皮肤淋巴细胞相关抗原在寻常性银屑病中的表达

目的 探讨皮肤淋巴细胞相关抗原(CLA)在寻常性银屑病发病过程中的表达情况.方法 选择寻常性银屑病患者70例,其中进展期20例,静止期50例,采用免疫组化法和流式细胞分析法检测银屑痛皮损及外周血中CLA的表达情况.结果 银屑病皮损中CLA及其配体E-选择素表达均为强阳性.银屑病进展期患者外周血表达CLA的T细胞亚群明显高于正常人,同时对比静止期也明显增高.而静止期与正常人比较只有CLA+CD8+T细胞显著升高.结论 CLA在T细胞上的表达与银屑病病理机制有关.     

流式细胞术双色免疫荧光标记观察寻常性银屑病患者外周血淋巴细胞亚群

目的了解寻常性银屑病患者外周血淋巴细胞亚群的变化。方法应用流式细胞仪双色免疫荧光标记,检测98例寻常性银屑病患者外周血淋巴细胞亚群并与102名正常人进行比较。结果银屑病患者外周血CD4+细胞百分数显著高于正常人对照组(P<0.05);NK细胞百分数显著低于正常人对照组(P<0.01),寻常性进行期银屑病患者T细胞和CD4+细胞显著高于静止期银屑病患者(P<0.05),进行期银屑病患者T细胞、B细胞、CD4+细胞百分数均显著高于正常人对照组(P<0.001),NK细胞显著低于正常人对照组(P<0.001)。静止期银屑病患者与正常人对照组相比淋巴细胞亚群各项指标无明显差异。结论寻常性银屑病患者外周血淋巴细胞亚群的变化主要表现在T、B淋巴细胞特别是CD4+细胞的升高和NK细胞的降低。     

173例寻常型银屑病患者外周血T淋巴细胞亚群、免疫球蛋白及补体水平与PASI相关性分析

目的：探讨寻常型银屑病患者外周血T淋巴细胞亚群、免疫球蛋白及补体的水平变化及临床意义。方法：对173例银屑病患者进行PASI评分,采用流式细胞仪及全自动酶标仪对患者组和150例正常人外周血T淋巴细胞亚群、IgM、IgG、IgA及补体C3、C4的表达水平进行检测和分析。结果：寻常型银屑病患者外周血CD8^＋T细胞数量显著高于对照组（P〈0．05）,且与患者PASI呈正相关（r=0．845,P〈0．01）;补体C3较对照组显著降低（P〈0．05）,且与患者PASI呈负相关（r=0．699,P〈0．01）。而CD3^＋及CD4^＋T细胞、IgG、IgM、IgA及补体C4水平与对照组相比差异无统计学意义（P〉0．05）。结论：寻常型银屑病患者外周血免疫功能处于紊乱状态,CD8^＋T细胞百分率及补体C3水平与银屑病患者的病情密切相关。     

寻常型进展期白癜风患者外周血 CD4+CD25+调节性T细胞检测

目的：探讨寻常型进展期白癜风患者外周血T细胞亚群及CD4^＋CD25^＋调节性T细胞（Treg）的水平变化及其意义。方法：采用流式细胞分析技术对45例寻常型进展期白癜风患者和24例正常人外周血CD4^＋、CD25^＋、Foxp^3＋,CD^4＋CD25^＋,CD4^＋CD25^＋Foxp3^＋T细胞水平。结果：寻常型进展期白癜风患者外周血CD4^＋CD25^＋Treg和CD4^＋CD25^＋Foxp3^＋nTreg细胞数量显著高于正常对照组（P〈0．05）。结论：寻常型进展期白癜风患者外周血Treg水平发生变化，使体内免疫功能处于失衡状态，细胞免疫尤其是Treg可能参与了白癜风的发病。     

糖皮质激素上调系统性红斑狼疮患者外周血CD4＋CD25＋T细胞CD62L的表达

目的探讨糖皮质激素（简称激素）治疗对系统性红斑狼疮（SEE）患者外周血CD4^＋CD25^＋T细胞（调节性T细胞）亚群CD62L表达的影响。方法采用流式细胞仪检测了10例初发未经治疗的SLE患者和25例经激素治疗后的SLE患者外周血CD4^＋CD25^＋T细胞亚群CD62L的表达，并与12例正常人对照组相比较。结果未经治疗的初发SLE患者CD62L^＋和CD62L^-的CD4^＋CD25^＋T细胞均较健康对照组明显减少。激素治疗后，SLE患者外周血CD4^＋CD25^＋CD62L^＋T细胞显著升高。而且与糖皮质激素剂量有关。大剂量激素治疗组CD4^＋CD25^＋CD62L^＋T细胞显著高于未治疗组和正常人对照组，但小剂量激素治疗组CD4^＋CD25^＋CD62L^＋T细胞仅高于未治疗组。然而无论小剂量激素治疗组还是大剂量激素治疗组的CD4^＋CD25^＋CD62L^-T细胞与正常人对照组比较差异均无统计学意义。SLE患者外周血CD4^＋CD25^＋CD62L^＋T细胞与疾病活动指数成负相关。结论糖皮质激素可能上调SLE患者外周血CD4^＋CD25^＋CD62L^＋T细胞的生成，促进外周免疫耐受，从而缓解病情。     

红斑丘疹及鳞屑性皮肤病

20 0 4 15 80　流式细胞术双色免疫荧光标记观察寻常性银屑病患者外周血淋巴细胞亚群 /王禾 (北京中医医院皮肤科 )… //中国中西医结合皮肤性病学杂志 .- 2 0 0 3,2 (4 ) .- 2 2 5～ 2 2 6检测结果显示 ,银屑病患者外周血 CD 4 细胞百分数显著高于正常人对照组 ,NK细胞百分数显著低于对照组 ,进行期 T细胞和 CD 4 细胞高于静止期 ,进行期患者 T细胞、B细胞、CD 4 细胞百分数高于对照组 ,NK细胞低于对照组 ,静止期各项与对照组比较无差异。提示银屑病患者外周血淋巴细胞亚群的变化主要表现在T、B淋巴细胞 ,特别是 CD 4 细胞的升…     

尖锐湿疣患者皮损及外周血T淋巴细胞亚群的检测

目的：通过对尖锐湿疣（CA）患者皮损及外周血T淋巴细胞亚群检测的分析，探讨患者系统和局部的细胞免疫功能变化及其对CA的影响。方法：通过流式细胞仪对40例CA患者及20名正常人外周血进行T淋巴细胞亚群的检测，采用免疫组化的方法对40例CA皮损中CD4^＋和CD8^＋细胞的分布情况进行检测。结果：CA患者外周血及皮损中CD8^＋细胞百分率增加，CD4^＋细胞百分率及CD4^＋／CD8^＋比值降低，且皮损中的尤为明显。结论：CA患者存在全身和局部的细胞免疫功能低下，而且局部细胞免疫功能低下可能在CA的发生和发展中具有重要的作用。     

扁平苔藓患者外周血T淋巴细胞亚群的检测

应用流式细胞术对29例扁平苔藓患者外周血T淋巴细胞亚群CD4^＋、CD8^＋及CD25^＋进行检测分析。结果：扁平苔藓患者外周血T淋巴细胞亚群CD8^＋明显升高，CD4^＋／CD8^＋、CD25^＋均明显降低，与健康对照组比较有显著性差异（P〈0．05）。提示扁平苔藓患者存在细胞免疫功能紊乱。     

特应性皮炎患者外周血表达不同细胞因子T细胞亚群的检测

目的 探讨特应性皮炎患者外周血表达不同细胞因子T细胞群的频数及其与疾病严重程度的相关性。方法 采用流式细胞仪，检测外周血表达不同胞质内细胞因子（包括IL-4、IL-5、IFN-γ、TNF-α）的CD4+ T、CD8+ T、CLA+ T细胞亚群的频数。利用特应性皮炎皮损面积严重度指数积分法方法，获取特应性皮炎患者病情严重程度积分，分析其与表达不同细胞因子的T细胞频数的相关性。结果 特应性皮炎患者外周血中，胞质内表达IL-4和IL-5的CD4+ T、CD8+ T、CLA+ T细胞频数均高于对照组，胞质内表达IFN-γ的CD4+ T、CLA+ T细胞频数低于对照组；差异均有统计学意义。患者组与正常人对照组中，TNF-α在不同T细胞亚群中表达差异无统计学意义。疾病严重程度与IL-4+CD4+ T细胞和IL-5+CLA+ T细胞群的表达频数相关。结论 患者外周血T细胞存在免疫偏移，表达某些细胞因子的T细胞亚群水平可能作为检测疾病严重程度的指标。     

T细胞、角蛋白10、Ki-67在进展期银屑病皮损和皮损边缘皮肤的表达和意义

目的：探讨T细胞、增殖标记（Ki-67）、角蛋白10阳性细胞在进展期银屑病皮损和皮损边缘皮肤的水平。方法：采用免疫组化法检测19例寻常型银屑病进展期皮损中央、皮损边缘、皮损边缘正常皮肤和远离皮损5em正常皮肤的T细胞亚群、Ki-67和K10^＋的表达。结果：皮损边缘CD4^＋、CD25^＋T细胞数目明显增多，表皮增殖标记（Ki-67）明显增加，而K10^＋呈低表达。结论：CD4^＋、CD25^＋T细胞在银屑病发病的早期起着一定作用，Ki-67表达水平反映了角质形成细胞的增殖状态，K10^＋在银屑病皮损的低表达意味着角化过程普遍受损。     

银屑病患者皮损和外周血中CD8α+α+ T细胞的研究

目的 观察银屑病患者皮损和外周血中CD8α+α+ T细胞的分布和比例,探讨其在银屑病发病中的作用。 方法 采用免疫荧光技术观察5例进展期寻常性银屑病患者和5例健康人皮肤中CD8α+α+ T细胞的分布,流式细胞仪分别检测10例进展期寻常性银屑病患者和8例健康人外周血中CD8α+α+ T细胞比例及干扰素γ（IFN-γ）和肿瘤坏死因子α（TNF-α）的表达。采用Graphpad Prism统计软件对实验组和对照组各数据进行t检验。 结果 在5例银屑病患者皮损真皮浅层均可见以CD8α+α+ T细胞为主的浸润,未见明显的CD8α+β+ T细胞浸润;而5例健康人皮肤中均未见CD8α+α+ T细胞和CD8α+β+ T细胞浸润。流式细胞仪检测,8例健康人外周血中CD8α+α+ T细胞比例为9.12% ± 4.80%,10例银屑病患者组为26.47% ± 12.99%,银屑病患者组明显高于对照组,两组比较,t = 3.96,P ＜ 0.001。在银屑病患者外周血CD8α+α+ T细胞中分泌IFN-γ和TNF-α的细胞比例分别为47.36% ± 19.38%和54.14% ± 21.14%,健康对照组分别为13.44% ± 9.21%和34.03% ± 17.22%,两组差异均有统计学意义（t值分别为4.54和2.17,P值分别 ＜ 0.001和 ＜ 0.05）。 结论 银屑病患者皮损和外周血中存在CD8α+α+ T细胞的分布和比例增多,CD8α+α+ T细胞可能是参与银屑病发病的主要CD8+ T细胞亚群。     

寻常型银屑病患者外周血CD4~+CD25~+及CD8~+CD25~+细胞的检测

目的研究进展期寻常型银屑病患者外周血CD4+CD25+和CD8+CD25+调节性T细胞的数量变化及其在银屑病免疫病理学发病机制中的作用。方法应用流式细胞术对进展期寻常型银屑病患者外周血CD4+CD25+和CD8+CD25+调节性T细胞进行检测。结果进展期寻常型银屑病外周血CD4+CD25+细胞及CD8+CD25+调节性T细胞数量与正常对照组相比,均显著降低(P<0.05,P<0.005),而CD4+CD25+/CD8+CD25+比值无显著性差异(P>0.05)。结论寻常型银屑病的发病与CD4+CD25+和CD8+CD25+调节性T细胞的同步降低有关,与二者的比值无关。     

Circulating CLA+ T cell subsets inversely correlate with disease severity and extension in acute psoriasis but not in chronic plaque psoriasis

Circulating CLA+ T cells represent a subset of lymphocytes functionally associated to several cutaneous diseases. This population of peripheral lymphocytes is poorly characterized in acute stage psoriasis. We studied, by flow cytometry, the relationship between disease severity and extension and different subsets of circulating T cells in 31 psoriatic patients (7 guttate, 8 acute and 16 chronic psoriasis). An inverse correlation between circulating CLA+ CD3+/CD4+ subsets and disease severity and extension was found in the acute form of psoriasis. Interestingly, we also observed that circulating CLA+CD4+CD25+ cells inversely correlated with PASI and BSA in guttate patients, which had not been shown previously. These results may contribute to clarify the role of circulating T cells in psoriasis, especially in early stages of psoriasis.     

寻常型银屑病皮肤中皮肤归巢T细胞免疫组化研究

目的 探讨皮肤归巢T细胞在寻常型银屑病(PV)发病中的作用。方法 采用间接免疫荧光双标法研究正常人皮肤和PV患者皮肤中浸润的皮肤归巢T细胞分类及其变化。结果 ①正常人皮肤及PV皮损中浸润的T细胞绝大多数表达皮肤淋巴细胞相关抗原(CLA),CLA⁺细胞高度表达CD45RO,只有个别为CD45RO阴性。②进行期皮损CD4⁺CLA⁺及CD8⁺CLA⁺T细胞数高于静止期皮损(P<0.05),静止期皮损CD4⁺CLA⁺细胞数高于消退期皮损(P<0.05),消退期皮损CD8+CLA+细胞数高于PV外观正常皮肤(P<0.05),进行期皮损周边外观正常皮肤CD4⁺CLA⁺及CD8⁺CLA⁺细胞数高于静止期皮损周边外观正常皮肤(P<0.05).③部分病例皮损的表皮中见大量CLA⁺树突状细胞,正常人皮肤未见此细胞。结论 正常人皮肤及PV皮损中T细胞绝大多数为皮肤归巢T细胞;进行期及静止期PV皮损中浸润的细胞主要为CD3⁺、CD4⁺、CD45RO⁺、CLA⁺T细胞,CD3⁺、CD4⁺、CD45RO⁺、CLA⁺T细胞可能在PV发病中起重要作用。     

CD8+ cell changes in psoriasis associated with roxithromycin-induced clinical improvement

We have shown that T cell receptor BV2- and BV8-bearing CD8+ cells are decreased in the peripheral blood of psoriatic patients, while T cells possessing these BVs accumulate in psoriatic lesions. T cells homing to skin express cutaneous lymphocyte-associated antigen (CLA), and bacterial superantigens that trigger psoriasis promote this expression. Roxithromycin has immunomodulatory potency and its effectiveness for psoriasis has been proposed. Therefore, we monitored BV usage and alteration of superantigen-promoted CLA expression in circulating CD8+ cells of psoriatics before and after roxithromycin therapy. After roxithromycin treatment, circulating BV2- and BV8-bearing CD8+ cells were increased and CD8+ cells exhibited reduced expression of CLA when stimulated in vitro with bacterial superantigens. It is suggested that roxithromycin downregulates augmented expression of CLA by CD8+ cells, thereby suppressing their skin-homing and elevating the numbers of circulating BV2- and BV8-positive CD8+ cells. Such events may be included in part in the improvement of psoriasis with roxithromycin.     

血热型/血燥型银屑病患者外周血T淋巴细胞亚群变化

目的 探讨血热型/血燥型银屑病患者外周血T淋巴细胞亚群变化规律,并和正常人作比较.方法 选择血热型银屑病患者35例、血燥型银屑病患者30例和正常人36例的外周血单一核细胞,采用流式细胞分析方法检测T淋巴细胞亚群中CD4⁺和CD8⁺的比例.结果 在银屑病患者外周血中,血热CD4M⁺淋巴细胞明显低于血燥型或正常人,CD8⁺淋巴细胞明显高于血燥型或正常人,CD4⁺/CD8⁺比值明显倒置,而血燥型CD4⁺和CD8⁺淋巴细胞与正常人相比没有差异.结论 T淋巴细胞变化可能是血热型/血燥型银屑病辨证求因的主要效应细胞之一.     

Homing receptor and chemokine receptor on intraepidermal T cells in psoriasis vulgaris

Intraepidermal T lymphocytes found in psoriatic skin lesions are involved in the development and maintenance of lesional pathology. It has become clear that differential expression of homing and chemokine receptors determines the specific migration of T cells to distinct tissues and microenvironments, including psoriasis lesions. The aim of the present study was to clarify expression of homing (CLA, VLA-4, and LFA-1) and chemokine (CCR4, CCR6, CCR7, and CXCR3) receptors on intraepidermal T cells in psoriatic lesions using flow cytometry. The vast majority of intraepidermal T cells in psoriatic lesions expressed CLA and LFA-1, whereas 58% of CD4+ and 85% of CD8+ T cells expressed VLA-4. The majority of CD4+ T cells and about half of the CD8+ T cells expressed CCR4 and CCR6, whereas less than one-third of CD4+ and CD8+ T cells expressed CXCR3 or CCR7. In patients with psoriasis the percentages of T cells expressing CLA, CCR4, and CCR6 were much higher in the epidermis of psoriatic plaques than in the peripheral blood. Thus, CLA, CCR4, and CCR6 may play a more important role in the migration of T cells to psoriatic epidermis.     

活化抗原CD69、HLA-DR在寻常性银屑病外周血单一核细胞和皮损中的表达

目的 探讨活化抗原CD69和HLA-DR在寻常性银屑病患者外周血CD3+T淋巴细胞和皮损中的表达变化。 方法 流式细胞仪检测20例寻常性银屑病患者和20例健康对照外周血CD3+T细胞中CD69和HLA-DR的表达,同时采用免疫组化的方法检测15例寻常性银屑病患者和10例健康对照皮损组织中HLA-DR的表达并进行对比。两组统计学分析采用双侧t检验。 结果 CD69和HLA-DR在寻常性银屑病患者外周血CD3+T细胞中的表达率分别为（4.70 ± 1.90）%、（8.97 ± 1.79）%,比健康对照组［（1.56 ± 0.95）%、（3.02 ± 1.15）%］明显升高（均P < 0.01）。Pearson相关性分析发现,CD3+HLA-DR+细胞百分比与银屑病皮损和严重程度评分指数（PASI）评分呈正相关（r = 0.5626,P < 0.05）。与健康对照组比较,寻常性银屑病患者皮损真皮中HLA-DR表达升高,但表皮中HLA-DR表达降低（均P < 0.05）,HLA-DR在健康人主要分布在血管周围,而在寻常性银屑病患者则广泛表达于真皮组织。 结论 寻常性银屑病患者外周血CD3+T淋巴细胞及皮损中的炎症细胞存在异常活化,外周血CD3+HLA-DR+细胞与疾病严重程度存在相关性。     

Increased expression of the natural killer cell inhibitory receptor CD94/NKG2A and CD158b on circulating and lesional T cells in patients with chronic plaque psoriasis

BACKGROUND: Psoriasis is a common inflammatory cutaneous disorder characterized by activated T-cell infiltration. T lymphocytes bearing natural killer cell receptors (NKRs) have been suggested to play an important role in the pathogenesis of psoriasis. However, the expression pattern of activating and inhibitory NKRs on T lymphocytes from psoriatic patients and its significance in psoriasis needs further study. OBJECTIVES: To investigate the pathogenesis of NKR-expressing T cells in psoriasis. MATERIALS AND METHODS: Thirty patients with chronic plaque psoriasis and 20 healthy controls were enrolled in this study. The immunophenotypic profiles of NKRs, including CD56, CD16 (activating NKRs), CD158a, CD158b, CD94 and NKG2A (inhibitory NKRs), were analysed in peripheral blood T lymphocytes, as well as psoriatic lesional infiltrating T cells, by triple-fluorescence flow cytometry. RESULTS: A significant increase of inhibitory CD8+ CD158b+, CD4 CD8 CD158b+ and CD8+ CD94/NKG2A+ T cells was found in the peripheral blood of patients with psoriasis when compared with controls. Tissue-infiltrating T lymphocytes expressing inhibitory receptors CD158b, CD94 and NKG2A were found in psoriatic lesions. There was a significant positive correlation between the increased percentage of circulating CD8+ CD94/NKG2A+ T cells and the Psoriasis Area and Severity Index. CONCLUSIONS: In the present study, we demonstrated increased proportions of particular subsets of inhibitory CD158b+ and/or CD94/NKG2A+ T cells in patients with psoriasis. The elevation of these inhibitory NKR-expressing T cells was correlated with disease severity, which may signify the possibility of chronic antigen-driven stimulation and dysregulated cytokine production in the pathogenesis of psoriasis.     

银屑病皮损CD8αα+ T细胞表型鉴定

目的 探究银屑病皮损局部浸润的CD8+ T细胞表型及其在银屑病发病中的作用。方法 收集2017年1 - 12月第四军医大学西京皮肤医院明确诊断的8例进行期斑块状银屑病患者皮损,男女各4例,年龄24 ~ 50岁。8例健康对照皮肤来源于整形外科手术剩余皮肤,男女各4例,年龄23 ~ 46岁。采用免疫荧光技术检测皮损CD8+ T细胞的分布及亚群比例,鉴定其免疫学表型及炎症因子白细胞介素（IL）17A的表达。结果 8例银屑病皮损真、表皮均可见CD8+ T细胞浸润,其中CD8αα+ T细胞表型占88.48% ± 7.39%,8例健康人皮肤局部仅有个别CD8+ T细胞浸润,其中CD8αα+ T细胞表型占14.43% ± 13.14%,两组比较,t = 11.5,P < 0.01。银屑病皮损表皮CD8αα+ T细胞表达组织局部定植标志CD103,真皮CD8αα+ T细胞不表达CD103。银屑病皮损CD8αα+ T细胞表达CD45RA-CCR7-效应记忆性T细胞表型,不表达CD8+ 调节性T细胞标志Foxp3、CD25和CD122。银屑病皮损CD8αα+ T细胞中分泌IL?17A的细胞比例为24.85% ± 4.25%,对照组CD8αα+ T细胞不分泌IL?17A,两组差异有统计学意义（t = 5.853,P < 0.01）。结论 银屑病皮损浸润的CD8αα+ T细胞是效应记忆性T细胞,可能通过分泌IL?17A参与银屑病的发生发展。