拔牙后牙槽窝立即植入羟基磷灰石微粒人工骨预防牙槽嵴吸收的临床观察

目的：研究羟基磷灰石微粒人工骨对拔牙后牙槽骨吸收的疗效及并发症。方法：60例患者拔牙后牙槽窝内立即植入羟基磷灰石微粒骨，术后1周，1个月，4个月分别复诊。进行临床及X线检查。结果：60例患者伤口均愈合良好。未见并发症；同术前相比，牙槽骨高度降低不明显。结论：羟基磷灰石微粒人工骨可有效地预防拔牙后牙槽骨的吸收，可为义齿修复及种植牙提供良好的条件。     

羟基磷灰石人工骨填塞拔牙创保持牙槽嵴高度的临床研究

目的 对拔牙术后押制牙槽骨过度吸收,保持牙槽嵴高度研究。方法 造反例需同时拔除下凳两侧相同部位牙齿的患者,按左右分级,一侧为拔牙后空白对照,另一侧拔牙后立即填塞颗粒型羟基磷灰石人工骨。即刻、１２周、２４周分别摄Ｘ线片,测量两级牙槽嵴高度结果创口Ⅰ期愈合３１例,延期愈合２例。两级比较,填塞组牙槽嵴高度明显高于对照组。结论羟基磷灰石人工骨填塞拔牙创可以其支架作用替代牙齿对牙槽骨的支持作用,抑制拔牙后     

拔牙后植入珊瑚骨复合替硝唑的疗效观察

目的 通过观察拔牙后植入珊瑚人工骨复合替硝唑的治疗效果，探索如何减少拔牙后牙槽嵴的萎缩和吸收，有效地维持牙槽嵴的高度和宽度，为后续的义齿修复奠定良好的解剖学基础。方法 对76例患者拔牙后植入珊瑚人工骨复合替硝哇于牙槽窝内，术后1周、1个月、3个月分别复诊并行X线摄片检查．结果 76例拔牙患者伤口愈合良好，未发现术后出血、裂开及感染，无植入物排异等并发症；牙槽嵴平滑、无凹陷；同术前相比，牙槽嵴高度无明显降低。结论 拔牙后植入珊瑚人工骨复合替硝哇可战少术后并发症、维持牙槽嵴的有效高度，为后续的义齿修复提供了基骨条件。     

局部性牙槽骨萎缩的牙槽骨扩展术

本研究的目的是观察运用致密羟磷灰石微粒，在颌骨萎缩患者牙槽嵴上行牙骨扩展术的临床效果，采用牙槽骨扩展术在２０例颌骨萎缩患者的牙髓嵴上人工制成窝洞，填塞致密羟基磷灰石微粒人工骨，结果，切口１期愈合，无一例无发症，植入羟基磷灰石与基骨结合牢固，所形成的牙槽嵴无棘状突起，术后４～６周安装义齿，功能和美容效果满意，用羟基磷灰石微粒行牙槽骨扩展术，效果肯定，技术操术简单。     

拔牙创使用人羊膜的临床研究

目的:观察人羊膜植入临床拔牙创后对牙槽骨愈合的影响。方法:选用来本院需拔除双侧下颌第二前磨牙正畸治疗的患者20例,右侧拔牙创植入人羊膜作为试验组,左侧拔牙创作为空白对照。术后1、2、3个月拍口腔全景片,观察新骨形成情况及牙槽嵴吸收评价拔牙创愈合情况。结果:术后1周牙龈愈合及术后1、2、3个月拔牙创新骨形成量试验组优于对照组,术后1、2、3个月牙槽嵴吸收高度试验组均明显低于对照组(P0.01);每月牙槽嵴吸收量第1、2个月试验组均低于对照组(P0.01),第3个月两组无明显差异(P0.01)。结论:临床拔牙创植入人羊膜能促进拔牙创的早期愈合,保存牙槽嵴高度。     

60例羟基磷灰石人工骨植入牙槽窝预防干槽症的研究

本研究选60例临床拔牙后的患者，其中30例拔牙创内植入粉沫状羟基磷灰石人工骨，另30例以血液充满后处置拔牙创。术后1、3、6、9个月复查，经临床及X线片检查证明人工骨植入拔牙创能预防拔牙创感染，较传统的处理方法优越。     

Bioss和羟基磷灰石骨粉在种植外科牙槽窝位点保护中的应用研究

目的:利用牙槽窝保护技术,在拔牙后牙槽窝中即刻植入Bioss骨粉或羟基磷灰石骨材料。后期完成种植手术,同期取牙槽窝骨标本,行影像学及组织学检测,对比不同材料保护牙槽窝的临床意义。方法:选择本院口腔颌面外科门诊患者50例,因创伤致牙脱位、断裂,牙槽窝骨折者25例,牙周病15例,残根伴根尖囊肿6例,咬合致牙折4例。患者在局麻下微创拔除病灶牙根,刮净牙槽窝,分别植入Bioss骨粉或羟基磷灰石骨粉,表面用可吸收胶原膜覆盖,严密缝合创口。5⁶月后局麻下用环钻钻取牙槽窝上方骨柱,行硬组织切片检测,对比新骨形成和人工骨代谢情况。结果:50例患者中,牙槽窝骨质均得到有效保护,牙槽嵴的宽度均满足临床种植需求。刚植入人工骨粉时,数字牙片显示牙槽窝的骨密度高,人工骨颗粒清晰,到3个月时,骨粉密度渐变淡,颗粒也渐渐变得模糊。由标本硬组织切片来看,无论是羟基磷灰石还是Bioss人工骨粉,在牙槽窝中均有新骨形成,在人工骨粉颗粒间有骨质形成,越往根部,骨质形成越多,骨结构越成熟。Bioss骨粉植入的牙槽窝标本,其新骨形成量多,人工骨代谢更快。结论:利用牙槽窝保护技术,将Bioss或羟基磷灰石人工骨粉在拔牙后即刻植入牙槽窝中,均可以有效保护牙槽骨量,维持牙槽骨形态,顺利完成种植手术,软硬组织美学效果良好。Bioss骨粉的成骨效果好于羟基磷灰石人工骨粉。     

复合自固化磷酸钙人工骨修复牙槽骨缺损的临床初步探讨

目的：通过,临床试验研究自固化磷酸钙人工骨（ACPC）及其与骨形成蛋白（BMP）复合后修复牙槽骨拔牙创缺损的效果。方法：临床中将ACPC／BMP即刻植入患者拔牙创,通过临床观察和CCD数字化摄片随访至术后12周。结果：临床试验中,试验组患者牙槽嵴骨量吸收少。外形维持较好。结论：ACPC／BMP人工骨具有良好的组织相容性,即刻植入合适患者的拔牙创有利于增加牙槽骨量,维持牙槽外形。     

引导骨再生膜技术在牙槽嵴位点保存的临床疗效观察

目的探讨牙周病拔牙后应用引导骨再生膜技术在牙槽嵴位点保存的临床疗效。方法22例32颗具有拔牙适应证的患牙拔除后随机分为植骨组和非植骨组,植骨组即刻在拔牙创窝内填塞羟基磷灰石生物陶瓷骨替代材料,覆盖口腔生物膜,周围软组织松弛后拉拢缝合,严密关闭拔牙创;非植骨组常规处理拔牙创面。结果植骨组16个拔牙位点,牙槽嵴位点骨形成有效位点数为15个,未见明显骨质形成位点1个;非植骨组16个拔牙位点,牙槽嵴位点骨形成有效位点数为9个,未见明显骨质形成位点共7个。结论采用羟基磷灰石生物陶瓷充填骨缺损区同时覆盖生物膜的引导骨再生技术可获得良好的骨引导再生效果。     

羟基磷灰石微粒人工骨在口腔颌面外科的临床应用

本文报告了用羟基磷灰石微粒人工骨植入整复上领齿槽突裂、增高牙槽嵴、矫正鞍鼻、颏成形等31例,均取得理想效果。本组临床效果表明,该种新型人工骨是一种相容性优良的生物材料。对羟基磷灰石的效果、使用范同及注意事项等问题进行了讨论。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.