半夏泻心汤调节胃肠动力的物质基础研究

目的:探讨半夏泻心汤调节胃肠动力的物质基础。方法:正常大鼠、阿托品致胃肠动力障碍大鼠、慢性水浸束缚致胃肠动力障碍大鼠分别ig给予蒸馏水、多潘立酮(0.01 g·kg-1)、半夏泻心汤(2 g·kg-1)、辛开药液(1.5 g·kg-1)、苦降药液(1.5 g·kg-1)、甘补药液(1.5 g·kg-1),连续4 d,通过测定大鼠胃残留率、小肠推进率筛选半夏泻心汤调节胃肠动力的有效药物组分;灌胃蒸馏水、多潘立酮(0.01 g·kg-1)、半夏泻心汤醇沉药液(2 g·kg-1)、半夏泻心汤醇沉水溶药液(2 g·kg-1)、苦降醇沉药液(1.5 g·kg-1)、苦降醇沉水溶药液(1.5 g·kg-1),同法筛选其有效部位。结果:与正常大鼠比较,两种模型大鼠,胃残留率明显上升,小肠推进率明显下降,且均有统计学意义(P0.05,P0.01);在给予不同药液后,两项指标均有不同程度的变化,其中半夏泻心汤能降低阿托品模型大鼠胃残留率,半夏泻心汤醇沉药液、苦降药液和苦降醇沉药液能降低正常大鼠和两种模型大鼠的胃残留率,以上变化均具有统计学意义(P0.05,P0.01)。结论:半夏泻心汤有调节胃肠动力的作用,其有效药物组分主要为苦降药组,其有效部位为苦降药的醇沉部位。     

加味黄芪建中汤对大鼠乙酸胃溃疡胃黏膜的保护作用

目的探讨加味黄芪建中汤对胃黏膜的保护作用机制。方法将60只W istar大鼠随机分成,正常对照组、模型组、加味黄芪建中汤大剂量组(20 g/kg)、加味黄芪建中汤中剂量组(10 g/kg)、加味黄芪建中汤小剂量组(5 g/kg)和果胶铋组各10只,采用100%乙酸诱导的慢性胃溃疡模型,给予相应治疗16 d后,观察胃黏膜的病理、血液中前列腺素(6-K-PGF1α)、胃液中表皮生长因子(EGF)、胃组织中一氧化氮(NO)及血液中超氧化歧化酶(SOD)变化。结果加味黄芪建中汤促进了胃溃疡黏膜修复、显著提高了血浆6-k-PGF1α、胃液中EGF、胃组织中NO及血浆SOD水平。结论加味黄芪建中汤对大鼠乙酸胃溃疡胃黏膜有明显的保护作用。     

藏药十五味黑药丸对大鼠胃肠运动的影响

目的:研究十五味黑药丸对大鼠胃肠运动的影响。方法:以胃肠道标记物葡聚糖蓝2000的胃内相对残留率和小肠推进率为指标,观察十五味黑药丸对大鼠胃排空及小肠推进运动的影响;通过生物信号采集与处理系统记录大鼠胃纵形肌条张力参数,观察该药对胃条张力的影响。结果:十五味黑药丸能明显降低葡聚糖蓝2000的胃内相对残留率及增加其小肠推进率,十五味黑药丸药液能明显增加胃条张力。结论:十五味黑药丸能促进胃肠运动。     

干地黄煎剂防止无水乙醇性胃粘膜损伤作用及其机制

目的:研究干地黄对胃粘膜的保护作用及机制。方法:给大鼠胃饲无水乙醇1ml 造模,观察干地黄对胃粘膜损伤的影响;采用不同途径给药和预先肌肉注射消炎痛进行分析。结果:(1)分别提前15、30和120min 胃饲干地黄煎剂6g/kg 均能显著抑制胃粘膜损伤;(2)分别胃饲3、6和12g/kg 的干地黄煎剂能不同程度地抑制胃粘膜损伤,且有一定的量效对应关系;(3)十二指肠内注射干地黄煎剂6g/kg 对胃粘膜亦有保护作用;(4)预先注射消炎痛5mg/kg 不能取消干地黄对胃粘膜损伤的预防作用。结论:干地黄对胃粘膜有明显的保护作用,其对无水乙醇性损伤的预防机理可能与胃腔内的理化作用及前列腺素合成无关。     

Protective effects of neohesperidin and poncirin isolated from the fruits of Poncirus trifoliata on potential gastric disease

The effects of Poncirus trifoliata (P. trifoliata) (Ponciri Fructus, PF) extract and its constituents such as neohesperidin and poncirin on gastritis in rats and human gastric cancer cells were investigated. The PF 70% ethanol extracts (1 g) showed approximately 11.38% of acid‐neutralizing capacities and cytotoxicity (IC₅₀ = 85.39 µg/mL) against human AGS gastric cancer cells. In addition, neohesperidin exhibited antioxidant activity (IC₅₀ = 22.31 µg/mL) in the 1,1‐diphenyl‐2‐picryldydrazyl (DPPH) radical‐scavenging assay. Neohesperidin (50 mg/kg) and poncirin (100 mg/kg) significantly inhibited 55.0% and 60.0% of HCl/ethanol‐induced gastric lesions, respectively, and increased the mucus content. In pylorus ligated rats, neohesperidin (50 mg/kg) significantly decreased the volume of gastric secretion and gastric acid output, and increased the pH. From these results, it could be suggested that neohesperidin and poncirin isolated from PF may be useful for the treatment and/or protection of gastritis. Copyright © 2009 John Wiley & Sons, Ltd.     

三仁汤对脾胃湿热型大鼠NLRP3、Caspase-1蛋白的影响＊

目的 探究三仁汤对脾胃湿热型大鼠NLRP3、Caspase-1蛋白的调控及胃黏膜细胞焦亡的影响。方法 将50只雄性SD大鼠随机分为正常组、模型组、三仁汤高、中、低剂量组，每组各10只。根据前期的研究方法，利用高脂、高糖、湿热的条件，复制脾胃湿热证的模型，给予三仁汤干预，高、中、低剂量分别为16.4 g/kg，8.2 g/kg，4.1 g/kg。HE染色观察胃黏膜病理变化，免疫印迹法检测大鼠胃黏膜细胞NLRP3、Caspase-1蛋白的表达。结果HE结果显示，三仁汤高、中、低剂量组均对胃黏膜细胞焦亡有所改善。免疫印迹的结果显示模型组大鼠NLRP3和Caspase-1高表达，三仁汤高、中、低剂量组下调NLRP3和Caspase-1蛋白的表达，具有统计学差异（P < 0.05）。结论 三仁汤可能通过降低脾胃湿热大鼠的NLRP3、Caspase-1蛋白的表达，减少胃粘膜细胞焦亡，抑制胃粘膜炎症的爆发，从而防治脾胃湿热证模型大鼠，为临床治疗脾胃湿热证的胃痛提供了理论依据。     

Effect and mechanism of ethanol extracts of muxiang(Radix Aucklandiae) on gastric ulcers in rats

OBJECTIVE: To investigate the effect of ethanol extracts of Muxiang(Radix Aucklandiae)(RA) on gastric ulcers in rats and explore the potential mechanisms.METHODS: A model was established by ethanol(0.75 m L/kg). According to body weight, rats were pretreated with RA extracts(2.5 or 5 g/kg). The rats were administered 95% ethanol orally after 1 h. The effects of ethanol were evaluated by measuring the gastric secretion volume, p H, pepsin activity, and ulcer area. Histological analysis and immunohistochemistry were also conducted. Furthermore, the effect of the ethanol extract of RA on transiting activity of the gastrointestinal tract was observed in mice.RESULTS: Intragastric administration of RA extracts protected the gastric mucosa from ethanol-induced gastric ulcers, while reducing submucosal edema and preventing hemorrhagic damage.Moreover, the extracts increased the production of gastric mucus, upregulated Bcl-2, and downregulated Bax expression. Importantly, pretreated rats exhibited no significant change in the gastric secretion volume, gastric juice acidity, or pepsin. Furthermore, pretreatment prominently(P 0.05) enhanced propulsive movement of the gastrointestinal tract in normal mice and mice with gastrointestinal motility disorders.CONCLUSION: Ethanol extracts of RA ameliorated gastric lesions in the gastric ulcer rat model. The mechanisms of action were related to improvement of gastrointestinal dynamics, maintenance of mucus integrity, and inhibition of apoptosis by downregulating proapoptotic Bax protein and upregulating anti-apoptotic Bcl-2 protein.     

Effect of Commiphora opobalsamum (L.) Engl. (Balessan) on experimental gastric ulcers and secretion in rats

The ulcer protective potential of an ethanol extract of Commiphora opobalsamum (L.) Engl. (Burseraceae) 'Balessan' was assessed against different acute gastric ulcer models in rats induced by necrotizing agents (80% ethanol, 0.2M NaOH and 25% NaCl), hypothermic restraint stress, pyloric ligation (Shay) and indomethacin. Balessan, 250 and 500 mg/kg administered orally (intraperitoneally in Shay rat model) showed a dose-dependent ulcer protective effects in all the above ulcer models. Besides, the extract offered protection against ethanol-induced depletion of stomach wall mucus and reduction in nonprotein sulfhydryl (NP-SH) concentration. Ethanol treatment also caused histopathological lesions of the stomach wall. Pretreatment with Balessan extract provided a complete protection of gastric mucosa through supporting both the offensive and defensive factors. Balessan extract was also showed a large margin of safety without any apparent adverse effects in rats.     

Protective effect of ethyl acetate fraction of Acacia ferruginea DC. against ethanol-induced gastric ulcer in rats

Ethnopharmacological relevance

In traditional systems of medicine, stem bark of Acacia ferruginea DC. is used for the treatment of itching, leucoderma, ulcers, stomatitis and diseases of the blood. In the present study, we determined antioxidant and anti-ulcerogenic activities of Acacia ferruginea stem bark.

Materials and methods

Acetone extract and its sub-fractions of Acacia ferruginea stem bark were subjected to assess their antioxidant potential using various in vitro systems such as DPPH^•, ABTS^•+ scavenging, FRAP and phosphomolybdenum reduction activities. Based on the antioxidant potential, the ethyl acetate fraction was used to evaluate the protective effect of ethanol-induced gastric damage in rat model. Enzyme activities such as superoxide dismutase, glutathione, catalase and lipid peroxidation were also determined in the stomach tissues.

Results

Ethyl acetate fraction (AFE) of Acacia ferruginea stem bark registered higher antioxidant and free radical scavenging activities than the crude acetone extract and other fractions. In addition, AFE exhibited that the IC₅₀ values of DPPH (2.5 µg/ml) and ABTS (1.8 µg/ml) were lower when compared to the standard quercetin (12.4 µg/ml and 4.7 µg/ml, respectively). In ethanol induced gastric ulcer, administration of AFE at doses of 10 mg/kg, 50 mg/kg and 100 mg/kg body weight prior to ethanol ingestion significantly protected the stomach ulceration. Consequently significant changes were observed in enzyme activities such as SOD, CAT, GSH and LPO in the stomach tissues when compared with ethanol control group.

Conclusion

It is concluded that the ethyl acetate fraction of Acacia ferruginea stem bark possessed higher antioxidant and anti-ulcerogenic activities. Based on the results, we suggest that Acacia ferruginea stem bark has potential to provide a therapeutic approach to ethanol mediated ulcer as an effective anti-ulcer agent.     

小柴胡汤中柴胡不同剂量对正常大鼠胃窦组织MTL,AChE,NO含量的影响

目的:观察小柴胡汤中柴胡不同剂量对正常大鼠胃窦组织胃动素(MTL)、乙酰胆碱酯酶(AChE)、一氧化氮(NO)的影响,并探讨其作用机制.方法:40只大鼠随机分为4组:正常对照组、小柴胡汤组[柴胡高剂量组(高)、柴胡中剂量组(中)、柴胡低剂量组(低)].每天ig给予相应的小柴胡汤水煎液药液浓缩剂(高、中、低剂量分别为13,10,9.1 g·kg-1)或等容积生理盐水ig,连续3d.末次给药后,ELISA法测定胃窦组织MTL,AChE,NO的含量.结果:与正常对照组比较,柴胡剂量最高的小柴胡汤组(高)胃窦组织MTL,AChE的含量升高(P＜0.05),而胃窦组织NO的含量则无统计学差异;小柴胡汤组(中)、(低)的胃窦组织MTL,AChE,NO与正常组比均无统计学差异.结论:应用高剂量柴胡的小柴胡汤促进胃动力的作用机制,可能与增加胃窦组织MTL,AChE的含量有关.     

舒胃汤对功能性消化不良大鼠胃肠动力及ENS-ICC-SMC网络超微结构的影响

目的: 观察舒胃汤对功能性消化不良(functional dyspepsia,FD) 肝郁脾虚型大鼠胃肠动力及胃肠道神经-Cajal间质细胞-平滑肌(ENS-ICC-SMC)网络超微结构的影响,探讨舒胃汤治疗 FD 的机制. 方法: 将72只大鼠随机分为正常组、模型组、舒胃汤低剂量组(舒低组)、舒胃汤中剂量组(舒中组) 和舒胃汤高剂量组(舒高组),莫沙比利组(西药组),每组 12只.舒低、中、高分别给予舒胃汤 7.67,15.34,30.68 g·kg^-1,莫沙必利组予莫沙必利 1.37 mg·kg^-1.采用复合病因造模(慢性束缚应激+过度疲劳+饮食失节),造成FD肝郁脾虚证大鼠模型.造模后第3天各组给予相应药液,对照组和模型组每日予以蒸馏水(10 mL·kg^-1),均为每日1次,持续14 d.检测大鼠胃内残留率,小肠推进率,电镜下观察大鼠胃窦部和十二指肠组织ENS-ICC-SMC网络超微结构. 结果: 与对照组比较,模型组胃排空延迟,胃内残留率升高,小肠推进率下降(P<0.05).与模型组比较,舒高组、舒中组、莫沙必利组胃内残留率明显降低,小肠推进率明显升高(P<0.01).与模型组比较,舒高组、舒中组、莫沙必利组ICC细胞形态改善,ICC周围神经末梢较丰富,ICC相互之间及ICC与平滑肌细胞之间联系紧密. 结论: 舒胃汤能够改善ENS-ICC-SMC网络超微结构之间的相互联系,改善胃肠动力,从而有效治疗FD.     

Effects of TDEYA from Atractylodes rhizome on experimental gastric ulcer

The administration of (6E, 12E)-tetradecadiene-8, 10-diyne-1, 3-diol diacetate (TDEYA), isolated from Atractylodes rhizome, promoted the transit of charcoal in the small intestine of mice at a dose of 250 mg/kg. TDEYA prevented lesion formation induced by HCl-ethanol or ethanol in a dose-dependent manner. This action was still evident following intraperitoneal administration and previous treatment with indomethacin. The protective effect of TDEYA against gastric lesion caused by necrotizing agents would thus appear not to be mediated by endogenous prostaglandins. TDEYA did not inhibit gastric ulcers induced by pyloric ligation nor did it reduce gastric juice output or pepsin activity. TDEYA significantly increased hexosamine content in gastric juice and the mucosa. The effect of TDEYA on HCl-ethanol or ethanol-induced gastric lesions may thus be exerted through its protective action on the stomach by secreting mucus and by not reducing gastric juice secretion or pepsin activity.     

Effect of the aqueous extract of xiao-ban-xia-tang on gastric emptying in mice

Gastric emptying effect of the aqueous extract of xiao-ban-xia-tang (XBXT) was investigated in mice. Mice with food deprived for 18 hours were orally administered a certain amount of test meal (ca. 0.8 g) equaling 0.8 ml in volume. The percentage of 0.8 g test meal remaining in the stomach after 20 minutes was estimated. It was shown that XBXT significantly antagonized dopamine- (0.56 mg/kg, i.p.), not atropine- (0.3 mg/kg, i.p.), induced gastric emptying inhibition. It also significantly restored metoclopramide-induced (8 mg/kg, p.o.) propulsion and potentiated methylneostigmine-induced (2 mg/kg, p.o.) gastric emptying enhancement. The aqueous extract of Pinellia ternata, but not that of Zingiber officinale showed an inhibitory activity on gastric emptying. The present results suggest that XBXT possesses a regulative effect on gastric motility.     

Antihepatotoxic activity of a quantified Desmodium adscendens decoction and d-pinitol against chemically-induced liver damage in rats

Ethnopharmacological relevance

The isolation of d-pinitol (or 3-O-methyl-d-chiro-inositol) from an aqueous decoction of Desmodium adscendens (Fabaceae) leaves and twigs is reported. The protective and curative effect of this decoction, in which d-pinitol was quantified, and of pure d-pinitol, against chemically-induced liver damage in rats has been evaluated.

Materials and methods

Enzyme levels of aspartate transaminase (AST), alanine transaminase (ALT) and alkaline phosphatase (ALP), which are among the usual biomarkers for liver damage, were determined in serum samples of experimental animals. The protective effects against d-galactosamine-induced and ethanol-induced liver damage of a decoction of D. adscendens, quantified on its main constituent d-pinitol, was investigated in rats. In addition, the curative effects of pure d-pinitol and D. adscendens against chronic d-galactosamine-induced and acute acetaminophen-induced hepatotoxicity in rats was studied. Silymarin was used as positive control.

Results

In a first experiment evaluating the protective effect against acute d-galactosamine-induced liver damage in rats, a significant decrease of AST and ALT was observed for the D. adscendens decoction at a dose equivalent to 5 mg/kg/day and 20 mg/kg/day d-pinitol, as well as 20 mg/kg/day pure d-pinitol. With respect to chronic ethanol-induced liver damage in rats, the protective effects of D. adscendens at doses equivalent to 2 mg/kg/day and 10 mg/kg/day d-pinitol, were not observed for serum AST and ALT levels. However, with respect to reduced mortality of animals, statistical analysis showed a trend towards significance for the Desmodium group receiving a dose equivalent to 10 mg/kg/day d-pinitol, versus the untreated hepatotoxic animals. Additional experiments in rat models of acute acetaminophen-induced and chronic d-galactosamine-induced liver damage indicated that D. adscendens decoction and pure d-pinitol had no curative effect when given in a dose equivalent to 10 mg/kg/day d-pinitol, or up to 20 mg/kg/day as a pure compound daily, respectively.

Conclusions

The aqueous decoction of D. adscendens showed a protective effect in rats against liver damage induced by d-galactosamine and ethanol, and this effect is at least in part due to the presence of d-pinitol. However, no curative effect of D. adscendens decoction or d-pinitol on liver damage induced by the tested chemicals could be demonstrated.     

Can the aqueous decoction of mango flowers be used as an antiulcer agent?

This study was designed to determine the effect of Mangifera indica flowers decoction, on the acute and subacute models of induced ulcer in mice and rats. A single oral administration of the aqueous decoction (AD) from M. indica up to a dose of 5 g/kg, p.o. did not produce any signs or symptom of toxicity in the treated animals. The oral pre-treatment with AD (250, 500 and 1000 mg/kg) in rats with gastric lesions induced by ethanol, decreased the gastric lesions from 89.0+/-6.71 (control group) to 9.25+/-2.75, 4.50+/-3.30 and 0, respectively. Pretreatment with AD (250, 500 and 1000 mg/kg) to mice with HCl/ethanol- or stress-induced gastric lesions resulted in a dose-dependent significant decrease of lesion index. In the piroxicam-induced gastric lesions, the gastroprotective effect of AD was reducing with the increase of the AD dose. In the pylorus-ligature, AD (p.o.) significantly decreased the acid output indicating the antisecretory property involved in the gastroprotective effect of M. indica. Treatment with AD during 14 consecutive days significantly accelerated the healing process in subacute gastric ulcer induced by acetic acid in rats. Pretreatment with N-nitro-l-arginine methyl ester (l-NAME), an inhibitor of NO-synthase, did not abolish the gastroprotective effects (99% with saline versus 80% with l-NAME) of AD against ethanol-induced gastric lesions. Pretreatment with N-ethylmaleimide (NEM), a blocker of endogenous sulphydryl group, significantly abolished the protective effects of AD against ethanol-induced gastric ulcers (95% with saline versus 47% with NEM). Phytochemical screening showed the presence of steroids, triterpenes, phenolic compounds and flavonoids. Estimation of the global polyphenol content in the AD was performed by Folin-Ciocalteu method and showed approximately 53% of total phenolic on this extract. These findings indicate the potential gastroprotective and ulcer-healing properties of aqueous decoction of M. indica flowers and further support its popular use in gastrointestinal disorders in Caribbean.     

竹节人参对小鼠乙醇性胃黏膜损伤的保护作用

目的：研究竹节人参对乙醇致小鼠胃黏膜损伤的保护作用,并对其机制进行探讨。方法：ICR雄性小鼠随机分为正常组,模型组,竹节人参低、中、高剂量组(1.5,3.0,6.0 g·kg^-1)和饮多安组(1.5 g·kg^-1),观察预防给予竹节人参对模型小鼠血清、尿液中乙醇含量的影响,测定小鼠血清、胃组织中谷胱甘肽过氧化物酶(GSH-PX),过氧化氢酶(CAT),超氧化物歧化酶(SOD)的活性及丙二醛(MDA),还原型谷胱甘肽(GSH)的含量；同时通过电镜观察小鼠胃黏膜超微结构病理变化。结果：竹节人参(1.5,3.0 g·kg^-1)可显著降低模型小鼠血清中乙醇含量,加快小鼠体内乙醇通过尿液排出；降低模型小鼠血清、胃组织中MDA含量,提高GSH含量及GSH-PX,CAT,SOD酶活性；同时保护小鼠胃黏膜上皮细胞线粒体结构完整以维持其功能正常。结论：竹节人参对乙醇性胃黏膜损伤有明显保护作用,其作用机制之一可能是通过抑制乙醇的胃肠吸收,促进乙醇通过尿、粪等直接快速排出体外,同时加强乙醇在胃肠道的首过代谢,提高机体的抗氧化能力,通过对胃黏膜上皮细胞线粒体结构的保护,可能是其发挥解酒护胃作用的机制之一。     

Gastric antiulcer and cytoprotective effect of Commiphora molmol in rats

The aqueous suspension of Commiphora molmol (oleo-gum resin) has been screened for its potential to protect gastric mucosa against the ulcers caused by 80% ethanol, 25% NaCl, 0.2 M NaOH, indomethacin and combined indomethacin-ethanol treatment. C. molmol pretreatment at doses of 250, 500 and 1000 mg/kg provided dose-dependent protection against the ulcerogenic effects of different necrotizing agents used. The effects caused by ethanol were further investigated. Treatment of rats with 1 ml of 80% ethanol was found to cause depletion of stomach wall mucus, reduction in the concentration of protein, nucleic acids and NP-SH groups in the stomach wall. Ethanol treatment also caused histopathological lesions including necrosis, erosion, congestion and haemorrhage of the stomach wall. Pretreatment with C. molmol offered a dose-dependent protection against all these effects. In the same manner it affected the malondialdehyde concentration altered by ethanol treatment. C. molmol also offered protection against mucosal damage caused by indomethacin and its combination with ethanol. The protective effect of C. molmol observed in the present study is attributed to its effect on mucus production, increase in nucleic acid and non-protein sulfhydryl concentration, which appears to be mediated through its free radical-scavenging, thyroid-stimulating and prostaglandin-inducing properties.     

Protective effect of a plant formula on ethanol-induced gastric lesions in rats

A plant formula and its five components were evaluated independently for their gastric protective effect against ethanol-induced stomach lesions in rats. Aqueous extracts of the plant formula (0.25-2g crude drug/kg orally) and its individual components (at the same dose) all showed significant stomach protective effects dose dependently. However, when these extracts were given to rats at a dose of 0.25 g/kg, the five single-herb preparations did not show any activity, but the formula-extract still exhibited a strong protective effect. These findings suggest the presence of a synergistic effect among the plant components. Chemical examination of the extracts indicated that the major ingredients of the five plants were essential oils, terpenoids, flavonoids, glycosides and saccharides and these may contribute to the stomach protective activity observed.     

Screening of some African medicinal plants for antiulcerogenic activity: Part 1

Aqueous extracts of six plant species used in Cameroonian ethnomedicine for the relief of stomach complaints were tested for antiulcer activity. An HCl/EtOH solution was used to induce gastric lesions in male Wistar rats (140–190 g). The extracts of Voacanga africana, Eremomastax speciosa, Emilia praetermissa, Spilanthes filicaulis and Centella asiatica produced complete mucosal cytoprotection at a dose of 1000, 190, 500, 2000 and 500 mg/kg respectively. The extract of Clerodendrum splendens (500, 750, 1000 mg/kg) showed significant (p <0.01) dose-dependent cytoprotection (50%–75%) compared with the controls. A combination of C. asiastica and S. filicaulis (1:1) (250–500 mg/kg) did not reveal the therapeutic advantage of combining both plant extracts in the clinical management of stomach ulcers. © 1997 John Wiley & Sons, Ltd.     

糖胃安煎剂对糖尿病胃肠病变大鼠Cajal间质细胞和P物质的影响

目的:研究糖胃安煎剂对糖尿病胃肠病变大鼠胃排空的影响,并探讨其作用机制。方法:2~3月龄Wistar雄性大鼠50只,10只大鼠作为正常组,另外40只腹腔注射50 mg·kg-1链脲佐菌素(STZ)复制1型糖尿病动物模型,尾静脉随机血糖≥16.9 mmol·L-1者为糖尿病大鼠模型建立,随机分为糖尿病模型组,糖胃安煎剂高、中、低剂量(15,10,5 g·kg-1)组,每组10只,正常组和糖尿病模型组给予等量蒸馏水灌胃。8周后取材,采用放射性核素单光子发射断层显像技术(SPECT)测定胃排空,免疫组织化学技术观察胃肌间层Cajal间质细胞变化,蛋白质免疫印迹法(Western blot)检测下丘脑P物质蛋白表达。结果:与正常组比较,糖尿病模型组大鼠的胃排空显著降低,胃肌间层阳性Cajal间质细胞显著降低,下丘脑P物质蛋白表达降低(P0.01)。与糖尿病模型组比较,糖胃安煎剂高、中剂量组的胃排空率升高,Cajal间质细胞升高,下丘脑P物质蛋白表达均升高(P0.05,P0.01);糖胃安煎剂低剂量组的胃排空率,Cajal间质细胞,下丘脑P物质蛋白表达有升高趋势,但无统计学意义。结论:糖尿病胃肠病变大鼠胃排空降低,胃肌间层Cajal间质细胞减少,下丘脑P物质表达降低,糖胃安煎剂可能通过增加大鼠胃肌间层Cajal间质细胞,提高下丘脑P物质水平,促进糖尿病胃肠病变大鼠胃排空,改善胃肠动力和功能。