脾酪氨酸激酶蛋白在食管鳞癌组织中的表达及临床意义

目的观察脾酪氨酸激酶（Syk）蛋白在食管鳞癌组织中的表达,探讨其与食管鳞癌发生、发展的关系。方法用免疫组化法检测48例食管鳞癌患者的癌组织及其癌旁组织中的Syk蛋白表达;分析不同TNM分期及淋巴结转移情况下食管鳞癌组织中的Syk蛋白表达。结果食管鳞癌组织Syk蛋白表达阳性率显著低于癌旁组织（χ^2=51.24,P〈0.05）;Syk蛋白表达与肿瘤TNM分期及淋巴结转移相关（P〈0.05）。结论食管鳞癌组织中Syk蛋白表达缺失与其发生及淋巴结转移倾向有关。     

ZNF282蛋白在食管鳞癌中的表达及预后的意义

目的探讨ZNF282蛋白在食管鳞癌诊断及预后的临床病理学意义。方法采用免疫组织化学染色法检测35例正常食管黏膜组织和93例食管鳞癌中ZNF282蛋白的表达情况,分析其表达与患者年龄、性别、肿瘤大小、病理学分期、分化、淋巴结转移和临床分期、生存率之间的关系。结果在正常食管黏膜组织中ZNF282蛋白主要表达于基底层细胞核内,在食管鳞癌组织中ZNF282蛋白表达于鳞癌细胞核,表达较强,其阳性表达率为47.3%;ZNF282蛋白表达在有淋巴结转移的鳞癌组明显高于无转移组(P0.05),有复发的鳞癌组明显高于无复发组(P0.01),死亡的鳞癌组明显高于生存组(P0.001);ZNF282阳性组患者生存率明显低于阴性组(P0.001);ZNF282蛋白表达与食管鳞癌患者的年龄、性别、肿瘤大小、分化、T分级、肿瘤的远处转移均无相关关系。结论 ZNF282蛋白在食管鳞癌中高表达,其表达与鳞癌淋巴结转移、肿瘤复发及患者的生存率有密切关系,可以成为诊断及判定食管鳞癌预后的重要参考指标。     

Syk基因在食管鳞癌组织中的表达及临床意义

目的:分析食管鳞癌组织及其对应癌旁组织中Syk蛋白和mRNA的表达,探讨其与食管鳞癌临床恶性生物学行为的关系.方法:采用免疫组织化学法检测48例食管鳞癌组织及其对应癌旁组织中Syk蛋白的表达;RT-PCR法检测143例食管鳞癌组织及其对应癌旁组织中PSyk mRNA的表达,并观察其与食管鳞癌患者肿瘤大小、TNM分期、淋巴结转移的关系.结果:食管鳞癌组织Syk蛋白表达阳性率显著低于癌旁组织(16.67% vs 89.58%,P<0.05);Syk蛋白表达与肿瘤删分期相关(X<'2>=6.713,P<0.05):Syk在淋巴结转移组中的阳性表达率显著低于无淋巴结转移组(3.03% vs 29.41%,P<0.05);Syk表达与肿瘤大小无关(X<'2>=0.017,P>0.05).食管鳞癌组织中Syk mRNA的表达量明显低于其在对应癌旁组织中的表达量(t=-11.27,P<0.05).结论:Syk蛋白和mRNA在食管鳞癌中表达缺失与其发生及转移倾向相关,Syk可能是食管鳞癌的肿瘤抑制基因,可能作为分子标记而用于食管鳞癌的早期诊治.     

Runx3与Smad4在食管鳞癌中表达和临床意义

目的研究Runx3与Smad4在食管鳞癌中的表达及意义。方法采用免疫组化SP法检测Runx3、Smad4蛋白在60例食管鳞癌组织,40例癌旁组织及40例正常食管鳞状上皮组织中的表达。结果食管鳞癌组织、癌旁组织及正常组织中Runx3阳性表达率,Smad4阳性表达率差异均有统计学意义(P<0.05)。Runx3和Smad4在食管鳞癌中的表达与浸润深度、淋巴结转移及临床分期(TNM)相关(P<0.05),与患者的性别、年龄、肿瘤部位、肿瘤大小及组织分化程度无关(P>0.05)。Runx3和Smad4在食管鳞癌组织中的表达呈正相关(r=0.760,P<0.05)。结论 Runx3和Smad4蛋白在食管鳞癌组织中低表达,与食管鳞癌的发生发展、侵袭转移及预后有关,可能成为食管磷癌预后不良的生物学指标之一。     

MTSS1及Gli1在食管鳞癌组织中表达及其相关性

目的 检测食管鳞癌组织中肿瘤转移抑制蛋白(MTSS)1及胶质瘤相关癌基因蛋白(Gli)1的表达水平,探讨MTSS1和Gli1的相关性。方法 应用免疫组织化学SP法和原位杂交方法检测136例食管鳞癌组织及其相应的96例癌旁不典型增生组织和136例正常食管黏膜组织中MTSS1、Gli1蛋白及mRNA的表达水平。结果 MTSS1蛋白和mRNA在食管鳞癌组织中的阳性表达率显著低于癌旁组织和正常食管黏膜组织,两两比较差异均有统计学意义(P<0.05)。Gli1蛋白和mRNA在食管癌组织中的阳性表达率显著高于癌旁不典型增生组织和正常食管黏膜组织,两两比较差异均有统计学意义(P<0.05)。有淋巴结转移组食管鳞癌组织中MTSS1蛋白及mRNA阳性表达率均显著低于无淋巴结转移组(P<0.05);有淋巴结转移组食管鳞癌组织中Gli1蛋白及mRNA阳性表达率均显著高于无淋巴结转移组(P<0.05);Gli1和MTSS1蛋白在食管鳞状上皮细胞癌组织中的表达呈负向相关(r=-0.422,P<0.05),Gli1和MTSS1 mRNA在食管鳞状上皮细胞癌组织中的表达亦呈负向相关(r...     

胃癌和癌旁组织内IL-9、CXCR1表达情况与患者病情和生存预后的相关性

目的探讨胃癌及癌旁组织内白细胞介素-9(interleukin-9,IL-9)、CXC家族趋化因子受体(CXC chemokine receptor 1,CXCR1)表达水平及其与患者病情和生存预后的相关性。方法选取79例在徐州医科大学附属医院进行诊治的胃癌患者,采取免疫组织化学SP法检测胃癌组织及癌旁组织标本IL-9、CXCR1表达,分析IL-9、CXCR1表达与患者病情及预后生存的相关性。结果共收集有效癌症组织标本79例、癌旁组织标本79例,胃癌组织内IL-9、CXCR1阳性表达明显高于癌旁组织,差异有统计学意义(P0.05)。在胃癌组织中IL-9与CXCR1表达具有相关性(P0.05)。在胃癌组织内IL-9阳性表达与浸润深度、TNM分期及淋巴结转移相关(P0.05),而CXCR1阳性表达与TNM分期及淋巴结转移相关(P0.05)。胃癌患者IL-9、CXCR1阳性表达者预后生存期明显低于阴性者,差异有统计学意义(P0.05)。将预后相关因素进行Cox多因素分析显示,淋巴结转移、TNM分期、IL-9表达、CXCR1表达是胃癌患者预后的独立影响因素(P0.05)。结论胃癌组织中IL-9、CXCR1阳性表达明显升高,IL-9、CXCR1表达与胃癌患者病情及预后相关,IL-9、CXCR1阳性表达是影响胃癌患者预后生存期的独立因素。     

CHD5与KLF5蛋白在胃癌组织中的表达及其与预后的关系

目的:探讨胃癌组织中CHD5和KLF5的表达意义及其与预后的关系.方法:收集2000-01/2007-06在昆明医学院第二附属医院手术切除的208例胃癌组织标本,采用免疫组织化学SP法检测胃腺癌组织、癌旁组织中CHD5和KLF5的表达;采用χ2检验和Fisher精确概率法分析胃癌中CHD5和KLF5的表达与临床病理指标的相关性;采用Cox比例风险模型方法进行多因素分析;应用Kaplan-Meier生存分析法判断CHD5和KLF5表达与各临床病理参数及其预后的关系.结果:胃癌组织中CHD5和KLF5呈明显低表达特点.其中,CHD5的表达与患者年龄、肿瘤分化程度、浸润深度、TNM分期、淋巴结和远处转移等有关,差异有统计学意义(P<0.05);KLF5的表达与肿瘤分化程度、浸润深度、TNM分期、淋巴结和远处转移等有关,差异有统计学意义(P<0.05).多因素分析显示,患者的生存时间主要受患者性别、肿瘤部位、组织分化程度、远处转移、TNM分期、CHD5和KLF5的异常表达等因素影响;Kaplan-Meier生存分析显示,CHD5、KLF5阴性组的中位生存期分别为(21.00mo±1.36mo)、(20.00mo±1....     

黑色素瘤抗原A1和A3蛋白在食管鳞癌组织中的表达及其对预后的判断价值

目的观察食管鳞癌组织黑色素瘤抗原(MAGE)-A1和-A3蛋白表达,以指导预后判断及临床治疗。方法 83例食管鳞癌标本为观察组,52例正常食管黏膜为对照组,应用免疫组织化学技术检测两组MAGE-A1和-A3蛋白表达。结果食管鳞癌中MAGE-A1和-A3表达阳性率明显高于正常食管黏膜组织,MAGE-A1和-A3表达与TNM分期、Ki67表达及淋巴结转移密切相关,食管鳞癌MAGE-A1和-A3表达呈正相关,且与患者生存密切相关。结论 MAGE-A1和-A3在食管鳞癌发生发展中有重要协同作用,联合检测MAGE-A1和-A3可能与食管鳞癌预后密切相关。     

HMGB1和TLR4在食管鳞癌组织中的表达及临床意义

目的:探讨高迁移率族蛋白B1(high mobility group protein B1,HMGB1)和Toll样受体4(Toll-like receptor 4,TLR4)在人食管鳞状细胞癌(食管鳞癌)组织中的表达及其临床意义.方法:选择72例食管鳞癌标本,15例癌旁正常组织标本,采用En Vision免疫组织化学染色法检测HMGB1和TLR4在食管鳞癌组织及癌旁正常组织中的表达,并应用统计学方法对其表达与临床病理因素进行分析.结果:食管鳞癌组织中HMGB1、TLR4的表达显著高于正常组织(P0.05),且与淋巴结转移及TNM分期相关(P0.05),与肿瘤大小、分化程度等无相关性.食管麟癌组织HMGB1和TLR4的表达呈显著正相关(r=0.377,P0.01).结论:食管鳞癌组织中HMGB1、TLR4的表达显著高于癌旁正常组织,且其表达与淋巴结转移及TNM分期相关,联合检测二者可能有助于评估食管鳞癌的恶性程度.因此,HMGB1/TLR信号通路有可能作为反映食管癌预后的重要生物学指标及抗食管癌的重要靶点.     

Ang-2、COX-2及CD105联合检测评价食管鳞癌患者预后的意义

目的探讨血管生成素-2(Ang-2)和环氧合酶-2(COX-2)在食管鳞癌中的表达情况。方法采用S-P免疫组化方法检测97例食管鳞癌标本及其切缘正常食管组织12例中的Ang-2和COX-2的表达情况。结果 Ang-2和COX-2在食管鳞癌组织中表达均较正常组织高(P<0.05);Ang-2的阳性表达和癌组织浸润深度、淋巴结转移及临床分期相关(P<0.05),而COX-2的表达水平与癌组织分化程度、淋巴结转移及临床分期相关(P<0.05);食管鳞癌组织中Ang-2表达与COX-2表达之间存在明显相关性(r=0.39,P=0.028)。对随访10年的97例患者进行了Kaplan-Meier生存曲线分析,Ang-2和COX-2阳性患者10年生存率分别低于Ang-2与COX-2阴性患者(P<0.05)。结论 Ang-2和COX-2的过度表达参与了食管鳞癌的发生发展过程,且两者具有正向协同性,可以作为临床肿瘤抗血管治疗的靶点和判断患者预后的参考指标。     

Original article: The expression of CFL1 and N‐WASP in esophageal squamous cell carcinoma and its correlation with clinicopathological features

Cofilin1 (CFL1) is an actin‐modulating protein, which belongs to the ADF/Cofilin family. Neural Wiskott–Aldrich syndrome protein (N‐WASP) is the key regulator of the actin cytoskeleton, a member of Wiskott‐Aldrich syndrome protein family. They have been suggested to be involved in cancer cell invasion and metastasis. In this study, the expression patterns of CFL1 and N‐WASP in normal esophageal mucosa and esophageal squamous cell carcinoma (ESCC) and their correlation with clinical characteristics were investigated. Immunohistochemical staining showed that CFL1 was expressed in nuclear and cytoplasm of cancer cells. However, N‐WASP was mainly found in the cytoplasm of the cancer cells. There were significant evidences that proved that CFL1 is correlated with clinicopathological factors in ESCC, such as infiltration depth, lymph node metastasis and pathological staging (P < 0.05). It is also proved that N‐WASP is related to lymph node metastasis and pathological staging in ESCC (P < 0.05). Kaplan–Meier analysis showed that there was no correlation between CFL1 and N‐WASP protein expression and survival (P > 0.05). Moreover, the mRNA expression of CFL1 and N‐WASP was detected by quantitative real time PCR in 70 tissue specimens. The results showed that CFL1 mRNA level was over‐expressed in ESCC tissue (P < 0.05), while N‐WASP mRNA expression level was not different between cancerous tissues and adjacent normal esophageal mucosa (P > 0.05). Also, CFL1 mRNA expression was significantly associated with regional lymph node metastasis and pathological staging (P < 0.05). Kaplan–Meier analysis showed that there was no correlation between CFL1 and N‐WASP mRNA expression and survival (P > 0.05). Our findings suggested that CFL1 and N‐WASP may play an important role in the tumorigenesis of ESCC, and to be the candidate novel biomarkers for the diagnosis and prognosis of ESCC. These findings may have implications for targeted therapies in patients with ESCC.     

食管鳞癌中Paxillin mRNA的表达及其与癌转移的关系

目的探讨Paxillin mRNA表达与食管癌转移的关系。方法采用原位杂交方法检测54例食管鳞癌、癌旁不典型增生组织及其正常食管组织中Paxillin mRNA的表达。结果有淋巴结转移的21例（A组）食管鳞癌组织、癌旁不典型增生组织和正常食管组织中Paxillin mRNA阳性表达率分别为90．48%（19／21）、52．38％（11／21）和0，无淋巴结转移的33例（B组）食管鳞癌组织、癌旁组织和正常食管组织中的Paxillin mRNA阳性表达率分别为33．33％（7／33）、23．80％（5／33）和0。两组癌组织、癌旁不典型增生组织中Paxillin mRNA阳性表达率比较，P均〈0．05。结论Paxillin mRNA表达与食管鳞癌的转移密切相关。     

肝素酶基因表达与食管癌转移的关系

目的　探讨肝素酶基因表达与食管癌转移的关系。方法　采用逆转录聚合酶链反应技术 ( RT-PCR)检测 5 4例食管癌组织、癌旁组织及正常粘膜中肝素酶基因的表达。结果　有淋巴结转移的食管癌癌组织中肝素酶基因阳性表达率为 90 .5 % ( 19/ 2 1) ,癌旁组织为 6 1.9% ( 13/ 2 1) ,正常粘膜为 0 ( 0 / 2 1)。无淋巴结转移的食管癌癌组织中肝素酶基因阳性表达率为 15 .2 % ( 5 / 33) ,癌旁组织为 6 % ( 2 / 33) ,正常粘膜为 0 ( 0 / 33)。有淋巴结转移的食管癌组织及癌旁组织中肝素酶基因阳性表达率均高于无淋巴结转移者 ,二者相比差异均有显著性 (癌组织 P<0 .0 0 1,癌旁组织 P<0 .0 5 )。结论　肝素酶基因表达可能与食管癌发生转移有关 ,其可能成为预测食管癌转移的指标     

15-LOX-1蛋白在大肠癌中的表达及临床意义

目的探讨15-LOX-1蛋白表达与大肠癌临床病理因素和患者预后的关系,并探索其可能的作用机制。方法采用免疫组化SP法,检测15-LOX-1蛋白在103例大肠癌组织、56例大肠癌癌旁正常组织中的表达,结合患者的临床病理因素、预后情况及大肠癌组织中其他与侵袭转移相关的指标进行分析。结果 15-LOX-1蛋白在大肠癌组织中的阳性表达率显著低于大肠癌癌旁正常组织(P<0.05);15-LOX-1蛋白的表达与大肠癌的组织病理类型、淋巴结转移、其他器官侵袭转移以及Dukes分期密切相关(P<0.05),病理分化程度低、Dukes分期晚以及有淋巴结或其他器官侵袭转移者肠癌组织中15-LOX-1蛋白表达水平降低;大肠癌组织中15-LOX-1阳性表达患者1年、3年、5年生存率及中位生存时间明显高于15-LOX-1阴性表达患者(P<0.05),多因素COX回归分析结果提示15-LOX-1表达水平、患者年龄及手术时有无淋巴结转移可以作为评估大肠癌患者预后的独立因素(P<0.05);大肠癌中15-LOX-1与VEGF、MMP-2、MMP-7的表达均呈负相关(P<0.05)。结论 15-LOX-1对大肠癌具有抑癌作用,对反映大肠癌生物学行为和判断预后有重要意义。     

食管鳞癌组织中VEGF-D表达及其与淋巴管密度和淋巴转移的关系

目的 研究食管鳞状细胞癌(ESCC)组织中血管内皮细胞生长因子D(VEGF-D)的表达情况及其与淋巴管密度(LVD)的关系,并探讨其与食管癌淋巴结转移的关系.方法 免疫组化SABC法检测55例手术切除的食管鳞状细胞癌组织、癌旁组织、正常组织中淋巴管密度和VEGF-D的表达水平,对结果进行统计分析.结果 (1)食管癌组织...     

Expression of vascular endothelial growth factor-C and angiogenesis in esophageal squamous cell carcinoma

AIM: To investigate the expression of vascular endothelial growth factor-c (VEGF-C) mRNA and microvessel density (MVD) in human esophageal squamous cell carcinoma (ESCC) and its relationship with clinical significance. METHODS: Specimens obtained from 43 patients undergoing surgical resection for ESCC were used in this study. The expression of VEGF-C mRNA was examined by in situ hybridization. Tumor MVD was determined immunohistochemically with anti-CD31 antibody and estimated by image analysis. Ten sections of adjacent normal mucosa were also examined. RESULTS: VEGF-C mRNA expression was detected in cytoplasm of carcinoma cells. Of the 43 ESCC patients studied, 18 cases (41.9%) were positive for VEGF-C mRNA. No VEGF-C mRNA expression was observed in normal esophageal mucosa. VEGF-C mRNA expression correlated significantly with lymph node metastasis, TNM stage and depth of invasion (P < 0.05). Furthermore, histological grade (differentiation) tended to correlate with VEGF-C mRNA expression, but was not statistically significant (P > 0.05). In tumor lesions, the MVD was significantly greater than that in normal esophageal mucosa. MVD correlated significantly with lymph node metastasis, TNM stage and depth of invasion (P < 0.05), but not with histological grade (differentiation) (P > 0.05). Lesions with VEGF-C mRNA expression had a significantly higher MVD than that of those without VEGF-C mRNA expression (P < 0.05). CONCLUSION: VEGF-C plays a role in lymphatic metastasis via lymphangiogenesis and angiogenesis in ESCC. VEGF-C is one of the important predictors of the biological behavior in ESCC.     

The expression of CXCR4 and its relationship with matrix metalloproteinase‐9/vascular endothelial growth factor in esophageal squamous cell cancer

Esophageal cancer (EC) is a highly aggressive neoplasm with poor prognosis. The main reason for this disappointing outcome is the strong behavior of esophageal cancer cell's invasion and metastasis. CXC chemokine receptor 4 (CXCR4) was found to be expressed in many tumors and significantly correlated with invasion, angiogenesis, metastasis, and prognosis. In the present study, we investigated the expressions of CXCR4, matrix metalloproteinase‐9 (MMP‐9), and vascular endothelial growth factor (VEGF) in esophageal squamous cell cancer (ESCC) and analyzed the relationship among the three proteins. Sections of paraffin‐embedded tissues were obtained from 127 patients with ESCC undergoing esophagectomy at Zhongshan Hospital, Fudan University in 2005. The CXCR4, MMP‐9, and VEGF expressions in EC tissues were evaluated according to the immunohistochemical staining area and intensity. The correlations between patients' prognosis and covariates were analyzed by Kaplan–Meier method (univariate analysis) and Cox regression (multivariate analysis). The overall expression rate of CXCR4, MMP‐9, and VEGF was 88.2%, 93.7%, and 79.5%, respectively. CXCR4 expression was significantly associated with tumor grade, tumor size, tumor depth, regional lymph node metastasis, and tumor, node, metastasis (TNM) stage (P < 0.05). MMP‐9 expression was significantly associated with age and tumor grade (P < 0.05). VEGF expression was significantly associated with tumor grade, tumor depth, and TNM stage (P < 0.05). CXCR4 expression was positively correlated with MMP‐9 expression (P < 0.01, r= 0.365) and VEGF expression (P < 0.01, r= 0.380). However, there was no significant correlation between MMP‐9 and VEGF expression (P > 0.05). In univariate analysis, CXCR4 expression, tumor size, tumor depth, lymph node metastasis, and TNM stage were correlated with patients' prognosis (P < 0.05); in multivariate analysis, tumor size and lymph node metastasis were the independent factors of poor prognosis. CXCR4 was highly expressed in ESCC and correlated with MMP‐9, VEGF, clinicopathological features and prognosis. We speculated CXCR4 play an important role during the progression of this disease and there might be some regulatory mechanism existing between CXCR4 and MMP‐9/VEGF in ESCC.     

Correlation between periostin and SNCG and esophageal cancer invasion,infiltration and apoptosis

ObjectiveTo investigate the correlation between periostin and SNCG and esophageal cancer invasion, infiltration and apoptosis.MethodsA total of 78 cases esophageal surgical resection specimens were collected, expression of periostin and SNCG in esophageal cancer were detected. Effect of periostin and SNCG in esophageal carcinoma invasion and infiltration was analyzed.ResultsThe upregulated rate of periostin had significant difference in esophageal cancer tissues (39.74%), adjacent tissues (17.86%) and normal tissues (0.00%); The positive expression rates of SNCG had significant difference in esophageal cancer tissues (61.54%), adjacent tissues (32.14%) and normal tissues (1.96%); The upregulated rate of periostin had a significant correlation with lymph node metastasis, adventitia invasion, TNM stage; The positive expression rates of SNCG had a significant correlation with differentiation degree, lymph node metastasis, adventitia invasion, TNM stage; Apoptosis index of the positive of expression of SNCG of esophageal cancer tissue (4.541±2.267) was significantly lower than that of the negative expression (7.316±2.582) (P<0.05).ConclusionsSNCG may play an important role in invasion, infiltration and apoptosis of esophageal cancer and serve as target spots in the targeted therapy of esophageal cancer.     

cystatin B基因在人食管癌中的表达

目的：研究cystatinB基因在人食管癌中的表达状况。方法：采用逆转录聚合酶链反应（RT－PCR）方法检测41对食管癌组织及配对食管癌旁黏膜和食管癌细胞系EC9706和肺癌细胞系GLC82中cystatinB基因的表达。结果：cystatinB基因在82．95（34／41例）食管癌组织中的表达水平,低于配对的食管癌旁黏膜,且与食管癌淋巴结转移显著相关（P＜0．05）。在食中细胞系EC9706有微弱表达,在肺癌细胞系GLC82未检测到表达。结论：cystatinB基因在人食管癌中表达显著下调且与淋巴结转移相关。