儿童50 颗恒牙再植后的回顾性研究

目的:分析再植牙的预后及影响预后的相关因素,观察再植后牙根吸收出现时间及影响因素.方法:对2000～2006 年于北京大学口腔医学院儿童口腔科注册的系统病历中,观察期≥6 个月的全脱位牙再植病历进行回顾性研究,记录患者及再植牙信息.应用卡方检验和生存分析(cox regression)进行统计学分析.结果:符合纳入标准的外伤病历39 份,涉及再植牙50 颗.牙周膜愈合的发生率为18%,牙根吸收发生率为82%,替代性吸收是最常见的牙周组织预后形式(64%).牙根发育情况和保存方式与牙周组织预后具有显著相关性;牙根发育情况、牙髓治疗时机可以影响牙根吸收出现的时间(P<0.05).结论:替代性吸收是再植牙最常见的牙周组织预后形式.再植牙牙根发育情况和保存方式与牙周组织预后具有显著相关性.牙根发育情况、牙髓治疗时机可以影响牙根吸收出现的时间.     

Effect of immediate calcium hydroxide treatment and permanent root-filling on periodontal healing in contaminated replanted teeth

Abstract – The effect of immediate permanent root-filling and immediate intracanal calcium hydroxide treatment on experimentally contaminated and necrotized periodontal membranes (PDM) in avulsed and subsequently replanted monkey teeth was studied. Almost the entire root surface in the non-endodontically treated control teeth were covered with inflammatory resorp-tion, A significant shift towards ankylosis not associated with root resorption (> 80% of the total root surface area) was noted following calcium hydroxide treatment. About two thirds of the root surfaces from the teeth with permanent root fillings showed surface resorptions or ankylosis preceded by root resorption. The root surface area which would have been covered by inffammatory resorption, unless endodontic treatment had been implemented, were instead predominantly covered by either surface resorption (permanent root filling) or ankylosis (calcium hydroxide treatment), Ankylosis compared with surface resorption may in the long term lead to progressive loss of root substance due to replacement resorption. It was concluded that calcium hydroxide may be an excellent initial intracanal treatment in teeth with healthy PDM, but care should be taken not to risk unnecessary development of ankylosis by prolonged treatment of teeth with compromised PDM.     

Replantation of 400 avulsed permanent incisors. 1. Diagnosis of healing complications

Abstract A material of 322 patients with 400 avulsed and replanted permanent teeth were followed prospectively in the period from 1965 to 1988 (mean observation period=5.1 yrs). The age of the patients at the time of replantation ranged from 5 to 52 yrs (mean =13.7 yrs and median =11.0 yrs). Standardized patient records were used through the entire period in order to obtain valid data concerning the extent of injury and treatment provided. At the follow–up period, pulpal and periodontal healing were monitored by clinical examination, mobility testing and standardized radio–graphic controls. Thirty–two of the replanted teeth (8%) showed pulpal healing. When related to teeth with incomplete root formation, where pulpal revascularization was anticipated (n = 94) the frequency of pulpal healing was 34%. Periodontal ligament healing (i.e. with no evidence of external root resorption) was found in 96 teeth (24%). Gingival healing was found in 371 teeth (93%). During the observation period, 119 teeth (30%) were extracted. Tooth loss was slightly more frequent in teeth with incomplete root formation at the time of replantation than in teeth with completed root formation.     

The effect of extra-alveolar root filling with calcium hydroxide on periodontal healing after replantation of permanent incisors in monkeys

The effect of calcium hydroxide, used as an extra-alveolar root filling material, on periodontal healing was examined in green vervet monkeys (Cercopithecus aethiopa). Incisors were extracted and a root canal filling of calcium hydroxide paste was processed. Control teeth were replanted after either pulp extirpation or root filling with gutta-percha. In the experimental groups, the extra-alveolar period was either 18 or 120 minutes. The animals were killed eight weeks after replantation, and the replanted teeth were examined histologically. The histologic parameters recorded for each tooth were surface resorption, inflammatory resorption, replacement resorption (ankylosis), periapical inflammatory changes, and downgrowth of pocket epithelium. Teeth with canals filled with calcium hydroxide that had an extra-alveolar time period of 18 minutes, showed noticeably more replacement resorption than either the teeth with canals filled with gutta-percha or those with extirpated pulps. Calcium hydroxide paste may diffuse through the apical foramen, thus injuring the periodontal ligament in the apical region. Consequently, the use of calcium hydroxide does not seem justified in the initial treatment of avulsed teeth, but it can be used after some weeks when periodontal ligament healing has progressed.     

Replantation of permanent incisors in children using Emdogain®

Abstract –  The aim of this study was to determine whether application of an enamel matrix protein derivative, Emdogain^® (Biora AB Malmo, Sweden) to the root surface of avulsed permanent incisors would improve postreplantation outcomes in a pediatric population. Between June 1999 and May 2002, 25 avulsed permanent maxillary incisors (22 centrals and three laterals) were treated with Emdogain^® and followed for up to 32 months, mean duration 20.6 months (range: 6.9–32.5 months). Mean patient age at the time of treatment was 12.0 years (range: 7.7–17.6 years) and mean extra-alveolar duration was 185 min (range: 100–300 min). At the end of their follow-up each of the replanted incisors demonstrated radiographic evidence of replacement root resorption and clinical evidence of ankylosis. None of the replanted teeth were affected by inflammatory root resorption and there was no evidence of infection. When compared with the control samples from Barrett and Kenny ( Endod Dent Traumatol 1997;15:269–72.) and Andersson et al. ( Endod Dent Traumatol 1989;5:38–47.) this sample treated with the Emdogain^® protocol demonstrated significantly less root resorption than either of the control samples ( anova , P  < 0.0001). Although the Emdogain^® protocol did not produce periodontal regeneration, it did eliminate inflammatory resorption and infection and led to significantly less root resorption compared with the two historical controls.     

Replantation of 400 avulsed permanent incisors. 4. Factors related to periodontal ligament healing

Abstract 400 avulsed and replanted permanent teeth were examined for periodontal ligament (PDL) healing, using standardized radiographic and clinical examination procedures (i.e. percussion test and mobility test). The effect of various clinical factors was examined, such as age and sex of the patient, type of tooth replanted, presence of crown fracture or bone fracture, stage of root development (including apical diameter and length of the pulp), type and length of extra-alveolar storage, clinical contamination of the root surface, type of root surface cleansing procedure, type and length of splinting period and antibiotic therapy. Surface resorption was generally diagnosed after 12 months; while inflammatory resorption and replacement resorption (ankylosis) were usually observed after 1 month and 12 months respectively. Most resorptive processes were diagnosed within the first 2–3 years. However, although rarely, even after 5 and 10 years new resorptive processes could be diagnosed. A univariate statistical analysis of 272 teeth revealed 9 factors significantly related to PDL healing. A subsequent multivariate analysis revealed that the following 4 factors had the strongest impact upon PDL healing, in descending order of significance: Stage of root development; length of the dry extra-alveolar storage period; immediate replantation and length of the wet period (saliva or saline storage). Nonphysiological storage, such as homemade saline and sterilizing solutions (chloramine and alcohol) always led to root resorption. Storage in tap water for more than 20 minutes usually led to root resorption. The common denominator for all these factors related to PDL healing appears to be survival of the PDL cells along the root surface. Based on these findings, immediate replantation is recommended irrespective of stage of root development.     

Splinting duration and periodontal outcomes for replanted avulsed teeth: a systematic review

Abstract –  Clinical guidelines are now available for the management of avulsed teeth. The principles of evidence-based dentistry can be used to assess whether these guidelines are based on currently-available evidence. A qualitative systematic review was conducted of relevant clinical literature to examine the evidence on splinting duration and periodontal healing outcomes . The review was constrained markedly by small sample sizes, retrospective nature of clinical audits, dissimilarities of selected studies in their design, methodology and observation periods, and lack of uniformity in terminology for outcomes. A total of 138 replanted avulsed permanent teeth pooled from four papers each reporting both short-term splinting (14 days or less) and long-term splinting (over 14 days) in accord with current clinical guidelines, were studied. The evidence for an association between short-term splinting and an increased likelihood of functional periodontal healing, acceptable healing, or decreased development of replacement resorption, appears inconclusive. The study found no evidence to contraindicate the current guidelines and suggests that the likelihood of successful periodontal healing after replantation is unaffected by splinting duration. Pending future research to the contrary, it is recommended that dentists continue to use the currently-recommended splinting periods when replanting avulsed permanent teeth.     

Replantation and transplantation following avulsion of two maxillary incisors

Abstract –  Here we describe replantation and transplantation as a treatment conducted in a 16-year-old patient following a bicycle accident. An avulsed left central incisor in maxilla was replanted after extra-oral root canal treatment, and the lost right central incisor of the maxilla was replaced by a left lateral incisor of the mandible in the region of the fracture fissure. Follow-up examination at 34 months showed validity of treatment, although slight evidence of replacement resorption in the transplanted and replanted teeth was revealed.     

A prospective study of factors affecting survival of replanted permanent incisors in children

Objective . To examine the factors associated with the onset of resorption in replanted avulsed permanent incisor teeth and to demonstrate their patterns of survival free of resorption.
Setting . Department of Paediatric Dentistry, Belfast.
Design . Prospective recording of the timing of the injury and of replantation, the storage media, the degree of root development and contamination, and the completion of root treatment. Clinical and radiographic reviews at 3-month intervals. Root resorption classified as replacement and inflammatory. Logistic regression and survival curves with freedom from resorption as outcomes.
Results . In 50 avulsed incisors in children aged 6–16 years the best predictor of overall resorption was total time of dryness, and that for replacement resorption was total extra-oral time. The timing of detection of resorption varied from 102 days to 997 days.
Conclusion . Both total extra-oral time and time stored dry are important factors for the onset of resorption in replanted avulsed teeth in children.     

Replantation of 400 avulsed permanent incisors. 3. Factors related to root growth

Abstract Four hundred avulsed and replanted permanent incisors were examined for pulpal and periodontal healing. In 30 teeth, root formation was incomplete at the time of injury. Two teeth were excluded from the study due to nonphysiological extraalveolar storage (i.e. homemade saline). Of the 28 remaining replanted incisors, 7 showed subsequently completed root development, 8 partially completed root development and 13 arrested root development. Completed root development subsequent to replantation was found to be significantly related to pulpal revascularization, being rare in cases with pulp necrosis (5 of 15 teeth) and frequent after pulpal healing (11 of 13 teeth) (p=0.01). Root development was not found to be significantly related to the extraalveolar storage period; but occurred slightly more frequently when the dry–storage period was less than 45 min. (p=0.13). Ingrowth of bone and formation of an internal periodontal ligament (PDL) was found in 6 teeth and was related to arrested root formation in cases with pulpal healing. The explanatory factor for these findings appeared to be damage to the Hertwig's epithelial root sheath.     

Apexification of a replanted tooth using mineral trioxide aggregate

Abstract –  The most important factors determining periodontal healing after replantation of an avulsed tooth are the extra oral period and the media in which the tooth is preserved before replantation. This case report describes an adequate periodontal healing of an avulsed immature tooth replanted after 20 min of extra alveolar dry time. Vitality was not regained and after disinfection of the pulp space mineral trioxide aggregate was used as the root filling. Follow-up confirmed complete healing periradicularly.     

Periodontal healing of exarticulated monkey teeth stored in milk or saliva

Abstract – Saliva has usually been recommended as a storage medium for exarticulated teeth. Recent tissue culture studies have, however, shown that milk has a more suitable osmolaliry than saliva and that milk is a bettter storage medium for human periodontal ligament cell. In the present investigation periodontal healing of replanted monkey teeth has been studied after storage of teeth in milk or saliva before replantation. There was much less root resorption, especially inflammatory resorption, after storage in milk thatn in saliva. Storage of teeth in milk for 3 h before replantation resulted in the same low frequence of root resorptions as was seen after immediate replantation. There was hardly any replacement resorption (ankylosis) seen in the replanted teeth.     

Periodontal healing of replanted dog teeth stored in Viaspan, milk and Hank's balanced salt solution

Abstract This study examined, histologically, periodontal healing and root resorption of replanted dogs teeth stored in Viaspan for different time periods and compared these healing patterns to those after storage in milk or Hank's balanced salt solution (HBSS). Seventy-two beagle incisors were endodontically treated, extracted, randomly divided and placed in Viaspan or milk for 6, 12, 24 and 36 h, and Viaspan or HBSS for 36, 48, 72 and 96 h, after which they were replanted. Four negative control teeth were immediately replanted while four positive controls were allowed to dry for one hour before replantation. All replanted teeth were splinted for 14 days. Two months after replantation the clogs were killed and the teeth histologically examined for healing of the supporting tissues. For Viaspan neither replacement nor inflammatory root resorption was seen after 6 and 12 h storage. A statistically significant rise in the incidence of replacement resorption was seen at 24, 36 and 48 h which decreased again at 72 and 96 h to levels equal to storage for 6 and 12 h. The occurrence of inflammatory root resorption was low and significantly increased only at 48 h after which it decreased significantly again. Viaspan proved superior to milk as a storage medium. Teeth stored in HBSS showed healing results similar to those stored in Viaspan.     

Replantation of three avulsed permanent incisors with complicated crown fractures

AIM: To report a rare case of multiple avulsions of permanent teeth associated with complicated crown fractures. SUMMARY: The case describes a 3-year follow-up of the treatment of three avulsed maxillary incisors with complicated crown fractures. The teeth were replanted after an extra-oral time of 90 min (extra-oral dry time 6 min). After root canal treatment, the fractured maxillary incisors were restored with a layered hybrid composite material to re-establish aesthetics and function. Follow-up visits showed signs of replacement resorption affecting the maxillary central incisors but only minor signs of root resorption on the maxillary right lateral incisor. These findings were supported by digital volume tomography, which was performed 18 and 36 months post-trauma in order to assess extent, severity and progression of root resorption more accurately. *Healing of avulsed teeth depends on the degree of periodontal ligament (PDL) damage. *Digital volume tomography might improve diagnostics of root resorption.     

促进撕脱性损伤牙延期再植后牙周愈合的研究进展#br#

牙撕脱性损伤是牙外伤中预后较差的一种,目前对于恒牙撕脱性损伤的治疗一般采用牙再植术,再植时根面牙周组织活性对其预后至关重要。临床调查显示,撕脱性损伤牙再植多为延期再植,离体牙根面牙周膜在干燥状态下脱水坏死是导致术后病理性牙根吸收的主要原因。国际牙外伤协会建议在延期再植时采取适当措施,促进再植牙的牙周愈合再生,减少病理性吸收,延长再植牙使用寿命。文章结合2020版国际牙外伤协会撕脱性损伤牙治疗指南对现有促进撕脱性损伤牙延期再植后牙周愈合相关研究做一综述,包括根面处理、药物及干细胞治疗在牙再植术中的应用。     

Periodontal healing by periodontal ligament cell sheets in a teeth replantation model

ObjectiveSuccessful transplantation of avulsed teeth is to restore the attachment and regenerate the periodontal support. Different strategies have been applied in treatment from modification of teeth storage, antibiotic usage to peridontium tissue replacement. We developed a novel periodontal ligament cell-sheet delivery system to apply on delayed replanted teeth in promoting periodontal healing in a canine model.DesignAutologous periodontal ligament (PDL) fibroblasts were isolated from extracted premolars of beagle dog. The cell-sheets were fabricated using normal culture dish after stimulation of extracellular matrix formation. Teeth were surgically extracted and attached soft tissues were removed. After root canal treatment, the root of teeth were wrapped by the PDL cell-sheets and replanted back to prior socket accordingly whilst teeth without cell sheets as a control. Eight weeks after surgery, the animals were sacrificed and decalcified specimens were prepared. Regeneration of periodontal tissue was evaluated through histology assay.ResultsMulti-layered PDL cell-sheet could be attached on tooth root and most cells on sheet-tooth constructs were viable before replantation. Minimum clinical signs of inflammation were observed in experiment. PDL cell-sheets group show significant higher occurrence of favourable healing (88.4%) than control group with low healing (5.3%). Periodontal ligament and cememtum tissue regeneration was observed in the experimental group, and the regenerated tissues showed high collagen type III, type I and fibronectin expression.ConclusionThe periodontal ligament cell-sheets fabricated through normal cell culture dish has a potential for regeneration of periodontal ligament and may become a novel therapy for avulsed teeth replantation.     

Esthetic rehabilitation of avulsed–replanted anterior teeth: a case report

Abstract –  Avulsion is a complex injury affecting the pulp, periodontal ligament and the alveolar bone. Avulsed permanent teeth can survive following replantation. However, post-traumatic external root resorption eventually resulting in loss of the traumatized tooth is a frequent finding. After replantation of the avulsed teeth, esthetic requirements can be needed. Treatment options include porcelain laminate veneers, metal-ceramic restorations and all-ceramic crowns as well as minimally invasive procedures such as direct resin composite bonding. This article describes the restoration of avulsed and replanted teeth with direct resin composite laminate veneers. Because of the fact that lost fragments were recovered with the loss of anterior esthetic, and bearing in mind the patient's psychologically affected, we considered direct resin composite laminate veneer restoration of the avulsed and replanted teeth as the best therapeutic option. The patient was satisfied with the final result.     

年轻恒牙完全脱位再植后疗效分析

目的：评价年轻恒牙完全脱位再植后的愈合方式,对不同发育阶段年轻恒牙再植术后的疗效进行总结,为更好保留患牙的美观与功能提供参考。方法回顾分析30颗完全脱位年轻恒前牙,根据国际牙外伤协会治疗指南进行治疗。评价再植后年轻恒牙牙周组织愈合方式,分为牙周膜愈合、表面吸收愈合、替代性吸收（骨粘连）、炎性吸收。结果30颗完全脱位的年轻恒牙再植后,其中7颗为牙周膜愈合,占23.3%;1颗为表面吸收愈合,占3.3％;13颗为替代性吸收,占43.3%;9颗为炎性吸收,占30.0％。结论年轻恒牙发生完全脱位,再植后愈合方式受多种因素影响。当出现不良的愈合方式限制颌面部发育时,一定要及时干预,减少患者生理和心理的创伤。     

The effect of Emdogain® and 24% EDTA root conditioning on periodontal healing of replanted dog's teeth

Abstract –  Controversies still exist as for the regenerative role of enamel matrix derivatives and the need for removal of the periodontal ligament in replanted teeth. The purpose of this study was to evaluate the effect of Emdogain^® and 24% ethylenediamine tetraacetic acid (EDTA) root conditioning on periodontal healing of replanted dog's teeth. Teeth were extracted, endodontically treated and preconditioned as follows: group 1, Emdogain^®; group 2, Emdogain^® + EDTA and group 3, EDTA. Teeth were replanted after 30 min extraoral time, splinted for 15 days and animals sacrificed after 8 weeks of observation. Histological evaluation was performed using hematoxylin/eosin and Masson trichrome and results scored based on previously reported criteria for histological evaluation. Replacement root resorption was histologically diagnosed in all groups except in the negative control. A parametric analysis showed no statistically significant differences between experimental groups. Root preconditioning with Emdogain^® alone or in combination with 24% EDTA showed no evidence of regeneration of collagen fibers and consequently did not prevent the development of replacement root resorption on replanted dog's teeth.     

Effect of immediate calcium hydroxide treatment and permanent root-filling on periodontal healing in contaminated replanted teeth

The effect of immediate permanent root-filling and immediate intracanal calcium hydroxide treatment on experimentally contaminated and necrotized periodontal membranes (PDM) in avulsed and subsequently replanted monkey teeth was studied. Almost the entire root surface in the non-endodontically treated control teeth were covered with inflammatory resorption. A significant shift towards ankylosis not associated with root resorption (greater than 80% of the total root surface area) was noted following calcium hydroxide treatment. About two thirds of the root surfaces from the teeth with permanent root fillings showed surface resorptions or ankylosis preceded by root resorption. The root surface area which would have been covered by inflammatory resorption, unless endodontic treatment had been implemented, were instead predominantly covered by either surface resorption (permanent root filling) or ankylosis (calcium hydroxide treatment). Ankylosis compared with surface resorption may in the long term lead to progressive loss of root substance due to replacement resorption. It was concluded that calcium hydroxide may be an excellent initial intracanal treatment in teeth with healthy PDM, but care should be taken not to risk unnecessary development of ankylosis by prolonged treatment of teeth with compromised PDM.