紫外分光光度法测定复方替硝唑凝胶中替硝唑和盐酸利多卡因的含量

目的:建立紫外分光光度法测定复方替硝唑凝胶中替硝唑和盐酸利多卡因含量的方法.方法:分别采用单波长法和双波长法不经分离直接测定替硝唑和盐酸利多卡因的含量,替硝唑的测定波长为317 nm,盐酸利多卡因的测定波长为263 nm,参比波长为360 nm.结果:替硝唑在浓度为6～16 μg·ml-1范围内线性关系良好,r=0.999 8,平均回收率为101.0%,RSD为0.9%(n=9).盐酸利多卡因在浓度为0.100~0.501 mg·ml-1范围内线性关系良好,r=0.999 9,平均回收率为100.9%,RSD为0.6%(n=9).结论:该法操作简便,准确性较好,可用于直接测定复方替硝唑凝胶中替硝唑和盐酸利多卡因的含量.     

氯强油搽剂的制备与含量测定

目的制备氯强油搽剂,并用差式分光光度法测定氯强油搽剂中氯霉素和泼尼松含量.方法分别选择238 nm为泼尼松的测定波长,327 nm为参比波长,在292 nm处直接测定氯霉素的吸光度.结果氯霉素在5～16 μg·ml-1、泼尼松在0.3～0.9μg·ml-1范围内吸光度与浓度均呈良好的线性关系(r=0.999 5,r=0.999 4),两种组分的平均回收率和RSD值分别为99.74%、0.83%和99.59%、1.45%.结论用差式分光光度法测定氯强油搽剂中的泼尼松和氯霉素含量,方法简便准确,样品无需分离即可直接测定.     

双波长倍率系数法和一阶导数光谱法测定复方苯甲酸酊的含量

目的:建立复方苯甲酸酊中苯甲酸和水杨酸的含量测定方法。方法:采用倍率系数法于(272±1)nm和(301±1)nm波长处直接测定苯甲酸含量;采用一阶导数光谱法于(318±1)nm波长处直接测定水杨酸的含量。结果:苯甲酸:在19.34～27.08μg/mL浓度范围内,线性关系良好,相关系数r=0.999 9,平均回收率99.4％,RSD为0.80％。水杨酸:在24.48～34.27μg/mL浓度范围内,线性关系良好,相关系数r=0.999 9,平均回收率99.6％,RSD为0.490A。结论:本方法简便、快速、准确。     

荧光分光光度法测定利多卡因丁卡因注射液中盐酸丁卡因的含量

目的:建立荧光分光光度法测定利多卡因丁卡因注射液中盐酸丁卡因的含量.方法:以311nm为激发波长,370 nm为发射波长,测定相对荧强度.结果:盐酸丁卡因在0.4～1.2μg·ml-1浓度范围与相对荧光强度呈良好线性关系,r=0.999 8.平均回收率为100.1%,RSD0.8%.结论:本品专属性强,可用于该制剂中盐酸丁卡因的含量测定.     

紫外分光光度法测定水杨酸软膏中水杨酸含量

目的:建立水杨酸软膏的含量测定方法.方法:采用紫外分光光度法在波长296 nm处测定.结果:水杨酸在10～30μg/mL浓度范围内与吸收度呈良好的线性关系(r=0.999 9),平均回收率为99.08%,RSD=0.306%(n=9).结论:紫外分光光度法快速、简便、准确,适合于基层医院制剂的质量控制.     

紫外分光光度法测定注射用阿魏酸钠中间体的含量

目的:建立紫外分光光度法测定注射用阿魏酸钠中间体的含量.方法:紫外分光光度法,检测波长310nm.结果:阿魏酸钠在6.0～14.0μg·ml的范围内有良好的线性关系,(r=0.999 9),回收率99.64%,RSD=0.26%.结论:本方法简单快捷,结果准确.     

紫外分光光度法测定碘化钾溶液的含量

目的:建立紫外分光光度法测定碘化钾溶液中碘化钾含量。方法:采用紫外分光光度法在波长(226±1)nm处直接测定碘化钾含量。结果:碘化钾在4～12 μg·ml-1浓度范围内与吸收度的线性关系良好(r=0.999 9),平均回收率为100.5%,RSD为0.7%(n=6)。结论:本法简便、快速、准确,适用于碘化钾溶液的含量测定。     

醋酸地塞米松的含量测定

目的:建立测定复方醋酸地塞米松搽剂中醋酸地塞米松含量的方法.方法:采用紫外分光光度法,检测波长为243nm.结果:醋酸地塞米松在6～30μg·ml-1浓度范围内吸收度与浓度呈良好的线性关系(r=0.999 8),平均回收率为102.18%.结论:本方法操作简便,精密度好,结果准确可靠.     

安乃近片含量测定方法的探讨

目的:探讨安乃近片的含量测定方法.方法:采用紫外分光光度法测定安乃近片中安乃近的含量,测定波长为(257±1)nm.结果:安乃近在10～30μg·ml-1的浓度范围内,吸收度A与浓度C呈良好的线形关系(r=0.999 9,n=5),平均回收率为100.01%,RSD为0.91%(n=5).结论:紫外分光光度法干扰因素少,准确可靠,可用于安乃近片的含量测定.     

系数倍率法测定头液中水杨酸和苯酚的含量

目的测定头液中水杨酸和苯酚的含量。方法应用系数倍率紫外分光光度法,在298nm和272nm不经分离直接测定头液中两组份的含量。结果水杨酸在8~16μg.ml-1范围内与吸光度的线性关系良好(r=0.9999),平均回收率为99.71%,RSD=0.48%(n=3);苯酚在4~8μg.ml-1范围内与吸光度的线性关系良好(r=0.9998),平均回收率为99.33%,RSD=0.58%(n=3)。结论方法简便、快速、准确,适用于该制剂的快速检验。     

Comparison of the choleretic properties of bile acids

The choleretic properties of cholic, chenodeoxycholic, and deoxycholic acid and their taurine and glycine conjugates were compared to their ability to form micelles. It has previously been concluded that deoxycholate has the lowest critical micellar concentration; chenodeoxycholate is slightly higher and cholic is much higher. Conjugation with glycine and taurine has little or no effect on the critical micelle concentration. Since the choleretic properties of bile salts are thought to be directly proportional to their osmotic activities, one might suspect that deoxycholic acid would be the least choleretic, chenodeoxycholic slightly more choleretic and cholic much more choleretic, with little difference between the conjugated and unconjugated forms. However, in the present study, cholic, chenodeoxycholic and taurocholic acid produced similar increases in bile flow (450–700 μl/kg) after an equimolar dose (55 μM/kg). Except for the conjugation of deoxycholic acid with taurine, conjugation of these bile acids with glycine or taurine always decreased the choleretic properties of the bile acids. Therefore, it has been concluded that there is not a good correlation between the in vitro osmotic properties of bile acids and their ability to increase bile flow.     

抗癌活性成分满五酸等三个化合物的分离和鉴定

从产于四川宜宾翼梗五味子果实中,分得抗癌活性成分满五酸(1),同时分得schizandronic acid(2)和cuparenic acid(3),结构由与标准化合物比较光谱数据和物理常数而确证。     

Pomegranate (<Emphasis Type="Italic">Punica granatum</Emphasis>) pure chemicals show possible synergistic inhibition of human PC-3 prostate cancer cell invasion across Matrigel™

Summary Four pure chemicals, ellagic acid (E), caffeic acid (C), luteolin (L) and punicic acid (P), all important components of the aqueous compartments or oily compartment of pomegranate fruit (Punica granatum), and each belonging to different representative chemical classes and showing known anticancer activities, were tested as potential inhibitors of in vitro invasion of human PC-3 prostate cancer cells in an assay employing Matrigel™ artificial membranes. All compounds significantly inhibited invasion when employed individually. When C, P, and L were equally combined at the same gross dosage (4 μ g/ml) as when the compounds were tested individually, a supradditive inhibition of invasion was observed, measured by the Kruskal-Wallis non-parametric test.An erratum to this article can be found at     

HPLC法测定熊去氧胆酸片有关物质的含量

[摘要]目的：建立高效液相色谱法测定熊去氧胆酸片有关物质含量的方法。方法：用十八烷基硅烷键合硅胶为填充剂，以磷酸盐缓冲液（pH3.0）-甲醇-乙腈（35:37:28）为流动相，示差折光检测器测定。结果：在选定的色谱条件下，各成分可达到很好的分离，7-酮基胆石酸、胆酸、鹅去氧胆酸、胆石酸分别在0.05~2.00，0.05~2.00，0.05~2.00，0.10~2.00mg·mL-1的范围内线性关系良好(r>0.9989)；精密度、稳定性试验的RSD均不大于0.5%；回收率分别为100.1%、99.8%、100.7%和101.9%（n=9,RSD<1.4%）。7-酮基胆石酸、胆酸、鹅去氧胆酸、胆石酸定量限分别为3.0、2.5、7.0、20μg·mL-1，检测限分别为0.8、0.7、2.0、3.5μg·mL-1。测定供试品两批，供试品一7-酮基胆石酸检出量为0.03%，胆酸和胆石酸均未检出，鹅去氧胆酸检出量为0.66%；供试品二7-酮基胆石酸、鹅去氧胆酸检出量均为0.10%，胆酸和胆石酸均未检出。结论：该方法灵敏度高，专属性强，适于测定熊去氧胆酸片中有关物质的含量。     

Antitumor triterpenes from medicinal plants

Thirteen kinds of naturally occurring or derivatised triterpenes, reported to have an antitumoral property, were reinvestigated on the basis of their direct cytotoxicity or the inhibitory activity on cell growth against five kinds of cultured human tumor cells,i. e., A-549, SK-OV-3, SK-MEL-2, XF498 and HCT15,in vitro. Ursonic acidIII, betulinic acidVIII, betulonic acidX and glycyrrhetinic acidXI were exhibited a marked inhibition on cell growth.     

HPLC同时测定升麻中三个酚酸类成分的含量

目的建立快速测定升麻中咖啡酸、阿魏酸及异阿魏酸含量的HPLC方法。方法色谱柱为C18柱(4.6 mm×250 mm,5μm);流动相:乙腈-0.01%磷酸梯度洗脱;柱温:30℃;检测波长:320 nm;流速:1.0 ml/min。结果咖啡酸、阿魏酸、异阿魏酸分别在5.78~115.6μg/ml(r=0.9998,n=6),4.03~80.60μg/ml(r=1,n=6),16.45~329.00μg/ml(r=1,n=6)范围内线性关系良好,回收率在97.6%~101.5%。结论建立的方法准确可靠,可以用于测定升麻中咖啡酸、阿魏酸、异阿魏酸的含量。     

HPLC测定L-天门冬氨酸中的有关物质

目的建立L-天门冬氨酸中羟基丁二酸、丁二酸和富马酸等有关物质的检查方法。方法采用HPLC法,使用AQ C18色谱柱,0.01 mol.L-1的磷酸二氢钾水溶液(磷酸调pH3)为流动相,检测波长200 nm。结果主成分和杂质可完全分离。结论建立的方法灵敏、专属,可用于L-天门冬氨酸中有关物质的测定。     

Oral antipyretic therapy: Evaluation of the N-aryl-anthranilic acid derivatives mefenamic acid,tolfenamic acid and flufenamic acid

Summary The antipyretic activity of three N-aryl-anthranilic acid derivatives, mefenamic acid, tolfenamic acid and flufenamic acid, was compared and their optimal antipyretic dose determined in a trial in 87 children (aged 5 months to 15 years), who suffered from infections and fever exceeding 38.5°C. Tolfenamic acid proved to be the most potent antipyretic agent of the three drugs; it was eight times more powerful than mefenamic acid and three times more powerful than flufenamic acid. The optimal antipyretic doses were: mefenamic acid 4 mg/kg, tolfenamic acid 0.5 mg/kg and flufenamic acid 1.5 mg/kg. It is evident that the antipyretic activity of these anthranilic acid derivatives is even greater than their antirheumatic effect, the difference being most noticeable in the case of tolfenamic acid.