HPLC法测定滴鼻灵中地塞米松磷酸钠的含量

目的：建立HPLC法测定滴鼻灵中地塞米松磷酸钠的含量,方法：以十八烷基硅烷键合硅胶为填充剂,0．01mol.L^-1磷酸二氢钾（KH2PO4）－甲醇－磷酸（48：52：0．4）为流动相,检测波长240nm。结果：地塞米松磷酸钠在1．26－10．08ug/ml^-1(r=0.9999)范围内线性关系良好。平均回收率为99．16％,RSD为0．65％（n=6)。结论：本方法快速,准确,可用于测定滴鼻灵中地塞米松磷酸钠的含量。     

黑果小檗中小檗碱的含量测定

目的：建立黑果小檗中小檗碱的含量测定方法。方法：CH3OH提取，提取液经处理后．用容量法进行含量测定。结果：提取液经K2Cr2O7处理后24h．稳定性能良好，RSD=0．308％(n=4)；回收率为102．63％，RSD=2．96％(n=4)。结论：本法可用于黑果小檗的质量检测。     

HPLC法测定急麻颗粒剂中黄芩苷的含量

目的：本应用HPHC法浊定麻颗粒剂中黄芩苷的含量,方法：色谱柱ODS,流动相为甲醇－磷酸液（0．4％）（60：40）,检测波长为280nm,结果：含量测定结果为1.551mg.g^-1(n=3),线性关系范围,黄芩苷在0.5-4ug范围内线关系良好（r=0.9993),平均回收率为98．43％,RSD为1．11％（n=6),结论：该法操作简便,快速,可用于该药中黄芩苷的含量测定。     

紫外双波长及二阶导数法测定复方氯苯那敏滴鼻剂中两组分含量

目的：建立复方氯苯那敏滴鼻剂的含量测定方法。方法：采用紫外双波长及二阶导数法分别测定制剂中马来酸氯苯那敏和盐酸麻黄碱的含量,结果：方法的线性关系良好,氯苯那敏的回收率平均值为98．58％,RSD＝0．895（n=6),麻黄碱的顺收率平均值为99．65％,RSD＝1．35％（n=6),精密度测定,氯苯那敏的日内精密度RSD＜0．70％,日间精密度＜0．90％,麻内碱日内RSD＜1．10％,日间RSD＜1．20％,样品测定重视性好,结论：本法准确,精密,简单,快速。     

高效液相色谱法测定玉液消渴颗粒中葛根素的含量

目的建立高效液相色谱法测定玉液消渴颗粒中葛根素含量的方法。方法采用C18色谱柱（250mm×4.6mm,5μm）;甲醇-水（25：75）为流动相;流速1．0mL／min;检测波长250nm。结果葛素根在0．066-0.66μg（r=0．9997,n=6）范围内具有良好的线性关系,平均回收率97．30％,RSD=1．57％（n=6）。结论该法简单、准确、可作为玉液消渴颗粒的含量测定方法。     

HPLC法测定畅中胶囊中厚朴酚与和厚朴酚的含量

目的建立用反相高效液相色谱法同时测定畅中胶囊中厚朴酚与和厚朴酚含量的方法。方法以乙睛-水-冰醋酸（60：38：2）为流动相,SHIMADZU VP-ODS（250mm×4．6mm,5μm）为固定相;检测波长为294nm;流速：1．0ml／min。结果厚朴酚在0．2008～5．02μg的浓度范围内线性关系良好,r=0．9999（n=5）,平均回收率为99．5％,RSD为0．5％;和厚朴酚在0．1202～3．005μg的浓度范围内线性关系良好,r=0．9999（n=5）,平均回收率为99．4％,RSD为0．5％。结论该法结果准确、方法简便、分离度高可作为该制剂质量标准的内容。     

HPLC法测定甲硝唑药膜的含量

目的建立HPLC法测定甲硝唑药膜中甲硝唑的含量。方法以十八烷基硅烷键合硅胶为填充剂,甲醇-水（20：80）为流动相,检测波长为320nm;流速：1．0ml·min-1;柱温：30℃。结果甲硝唑在0．04998—0．59976mg·ml-1的范围内线性关系良好（r=0．9998）,平均回收率为100．02％,RSD为0．50％（n=9）。结论本方法简便、测定结果准确,可用于甲硝唑药膜中甲硝唑的含量测定。     

RP-HPLC法测定止咳橘红口服液中柚皮苷的含量

目的 建立RP-HPLC法测定止咳橘红口服液中有效成分柚皮苷含量的方法。方法 采用HPLC法，色谱柱为C18柱，流动相为甲醇-冰乙酸-水（70：3：130），检测波长为283nm。结果 柚皮苷线性范围13．0～130μg。ml^-1，r=0．999 9（n=5）；止咳橘红口服液中柚皮苷回收率为99．0％（n=9），RSD为1．06％。结论 测定方法简便、稳定，能有效地控制该制剂的质量。     

复方硫唑乳膏的质量控制

目的制备复方硫唑乳膏剂并进行质量控制。方法取处方量油相与水相分别置玻璃烧杯中,置水浴加热75℃使其熔化后,在相同温度条件下,两液合并搅拌至凝（约50％）时加入升华硫、甲硝唑及醋酸氯已定即得乳膏剂。采用碘量法用碘滴定液（0．05mol·L^-1）测定硫的含量。结果该制剂稳定,硫含量在3％～8％浓度范围内线性关系良好（r=0．9998）。加样回收率试验,平均回收率为99．52％,RSD为0．20％（n=9）。结论本制剂处方工艺设计合理,质量控制方法简便易行。     

连梅饮中黄芩苷和小檗碱的含量测定

目的探讨连梅饮中黄芩苷和小檗碱的含量测定方法。方法采用RP—HPLC／UV法测定连梅饮中黄芩苷和小檗碱的含量;色谱柱：Agilent HC C18柱（250×4．6mm,5μm）;乙腈：0．05mol／L磷酸二氢钾溶液梯度洗脱;检测波长275nm;流速1ml／min。结果黄芩苷在0．448—112μg／ml的浓度范围,小檗碱在0．424～106μg／ml的浓度范围内线性良好：低和高浓度的黄芩苷和小檗碱的精密度（RSD）分别为3．88％、4．84％、4．63％、2．29％;加样回收率分别为（104．3±1.3）％、（97．7±6．9）％;连梅饮中黄芩苷的含量为（2．67±0．012）mg／ml,RSD为0．45％（n=3）;小檗碱的含量为（2．47±0．014）mg／ml,RSD为2．98％（n=3）。结论本方法快速、简便、准确,可用于测定制剂中黄芩苷和小檗碱的含量。     

氯霉素羊毛脂软膏含量测定方法研究

目的 建立氯霉素羊毛脂软膏的测定方法。方法10％氯霉素羊毛脂软膏经石油醚溶解、温水提取后,用紫外分光光度法测定氯霉素含量。结果氯霉素的平均回收率为99．84％,RSD为0．25％(n=9)。结论 本方法简便,准确。可用于测定氯霉素羊毛脂软膏的含量。     

The effect of meal energy content on gastric emptying

Gastric emptying (GE) has been reported to be influenced by meal weight (grams) and composition (% carbohydrate:protein:fat) but the effect of meal energy content (kcal) on gastric emptying, independent of composition, has not been thoroughly investigated by scintigraphic techniques. In order to determine the effect of caloric content on GE, 12 normal male volunteers, ranging in age from 19-28 yr (mean = 24 yr), had GE scans (n = 32) performed with a solid meal of fixed composition (carbohydrate 40%:protein 40%:fat 20%) but varying energy content [150 kcal (n = 9), 300 kcal (n = 14), and 600 kcal (n = 9)]. Increasing the energy content of the meal of fixed composition progressively delayed gastric emptying: T1/2 = 57', 70', and 95' for 150 kcal, 300 kcal and 600 kcal, respectively (p less than 0.05; Student's t-test). Reproducibility, evaluated by performing GE scans (n = 8) on different days in the same individual (n = 4) with the same meal (300 kcal), was good (r greater than or equal to 0.89). Variability varied inversely with meal energy content (CV:150 kcal greater than or equal to 300 kcal and 600 kcal). The 300 kcal mean seems to be the best compromise since it results in less intersubject variability than 150 kcal, has minimal intrasubject variability, but is not as prohibitively large as the 600 kcal meal.     

养蔚灵颗粒的制备和质量控制

目的 建立养蔚灵颗粒质量控制方法.方法 处方中的党参、甘草和芍药采用薄层色谱法进行鉴别,并用高效液相色谱法测定处方中芍药苷的含量.结果 定性鉴别方法分离度良好,专属性强;芍药苷含量在10.00～50.00 μg/ml范围内线性关系良好(r=0.9997,n=5),平均回收率(99.63±0.70)％(n=9).结论 养蔚灵颗粒的定性、定量方法快速、准确、特异性强,可作为养蔚灵颗粒的质量控制方法.     

高效液相色谱法测定氯呋滴耳液的含量

目的建立氯呋滴耳液含量测定的高效液相色谱法。方法色谱柱为Shim-pack VP-ODS C18;流动相为甲醇-水-三乙胺-冰醋酸（50∶50∶0.2∶1）;检测波长为240nm;柱温为室温;流速为1ml/min。结果呋喃西林在0.004 052-0.08104mg.ml^-1（r=0.999 9,n=6）,氯霉素在0.124 74-2.494 76mg.ml^-1（r=0.999 9,n=6）范围内呈良好线性关系;平均回收率分别为99.61%,98.82%;RSD分别为0.47%,0.98%（n=9）。结论本法检测快速,定量准确,可用于氯呋滴耳液的定量分析。     

重量分析法测定硫酸钡(Ⅰ型)的含量

目的:建立硫酸钡(Ⅰ型)的含量测定方法.方法:重量分析法.结果:硫酸钡(Ⅰ型)在0.4006-0.8002 g(r=0.9999,n=5)范围内呈良好的线性关系,平均回收率为99.50%,RSD为0.41%(n=9).结论:本法可用于硫酸钡(Ⅰ型)的含量测定.     

HPLC法同时测定复方氯已定含漱液中葡萄糖酸氯已定及甲硝唑的含量

目的用HPLC法同时测定复方氯已定含漱液中葡萄糖酸氯已定和甲硝唑的含量。方法采用Agela Technologies C18（250 mm×4.6 mm,5μm）为色谱柱;以乙腈：磷酸-三乙胺缓冲液（pH为3.2,15∶85）为流动相;检测波长为280nm,流速1.0 ml·min-1。结果甲硝唑、葡萄糖酸氯已定回收率分别为97.05%、98.41%,RSD分别为0.59%、1.89%（n=9）。结论本方法快捷、简便、灵敏,可作为该制剂质量控制的有效方法。     

鲨鱼硫酸软骨素两种测定方法的比较研究

目的 比较2种鲨鱼硫酸软骨素含量的测定方法.方法 以硫酸软骨素、氨基己糖为标准品,采用咔唑法、Elson-Morgan法分别测定鲨鱼软骨提取物中硫酸软骨素的含量.结果 咔唑法在4.710～47.100 μg/ml范围内,线性关系良好(r=0.9999),溶液在3 h内稳定性良好,相对标准差RSD=1.21%,重现性试验RSD=1.36%(n=6),精密度试验RSD=0.46%(n=6),平均回收率为99.60%,RSD=1.39%(n=6).Elson-Moran法在2.552～25.520μg/ml范围内,线性关系良好(r=0.9996),溶液在3 h内稳定性良好,RSD=1.62%,重现性试验RSD=2.61%(n=6),精密度试验RSD=1.07%(n=6),平均回收率为96.55%,RSD=2.43%(n=6).结论 咔唑法测定鲨鱼硫酸软骨素含量较Elson-Morgan 法简便、快速、准确,可作为测定鲨鱼硫酸软骨素含量的方法.

Abstract：

Objective To compare the two methods for the determination of the shark chondroitin sulfate. Methods Using chondroitin sulfate and aminohexose as standards to determine the content of the shark chondroitin sulfate by the carbazole method and the Elson-Morgan method. Results Experiments showed that by the carbazole method, the concentration of the shark chondroitin sulfate was within the ranges of 4. 710-47. 100 μg/ml, displaying good linear relationship (r =0.9999) and good stability within 3 hours, with RSD = 1.21%, reproductive test RSD = 1. 36%( n=6), precision test RSD = 0. 46% (n = 6), and a recovery rate of 99.60% , RSD = 1. 39% ( n = 6). For the Elson-Morgan method, the concentration of the shark chondroitin sulfate was within the ranges of 2.552-25. 520 μg/ml, also displaying good linear relationship (r = 0. 9996) and good stability within 3 hours, with RSD = 1. 62% , reproductive test RSD = 2. 61% ( n = 6), precision test RSD = 1.07%(n = 6), and a recovery rate of 96. 55% ,RSD = 2.43% (n = 6). Conclusions When compared with the Elson-Morgan, the carbazole method was more simple, rapid and accurate and could be used a standard method for the determination of the content of shark chondroitin sulfate.     

双波长分光光度法测定乳康口服液中碘化钾的含量

目的建立乳康口服液中碘化钾的含量测定方法。方法采用双波长等吸收分光光度法,在（225±1）nm和（279±1）nm波长处分别测定碘化钾吸光度以供差值计算碘化钾的含量,消除制剂中附加剂的干扰。结果碘化钾在3～9μg/ml浓度范围内呈良好线性关系,r=0.9999;平均回收率为100.1%,RSD为0.42%（n=6）。结论本方法操作简便,结果准确可靠,可用于乳康口服液的含量测定。     

Protein precipitation: an expedient procedure for the routine analysis of the plasma metabolites of [123I]IBZM

Plasma metabolite analysis of the single photon emission computed tomography (SPECT) D2/D3 receptor radiotracer (S)(-)-N-[(1-ethyl-2-pyrrolidinyl)methyl]-2-hydroxy-3-[123I]iodo-6-metho xybenzamide ([123I]IBZM) is needed for the equilibrium analysis of the SPECT data, in brain imaging studies involving bolus plus constant infusion paradigm. The purpose of these experiments was to find an appropriate procedure to expedite this analysis during routine determinations. The procedure was applied to the plasma analysis of 22 human subjects. Each plasma sample was subjected to acetonitrile protein precipitation. After separation of the pellet, the acetonitrile fraction contained 91% +/- 2% (n = 88) of the mixture of labeled metabolites and parent compound. The recovery coefficient of unmetabolized [123I]IBZM determined with an standard plasma sample was 95% +/- 2% (n = 22). The percent parent compound present in the extracted fraction, measured by high performance liquid chromatography, was 16% +/- 9% (n = 85) and the percent metabolites was 84% +/- 9% (n = 85). Free fraction determination (f1, fraction of radiotracer unbound to protein), was 4% +/- 0.8% (n = 22). Free fraction of parent was 15% +/- 8% (n = 85). The results indicate that acetonitrile protein precipitation is an adequate method for the analysis of the [123I]IBZM plasma metabolites.     

香菇多糖中药注射液含量测定方法的改进

目的改进香菇多糖中药注射液的含量测定方法。方法采用蒽酮比色法测定多糖中药的含量,葡聚糖T500为对照品,检测波长:625 nm。结果葡聚糖T500在0.010～0.040 mg/ml范围内呈良好的线性关系(r=0.9985),平均回收率为96.26%,RSD为1.20%(n=9)。结论本方法操作简便、准确、重复性好,可用于香菇多糖中药注射液的质量控制。