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1.
Wojciech Jelski Miroslaw Kozlowski Jerzy Laudanski Jacek Niklinski Maciej Szmitkowski 《Clinical and experimental medicine》2009,9(2):131-137
Various alcohol dehydrogenase (ADH) isoenzymes and aldehyde dehydrogenase (ALDH) exist in the human esophageal mucosa. In
our last experiments we have shown that ADH and ALDH are present also in the esophageal cancer cells. Moreover, the activities
of total ADH and class IV isoenzymes were significantly higher in cancer tissue than in healthy mucosa, which suggests that
these changes may be reflected by enzyme activity in the serum. Therefore, we measured the activity of total alcohol dehydrogenase,
and classes I–IV of this enzyme and aldehyde dehydrogenase in the sera of patients with this cancer. Serum samples were taken
for routine biochemical investigation from 67 patients with esophageal cancer before treatment. Total ADH activity was measured
by photometric method with p-nitrosodimethylaniline (NDMA) as a substrate and ALDH activity by the fluorometric method with 6-methoxy-2-naphtaldehyde
as a substrate. For the measurement of the activity of class I and II isoenzymes, we employed the fluorometric methods, with
class-specific fluorogenic substrates. The activity of class III alcohol dehydrogenase was measured by the photometric method
with formaldehyde and class IV with m-nitrobenzaldehyde as a substrate. A statistically significant increase of class IV alcohol dehydrogenase isoenzymes was found
in the sera of cancer patients. The median activity of this class isoenzyme in the total cancer group increased by about 26.5%
(7.42 mU/l) in comparison to the control level (5.46 mU/l). The total alcohol dehydrogenase activity was significantly higher
(30%) among patients with cancer. The activities of other tested ADH isoenzymes and total ALDH were unchanged. The activity
of the class I ADH isoenzyme was significantly higher in the sera of drinkers with esophageal cancer than non-drinking patients.
The increased total activity of alcohol dehydrogenase and class IV isoenzyme in the sera of patients with esophageal cancer
probably can be caused by release of this isoenzyme from cancer cells or might be stimulated by alcohol drinking. 相似文献
2.
Jelski W Kutylowska E Laniewska-Dunaj M Orywal K Laszewicz W Szmitkowski M 《Experimental and molecular pathology》2011,(2):631-635
Objective
Acute and chronic pancreatitis is a major complication of alcohol abuse. The pancreas can metabolize ethanol via oxidative pathway involving the enzymes — alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) as well as the nonoxidative pathway. Human pancreas tissue contains various ADH isoenzymes and possesses also ALDH activity. In this paper we have measured the activity of alcohol dehydrogenase isoenzymes, and aldehyde dehydrogenase in the sera of patients with acute and chronic pancreatitis.Methods
Serum samples were taken for routine biochemical investigation from 46 patients suffering from acute pancreatitis and 32 patients with chronic pancreatitis. Total ADH activity was measured by photometric method with p-nitrosodimethylaniline (NDMA) as a substrate and ALDH activity by the fluorometric method with 6-methoxy-2-naphtaldehyde as a substrate. For the measurement of the activity of class I isoenzymes we employed the fluorometric methods, with class-specific fluorogenic substrates. The activity of class III alcohol dehydrogenase was measured by the photometric method with n-octanol and class IV with m-nitrobenzaldehyde as a substrate.Results
A statistically significant increase of class III alcohol dehydrogenase isoenzymes was found in the sera of patients with acute and chronic pancreatitis. The median activity of this class isoenzyme in the patients group increased about 35% in the comparison to the control level. The total alcohol dehydrogenase activity was also significantly higher (23.5%) among patients with pancreatitis than healthy ones. The activities of other tested ADH isoenzymes and total ALDH were unchanged. The activity of the class I ADH isoenzyme was significantly higher in the sera of heavy drinkers with pancreatitis.Conclusion
We can state that the increase of the activity of class III alcohol dehydrogenase isoenzyme in the sera of pancreatitis patients seems to be caused by the release of this isoenzyme from damaged pancreatic cells. 相似文献3.
Abstract Alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) play a significant role in the metabolism of many biological
substances. ADH participates in the metabolism of ethanol, retinoic acid, lipid peroxidation products, leukotriene and glutathione
metabolism. ALDH is responsible for oxidation of acetaldehyde and other aldehydes and metabolism of histamine and retinoic
acid. The aim of this study was to compare the metabolism in breast cancer cells and normal breast parenchyma by measuring
ADH isoenzymes and ALDH activities in these tissues. Total ADH activity was measured by a photometric method with p-nitrosodimethylaniline
(NDMA) as a substrate. For the measurement of the activity of ALDH and class I and II isoenzymes of ADH we employed the fluorometric
methods, with class-specific fluorogenic substrates. The activity of class III alcohol dehydrogenase was detected by the photometric
method with n-octanol and class IV with m-nitrobenzaldehyde as substrates. The samples were taken surgically during resection
of breast carcinoma from 75 women. The activity of the class I ADH isoenzyme was significantly lower in breast cancer cells
than in healthy tissues. The other tested classes of ADH had a tendency for higher levels of activity in cancer cells than
in normal mammary tissue. The activity of total ADH and ALDH was also not significantly lower in the cancer cells. The decrease
of activity of class I ADH isoenzyme in breast cancer tissues may be a factor of some disorders in metabolic pathways with
participation of these isoenzymes that can lead to carcinogenesis. 相似文献
4.
Wojciech Jelski Karolina Orywal Magdalena Laniewska Maciej Szmitkowski 《Clinical and experimental medicine》2010,10(4):215-219
Alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) are present in gastric cancer cells (GC). Moreover, the activity
of total ADH and class IV isoenzymes is significantly higher in cancer tissue than in healthy mucosa. The activity of these
enzymes in cancer cells is probably reflected in the sera and could thus be helpful for diagnostics of gastric cancer. The
aim of this study was to investigate a potential role of ADH and ALDH as tumor markers for gastric cancer. We defined diagnostic
sensitivity, specificity, predictive value for positive and negative results, and receiver-operating characteristics (ROC)
curve for tested enzymes. Serum samples were taken from 168 patients with gastric cancer before treatment and from 168 control
subjects. Total ADH activity and class III and IV isoenzymes were measured by photometric but ALDH activity and ADH I and
II by the fluorometric method, with class-specific fluorogenic substrates. There was significant increase in the activity
of ADH IV isoenzyme and ADH total in the sera of gastric cancer patients compared to the control. The diagnostic sensitivity
for ADH IV was 73%, specificity 79%, positive and negative predictive values were 81 and 72% respectively. Area under ROC
curve for ADH IV was 0.67. The results suggest a potential role for ADH IV as marker of gastric cancer. 相似文献
5.
Karolina Orywal Wojciech Jelski Tadeusz Werel Maciej Szmitkowski 《Advances in medical sciences》2018,63(1):1-4
Purpose
In a previous study we showed that the total activity of alcohol dehydrogenase (ADH) and its isoenzyme class I was significantly higher in renal cancer (RCC) cells compared to normal kidney. The aim of this study was to compare the activities of ADH isoenzymes and aldehyde dehydrogenase (ALDH) in the sera of patients with different stages of RCC and healthy subjects.Materials and methods
Serum samples were taken from 54 patients with clear cell RCC (17 patients in stage II, 22 in stage III and 15 in stage IV) and 52 healthy patients. Class III, IV of ADH and the total ADH activity was measured by the photometric method. For the measurement of ADH class I, II and the total ALDH activity we employed the fluorometric method.Results
The total activity of ADH and its isoenzyme class I were significantly higher in the sera of patients with every stage of RCC compared to healthy subjects. The analysis of ALDH activity did not indicate significant differences between tested groups.Conclusions
The increased activity of total ADH and its isoenzyme class I in the sera of patients with RCC, seems to be caused by isoenzymes being released from cancerous cells and may be useful for diagnostics of renal cancer. 相似文献6.
Wojciech Jelski Karolina Orywal Marek Gacko Maciej Szmitkowski 《Experimental and molecular pathology》2009,87(1):59-62
Objective
Human blood vessels contain a huge amount of alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH), which play a significant role in the metabolism of many biological substances and participate in various metabolic pathways. The aim of this study was the investigation of the differences between the activities of ADH and ALDH in the wall of aortic aneurysm and wall of healthy aorta, that can explain the pathological background of aneurysm development.Methods
For the measurement of the activity of class I and II ADH isoenzymes and ALDH activity the fluorometric methods was employed. The total ADH activity and activity of class III and IV isoenzymes was measured by the photometric method. The study material consisted of vessels wall samples obtained from 45 abdominal aortic aneurysm.Results
The activity of the class I ADH isoenzyme was significantly lower in the wall of aortic aneurysm than in healthy aorta. The other tested classes of ADH showed the tendency to lower level of the activity in aneurysm tissue than that in wall of unchanged aorta. The activities of total ADH and ALDH were also not significantly lower in the aneurysms.Conclusion
The decrease of the activity of class I ADH isoenzymes in the wall of aortic aneurysm may be a factor of some disorders in metabolic pathways with participation of these isoenzymes. 相似文献7.
Wojciech Jelski Magdalena Laniewska-Dunaj Anna Strumnik Maciej Szmitkowski 《Archives of Medical Science》2014,10(5):951-955
Introduction
Helicobacter pylori infection is associated with decreased alcohol dehydrogenase (ADH) activity in the gastric mucosa. The decrease in gastric ADH activity depends on the severity of inflammation and mucosal injury. This damage can be a reason of the release of enzyme from gastric mucosa and leads to the increase of the ADH activity in the sera of patients with H. pylori infection.Material and methods
Serum samples were taken from 140 patients with H. pylori infection. Total ADH activity was measured by photometric method with p-nitrosodimethylaniline as a substrate and ALDH activity by the fluorometric method with 6-methoxy-2-naphtaldehyde. For the measurement of the activity of class I and II isoenzymes we employed the fluorometric methods, with class-specific fluorogenic substrates. The activity of class III ADH was measured by the photometric method with n-octanol and class IV with m-nitrobenzaldehyde as a substrate.Results
The activity of ADH IV in the serum of patients with H. pylori infection increased about 42% (7.86 mU/l) in the comparison to the control level (4.52 mU/l). Total activity of ADH was 1105 mU/l in patients group and 682 mU/l in control. The diagnostic sensitivity for ADH IV was 88%, specificity 90%, positive and negative predictive values were 91% and 84% respectively. Area under ROC curve for ADH IV was 0.84.Conclusions
Helicobacter pylori infection of gastric mucosa is reflected in the serum by significant increase of class IV and total ADH activity. The results suggest a potential role for ADH IV as a marker of H. pylori infection. 相似文献8.
PurposeThe metabolism of cancerous cells is in many ways different than in healthy cells. In ovarian cancer, cells exhibit activity of alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH), which participate in metabolism of many biological substances. The aim of this study was to compare the metabolism of ovarian cancer cells, ovarian cysts and normal ovarian cells by measurement of ADH isoenzymes and ALDH activities.Material and MethodsThe study material consisted of 36 cancerous ovarian tissues. Class III, IV of ADH and total ADH activity was measured by the photometric method and class I, II ADH and ALDH activity by the fluorometric method with class-specific fluorogenic substrates.ResultsThe activity of the class I ADH isoenzyme and the total ADH was significantly higher in ovarian cancer as compared to ovarian cysts and healthy tissues but there are no significant differences between ovarian cysts and healthy cells. The other classes of ADH tested, did not show significant differences between activity of cancerous cells and healthy ovary.ConclusionThe increased activity of total ADH in ovarian cancer, especially the class I isoenzyme and normal activity of ALDH, may be the factor for the disturbances in important biological substances metabolism and could increase the concentration of highly carcinogenic acetaldehyde. 相似文献
9.
According to International Agency for Research on Cancer, ethanol and acetaldehyde belong to group 1 of human carcinogens. The accurate mechanism by which alcohol consumption enhances carcinogenesis is still unexplained. Alcohol is oxidized primarily by alcohol dehydrogenase (ADH) to acetaldehyde, a substance capable of initiating carcinogenesis by forming adducts with proteins and DNA and causing mutations. Next, acetaldehyde is metabolized by aldehyde dehydrogenase (ALDH) to acetate. In tissues of many cancers, we can observe significantly higher activity of total alcohol dehydrogenase with any change in aldehyde dehydrogenase activity in comparison with healthy cells. Moreover, in malignant diseases of digestive system, significantly increased activity of ADH isoenzymes class I, III and IV was found. The gynecological, brain and renal cancers exhibit increased activity of class I ADH. ADH and ALDH can play also a crucial regulatory role in initiation and progression of malignant diseases by participation in retinoic acid synthesis and elimination of toxic acetaldehyde. Besides, changes of enzymes activities in tumor cells are reflected in serum of cancer patients, which create the possibilities of application ADH isoenzymes as cancer markers. 相似文献
10.
Holly R. Thomasson David W. Crabb Howard J. Edenberg Ting-Kai Li 《Behavior genetics》1993,23(2):131-136
The alcohol-flush reaction occurs in Asians who inherit the mutantALDH2
*2 allele that produces an inactive aldehyde dehydrogenase enzyme. In these individuals, high blood acetaldehyde levels are believed to be the cause of the unpleasant symptoms that follow drinking. We measured the alcohol elimination rates and intensity of flushing in Chinese subjects in whom the alcohol dehydrogenaseADH2 andALDH2 genotypes were determined. We also correlatedADH2, ADH3, andALDH2 genotypes with drinking behavior in 100 Chinese men. We discovered thatADH2
*2 andADH3
*1, alleles that encode the high activity forms of alcohol dehydrogenase, as well as the mutantALDH2
*2 allele were less frequent in alcoholics than in controls. The presence ofALDH2
*2 was associated with slower alcohol metabolism and the most intense flushing. In those homozygous forALDH2
*1, the presence of twoADH2
*2 alleles correlated with slightly faster alcohol metabolism and more intense flushing, although a great deal of variability in the latter was noted. 相似文献
11.
Lech Chrostek Maciej Szmitkowski Wojciech Jelski 《Experimental and toxicologic pathology》2001,53(1):77-80
The effect of methanol poisoning of rats on the hepatic activities of enzymes metabolizing alcohols was evaluated. The activities of alcohol (ADH) and aldehyde dehydrogenase (ALDH) in the liver of rats dosed with 1.5 and 3 g of methanol/kg b.w. were measured with new fluorogenic substrates (4-methoxy-1-naphthaldehyde [MONAL-41] for ADH and 6-methoxy-2-naphthaldehyde [MONAL-62] for ADH and ALDH) after 6, 12 and 24 hours and 2, 5 and 7 days. The methanol intoxication led to a dose dependent induction of ADH and ALDH activities. The higher dose of methanol induced the activities measured with both MONAL-41 and MONAL-62 with the peak on day 5; its effect was largest on the activity of ADH measured with MONAL-41. Only ADH activity measured with this substrate was induced by the lower dose of methanol during the whole time of the experiment; the activity of ADH measured with MONAL-62 and that of ALDH were induced only on day 1 of the intoxication. It is evident that sublethal methanol intoxication induces the hepatic activities of ADH and ALDH measured with fluorogenic substrates, and this induction depends on the dose of this alcohol. 相似文献
12.
The ADHE family of enzymes are bifunctional acetaldehyde dehydrogenase (ALDH)/alcohol dehydrogenase (ADH) enzymes that probably arose from the fusion of genes encoding separate ALDH and ADH enzymes. Here we have used the Entamoeba histolytica alcohol dehydrogenase 2 (EhADH2) enzyme as a prototype to analyze the structure and function of the ALDH domain of ADHE enzymes. We find that the N-terminal domain of EhADH2, encompassing amino acids 1-446, is sufficient for ALDH activity, consistent with the concept that EhADH2, and other members of the ADHE family comprise fusion peptides. In addition, we show, using site directed mutagenesis, that the catalytic mechanism for the ALDH activity appears to be similar to that described for other members of the ALDH extended family. 相似文献
13.
目的: 检测大鼠心肌在缺氧条件下发生细胞凋亡的变化,以及乙醛脱氢酶2(ALDH2)在此过程中所起的作用。 方法: 运用缺氧模型,比较大鼠心肌细胞在单独缺氧和经ALDH2特异性抑制剂daidzin预处理24 h后缺氧的凋亡改变,酶活性检测采用乙醛代谢法、凋亡的测定通过用Hoechest 33324、免疫荧光标记用流式细胞仪测定和TUNEL试剂盒检测。 结果: 心肌细胞在daidzin作用下,其酶活性被抑制而细胞无凋亡发生。在daidzin预处理24 h后再经缺氧诱导,比单独缺氧引起的心肌细胞凋亡更为明显:表现为在Hoechest 33324染色中,细胞核溶解和核碎裂(P<0.05),FACS和TUNEL显示,凋亡细胞明显增加(P<0.05)。 结论: ALDH2酶活性降低可增加心肌细胞对缺氧导致凋亡的易感性,ALDH2对缺氧引起的细胞凋亡有拮抗作用。 相似文献
14.
Hui Li Svetlana Borinskaya Kimio Yoshimura Nina Kal'ina rey Marusin Vadim A. Stepanov Zhendong Qin Shagufta Khaliq Mi-Young Lee Yajun Yang Aisha Mohyuddin David Gurwitz Syed Qasim Mehdi Evgeny Rogaev Li Jin Nikolay K. Yankovsky Judith R. Kidd Kenneth K. Kidd 《Annals of human genetics》2009,73(3):335-345
Mitochondrial aldehyde dehydrogenase (ALDH2) is one of the most important enzymes in human alcohol metabolism. The oriental ALDH2*504Lys variant functions as a dominant negative, greatly reducing activity in heterozygotes and abolishing activity in homozygotes. This allele is associated with serious disorders such as alcohol liver disease, late onset Alzheimer disease, colorectal cancer, and esophageal cancer, and is best known for protection against alcoholism. Many hundreds of papers in various languages have been published on this variant, providing allele frequency data for many different populations. To develop a highly refined global geographic distribution of ALDH2*504Lys , we have collected new data on 4,091 individuals from 86 population samples and assembled published data on a total of 80,691 individuals from 366 population samples. The allele is essentially absent in all parts of the world except East Asia. The ALDH2*504Lys allele has its highest frequency in Southeast China, and occurs in most areas of China, Japan, Korea, Mongolia, and Indochina with frequencies gradually declining radially from Southeast China. As the indigenous populations in South China have much lower frequencies than the southern Han migrants from Central China, we conclude that ALDH2*504Lys was carried by Han Chinese as they spread throughout East Asia. Esophageal cancer, with its highest incidence in East Asia, may be associated with ALDH2*504Lys because of a toxic effect of increased acetaldehyde in the tissue where ingested ethanol has its highest concentration. While the distributions of esophageal cancer and ALDH2*504Lys do not precisely correlate, that does not disprove the hypothesis. In general the study of fine scale geographic distributions of ALDH2*504Lys and diseases may help in understanding the multiple relationships among genes, diseases, environments, and cultures. 相似文献
15.
There is accumulating evidence indicating that aldehyde dehydrogenase (ALDH) activity selects for cancer cells with increased aggressiveness, capacity for sustained proliferation, and plasticity in primary tumors. However, emerging data also suggests an important mechanistic role for the ALDH family of isoenzymes in the metastatic activity of tumor cells. Recent studies indicate that ALDH correlates with either increased or decreased metastatic capacity in a cellular context-dependent manner. Importantly, it appears that different ALDH isoforms support increased metastatic capacity in different tumor types. This review assesses the potential of ALDH as biological marker and mechanistic mediator of metastasis in solid tumors. In many malignancies, most notably in breast cancer, ALDH activity and expression appears to be a promising marker and potential therapeutic target for treating metastasis in the clinical setting. 相似文献
16.
Some guinea pig tissue homogenates have shown the ability to catabolize,in vitro, imidazoloacetaldehyde (ImAAL) and gamma-aminobutyraldehyde (GABAL) via NAD-dependent aldehyde dehydrogenase (ALDH, EC 1.2.1.3). The liver, kidney, small intestine and gastric mucosa are the richest sources of ALDH activity towards ImAAL. The liver, kidney, small intestine and pancreas also show ALDH activity with GABAL as a substrate. All tissues tested have shown low Km and high Km ALDH activity with propionaldehyde as substrate. The guinea pig liver ALDH which is able to oxidize ImAAL and GABAL is located exclusively in the cytoplasm. 相似文献
17.
18.
Ohi Y Umekita Y Yoshioka T Souda M Rai Y Sagara Y Sagara Y Sagara Y Tanimoto A 《Histopathology》2011,59(4):776-780
Ohi Y, Umekita Y, Yoshioka T, Souda M, Rai Y, Sagara Y, Sagara Y, Sagara Y & Tanimoto A(2011) Histopathology 59 , 776–780 Aldehyde dehydrogenase 1 expression predicts poor prognosis in triple‐negative breast cancer Aims: Aldehyde dehydrogenase 1 (ALDH1) has been identified as a reliable marker of breast cancer stem cells, and its clinical significance as a prognostic indicator of breast cancer has been reported by several investigators. However, the clinical significance of ALDH1 expression in triple‐negative (TN) breast cancer, a high‐risk breast cancer lacking the benefit of specific therapy, remains to be solved. Methods and results: We performed immunohistochemical analyses of 106 TN breast cancers, using paraffin‐embedded sections. The basal‐like phenotype was also investigated with the use of basal cytokeratin 5/6 and epidermal growth factor receptor. ALDH1 expression in carcinoma cells was found in 59% of cases and was correlated with high histological grade alone (P < 0.006), whereas ALDH1 expression in stromal cells was found in 49% of cases but was not correlated with any clinicopathological parameter. Patients with ALDH1 expression in carcinoma cells had a shorter relapse‐free survival (RFS) according to the log‐rank test (P = 0.015). According to Cox multivariate analysis, ALDH1 expression in carcinoma cells was an independent prognostic indicator of RFS (P = 0.025). The log‐rank test revealed that stromal expression of ALDH1 had no effect on RFS. Conclusions: ALDH1 expression in carcinoma cells is an independent prognostic factor in TN breast cancer patients. 相似文献
19.
Ziyuan Xu Zhengyu Shen Linjun Shi Hongying Sun Wei Liu Zengtong Zhou 《Annals of diagnostic pathology》2013,17(5):408-411
Oral lichen planus (OLP) is a potentially malignant disorder associated with an increased risk of oral squamous cell carcinoma (OSCC). The objective of this study was to determine protein expression of cancer stem cell marker aldehyde dehydrogenase 1 (ALDH1) in a series of patients with OLP and evaluate the correlation between ALDH1 expression and the risk of progression to OSCC. In a retrospective study, ALDH1 expression was determined using immunohistochemistry in samples from 101 patients with OLP who received a mean follow-up of 5 years, including 89 patients with untransformed OLP that did not develop into OSCC and 12 patients with malignant transformed OLP that had developed into OSCC. Analysis of 10 cases of normal oral mucosa and 6 cases of postmalignant OSCC form previously diagnosed OLP was also performed. The results showed that ALDH1 expression was observed in 27 (30.3%) of 89 cases of untransformed OLP and in 8 (66.7%) of 12 cases of transformed OLP (P = .021). Aldehyde dehydrogenase 1 was not expressed in normal oral mucosa, but it overexpressed in the 6 cases (100%) of OSCC. Multivariate analysis revealed that ALDH1 expression was significantly associated with a 6.71-fold (95% confidence interval, 1.64-27.42; P = .008) increased risk of malignant transformation. Collectively, ALDH1 expression was significantly associated with malignant transformation in a large series of patients with OLP. Our findings suggested that ALDH1 expression may identify a subgroup of a higher risk of malignant transformation of OLP. 相似文献
20.
Both alcohol dehydrogenase (ADH) and the two isoenzymes of aldehyde dehydrogenase (ALDH-I-NAD+ and ALDH-II-NAD+) were first detected in foetal rat liver about 5 days before birth. All enzymes developed gradually and showed no abrupt increases in activity. The specific activities of ALDH-I-NAD+ and ALDH-II-NAD+ in the mitochondrial fractions, ALDH-II-NAD+ in the microsomal fractions and ADH in liver homogenates all produced a major percentage of the adult activity within a month, whereas the total activities increased over a longer part of the developmental period. 相似文献