Effect of excisional wounding on DMBA -induced hamster tongue carcinogenesis

The purpose of this study was to investigate histologically the effect of wounding on the hamster tongue after pretreatment with DMBA. The animals in which the tips of the tongues were pretreated with DMBA for 8 weeks, subsequently excised, and had no treatment or received applications of acetone, showed epithelial dysplasias. The animals which had the same pretreatment, excision, and received additional post-excision applications of DMBA for 9–13 days, developed squamous cell carcinomas. However, the animals which were pretreated with DMBA for 8 weeks but had no excision, did not show any pathologic changes, even though they received additional applications of DMBA for 9–13 days. The animals which received no pretreatment with DMBA for 8 weeks hut had an excision, showed normal wound healing, even though they had a post-treatment with DMBA for 9-13 days. The results of the present study indicated that excisional wounding acted as a promotional stimulus in inducing the appearance of epithelial dysplasias or carcinomas of tongues initiated with the carcinogen DMBA.     

Effects of isoproterenol on chemical carcinogenesis with DMBA in mouse salivary glands

Abstract. Histopathological studies were conducted on experimental carcinogenesis in mouse salivary glands using D L-isoproterenol hydochloride (isoproterenol) and 9 10-dimethyl-1 2-benzantliracene (DMBA). DM BA was admisistered once at 1 mg/0.02 ml in olive oil to the salivary glands, and isoproterenol was injected intraperitoneally at 1 mg/0.05 ml saline solution three times a week for 2 weeks al different periods during the oncogenic process. Salivary glands treated with carcinogen showed a relatively constant sequence of changes and developed epithelial and mesenchymal tumors irrespective of isoproterenol administration. Isoproterenol did not influence the development of another type of tumor; however, it did retard the oncogenic processes.     

Effects of isoproterenol on chemical carcinogenesis with DMBA in mouse salivary glands.

Histopathological studies were conducted on experimental carcinogenesis in mouse salivary glands using D L-isoproterenol hydochloride (isoproterenol) and 9 10-dimethyl-1 2-benzanthracene (DMBA). DMBA was administered once at 1 mg/0.02 ml in olive oil to the salivary glands, and isoproterenol was injected intraperitoneally at 1 mg/0.05 ml saline solution three times a week for 2 weeks at different periods during the oncogenic process. Salivary glands treated with carcinogen showed a relatively constant sequence of changes and developed epithelial and mesenchymal tumors irrespective of isoproterenol administration. Isoproterenol did not influence the development of another type of tumor; however, it did retard the oncogenic processes.     

二甲基苯并蒽诱导地鼠颊癌过程中Rb基因产物表达的研究

目的探讨实验性吕必生过程中Ｒｂ基因编码产物（ｐＲｂ）的表达变化。方法 用０．５％的二甲基苏醒 并蒽（ｄｉｍｅｔｈｙｌ－ｂｅｎｚａｎｔｈｒａｃｅｎｅ,ＤＭＢＡ）丙酮液涂技法 鼠石侧颊粘膜３次／周,对病变组织用ＬＳＡＢ法进行免疫组化染色（ｐＲｂ染色最强,３周（单纯增生）尖似正常;６、９周异常增生损害时染色减弱,与异常增生程度有关;１２周 形成时染色进一步减弱。ｐＲｂ染色降低与恶变进展有关。结论 ＤＭＢ     

金地鼠颊囊癌变过程中p65蛋白的表达研究

目的探讨核转录因子-κB家族成员中的一个重要亚基p6 5在DMBA诱导的金地鼠颊囊鳞状细胞癌发生过程中的表达及意义。方法建立金地鼠颊囊癌变的动物模型。采用Westernblot法检测12组配对金地鼠正常颊粘膜、上皮单纯增生、上皮异常增生和鳞状细胞癌组织所提取的核蛋白中p6 5的表达差异。结果在正常颊粘膜和上皮单纯增生组织中未见明显的p6 5表达,且两者相比无显著性差异(P >0 .0 5 )。随着上皮异常增生的出现,p6 5表达增强,较正常组和单纯增生组均有显著性差异(P <0 .0 1)。在鳞状细胞癌组织中,p6 5的表达进一步增强,与正常组和异常增生组相比均有显著性差异(P <0 .0 1)。结论p6 5在金地鼠颊囊鳞状细胞癌发生、发展过程中被激活。P6 5在金地鼠颊囊癌变过程中的表达异常,尤其是在上皮异常增生阶段的表达水平上调,可能是口腔粘膜上皮癌变过程中的一个早期事件。     

The effect of levamisole on DMBA-induced carcinogenesis in the hamster cheek pouch.

The effect of levamisole on DMBA-induced carcinogenesis in the cheek pouch of the hamster was studied. Findings indicate that levamisole modified the response of the pouch to DMBA at six weeks, while this effect was not maintained after 12 weeks of DMBA application.     

The effect of levamisole on experimental carcinogenesis of rat submandibular gland.

Sixty young adult male albino rats were studied for the effect of a non-specific enhancer of cell-mediated immunity (levamisole) on the development of chemically induced tumours of submandibular gland. Levamisole was previously shown to delay chemical carcinogenesis of hamster buccal pouch and it was felt that these findings should be confirmed in another species and another experimental tumour system. Group 1 animals received a pellet of 9,10-dimethyl-1,2-benzanthracene (DMBA) implanted into the right submandibular glands and were injected with 0.6 mg levamisole once daily on three consecutive days at intervals of two weeks. Group 2 animals were implanted with DMBA but received no levamisole. Groups 3 and 4 were levamisole-treated and untreated controls. Group 1 animals (DMBA-levamisole) showed delayed carcinogenesis of submandibular gland. Group 2 (DMBA) animals developed tumours more rapidly and they demonstrated more anaplasia and greater invasiveness than those developing in the levamisole-injected animals.     

DMBA诱发金黄地鼠颊囊癌变的动态研究——光镜部分

用光镜观察致癌剂二甲基本并蒽（ＤＭＢＡ）诱发８９只金黄地鼠颊囊在不同时期的癌变过程，其结果显示，在连续涂布０．５％ＤＭＢＡ３周后出现轻度上皮异常增生，连续涂布５周即出现中度异常增生，６周至９周均出现重度异常增生甚至原位癌，提示０．５％ＤＭＢＡ诱发金黄地鼠颊囊出现异常增生的最初时间为第３周，６周后即已发展至原位癌，也进一步提示１～３周是研究金黄地鼠颊囊出现上皮异常增生的最初阶段。     

The effect of dinitrochlorobenzene (DNCB) on dimethylbenzathracene (DMBA) carcinogenesis on hamster buccal pouch.

DNCB is an antigen that stimulates the cell-mediated response in a sensitized host. The purpose of this study was to define the effect DNCB would have on a standard model system for oral carcinogenesis, theorizing that tumorgenesis would be delayed or inhibited. Fifty-six Syrian hamsters were divided into four groups. In group A (DNCB/DMBA), the right buccal pouch of twenty animals was treated with 2% DNCB in orabase, twice a week, and 0.5% DMBA, three times a week, for 10 weeks. In group B, twenty animals received DMBA only. In group C, six animals received DMBA in Orabase. In group D, ten animals received DNCB in Orabase only. The animals were killed at 6, 12, 16, and 20 weeks. The results indicate that there were no differences in the latent period or in the histologic characteristics of the epidermoid carcinomas that developed. However, there was sensitization of the buccal pouch in pouches painted with DNCB, in that gross and histologic evidence of a delayed sensitivity reaction was demonstrated.     

前列腺素E2和白三烯B4对口腔癌促进作用的研究

目的通过在DMBA诱导的金黄地鼠口腔癌颊囊模型上,局部应用白三烯n4（LTB4）或前列腺素E2（PGE2）,探讨LTB4和PGE2在口腔癌形成中的作用,进一步阐明口腔癌的发病机制。方法用DMBA涂抹金黄地鼠左侧颊囊粘膜4周,第五周开始分别局部涂抹LTB4和PGE220周,处死动物,取全部颊囊进行肉眼观察计数和组织学检查,并进行统计学分析。结果LTB4＋DMBA组癌变率为51.4％（18／35）;PGE2＋DMBA组癌变率为45.7％（16／35）,均显著高于DMBA组20.0％（7／35）。另外两组不使用DMBA启动,同时局部分别涂抹LTB4和PGE220周,两组均有异常增生发生,但是没有癌变。结论PGE2和LTB4是口腔癌形成的促进剂,并且有可能成为化学预防作用的重要靶点。     

Effect of levamisole on the incidence and prevalence of recurrent aphthous stomatitis

Abstract Twenty patients with severe recurrent aphthous stomatitis were treated with a new drug, levamisole, to determine its effects upon the incidence and prevalence of recurrent aphthous stomatitis. The trial, conducted in a non-crossover, double-blind fashion, did not demonstrate any statistically significant differences when levamisole and placebo groups were compared over time either in the number of ulcers, number of ulcer-days, mean duration of ulcers, or mean number of ulcers per day. Positive subjective results were experienced by the 10 patients receiving levamisole but these were not of sufficient magnitude to affect group mean differences.     

Effect of DMBA on oral cancer development in hamsters with latent HSV-1 infections in trigeminal ganglia

This study examined the effect of 7, 12-dimethyl-benz(a)anthracene (DMBA), polycyclic aromatic hydrocarbon carcinogen, on pathologic changes in buccal pouch mucosa of hamsters with latent herpes simplex virus type 1 (HSV-1) infections in their trigeminal ganglia. Of the pouches receiving DMBA treatment, the average number of tumors per pouch was significantly higher (p less than 0.01) in pouches of animals with HSV-1 infections as compared to those that received DMBA only. Of the pouches receiving DMBA, the average cumulative tumor diameter (the sum of the tumor diameters in each group divided by the number of pouches in that group) was notably greater in animals with latent HSV-1 than in animals that had not been infected; however, this difference was not significant. The DMBA-treated hamsters that had latent infections also displayed a higher severity and prevalence of histopathologic changes in their pouch mucosa. This study indicates that latent HSV-1 and DMBA show synergism in the development of tumors and precancerous histopathologic changes in hamster buccal pouch mucosa. An unexpected finding was that among the HSV-1 infected animals, 95% of the ganglia from animals treated with DMBA showed latent HSV-1 virus on explanation culture, whereas only 10% of the ganglia from infected animals that received mineral oil, rather than DMBA, contained latent virus.     

Double-blind study of levamisole in aphthous stomatitis

Abstract Sixty-five patients with recurrent aphthous stomatitis participated in a double-blind study to evaluate levamisole. Nineteen of 34 patients improved on levamisole and only five of 31 patients improved on placebo (P>0.004, chi-square with two degrees of freedom). After breaking the code and switching placebo patients to levamisole and non-responders to an alternate dosage schedule, 46 of 60 patients noted clinical improvement. This is publication number 33 of the Dermatological Research Laboratories of Duke University Medical Center.     

血管新生在酒精相关性口腔癌发生中的作用

目的 观察酒精对肿瘤血管新生的促进作用,进一步阐明酒精相关性口腔癌的发病机制.方法 将105只8周龄雄性C57/6J小鼠随机分为阴性对照组、阳性对照组、8％酒精处理组.阴性对照组不做任何处理,其余2组以0.01％4NQO诱癌8周后,阳性对照组常规饲养;8％酒精组以8％酒精溶液代替饮用水饲养;共16周.实验第24周末处死...     

Double-blind study of levamisole in aphthous stomatitis.

Sixty-five patients with recurrent aphthous stomatitis participated in a double-blind study to evaluate levamisole. Nineteen of 34 patients improved on levamisole and only five of 31 patients improved on placebo (P less than 0.004, chi-square with two degrees of freedom). After breaking the code and switching placebo patients to levamisole and non-responders to an alternate dosage schedule, 46 of 60 patients noted clinical improvement.