白花银背藤化学成分的研究

目的研究白花银背藤A rgy reia segu in ii的化学成分。方法采用硅胶和Sephadex LH-20柱色谱进行分离,光谱数据结合理化性质鉴定化合物的结构。结果从白花银背藤地上部分分离、鉴定了11个化合物,分别为:β-谷甾醇(Ⅰ)、豆甾醇(Ⅱ)、胡萝卜苷(Ⅲ)、东莨菪亭(Ⅳ)、邻羟基苯甲酸(Ⅴ)、3,4-二羟基苯甲醛(Ⅵ),N-反式-对羟基苯乙基香豆酰胺(N-trans-p-hydroxyphenethy l coum aram ine,Ⅶ)、N-反式-对羟基苯乙基阿魏酰胺(N-trans-p-hy-droxyphenethy l fero lam ine,Ⅷ)、3,5-二咖啡酰奎宁酸甲酯(3,5-d i-O-caffeoy lqu in ic ac id m ethy l ester,Ⅸ)、3,4-二咖啡酰奎宁酸甲酯(3,4-d i-O-caffeoy lqu in ic ac id m ethy l ester,Ⅹ)、4,5-二咖啡酰奎宁酸甲酯(4,5-d i-O-caffeoy lqu in icac id m ethy l ester,Ⅺ)。结论化合物Ⅰ~Ⅺ均为首次从本植物中分离得到。     

白鹤藤化学成分研究

目的 研究白鹤藤(Argyreia acuta Lour.)的化学成分.方法 采用硅胶柱色谱、Sephadex LH-20柱色谱、ODS柱色谱、MCI柱色谱、半自动制备液相色谱等手段进行分离纯化,通过理化性质和波谱解析鉴定化合物的结构.结果 从白鹤藤的乙酸乙酯部位和石油醚部位分离得到8个化合物,分别鉴定为3,4-0-二咖啡酰基奎宁酸甲酯(1)、3,4-0-二咖啡酰基奎宁酸乙酯(2)、3,5-0-二咖啡酰基奎宁酸丁酯(3)、3,4-O-二咖啡酰基奎宁酸丁酯(4)、木栓醇(5)、木栓酮(6)、菠甾醇(7)、豆甾醇(8).结论 化合物1～7首次从该属植物中分离得到.     

中药川续断化学成分的研究Ⅲ

[目的]为了研究中药川续断干燥根的化学成分。[方法]应用多种色谱手段,对川续断根50%乙醇大孔树脂洗脱部分进行系统的分离和纯化,并利用NMR和MS等方法解析化合物结构。[结果]共得到6个化合物,分别鉴定为:1)(+)-1-羟基松脂醇4'-O-β-D-吡喃葡萄糖苷。2)3,5-二咖啡酰奎宁酸甲酯。3)4,5-二咖啡酰奎宁酸甲酯。4)5-咖啡酰奎宁酸甲酯。5)马钱子苷。6)demethylsecologanol。[结论]其中化合物1、4为首次从川续断根中分离得到。     

3,5-O-二咖啡酰奎宁酸的合成

目的研究3,5-O-二咖啡酰奎宁酸的合成工艺。方法根据目标化合物3,5-O-二咖啡酰奎宁酸的结构特点,本文选择咖啡酸与奎宁酸作为合成原料,拟采用选择性地保护二者活泼官能团-酰氯成酯-脱保护的方法以得到目标产物。结果目标产物经NMR和MS分析为结构正确的化合物。结论本合成工艺提高了3,5-O-二咖啡酰奎宁酸的产率,但副产物较多,分离较困难。     

γ,δ-不饱和β-酮酯和羟基酸衍生的酰氯酰化反应

叙述了由γ,δ-不饱和β-酮酯与羟基酸衍生的酰氯,经酰化生成了6-酰氧基取代的5-乙酯基-2,3-二氢-4H-4-吡喃酮的衍生物,其中有些化合物可以转变为7-烷基或羟基取代的2,3-二氢-4,5-二氧(7H)呋喃并[3,4-b]吡喃衍生物。     

基于一测多评法测定银黄制剂中6种咖啡酰奎宁酸类成分的含量

[目的]建立测定银黄制剂中6种咖啡酰奎宁酸类成分(绿原酸、新绿原酸、隐绿原酸、异绿原酸A、异绿原酸B和异绿原酸C)含量的一测多评(QAMS)方法.[方法]采用Diamonsil C₁₈(4.6 mm×250.0 mm,5.0μm)色谱柱，以0.4%(体积分数)磷酸水溶液(A)-乙腈(B)为流动相进行梯度洗脱(流动速度为1.0 m L·min^-1，进样量10μL，检测波长327 nm，柱温30℃).以绿原酸(5-O-咖啡酰奎宁酸)为内参物，计算其他5种咖啡酰奎宁酸类成分的相对校正因子并计算该5种成分的含量，实现QAMS.同时采用外标法测定6种咖啡酰奎宁酸类成分的含量，并对两种方法测定的结果进行比较，验证QAMS法的可行性和准确性.[结果]在一定线性范围内，以绿原酸为内参，新绿原酸(3-O-咖啡酰奎宁酸)、隐绿原酸(4-O-咖啡酰奎宁酸)、异绿原酸C(4,5-O-二咖啡酰奎宁酸)、异绿原酸A (3,5-O-二咖啡酰奎宁酸)和异绿原酸B (3,4-O-二咖啡酰奎宁酸)5种成分的平均校正因子分别为1.224 3、0.956 9、0.814 1、0.8...     

苍耳子中咖啡酰奎宁酸类化学成分的研究

目的研究苍耳子中的化学成分。方法采用硅胶柱色谱、ODS反相柱色谱、Sephadex LH-20、MCI gel CHP 20P和HPLC等色谱方法进行分离纯化,并结合质谱与现代波谱学技术鉴定化合物结构。结果从苍耳子70%乙醇提取物中乙酸乙酯萃取部位分离得到10个化合物,分别鉴定为咖啡酸(1)、咖啡酸甲酯(2)、咖啡酸乙酯(3)、5-O-咖啡酰基奎宁酸(4)、5-O-咖啡酰基奎宁酸甲酯(5)、3-O-咖啡酰基奎宁酸甲酯(6)、1,5-O-二咖啡酰基奎宁酸(7)、3,5-O-二咖啡酰基奎宁酸(8)、3,5-O-二咖啡酰基奎宁酸甲酯(9),3,4-O-二咖啡酰基奎宁酸甲酯(10)。结论本实验所测定数据与文献报道的基本一致。     

双黄连吸入溶液UPLC指纹图谱及含量测定研究

目的 建立双黄连吸入溶液UPLC指纹图谱,并同时测定新绿原酸、绿原酸、隐绿原酸、异绿原酸B、3,5-二-O-咖啡酰奎宁酸、4,5-二-O-咖啡酰奎宁酸、连翘苷7种活性成分的含量.方法 采用Agilent 1290超高效液相色谱仪,以黄芩苷为参照物在210 nm波长下建立双黄连吸入溶液指纹图谱;在324 nm波长下对新绿...     

络石藤中的三萜类化合物

目的对络石藤中的三萜类化合物进行分离鉴定。方法采用硅胶、反相硅胶、Sephadex LH-20等柱色谱方法进行分离,NM R等波谱学方法进行结构鉴定。结果从络石藤中分离鉴定8个三萜类化合物:络石苷F(tra-che losperos ide F,Ⅰ)、络石苷B-1(trache losperos ide B-1,Ⅱ)、络石苷D-1(trache losperos ide D-1,Ⅲ)、络石苷E-1(trache losperos ide E-1,Ⅳ)、3-βO-D-g lucopyranos ide qu inov ic ac id(Ⅴ)、3β-O--βD-g lucopyrano isde qu inov ic ac id27-O--βD-g lucopyranosy l ester(Ⅵ)、3β-O--βD-g lucopyranos ide c incho lic ac id 27-O--βD-g lucopyranosy l ester(Ⅶ)、络石苷元B(trache losperogen in B,Ⅷ)。结论Ⅰ为新化合物,其余均为首次从该植物中分离得到。     

猫儿刺叶的化学成分研究

目的研究猫儿刺Ilex pernyi叶的化学成分。方法应用硅胶、Sephadex LH-20凝胶柱色谱法及半制备RP-HPLC对其化学成分进行分离,利用光谱数据并结合理化性质鉴定分离得到的化合物。结果从猫儿刺的干燥叶中分离鉴定了9个化合物,分别为β-谷甾醇(Ⅰ)、(6RS)-(Z)-2,6-dimethyl-2,7-octadiene-1,6-diol(Ⅱ)、(6RS)-(E)-2,6-dimethyl-2,7-octadiene-1,6-diol(Ⅲ)、8-hydroxypinoresinol(Ⅳ)、( )-fraxiresinol(Ⅴ)、blumenol A(Ⅵ)、胡萝卜苷(Ⅶ)、3,5-二咖啡酰奎宁酸丁酯(Ⅷ)、4、5-二咖啡酰奎宁酸丁酯(Ⅸ)。结论该9个化合物均为首次从猫儿刺中分离得到。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.