Two new and four known polyphenolics obtained as new cell-cycle inhibitors from Rubus aleaefolius poir

Two new polyphenolics, rubuphenol (1) and sanguiin H-2 ethyl ester (2), were isolated together with ellagic acid (3), ethyl gallate (4), 1,2,3,4,6-penta-O-galloyl-g -D-glucopyranose (5) and 1,2,3,6-tetra-O-galloyl-g -D-glucopyranose (6) as new cell-cycle inhibitors from Rubus aleaefolius by bioassay-guided separation procedure and the structures of 1 and 2 were elucidated by spectroscopic method. Compounds 1 - 6 inhibited the cell cycle progression of tsFT210 cells at the G₀/G₁ phase with the MIC values of 14.6 w M (1), 22.1 w M (2), 10.3 w M (3), 7.8 w M (4), 7.9 w M (5) and 6.6 w M (6).     

Carbazole alkaloids as new cell cycle inhibitor and apoptosis inducers from Clausena dunniana Levl

Carbazole alkaloids, 3-methylcarbazole (1), murrayafoline A (2), girinimbine (3), mahanimbine (4) and bicyclomahanimbine (5), were isolated for the first time from Clausena dunniana Levl. by bioassay-guided separation procedure and were identified by spectroscopic methods. Compounds 1 - 5 showed growth inhibitory activity (1, IC₅₀ 25 μg/ml) on human fibrosarcoma HT-1080 cells and cell cycle M-phase inhibitory (2, MIC 0.78 μg/ml) and apoptosis inducing (2, MIC 1.56 μg/ml; 3, MIC 25 μg/ml; 4, MIC 20 μg/ml; 5, MIC 30 μg/ml) activities on mouse tsFT210 cells, respectively, and 2 - 5 provided the first examples of carbazole alkaloids as new cell cycle inhibitors and apoptosis inducers.     

Carbazole alkaloids as new cell cycle inhibitor and apopTosis inducers from Clausena dunniana Levl

Carbazole alkaloids, 3-methylcarbazole (1), murrayafoline A (2), girinimbine (3), mahanimbine (4) and bicyclomahanimbine (5), were isolated for the first time from Clausena dunniana Levl. by bioassay-guided separation procedure and were identified by spectroscopic methods. Compounds 1 - 5 showed growth inhibitory activity (1, IC₅₀ 25 w g/ml) on human fibrosarcoma HT-1080 cells and cell cycle M-phase inhibitory (2, MIC 0.78 w g/ml) and apoptosis inducing (2, MIC 1.56 w g/ml; 3, MIC 25 w g/ml; 4, MIC 20 w g/ml; 5, MIC 30 w g/ml) activities on mouse tsFT210 cells, respectively, and 2 - 5 provided the first examples of carbazole alkaloids as new cell cycle inhibitors and apoptosis inducers.