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1.
张文敏 《福建医科大学学报》1996,(4)
目的研究30例肌源性肌病,包括进行性肌营养不良症(PMD)、多发性肌炎(PM)/皮肌炎(DM)、先天性肌病(CMD)和代谢性肌病(MMD)骨骼肌活检组织的体液免疫、细胞免疫与免疫调控等免疫活性表达,探讨其临床意义及与病变之间的关系。方法免疫酶组化SP法检测抗体CD3、CD4、CD8、CD68和HLA-DR,免疫荧光一步法检测免疫球蛋白IgG、IgM和补体C3。结果(1)PMD肌组织中免疫球蛋白IgM、IgG和补体C3免疫复合物的阳性率分别为50%,31.1%和11.1%,以IgM有统计学意义(P<0.05),主要定位于肌膜和间质小血管壁上,并与PMD肌纤维的萎缩性病变有关。(2)PMD肌组织中有单核炎症细胞浸润,以巨噬细胞和T淋巴细胞为主,其阳性反应率分别为100%和55.5%。巨噬细胞多位于坏死纤维中,T淋巴细胞分布于变性坏死肌纤维和肌束衣小血管周围,CD8+T细胞数量增多,T4/T8<1。(3)HLA-DR染色显示PMD肌组织中变性萎缩的小纤维周围有HLA-DR+的激活单核炎症细胞浸润,与神经源性肌病组及正常对照组比较差别均有显著性(P<0.05)。肌纤维HLA-DR阳性反应者占22%(4/18),表明 相似文献
2.
检测柯萨奇B组病毒(CVB)性心肌炎70例,正常人(NC)40例外周血的自然杀伤细胞(NKC)活性和T细胞亚群。结果显示病毒性心肌炎(VMC)组NKC活性显著低于NC组(P〈0.01)。T细胞亚群与NC组比较,急性VMC患者总T细胞(CD3),辅助性T细胞(CD4)和抑制性T细胞(CD8)及CD4/CD8比值均减少,P〈0.05 ̄0.01。慢性VMC患者仅CD8减低明显(P〈0.05),而CD3、 相似文献
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4.
为探讨卵巢恶性肿瘤(MOT)与细胞免疫的关系,对40例MOT患者和30例正常对照(NC)妇女的T淋巴细胞亚群和白细胞介素-2(IL-2)、可溶性白细胞介素-2受体(sIL-2R)进行了同步检测及相关性研究。结果:MOT组IL-2、CD+3、CD+4、CD+4/CD+8均低于NC组,sIL-2R、CD+8均高于NC组(P<0.01~0.001),其中CD+3、CD+4、CD+4/CD+8与IL-2呈正相关,与sIL-2R呈负相关(P<0.01~0.001)。提示本病患者T淋巴细胞免疫功能低下,CD+3、CD+4、CD+4/CD+8值的降低与IL-2活性下降和sIL-2R水平增高有关,它们均可能是本病发生发展的重要因素。 相似文献
5.
乙肝病毒感染者T细胞亚群状态与肾脏损害的关系 总被引:8,自引:0,他引:8
目的:探讨T细胞亚群变化在乙肝病毒相关肾炎发病中的作用。方法:利用流式细胞仪,检测30 例乙肝病毒感染后发生肾损害者周围血CD4+ 、CD8 + 细胞的变化;并对6 例经肾活检证明为乙肝病毒相关肾炎患者用免疫组化ABC法检测肾组织CD4+ 、CD8 + 细胞浸润情况。结果:乙肝病毒感染后发生肾损害组周围血CD8+ 细胞明显升高(P< 0.05);CD4+ /CD8 + 明显下降(P< 0.05),与24 h 尿蛋白定量呈负相关( r= - 0.5596,P< 0.05);乙肝病毒相关肾炎患者肾组织CD4+ 、CD8 + 细胞数明显增加( P< 0.05)。结论:乙肝病毒感染后发生肾损害者存在周围血T细胞亚群失衡;不仅特异性体液免疫,而且特异性细胞免疫及非特异性免疫机理也参与乙肝病毒相关肾炎发病 相似文献
6.
丙肝患者中血T细胞亚群及mIL—2R的检测 总被引:4,自引:2,他引:2
目的:探讨丙型毒性肝炎(丙肝)患者的细胞免疫水平及其在丙肝发病机理中的作用。方法:用生物素-链霉订和素法对112例-HCV阳性患者中血单个核细胞进行T细胞亚群及植物血凝素(PHA)诱导前后mIL-2R的检测。结果:急慢性丙肝患者与正常对照相比,外周血CD3^+、CD4^+百分率降低,CD8^+百分率增高,CD4^+/CD8^+比值下降(P〈0.05~P〈0.01)。外周血单个核细胞在青息状态和在P 相似文献
7.
为探讨胚胎胸腺细胞(FTC)移植的作用机理,采用单克隆抗体免疫荧光法检测了19例淋巴细胞癌变患者FTC移植前后T淋巴细胞亚群分布,结果表明:FTC移植前患者CD^+3,CD^+4,CD^+4/CD^+8低于正常对照组(P〈0.01),CD^+8高于对照组(P〈0.01);FTC移植后CD^+3,CD^+,较移植前明业增高(P〈0.01),CD^+8显著降低(P〈0.01),CD^+4/CD^+8, 相似文献
8.
丙肝患者外周血T细胞亚群及mIL-2R的检测 总被引:5,自引:0,他引:5
目的:探讨丙型病毒性肝炎( 丙肝) 患者的细胞免疫水平及其在丙肝发病机理中的作用。方法:用生物素链霉亲和素法对112 例抗 H C V 阳性患者外周血单个核细胞进行 T 细胞亚群及植物血凝素( P H A) 诱导前后m I L2 R 的检测。结果:急慢性丙肝患者与正常对照相比,外周血 C D3 + 、 C D4 + 百分率降低, C D8 + 百分率增高, C D4 + / C D8 + 比值下降( P< 0 .05 ~ P< 0 .01) 。外周血单个核细胞在静息状态和在 P H A 诱导状态,其m I L2 R 表达水平与正常对照组相比均低下( P< 0 .01) ,但急慢性丙肝患者中 T 细胞亚群及m I L2 R 表达水平类似( P> 0 .05) 。结论:丙肝患者体内存在明显的细胞免疫功能紊乱, T 细胞活化障碍,并与肝病的慢性化有关。 相似文献
9.
李凤玲 《右江民族医学院学报》1995,(3)
测定48例NIDDM患者的外周血T淋巴细胞亚群,结果显示CD_3、CD_4、CD_8值均降低,与正常对照组比较,差异有高度显著性(P<0.01)。提示NIDDM患者有T淋巴细胞功能缺陷。并讨论NIDDM患者T细胞亚群变化的可能原因。 相似文献
10.
用直接SPA菌花环法体外测定13例骨髓增生异常综合征(MDS)患者外周血T细胞亚群,结果表明:患者平均OKT+4细胞百分值、OKT+4/OKT+8比值均显著低于正常(P<0.05~0.002),平均OKT+8细胞百分值显著高于正常(P<0.005)。说明MDS患者淋巴细胞亚群分布异常。并发现T细胞亚群随病情发展而变化,提示外周血T细胞亚群的变化可以作为判断病情,预后及疗效的辅助参考指标之一。 相似文献
11.
Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P < 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (>0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P <0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P < 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD. 相似文献
12.
Jiageng Zhu Jun Li Ruipeng Ji Jianghao Su Mingshun Shen Zhigang Cao 《南京医科大学学报(自然科学版)》2007,21(2):125-128
Objective: To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods: SD rats served as both donors and recipients. 4℃ sodium lactate Ringer's was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava (IVC) inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results: Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion: This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment. 相似文献
13.
Tao Ye * Zhi-quan Liu Jian-jun Mu Xi-han Fu Jun Yang Bao-lin Gao and Xiao-hong Zhang Department of Cardiology First Hospital of Xi'an Jiaotong University Xi'an Hanzhong Cardiovascular Institute Hanzhong 《中国医学科学杂志(英文版)》2004,19(4):248-251
Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P< 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P< 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure. 相似文献
14.
目的:评价使用安心颗粒对急诊经皮冠状动脉介入术(PPCI)术后生活质量的影响.方法:将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组(术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次)和对照组(仅接受基础药物治疗).所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表(MIDAS)、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果:入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异(P>0.05);出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低(P<0.05);组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低(P<0.05).两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血(P>0.05).两组发生轻度血小板减少症的患者数比较无差异(P>0.05).结论:PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险. 相似文献
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Jingming Zhu Ningning Hui Xiuhong Jiang Huaxing Zhang Chengzhang Fu 《南京医科大学学报(自然科学版)》2007,21(6):386-389
Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P<0.01);the MAP decreased(P<0.01)and the HR increased drastically(P<0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients. 相似文献
16.
Objective:To investigate the gene expression of osteoprotegerin(OPG) and osteoclast differentiation factor(ODF) in the bone tissue of patients with hip fracture due to osteoporosis. Methods:OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers(over 50 years old) with hip fracture(Observer Group) and 30 cases of hip facture sufferers with no osteoporosis(Control group) were analyzed with the Semi-Quantitative RT-PCR method. Results:The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion:Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption. 相似文献
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The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas: report of 9 cases 总被引:1,自引:0,他引:1
Shaoqin Chen Shengquan Zou Qibao Dai Peisheng Huang Weihua Cai Hong Li 《南京医科大学学报(自然科学版)》2007,21(6):398-401
Objective:To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas(SPTP). Methods:Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin(HE) staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results:Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin(9/9 cases), AAT(9/9 cases), NSE(9/9 cases), ACT(7/9 cases), CK20(2/9 cases), CgA(1/9 cases), S-100(3/gcases), PR(4/gcases), Syn(3/9 cases) and CD56(5/9cases), negative in CEA and ER. Conclusion:SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype. 相似文献
18.
Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine
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A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care(~'4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes. 相似文献
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Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P. 相似文献
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Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine. 相似文献