１０２３中药合剂健身防癌作用的动物实验研究

目的：研究１０２３合剂健身防癌的作用，方法：在用ＤＭＢＡ制造仓鼠平面颊癌模型的同时，用１０２３合剂灌胃以观察１０２３用药的健身防癌作用，结果：１０２３用药组在实验后２周动物体重下降少，增重幅度较大，死亡率低，而且肿瘤发生时间比未用１０２３合剂的动物组推迟了１２天，发生率明显低于对照组（Ｐ＜０．０１），结论：１０２３合剂有健身防癌作用。     

口腔癌肿瘤浸润淋巴细胞对人舌癌裸鼠移植瘤的生长抑制作用

目的探讨在体外具有强大细胞毒活性的口腔癌浸润淋巴细胞在体内的抑瘤效果以及化疗药物环磷酰胺（ｃｙｃｌｏｐｈｏｓｐｈａｍｉｄｅ，Ｃｙ）与肿瘤浸润淋巴细胞（ｔｕｍｏｒｉｎｆｉｌｔｒａｔｉｏｎｌｙｍｐｈｏｃｙｔｅ，ＴＩＬ）联合应用治疗口腔癌的可能性。方法将舌鳞癌细胞系Ｔｃａ８１１３注入裸鼠背部皮下建立移植瘤模型，取培养４周的ＴＩＬ联合低剂量Ｃｙ局部注射，观察肿瘤体积变化及肿瘤生长情况。结果①ＴＩＬ＋ｒＩＬ２和ＴＩＬ＋ｒＩＬ２＋Ｃｙ组在３周内抑瘤率较高（分别为：８６１％±０４％和９７７％±０６％），３周后抑瘤作用减弱；②ＴＩＬ＋ｒＩＬ２＋Ｃｙ组抑瘤率较ＴＩＬ＋ｒＩＬ２组高（第８周抑瘤率分别为：２００％±１４％和７５５％±２５％，Ｐ＜００１）。结论口腔癌ＴＩＬ在应用后３周内具较强抑瘤作用，３周后减弱；联合应用Ｃｙ可获得更佳抑瘤效果     

实验性动物癌前损害和癌的凝集素受体变化研究

本文用６种生物素化的凝集素对仓鼠颊囊正常上皮和实验性癌前损害和癌进行了组织化学研究．其结果表明：正常的仓鼠颊囊上皮有５种凝集素染色阳性，但在上皮各层的染色不同；对５种病变组织的染色发现，过度角化的凝集素染色同正常组织．不典型增生的凝集素染色有明显差异，尤其是重度不典型增生的染色改变，在多数情况下同癌的改变．ＷＧＡ的染色在正常仓鼠颊囊上皮与正常人的口腔上皮相似，ＷＧＡ在不同病变组织中呈现不同的染色图像，说明ＷＧＡ可作为口腔癌前损害监测和癌的恶性程度分级指标：ＲＣＡ、ＵＥＡ、ＤＢＡ和ＢＳＬ在不同病变组织中的动态变化均说明，凝集素受体的异常表达是组织不良增生的一个指征；凝集素受体的消失是癌变的一个信号．     

抑癌基因CDKN2与口腔粘膜癌前损害及鳞状细胞癌的相互关系

ＣＤＫＮ２是一种新近发现的抑癌基因，现已查明，野生型ＣＤＫＮ２基因是正常细胞周期中的负性调节因子，而该基因的突变与缺失被认为与多种肿瘤的发生关系密切。并已发现，在口腔粘癌前损害及鳞状细胞癌中有该基因的改变及表达异常，为口腔粘膜鳞状细胞癌的发生机理研究开辟了新的领域。本文对ＣＤＫＮ２基因的一般性质、作用机理及其与口腔粘膜癌前损害，鳞状细胞癌关系的研究现状作一综述。     

仓鼠口腔癌变过程中表皮细胞生长因子受体mRNA的表达

仓鼠口腔癌变过程与人头颈部鳞状细胞癌形成过程相似，为了解表皮细胞生长因子受体（ｅｐｉｄｅｒｍａｌｇｒｏｗｔｈｆａｃｔｏｒ，ｒｅｃｅｐｔｏｒ，ＥＧＦＲ）与头颈部鳞癌形成之间的关系，采用原位逆转录多聚酶链反应（ＲＴＰＣＲ）方法对仓鼠口腔癌变过程中ＥＧＦ...     

实验性动物癌前损害及癌凝集素受体变化的研究

实验性动物癌前损害及癌凝集素受体变化的研究陈作良陈新民官玉芹周新华陈汉正在对口腔粘膜癌前损害及癌的上皮抗原性改变的研究中，凝集素受体变化的研究正日益受到重视。我们对ＤＭＢＡ制造的仓鼠颊囊癌前损害和癌的凝集素受体变化进行系列观察，以期发现凝集素受体表达...     

口腔鳞状细胞癌与人乳头状瘤病毒感染的关系

本文用抗牛乳头状瘤病毒（ＢＰＶ）的抗血清对３１例口腔鳞状细胞癌中的人乳头状瘤病毒（ＨＰＶ）结构抗原进行检测，证明其中３０例口腔鳞状细胞癌中ＨＰＶ结构抗原一，说明口腔癌的发病与ＨＰＶ感染有密切关系，对防治ＨＰＶ感染，减少口腔癌的发病有着重要意义。     

放射联合淋巴细胞对腺样囊性癌的抗瘤效应

目的 探索放射联合聩瘤引流区淋巴结淋巴细胞（ｔｕｍｏｒ ｄｒａｉｎｉｎｇ ｌｙｍｐｈ ｎｏｄｅ ｌｙｍｐｈｏｃｙｔｅ,ＤＮＬ对肿瘤细胞的作用。方法 取首次治疗病理证实的口腔癌患者颈清扫淋巴结,经常规消化、分离,加白细胞介素２（ｉｎｔｅｒｌｅｕｋｉｎ－２,ＩＬ－２）培养,获取激活的淋巴细胞作为效应细胞,以效靶比（Ｅ：Ｔ）２５：１进行集落形成试验,观察对人涎腺腺样囊性癌肺高转移细胞析（ａｄｅｎｏｉｄ     

人乳头瘤病毒16型在口腔癌及癌旁组织中的存在状态

采用斑点杂交和Ｓｏｕｔｈｅｒｎ印迹杂交检测２５例口腔鳞癌及其相应癌旁组织中的人乳头瘤病毒（ＨＰＶ）１６型ＤＮＡ，口腔癌ＨＰＶ１６ＤＮＡ的阳性率为２４．０％（６／２５），癌旁组织为８．０％（２／２５）。经ＢａｍＨＩ、ＰｓｔＩ酶切杂交后出现的阳性区带显示ＨＰＶ１６ＤＮＡ在口腔癌及癌旁组织中均以游离状态存在。作者推测ＨＰＶ１６ＤＮＡ在口腔癌的致癌机理中可能作为一个协作因子发生作用。     

维拉帕米增强米托蒽醌对腺样囊性癌抑癌效应的实验研究

本研究首次在体内外观察了钙拮抗剂维拉帕米（ＶＰ）与抗癌新药米托蒽醌（ＤＨＡＤ）合用，对人腺样囊性癌ＡＣＣ－２细胞的作用。发现：无毒剂量的ＶＰ可显著增强ＤＨＡＤ对ＡＣＣ－２细胞增殖的抑制，两药合用对ＡＣＣ－０２细胞的细胞毒有协同效应。￣３Ｈ－ＴｄＲ掺入试验表明，ＶＰ可增强ＤＨＡＤ对ＡＣＣ－２细胞ＤＮＡ合成的抑制作用。两药合用后，ＤＨＡＤ对ＡＣＣ－２细胞ＤＮＡ合成的ＩＣ＿（５０）值下降３．３２倍。流式细胞仪分析显示，ＤＨＡＤ佐以ＶＰ明显增强对ＡＣＣ－２细胞Ｇ＿２Ｍ期的阻断作用。裸鼠体内抑瘤实验证实，ＶＰ（５０ｍｇ／ｋｇ）明显增强ＤＨＡＤ（２ｍｇ／ｋｇ）对ＡＣＣ－２种植瘤生长的抑制作用，抑瘤率由单用ＤＨＡＤ的５７．９％提高到８８．４％。结果提示，可以将ＶＰ作为ＤＨＡＤ的增效剂用于涎腺癌临床化疗，以提高治疗效果。     

1023中药合剂对口腔癌前病变的治疗和阻癌作用的临床研究

目的：探讨1023对口腔白班（OLK）、口腔扁平苔藓（OLP）和盘状红斑狼疮（DLE）等癌前病变的治疗作用和阻断癌变的作用。方法：将1023合剂用于48例OLK、139例OLP和38例DLE，并设对照组，实验组和对照组均接受相同的常规治疗。结果：1023对OLK和OLP治疗有明显的效果，其有效率明显高于对照组（P＜0．05），而OLK的癌变率明显低于对照组（P＜0．05）。结论：1023合剂对OLK和OKP均有治疗作用，对OLK有阻止癌变的作用。     

对乙酰氨基酚及肿痛安对大鼠正畸牙移动的比较研究

目的通过比较对乙酰氨基酚(扑热息痛)和肿痛安对大鼠正畸牙移动过程中组织学差异,为正畸临床疼痛控制提供参考。方法45只6～8周龄Wistar雄性大鼠随机分为生理盐水组、肿痛安组、扑热息痛组,每组15只。通过切牙和磨牙区安装弹簧,建立正畸牙移动模型。运用HE染色、TRAP染色及免疫组化方法观测磨牙破骨细胞数量及COX-2的变化,单因素方差分析。结果实验组和对照组在正畸牙移动过程中牙槽骨内破骨细胞量及COX-2变化量并无显著性差异,实验组之间亦无显著性差异。结论扑热息痛和肿痛安在40g力加载时均不影响正畸牙移动,但是否可用于正畸临床有效控制疼痛尚待于进一步研究。     

中西医结合治疗贝尔氏面瘫

目的　评定中西医结合治疗Bell′s面瘫的临床疗效。方法　83例Bell′s面瘫患者随机分为试验组(54例) 和对照组(29例)。两组患者均采用药物、针刺、理疗进行治疗;试验组在此基础上增加按摩与功能训练;两组疗效均采用Portmann简易评分法评分,评价疗效。结果　治疗前评分试验组为2·907±1·794,对照组为2·931±2·034,治疗后评分试验组为18·593±1·743,对照组为9·862±3·091。试验组面肌功能评分及面肌功能改善指数明显高于对照组(P<0·01)。结论　中西结合治疗Bell′s面瘫有明显的康复疗效;面肌功能评定能客观地反应患者治疗前后面肌运动功能改善情况,量化地评价Bell′s面瘫患者的治疗效果。     

A randomized clinical trial for the evaluation of head and neck squamous cell carcinoma treating with traditional Chinese medicine]

This paper described the research design,research process and statistical analysis of randomized clinical trial by treatment with traditional Chinese medicine in patients with squamous cell carcinoma of the head and neck.At first we found there was no significant difference between treating group and contral group(P>0.05) using log-rank test on Kaplan-Meier survival curves. With further multivariate analysis Cox model and after adjusting for sex,age,legth of taking the assigned medication,grade of tumor differentiation and using of chemotherapy,the rate of tumor-related death for control group was significantly higher than that for treating group :hazards ratio estimate was 1.939(95% confidence interval 1.008-3.729,(P<0.05).The length of taking Chinese medicine was one of the most important prognostic factors(P<0.001).The results suggest that the prescribed medication contributed to the improved survival,and it was an independent prognostic factor.     

中西医结合治疗儿童复发性口腔溃疡的临床效果分析

目的：评价中西医结合治疗小儿复发性口腔溃疡的临床效果及对患儿免疫功能的影响。方法：76例复发性口腔溃疡的小儿患者,随机分为实验组和对照组各38例。对照组给予西药对症支持治疗,实验组患儿在此基础上联合中药方剂调理。观察两组的溃疡愈合时间,疼痛评分,治疗有效情况及对患儿免疫功能的改善情况与治疗期间的不良反应。结果：实验组治疗后的平均愈合时间明显短于对照组（ t＝6．7145,P＝0．0000）;两组的T细胞亚群含量比较,治疗后均有所改善（P＜0．05）,而实验组的CD4、CD8和CD4／CD8的改善情况明显优于对照组（P＜0．05）;两组患者治疗期间均未发生明显不良反应。结论：采用中西医结合治疗小儿复发性口腔溃疡临床疗效显著,同时可提高患儿的免疫功能。     

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目的评价中西医结合治疗糜烂型口腔扁平苔藓(orallichenplanus,OLP)的临床疗效。方法选择2008年2月至2010年6月在江西省抚州市第一人民医院口腔科就诊的86例糜烂型OLP患者,随机分为试验组和对照组,各43例。试验组采用局部注射曲安奈德配合中药治疗,对照组仅局部注射曲安奈德。分别对两组患者治疗前、治疗1个月后和治疗6个月后的局部病损进行分级评定,并进行对比观察。结果治疗1个月后试验组和对照组有效率分别为83.33%和83.72%,两组比较,差异无统计学意义(χ2=0.002,P=0.962);治疗6个月后试验组有效率为76.68%,优于对照组的26.32%,差异有统计学意义(χ2=17.053,P<0.001)。结论中西医结合治疗糜烂型OLP的疗效优于单纯的局部注射治疗。     

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??Objective    To investigate the influence of Bushen Zhuanggu Decoction on the bone healing after teeth extraction in osteoporotic dogs. Methods    Totally 9 female adult dogs whose bilateral ovaries were surgically removed to establish osteoporosis models were randomly divided into 3 groups ??control group??Western medicine group and traditional Chinese medicine group??. Bilateral mandibular premolars were extracted 3 months after castration. Osteoporosis treatment was started the third day after tooth extraction. The dogs in the traditional Chinese medicine group??the Western medicine group and the control group were respectively fed with Bushen Zhuanggu Decoction??Estradiol Valerate Tablets and nothing. The bone mineral density??BMD?? value of dog′s lumbar spine and mandible of each group were detected before and after 3 months of castration and 3 months after teeth extraction. The tissue morphology of the mandible was observed at 1??2 and 3 months after teeth extraction. Results    Three months after teeth extraction??the BMD values of the lumbar spine and mandible in the traditional Chinese medicine group and the Western medicine group were significantly increased compared with those before the osteoporosis treatment??and there was no significant difference compared with the preoperative ones. One month after teeth extraction?? the new bone and the trabecular bone were observed in irregular patterns in the control group and the Westen medicine group??and those were arranged in a mesh pattern in the Chinese medicine group. Two months after teeh extraction??the trabecular bone structure was sparse in the control group??the Western medicine group was more than that of the new bone??and the number of trabecular bone in the Chinese medicine group was more robust. Three months after teeth extraction??the bone trabecular structure was relatively compact in the control group and the gap is small??the new bone tissue and the original bone tissue roughly fused together in the Western medicine group??the new bone tissue of the traditional Chinese medicine group was more similar to the structure of the original bone. Conclusion      Bushen Zhuanggu Decoction can make cancellous bone around the teeth extraction of the osteoporosis canine and significantly increase the amount of bone tissue??so as to promote bone reconstruction and bone healing after teeth extraction.     

口灵药膜治疗复发性口疮临床疗效分析

目的：探讨口灵药膜治疗复发性口疮（RAU）的临床疗效。方法：治疗组80例RAU患者采用口灵中药药膜治疗,对照组80例RAU患者采用西药常规治疗,观察用药后的平均溃疡期及疼痛指数的变化。结果：治疗组平均溃疡期明显缩短,疼痛指数明显下降。结论：药膜治疗复发性口疮能减轻患者痛苦,使病程缩短,并优于西药治疗组。     

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??Objective    To explore the therapeutic effect of combined therapy of traditional Chinese medicine and western medicine on burning mouth syndrome. Methods    Eighty patients with burning mouth syndrome were randomly divided into observation group and control group??40 cases in each group. The observation group received traditional Chinese medicine and western medicine treatment combined with psychological therapy??while the control group was given traditional Chinese and western medicine treatment??two groups were compared before and after treatment. Results    In observation group 27 cases were markedly effective??11 cases effective??2 cases invalid??total effective rate 95.0%??in control group it was markedly effective in 4 cases??effective 13 cases??invalid 23 cases??total effective rate 42.5%. The clinical effect of the observation group was significantly better than that of the control group??P??0.05??. There were no significant differences in clinical symptoms in two groups before treatment??P??0.05????after treatment??tongue burning pain??numbness??irritability and insomnia symptoms of tongue in the observation group were significantly lower than those in the control group??P??0.05??. There was no significant difference between the two groups of patients before treatment concerning the Hamilton Anxiety Rating and Hamilton Depression??P??0.05????after treatment??Hamilton Anxiety Rating and Hamilton depression of the observation group were significantly lower than the control group??P??0.05??. Conclusion            Combination of traditional Chinese medicine and western medicine therapy along with psychological intervention in the treatment of burning mouth syndrome not only improves the clinical symptoms??but also can improve the quality of life of patients with good clinical efficacy.