引:22 下载免费PDF全文
A. D. Riggs G. Reiness G. Zubay 《Proceedings of the National Academy of Sciences of the United States of America》1971,68(6):1222-1225
A protein required for the activation of the lac operon has been extensively purified and partly characterized. This protein, called CGA protein (catabolite gene activator protein, sometimes named CAP), is a dimer with subunits of 22,000 daltons. Purified CGA protein has a substantial affinity for DNA; this affinity is greatly strengthened by cAMP and strongly inhibited by cGMP. Other studies have shown that these cyclic nucleotides compete for a binding site on CGA protein. The opposing effects of the two cyclic compounds in DNA-CGA protein binding show a parallel behavior to their effects on the expression of the lac operon. Thus cAMP, in addition to CGA protein, is required for expression of the lac operon, whereas cGMP inhibits the expression. The obvious inference is that CGA protein activates the lac operon by binding to the DNA under the influence of cAMP. Thus, CGA protein seems to be a new type of regulatory protein: a DNA-binding activator. 相似文献
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P G Debenham O Pongs A A Travers 《Proceedings of the National Academy of Sciences of the United States of America》1980,77(2):870-874
Escherichia coli fMet-tRNAfMet alters the pattern of promoter selection of E. coli RNA polymerase (RNA nucleotidyltransferase, nucleosidetriphosphate:RNA nucleotidyltransferase; EC 2.7.7.6), affecting RNA synthesis from the rRNA, suIII+tRNA, and lac promoters in different ways. The in vitro synthesis of the stable RNA species is selectively decreased, whereas that of lac RNA from both the wild-type and mutant UV5 promoters is selectively increased at high ionic strength. The functional effect of fMet-tRNAfMet resembles that of the nucleotide guanosine 3'-diphosphate 5'-diphosphate (ppGpp). This nucleotide competes with the binding of fMet-tRnafMet to RNA polymerase. 相似文献