降钙素基因相关肽和心房肽对心肌缺血犬冠脉循环及离体冠脉…

本实验利用冠状内给药和离体血管灌流等方法，观察并比较了降钙素基因相关肽和心房肽对犬冠状脉循环的影响及心肌缺血后冠脉对二者反应性的变化。结果表明，冠脉内注射ＣＧＲＰ或ＡＮＰ均能明显逆转急性心肌缺血所致冠脉血流量的显著减少和大小冠脉阻力的显著增加，其中对远端小冠脉阻力降低的程度明显大于冠脉，而且ＣＧＲＰ的上述作用显著强于ＡＰ     

降钙素基因相关肽对大鼠心肌缺血损伤的影响

目的:观察降钙素基因相关肽对大鼠缺血心肌的保护作用。方法:用单剂量异丙肾上腺素皮下注射复制大鼠心肌缺血损伤模型,单剂量降钙素基因相关肽静脉注射治疗,2 h后采血测定血清心肌酶及血清MDA、SOD水平,同时取心肌组织匀浆测组织MDA、SOD水平,并观察心肌组织结构改变。结果:(1)异丙肾上腺素致缺血心肌损伤大鼠血清和心肌组织MDA升高、SOD下降,而CGRP治疗能逆转上述改变(P＜0.05)。(2)CGRP治疗组心肌酶CK、LDH明显低于损伤组(P＜0.05)。(3)心肌光镜、电镜结果示CGRP组细胞损伤程度明显低于损伤组(P＜0.05)。结论:CGRP对异丙肾上腺素所致大鼠缺血心肌有保护作用,抗膜脂质过氧化可能是其重要机制。     

降钙素/降钙素基因相关肽基因前体RNA的加工

降钙素和降钙素基因相关肽是由一个基因表达出的两种功能完全不同的多肽激素,其基因的表达主要在RNA水平上受到调控。前体RNA在加工成为成熟RNA的过程中,在不同的组织中经过不同的剪接形成不同的RNA。其剪接的特异性受到顺式元件和反式因子的调控,基因 的exon4上游剪接受点和下游polyA位点均存在弱的加工位点,通过增强子序列使之产生选择性剪接,另外,可能存在组织特异的反式剪接因子。     

降钙素基因相关肽对内皮素释放的影响

本实验在大鼠的整体及离体血管条上观察了降钙素基因相关肽（ＣＧＲＰ）对内皮素（ＥＴ）释放的影响。结果表明：ＣＧＲＰ静脉注射（５μｇ／ｋｇ）能明显降低大鼠内毒素血症时血浆ＥＴ含量，ＣＧＲＰ孵育（１０＾－８ｍｏｌ／Ｌ）离体的大鼠主动脉血管条能有效地抑制凝血酶（ｔｈｒｏｍｂｉｎ）引起的ＥＴ释放，但是ＣＧＲＰ静注及孵育不影响血浆ＥＴ的基础含量及离体血管条ＥＴ的基础释放。结果提示ＣＧＲＰ能抑制病理条件下ＥＴ的     

强利尿心钠素对实验犬冠脉循环及心肌氧代谢的影响

本文利用结扎犬冠脉左前降支造成急性缺血模型，观察了冠脉内灌注强利尿心钠素０．６μｇ．ｋｇ＾－１．ｍｉｎ＾－１，０．２μｇ．ｋｇ＾－１．ｍｉｎ＾－１及生理盐水对冠脉侧支循环及心肌氧代谢的影响。     

降钙素基因相关肽在部分消化系统疾病中的意义

我们测定了胃溃疡、十二指肠溃疡、肝硬化等消化系统疾病患者血浆降钙素基因相关肽(cGRP)的 水平,并对其结果进行了初步探讨,现报告如下。 材料和方法 一、对象:选择诊断明确的典型住院患者作为试验组,其中胃溃疡23例(男19,女4),年龄为33～59岁,平均46岁;十二指肠溃疡35例(男28,女17),年龄为25～48岁,平均37岁;肝硬化26例(男23,女3),年龄41～65岁,平均53岁。 二、样品采集:患者于清晨静脉取血3ml,注入含10％EDTA-Na_2100μl、抑肽酶40μl的试管中,混匀后于4℃3000rpm离心10min分离血浆,于-20℃备测。 三、方法:采用东亚免疫技术研究所提供的cGRP试剂盒。各组数据用t检验进行显著性测验。     

降钙素基因相关肽对脑缺血大鼠脑血流的影响

大鼠侧脑室注入降钙素基因相关肽（ＣＧＲＰ）或等体积甘露醇为对照，用非损伤阻抗法测定一侧颈总动脉结扎后ＣＧＲＰ对脑血流的影响。结果表明，一侧颈总动脉结扎后，脑血流量降低３４％，此时侧脑室给予ＣＧＲＰ即刻使脑血流图波幅增加，５分钟时达高峰，比注药前平均增加６５％，与甘露醇对照相比差别非常显著（Ｐ＜０．０１），４０分钟时作用开始减弱，１００分钟后作用消失。与颈总动脉结扎前相比，在脑血供不足时，ＣＧＲＰ增     

降钙素基因相关肽对定量冠脉狭窄后冠脉血流动力学的作用

本实验用犬冠脉定量狭窄模型，观察了由冠脉注射降钙素基因相关肽对犬正常有不同程度冠脉狭窄时的冠脉血流动力学的影响，结果表明，冠脉内注射ＣＧＲＰ（０．３ｇ／ｋｇ）可使冠脉血流量和远端小动脉压有不同程度增加，平均动脉压轻度下降，冠脉血管总阻力、大冠脉血管阻力及小冠脉血管阻力减小。在中度冠脉狭窄３０ｍｉｎ后注射ＣＧＲＰ，ＭＡＰ无明显改变，ＣＢＦ增高，而ＤＣＰ、ＲＴ、ＲＬ、ＲＳ均降低，且持续３０ｍｉｎ，在重     

降钙素基因相关肽对大鼠血糖及血糖调节激素的影响

基于外周给与CGRP能使血糖升高,胰岛素分泌下降,本实验目的在于研究CCRP是否参与血糖浓度的调节及其可能机理。结果表明:静脉灌注CGRP在常态下能使血糖升高,而不影响胰岛素的浓度,在高血糖刺激下CGRP具抑制胰岛素分泌的功能;静脉灌注CGRP对糖耐量曲线有明显影响;脑室注射CGRP可使血糖明显降低。本实验提示CGRP有可能与糖尿病的发生发展有一定关系。     

清醒心肌缺血模型的可控冠状动脉狭窄程度

BACKGROUND:Anesthesia is a protective measure to animals in animal experiments, but the use of the corresponding anesthetic drugs will have different effects on the experimental results. OBJECTIVE:To analyze the differences of various indexes and the therapeutic effect of myocardial ischemia canine model of coronary stenosis under the condition of consciousness and anesthesia. METHODS:Twenty Beagle canine myocardial ischemia models were equally assigned to five groups, and prepared according to the complete orthogonal Latin square arrangement for the experiment. Intervention procedures included the normal observation (normal control for 10 minutes, observation for 30 minues), conscious model (after normal control, coronary artery stenosis for 10 minutes, and observation for 30 minutes), conscious treatment (after normal control, coronary artery stenosis for 10 minutes, 50 μg/kg nitroglycerin sublingually buccal, and observation for 30 minutes), anesthesia model (animals received anesthesia; after normal control, coronary artery stenosis for 10 minutes, and observation for 30 minutes), anesthesia treatment (animals received anesthesia; after normal control, coronary artery stenosis for 10 minutes, 50 μg/kg nitroglycerin sublingually buccal, and observation for 30 minutes). We recorded canine electrocardiogram, blood pressure, heart rate and body temperature level when animals were conscious after coronary artery stenosis, and mean arterial blood pressure, body temperature, heart rate, electrocardiogram ST segment changes in conscious and anesthetic states in myocardial ischemia dogs, and observed the therapeutic effect of nitroglycerin on conscious and anesthetic states in myocardial ischemia dogs. RESULTS AND CONCLUSION:The electrocardiogram, blood pressure, heart rate and body temperature were changed after the coronary artery stenosis in conscious dogs, and the ST segment of electrocardiogram was significantly increased. The blood pressure and heart rate were significantly higher than that in the conscious state (P < 0.05), and the ST segment of electrocardiogram in dogs was significantly increased in conscious and anesthetic states. After the treatment of nitroglycerin, the effect was obvious in the myocardial ischemia model in conscious state. The results showed that the model of myocardial ischemia and the clinical fitting degree were higher, which could reduce the effect of anesthesia on the experimental results.     

在体心肌缺血与胰岛素对犬心肌细胞GLUT移位和葡萄糖摄取的相加作用

目的：观察在体犬心肌缺血时输入胰岛素能否对GLUT1和GLUT4移位和葡萄糖摄取有相加作用。方法：用自动分析仪测定生理代谢参数,用多普勒测定心肌收缩功能,用免疫法检测GLUT。结果：生理条件下胰岛素使心肌细胞膜GLUT4增加2倍,GLUT1增加1.5倍,同时心肌葡萄糖摄取增加3倍。急性心肌缺血时输入胰岛素,使缺血区冠状动-静脉葡萄糖浓度相差近4倍,缺血区细胞膜GLUT1和GLUT4明显增加。结论：胰岛素引起GLUT1和GLUT4移位,使心肌葡萄糖摄取增加。心肌缺血+胰岛素对心肌GLUT1和GLUT4移位和葡萄糖摄取有相加作用。提示在心肌缺血时,应用胰岛素有助于增加心肌葡萄糖的摄取和利用。     

Cardiovascular reactivity and mental stress-induced myocardial ischemia in patients with coronary artery disease

This report evaluates the relationships of hemodynamic reactivity and determinants of myocardial oxygen demand to myocardial ischemia during mental stress in coronary artery disease patients. Thirty-nine patients and 12 controls were studied by radionuclide ventriculography during three mental tasks (arithmetic, Stroop task, and simulated public speaking). Patients were subdivided into three groups based on the severity of ischemic wall motion responses to the mental stressors. Results revealed that systolic blood pressure (SBP) levels during the mental tasks and SBP reactivity (increases) to stress were highest for the severely ischemic group, lowest for controls, with the mild-moderate ischemic and nonischemic patients in between. Severely ischemic patients started out with lower double product (heart rate x SBP) levels, and reached higher levels during the Stroop and speech tasks. There were no reliable group effects for diastolic blood pressure, heart rate, or left ventricular end-diastolic volumes. Among severely ischemic patients, the most potent task in eliciting ischemia (the speech) was associated with higher cardiovascular levels and elicited greater heart rate, double product, and ventricular volume responses. The present data indicate a relationship between cardiovascular levels and reactivity and the magnitude of ischemia induced by mental stress.     

金属硫蛋白在心肌缺血再灌注损伤修复中的抗氧化作用

目的 探讨金属硫蛋白（ＭＴ）在心肌梗塞和缺血再灌注心肌损伤修复中与脂质过氧化的关系。方法 采用大鼠心肌梗塞／缺血再灌注模型,测定手术后２４ｈ和８周心肌组织的ＭＴ及脂质过氧化的中间产物丙二醛（ＭＤＡ）的含量,结果：心肌梗塞和缺血再灌注２４ｈ后ＭＤＡ含量均显著高于对照组（Ｐ〈０．０１）,８周后ＭＤＡ显著降低（Ｐ〈０．０５）,而ＭＴ的含量在术后２４ｈ即升高,８周ＭＴ继续升高,缺血再灌注组ＭＴ达（３１４．