Population genetic data on 15 autosomal STRs in a Pakistani population sample

Allele frequencies for the 15 STR loci (D3S1358, TH01, D21S11, D18S51, Penta E, D5S818, D13S317, D7S820, D16S539, CSF1PO, Penta D, VWA, D8S1179, TPOX and FGA) were investigated using PowerPlex^®16 amplification kit in a sample of 328 unrelated individuals from Islamic Republic of Pakistan. The allelic distributions were in Hardy Weinberg equilibrium for all loci except for the loci FGA, TPX and THO1. Various forensic indices were calculated and a comparison was made with geographically nearby populations.     

Forensic STR profile of two endogamous populations of Madhya Pradesh, India

Genotypic polymorphism studies at 15 highly polymorphic short tandem repeat (STR) loci were carried out in two populations belonging to one caste and one tribal group of Madhya Pradesh, in central region of India. These include 110 individuals from Brahmin caste (Kanyakubj) and 89 from Gond tribe (Ojha). The 15 loci studied are: 13 CODIS STR core markers, i.e., D8S1179, D3S1358, D21S11, D7S820, CSF1PO, vWA, TPOX, D18S51, THO1, D13S317, D16S539, D5S818, FGA and 2 other loci D19S433 and D2S1338. The results show departure from the Hardy-Weinberg equilibrium with respect to two loci, viz., D3S1358 and FGA in Gond tribe and at seven loci, viz., D21S11, D19S433, TPOX, D18S51, THO1, D5S818, and FGA in Brahmin caste. Population differentiation tests between the two studied populations and with seven neighboring populations (4 tribes and 3 castes - two middle castes and one Deshasth Brahmin) revealed significant differences at several loci. The power of discrimination of the microsatellite markers used was found to be high for both the populations. The data thereof is of immense significance for forensic result interpretation and is an addition to the existing autosomal STR database on Indian population.     

Population genetics of 29 autosomal STRs and 17 Y-chromosomal STRs in a population sample from Afghanistan

In this study, allele frequencies for 29 autosomal short tandem repeats (STRs) and haplotype frequencies for 17 Y-chromosomal STRs of an Afghan population have been generated. Samples from 348 men and women originating from Afghanistan were analysed for the autosomal STRs, and the combined match probability was estimated to be 7.5?×?10^?37. One hundred and sixty-nine men were analysed for the Y-chromosomal STRs, which resulted in 132 different haplotypes and a haplotype diversity of 0.995.     

Forensic features and genetic structure of 23 autosomal STRs in Artux Turkic-speaking population residing in southwestern Xinjiang Uyghur Autonomous Region

Autosomal short tandem repeats (STRs) are viewed as the gold standard in forensic individual identification and parentage testing. The Huaxia Platinum System contains 23 autosomal markers along with rs2032678 (Yindel) and amelogenin. Here, we genotyped 23-STRs in the southwestern Xinjiang Uyghur residing in the Artux Region and assessed the allele frequency and forensic statistical parameters. All investigated STRs are in conformity with the Hardy-Weinberg equilibrium with an effective combined power of discrimination (0.99999999999999999999999999992) and cumulative probability of exclusion (0.9999999997) in the Artux Uyghur population. Population comparisons among 54 worldwide populations via PCA and MDS indicate that the Artux Uyghur population has a close genetic relationship with geographically distinct Uyghurs and Kazakh groups than other East Asians or Eurasians. Genetic ancestry component dissection among 2198 individuals from Sinitic, Turkic, and Tibeto-Burman language groups further demonstrates the genetic homogeneity within the Turkic language family and apparent genetic heterogeneity among other language groups.

     

Genetic polymorphism of 20 autosomal short tandem repeats (STRs) in Himachal Pradesh population,India

International Journal of Legal Medicine - Genetic diversity indices and forensic parameters at 20 autosomal short tandem repeat (STR) loci were evaluated in 233 unrelated individuals of Himachal...     

Forensic effectiveness and genetic distribution of 20 non-CODIS STRs and D3S1358 in Guangdong Han population

In this study, Guangdong Han population was analyzed with Goldeneye™ DNA ID 22NC Kit containing 20 non-CODIS STR loci and a CODIS STR locus. In 503 unrelated individuals, a total of 232 alleles and 813 genotypes were observed. The values of polymorphism information content (PIC), expected heterozygosity (He), and observed heterozygosity (Ho) ranged from 0.6678 to 0.8618, 0.7197 to 0.8757, and 0.6938 to 0.8767, respectively. No deviation from Hardy-Weinberg equilibrium was observed at all loci after the Bonferroni correction. The combined power of discrimination and the combined power of exclusion were 0.999999999999999999999999937051 and 0.999999998467033, respectively. Population comparison revealed that Guangdong Han population was relatively close to Huadong Han population. These results suggested that the 21 STR loci are highly polymorphic and Goldeneye™ DNA ID 22NC Kit is suitable for individual identification and paternity testing in Guangdong Han population.     

Forensic and phylogenetic characterization of 15 autosomal STRs in Hazara population of Pakistan

In the current study, 217 unrelated individuals of the Hazara population were genotyped for 15 autosomal short tandem repeats to generate parentage and forensic efficacy parameters. Hazaras belong to the Shi’a sect and are recognized by their Turko-Mogholi features. We found that D2S1338 was the most discriminatory locus with a maximum power of exclusion and high value of polymorphism information content. Whilst the Combined Power of Discrimination (CPD), Combined Matching Probability (CMP) and Combined Power of Exclusion (CPE) were 0.999999999999999, 2.76796338879E^-17 and 0.999999040733479 respectively. Furthermore, the pattern of genetic affinity with genetically assumed related populations was demonstrated through Heat Map and Phylogenetic analysis, which revealed a great level of genetic closeness of Hazaras with Mongol population and descendants of Genghis Khan. The resulting data can be used for forensic applications and anthropological studies.     

Molecular characterization and forensic relevance of the autosomal STRs for the population of North Indian geographical province Himachal Pradesh,India

Keeping in view the diverse demography of India, present study was undertaken to explore the molecular characterization and forensic relevance of 20 autosomal STRs for the highly diverse population of north Indian state Himachal Pradesh. 724 unrelated individuals from the admixed population of Himachal Pradesh were undertaken for present study and 20 autosomal STRs used to explore genomic diversity of studied population. A total of 270 different alleles along with 13.5 alleles per locus were observed. The allele 8 of the locus TPOX was observed as the most frequent allele. Observed heterozygosity ranged from 0.677 to 0.898, which supported wide range of selection of the unrelated individuals for this study. Combined power of discrimination, power of exclusion, matching probability and paternity index were observed as 1, 0.9999999958, 3.9 × 10⁻²⁶ and 2.3 × 10⁸ respectively, across the studied loci. In the population differentiation test, studied population showed genetic relatedness with Indian population rather than the populations of West, North and North east countries. Present study deciphered the novel autosomal STR data, which could be useful for the forensic application and population genetic studies.     

Haplotype data for 17 Y-STR loci in the population of Madhya Pradesh,India

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Evaluation of genetic polymorphism at 20 autosomal STR loci in Rajput population of Himachal Pradesh,India

In the present study, genetic diversity indices and forensic parameters of Rajput population of Himachal Pradesh, India, were obtained at 20 autosomal short tandem repeat (STR) loci included in the PowerPlex® 21 System (Promega, USA). With 227 alleles and 11.35 ± 0.89 alleles per locus, none of the locus deviated from the Hardy–Weinberg equilibrium (HWE). The most polymorphic and discriminative locus was Penta E. Phylogenetic tree showed high genetic affinity of Rajput population of Himachal Pradesh with Rajputs of Madhya Pradesh and Yadav of Bihar. Overall, the tested 20 autosomal STRs were polymorphic in Rajput population and found suitable in forensic casework and population genetic studies.     

Allele frequency distribution for 15 autosomal STR loci in Afridi Pathan population of Uttar Pradesh,India

Allele frequencies of the 15 autosomal short tandem repeat (STR) loci D8S1179, D21S11, D7S820, CSF1PO D19S433, vWA, TPOX, D18S51, D3S1358, THO1, D13S317, D16S539, D2S1338, D5S818 and FGA were determined in Afridi Pathan population of Uttar Pradesh, India. All the 15 STR loci studied were found to be highly polymorphic with respect to observed heterozygosity values. Adherence to the expectations of the Hardy–Weinberg equilibrium (HWE) was confirmed for all the loci with an exception of TPOX and FGA. The allele 12 in CSF1PO was found to be most frequent. The power of discrimination was found to be high ranging from a minimum of 0.858 for the locus CSFIPO to maximum of 0.962 for the locus FGA, thereby facilitating the validation and efficiency of these STR markers in human identification. Population differentiation test between the studied and neighboring populations revealed significant differences at several loci suggesting the endogamous nature of the studied population. To the best of our knowledge, Afridi Pathan population has not been explored genetically for generating forensic data on STR markers. Therefore, STR allele frequency data of this unique population is a valuable contribution to the existing DNA database on Indian populations.     

Population data for 15 autosomal STRs loci and 12 Y chromosome STRs loci in a population sample from the Sardinia island (Italy)

One hundred twenty-five unrelated individuals (69 females and 56 males) from Sassari (Northern Sardinia) and Orgosolo (Central Sardinia) were typed for 15 STRs loci. The 56 males were typed for 12 Y chromosome STRs loci too. Frequency distribution is described.     

Allelic frequency database of 15 polymorphic autosomal STRs in the Malayalam-speaking population of Kerala,India

International Journal of Legal Medicine - In this study, we assessed and established an allelic frequency database of Malayalam-speaking population of south western Indian state Kerala, using 15...     

Revealing genomic history and forensic features of Gurjars from western Uttar Pradesh and National Capital Region Delhi using 23 autosomal STRs

Here we report the genomic history of Gurjars and framed the useful set of autosomal STRs for Gujjar population. We designed this study with a total number of 215 Gurjars from district Saharanpur (previously known as Gujarat due to presence of many Gujjar zamindars), Moradabad, Bulandshahr, Ghaziabad, Meerut, Noida and NCR Delhi. Locus SE33 was found the most polymorphic and discriminating marker for Gujjar population while locus TPOX is the least. Ancestral information of Gurjars was revealed by comparing the Gujjar’s population data with 19 neighbouring populations. In Neighbor Joining (NJ) tree Gurjars were found closer to Gujjars of Jammu region, population of Rajasthan and Uttarkhand, due to the same stock of gene pool.     

Genomic portrait of population of Jharkhand,India, drawn with 15 autosomal STRs and 17 Y-STRs

International Journal of Legal Medicine - We analysed 15 autosomal STRs in 200 unrelated individuals (102 males and 98 females) and 17 Y-STRs in 102 unrelated males living in Jharkhand, India, to...     

Allele distribution of three STRs in a population from Mozambique

The short tandem repeat systems (STRs) D12S391, HUMFIBRA/FGA and HUMACTBP2 (SE33) were studied in a population of unrelated individuals from the Maputo area of south Mozambique. Received: 4 September 1998 / Received in revised form: 10 February 1999     

Allele frequency distribution at 15 autosomal STR loci in Panggi, Komkar and Padam sub tribes of Adi, a Tibeto-Burman speaking population of Arunachal Pradesh, India

Fifteen autosomal STR loci were analyzed in 223 healthy individuals belonging to three remote, isolated Tibeto-Burman speaking sub tribes namely, Panggi, Komkar and Padam of Adi tribe of Arunachal Pradesh, India. The analyzed markers exhibited a high degree of polymorphism in the studied populations. Statistical parameters of forensic interest; observed heterozygosity, probability of homozygosity, exact test, likelihood ratio test, power of discrimination, power of exclusion, match probability and typical paternity index were determined for all loci. The average heterozygosity values were found to be low in the three populations (Panggi: 0.7747; Komkar: 0.7742 and Padam: 0.7663). The combined power of discrimination and power of exclusion were 0.9999 in the studied populations thereby revealing the high forensic significance of the chosen markers. The study indicates the utility of the tested microsatellite markers in forensic human identification, paternity testing and human population genetic studies.     

Allele frequencies for 15 autosomal STRs in a population sample from the Federal District (Brazil)—a territory that arose from nothing

The Federal District (Brazil) was created in 1960 in the Central-West Region of Brazil in a previously unpopulated area. In 2010, this artificially founded district was populated by 2,562,963 inhabitants. In this study, the genetic variations of the 15 Next Generation Multiplex (NGM^TM) short tandem repeat loci were analyzed. The results indicate that the NGM^TM is a highly informative genetic system in this population, which is more similar to the southeastern, northeastern, and overall Brazil populations. This conclusion agrees with the population composition reported in the 2010 National Survey Inquiries, in which most of the immigrants were from the northeast and the southeast.     

Genetic variation of 20 autosomal STRs in Jats belonging to Delhi,India

International Journal of Legal Medicine - The present study based on 20 autosomal STR polymorphism reveals the genetic diversity of the Jat population of Delhi, India (n = 120). A total of 240...     

Haplotype diversity of 23 Y-chromosomal STRs in a population sample from the Federal District (Brazil)—a territory that arose from nothing

In this study, the genetic variations of 23 short tandem repeats on the Y-chromosome were analyzed in a sample of 201 males from the Federal District (Brazil). The Federal District (Brazil) was built in 1960 in Brazil’s Central West region, where there was no previous population. In 2010, the population of this artificially founded district consisted of 2,500,000 inhabitants. We observed 200 different haplotypes, 199 of which were unique and one of which occurred two times. The haplotype diversity was 0.9999, and the discrimination capacity was 0.995. The data are available in the Y chromosome haplotype reference database under accession number YA003843. The results were compared to the haplotypes from other Brazilian macroregions.