外源性结缔组织生长因子对人肾小管上皮细胞转分化及细胞外基质合成的作用

目的:探讨外源性结缔组织生长因子(CTGF)在人肾小管上皮细胞转分化及细胞外基质合成中的作用.方法:将体外培养的人肾小管上皮细胞HK2分为三组:对照组;CT-GF小剂量组(终浓度为2.5 μg/L);CTGF大剂量组(终浓度为20 μg/L).用倒置显微镜观察细胞形态学变化;噻唑蓝(MTr)法检测细胞增殖活性;RT-PCR检测HK2细胞E-钙黏蛋白(E-cadherin),α-平滑肌肌动蛋白(α-SMA),纤连蛋白(FN)和胶原ⅠαmRNA水平的变化;免疫组织化学方法观察HK2细胞FN和胶原Ⅰ的表达.结果:CTGF刺激使HK2细胞由椭圆形变为梭形,同时促进HK2细胞增殖.不同浓度的CTGF作用于HK2细胞48 h后,α-SMA和FN mRNA水平显著升高(P＜0.05),E-钙黏蛋白mRNA的表达显著下降(P＜0.05);大剂量CTGF刺激HK2细胞48 h后胶原ⅠαmRNA水平显著升高(P＜0.05).小剂量CTGF组HK2细胞胞质FN表达水平显著增加(P＜0.05),大剂量CTGF组HK2细胞胞质表达FN和胶原Ⅰ均显著增加(P＜0.05).结论:CTGF在体外能够诱导人肾小管上皮细胞转分化,并促进FN的合成,大剂量的CTGF可以增加其胶原Ⅰ的合成.     

IGF-Ⅰ对人肾小管上皮细胞转分化相关基因表达的影响

目的:探讨胰岛素样生长因子-Ⅰ(IGF-Ⅰ)在人肾小管上皮细胞转分化中的作用。方法:将体外培养的人肾小管上皮细胞(HK2)分为对照组和IGF-Ⅰ组:培养液中加入IGF-Ⅰ,终浓度分别为25、50、100、200ng/ml。倒置显微镜观察细胞形态变化。用逆转录-聚合酶链反应(RT-PCR)技术测定HK2细胞E-钙黏蛋白、角蛋白、α-平滑肌肌动蛋白(α-SMA)、波形蛋白和纤连蛋白(FN)mRNA水平的变化。结果:IGF-Ⅰ刺激使HK2细胞形态由椭圆变为梭形。不同浓度的IGF-Ⅰ作用于HK2细胞48h后E-钙黏蛋白和角蛋白mRNA的表达显著下降(P<0.05),α-SMA、波形蛋白和FNmRNA水平显著升高(P<0.01),且呈剂量依赖性。结论:IGF-Ⅰ在体外能刺激人肾小管上皮细胞向肌成纤维细胞(MyoF)转分化,并促进其合成细胞外基质(ECM)。     

CTGF反义寡核苷酸对AngⅡ诱导的人近曲肾小管上皮细胞转分化的影响

目的：探讨结缔组织生长因子（CTGF）反义寡核苷酸对血管紧张素Ⅱ（AngⅡ）诱导的肾小管细胞转分化的可能影响.方法：采用体外培养的人近曲肾小管上皮细胞株（HK2）,分别观察CTGF反义寡核苷酸和AngⅡ干预细胞对细胞CTGF mRNA和蛋白质的表达、细胞内超微结构及细胞α-平滑肌肌动蛋白（α-SMA）表达的影响.结果：AngⅡ干预HK2细胞48 h后,细胞CTGF mRNA和蛋白的表达显著增高（P＜0.01）;AngⅡ干预96 h后,细胞超微结构显示出间质细胞样结构;这些作用都可被CTGF反义寡核苷酸显著抑制.AngⅡ可以呈时间依赖性地刺激HK2细胞表达α-SMA,这些作用可以被CTGF反义寡核苷酸显著抑制（P＜0.01）.对照组错义寡核苷酸无上述作用.结论：CTGF反义寡核苷酸对AngⅡ诱导HK2细胞转分化具有显著的抑制作用,提示CTGF可能介导了AngⅡ诱导的肾小管上皮细胞转分化.     

内源性结缔组织生长因子介导转化生长因子β1对肾小管上皮细胞的转分化效应

目的：探讨内源性结缔组织生长因子（CTGF）在人肾小管上皮细胞（HK2）转分化过程中的作用。方法：将HK2细胞分为4组：对照组；转化生长因子β1（TGF-β1）5ng／ml刺激组；TGF-β1 5ng／ml刺激4-CTGF反义0DN 3 mmol／L干预组；TGF-β1 5ng／ml刺激＋CTGF正义ODN3mmol/L对照组。用倒置显微镜观察各组细胞的形态学变化，用RT-PCR检测各组细胞中CTGF、上皮细胞钙粘蛋白（E-cadherin）、α-平滑肌肌动蛋白（α-SMA）、纤连蛋白（FN）mRNA表达变化。结果：TGF-β1刺激使HK2细胞形态由椭圆形转为梭形；下调E-cadherinmRNA表达。上调CTGF、α-SMA和FNmRNA表达．且CTGFmRNA表达变化在时间上早于E-cadherin、α-SMA和FNmRNA表达变化；CTGF反义ODN可对抗TGF-β1引起的细胞形态学改变和E-cadherin、CTGF、α-SMA、FiNmRNA表达变化效应。结论：内源性CTGF介导了TGF-β1对HK2细胞的转分化效应。     

环孢素A对肾小管上皮细胞转分化及CTGF表达的影响

目的 观察环孢素A(CSA)对人近端肾小管上皮细胞(HK-2)细胞转分化(EMT)及结缔组织生长因子(CTGF)表达的影响,探讨其致肾间质纤维化作用的可能机制.方法 用相差显微镜观察不同浓度(0.5～25.0mg/L)CsA作用下HK-2细胞形态的变化;免疫荧光、RT-PCK、Western Blot分别检测5mg/LCsA刺激细胞不同时间(24～72h)α-SMA在细胞内的表达和分布以及α-SMA、CTGF mRNA和蛋白的表达.结果 显微镜下可见,HK-2细胞随CsA刺激浓度的增加由立方形铺路石样逐渐转变为梭形长条样.免疫荧光显示,随CsA刺激时间的延长细胞质中α-SMA表达逐渐增强.RTPCR和Western Blot显示,CsA呈时间依赖性上调α-SMA、CTGF mRNA和蛋白的表达,24h表达即明显增加,72h增加更为显著(P<0.01);CTGF与α-SMA表达的时间效应一致.结论 环孢素A可上调CTGF的表达、诱导肾小管上皮细胞转分化,进而促进肾间质纤维化.     

干扰素-γ对肾小管上皮细胞转分化的作用

目的 应用转化生长因子-β1(TGF-β1)诱导肾小管上皮细胞肌纤维母细胞转分化(TEMT),研究干扰素-γ(IFN-γ)对肾小管上皮细胞的表型重塑的作用和机制.方法 将正常大鼠肾小管上皮细胞(NRK52E)分为空白组、诱导组(TGF-β13 ng/mL)、药物组(IFN-γ1000 IU/mL)及阻断组(TGF-β13 ng/mL IFN-γ200、400、600、1000、2000、3000 U/mL),培养72 h后,观察α-平滑肌肌动蛋白(α-SMA)以及结缔组织生长因子(CTGF)的表达;测量α-SMA阳性细胞百分数和平均荧光强度(MCF),α-SMA mRNA,CTGF mRNA的表达;并测定上清液中胶原Ⅲ的含量.结果 ①诱导组细胞转分化为肌成纤维细胞样细胞,α-SMA阳性细胞百分数及MCF均明显增加(P<0.05);α-SMA mRNA和CTGF mRNA表达也明显增加(P<0.05);胶原Ⅲ含量升高(P<0.05).②药物组的细胞形态、α-SMA mRNA、CTGF mRNA表达及胶原Ⅲ含量等与空白组的差异无统计学意义(P>0.05);⑨阻断组IFN-Υ明显抑制了TGF-β1诱导的转分化,且呈剂量依赖;并抑制胶原Ⅲ合成P<0.05.结论 IFN-Υ能够负性调控TGF-β1诱导的TEMT,减少胶原Ⅲ分泌;IFN-Υ对TEMT的负性词节作用可能是通过减少CTGF表达来实现的.     

肝细胞生长因子对转化生长因子诱导肾小管上皮细胞转分化的影响

目的应用转化生长因子β1(TGF-β1)诱导肾小管上皮细胞肌纤维母细胞转分化,研究肝细胞生长因子(HGF)对肾小管上皮细胞的表型重塑的作用和机制。方法在正常大鼠肾小管上皮细胞(NRK52E细胞株)培养液中分别加入TGF-β1及HGF,应用倒置相差显微镜、扫描电镜观察细胞形态变化;免疫组织化学方法检测肌纤维母细胞特异性标志物α-平滑肌肌动蛋白(α-SMA)的表达;ELISA法分析测定培养细胞上清液中纤维连结蛋白(FN)的含量;RT-PCR检测结缔组织生长因子(CTGF)mRNA表达。结果1TGF-β1诱导肾小管上皮细胞从原有典型的上皮细胞形态转变为长梭形肌成纤维细胞样形态;α-SMA的表达、上清液FN含量、细胞CTGFmRNA表达明显增加(P<0.05)。2单纯HGF对细胞形态学、α-SMA的表达及FN含量与空白对照组无明显影响;不同浓度HGF组的CTGFmRNA表达较空白对照组均增加(P<0.05)。3HGF能抑制TGF-β1诱导的NRK52E细胞形态学改变;α-SMA的表达、FN水平均比诱导组TGF-β1明显下降(P<0.05);HGF抑制了TGF-β1诱导的CT-GFmRNA表达(P<0.05)。结论HGF能够负性调控TGF-β1诱导的肾小管上皮细胞肌纤维母细胞转分化作用,减少FN分泌;HGF对TEMT的负性调节作用可能是通过减少CTGF表达来实现的。     

RNA干扰沉默转化生长因子β1对大鼠移植肾上皮细胞转分化的作用

目的 探讨转化生长因子β1(TGF-β1)的RNA干扰质粒(shRNA-TGF-β1)对大鼠移植肾上皮细胞转分化的作用.方法 将受体大鼠分为4组:假手术组、质粒组、空质粒组、单纯移植组;建立大鼠肾移植纤维化加快模型,以流体力学为基础的肾脏基因转染方法转染质粒;分1、2、3个月收集肾脏标本;逆转录-PCR及Western印迹法检测TGF-β1、上皮钙黏蛋白(E-cadherin)的mRNA及蛋白表达;HE染色观察肾脏的病理变化;上皮钙黏蛋白及α肌动蛋白(α-SMA)免疫组化标记肾小管上皮细胞及成纤维母细胞.结果 质粒组TGF-β1在3个检测时间段表达均受到抑制,明显低于空质粒组、单纯移植组[第3个月时TGF-β1 mRNA分别为0.73±0.08比0.92±0.07、0.95±0.04(均P<0.01)];质粒组上皮钙黏蛋白表达高于空质粒组、单纯移植组[第3个月时上皮钙黏蛋白mRNA分别为0.39±0.11比0.15±0.07、0.17±0.06(均P<0.01);上皮钙黏蛋白蛋白分别为0.38±0.08比0.15±0.07、0.15±0.07(均P<0.01)];质粒组肾小管上皮细胞多于空质粒组、单纯移植组,成纤维母细胞少于空质粒组、单纯移植组;病理染色显示质粒组慢性炎症较轻,纤维母细胞较少,细胞外基质沉积较轻.结论 质粒shRNA-TGF-β1能抑制移植肾肾小管上皮细胞转分化,其机制可能是抑制TGF-β1的表达、上调上皮钙黏蛋白的表达.     

针对CTGF的siRNA对高糖诱导人肾小管上皮细胞转分化的影响

目的 探讨针对结缔组织生长因子（CTGF）基因的siRNA对高糖条件培养下的人肾小管上皮细胞株HK-2细胞向间充质细胞转分化（EMT）的影响。方法 将体外培养的HK-2细胞分为6组：(1)正常对照组,培养基含D-葡萄糖1g/L;(2)等渗对照组,培养基含D-葡萄糖1g/L,甘露醇3.5g/L;(3)高糖组组,培养基含D-葡萄糖4.5g/L;(4) 高糖+空白对照组：细胞转染空白质粒后于高糖培养基中培养;(5) 高糖+阴性对照组：细胞转染含无关序列的重组质粒后于高糖培养基中培养;(6) 高糖+干扰组：细胞转染针对CTGF的 siRNA表达质粒后于高糖培养基中培养。应用RT-PCR检测CTGF mRNA水平,Western blot法检测CTGF、E-钙黏蛋白（E-cadherin）、α-平滑肌肌动蛋白（α-SMA）蛋白水平。结果 高糖刺激可上调HK-2细胞的CTGF mRNA及蛋白水平,并呈时间依赖性降低HK-2细胞的E-cadherin蛋白表达水平,升高α-SMA蛋白表达水平。而通过特异性siRNA抑制CTGF mRNA表达后,细胞的CTGF蛋白表达随时间延长进行性降低,同时伴有细胞E-cadherin蛋白表达升高,α-SMA蛋白表达水平降低。结论 证实了CTGF是高糖诱导HK-2细胞EMT的重要介质。针对CTGF的siRNA能明显改善高糖诱导的肾小管上皮细胞EMT,为进一步寻找DN防治的靶点提供了新的实验依据。     

siRNA沉默CTGF表达对高糖诱导人肾小管上皮细胞转分化的影响

目的探讨针对结缔组织生长因子(CTGF)基因的siRNA对高糖条件培养下的人肾小管上皮细胞株HK-2细胞向间充质细胞转分化(EMT)的影响。方法将体外培养的HK-2细胞分为6组:(1)正常对照组,培养基含D-葡萄糖1g/L;(2)等渗对照组,培养基含D-葡萄糖1g/L,甘露醇3.5 g/L;(3)高糖组,培养基含D-葡萄糖4.5 g/L;(4)高糖+空白对照组:细胞转染空白质粒后于高糖培养基中培养;(5)高糖+阴性对照组:细胞转染含无关序列的重组质粒后于高糖培养基中培养;(6)高糖+干扰组:细胞转染针对CTGF的siR-NA表达质粒后于高糖培养基中培养。应用RT-PCR检测CTGF mRNA水平,Western blot法检测CTGF、E-钙黏蛋白(E-cadherin)、α-平滑肌肌动蛋白(α-SMA)蛋白水平。结果高糖刺激可上调HK-2细胞的CTGFmRNA及蛋白水平,并呈时间依赖性降低HK-2细胞的E-cadherin蛋白表达水平,升高α-SMA蛋白表达水平。而通过特异性siRNA抑制CTGF mRNA表达后,细胞的CTGF蛋白表达随时间延长进行性降低,同时伴有细胞E-cadherin蛋白表达升高,α-...     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Electro-Acupuncture Combined with the Trigger Point Needle-Embedding for Treatment of Primary Trigeminal Neuralgia in 31 Cases

In recent years, the author of this essay has applied electro-acupuncture combined with the trigger point needle-embedding for treatment of primary trigeminal neuralgia in 31 cases, yielding satis- factory results as reported in the following.     

Investigation of Matrix Metalloproteinase -1, 2 and tissue inhibitor of matrix metalloproteinases-1 in Endometriosis

Objective： To explore the role of matrix metalloproteinase-1,2 （MMP-1, MMP-2） and tissue inhibitor of matrix metalloproteinases-1 （TIMP-1） in endometriosis. Methods： The eutopic and ectopic endometria from 40 subjects suffering from endometriosis and regular.endometria from 40 subjects （excluding endometriosis） were collected and examined by in situ hybridization technology and western blot assay. Results： Both expressions of MMP-1 and -2 were stronger in ectopic endometrium and eutopic endometrium than in normal endometrium. On the contrary, the expression of TIMP-1 in ectopic endometrium and eutopic endometrium was lower. The differences were significant （P 〈 0.01 ）. Moreover, there was no relationship among the expressions of MMP-1, 2 and TIMP-1 in ectopic endometrium. Conclusion： The expressions of MMP-1, 2 and TIMP-1 lose balance and lack of periodic changes in ectopic endometrium , which explains the biological invasive behavior of endometriosis. It was suggested-that regulating the balance between the MMPs and TIMP-1 should be an ideal therapeutic target to endometriosis.     

Shi Da-zhuo (史大卓) —An outstanding cardiologist of integrative medicine

Prof. SHI Da-zhuo, Ph.D., male, was born on March 20, 1960. Prof. SHI entered the Ph.D. program in 1990 at the China Academy of Chinese Medical Sciences under the supervision of Prof. CHEN Ke-ji, majoring in the treatment of cardiovascular diseases. After receiving his Ph.D. degree in 1993, Prof. SHI started working at the Cardiovascular Center in Xiyuan Hospital affiliated to China Academy of Chinese Medical sciences.     

Persist in and Carry Forward the Integrative Medical Research——Speech at the 6th National General Congress of Chinese Association of Integrative Medicine by Academician CHEN Zhu,Minister of Ministry of Health

The 6th National General Congress of Chinese Association of Integrative Medicine （CALM） was convened at 19-20, April 2008 in Beijing. Academician CHEN Zhu, the minister of Ministry of Health indicated at the congress that the integration of Chinese and Western medicine is very well in keeping with the situation of our country and the general rule of development in medical science; and as a good integration of Chinese medicine and Western medicine, it is mutually beneficial and advantageous to both of them. Seeing the creativity shown in integrative medical investigation in theoretic and methodological sides, we should and must persist in and develop it.