人角膜内皮细胞压力调节培养的初步研究

Objective To investigate the effect of pressure bionic culture on the morphology and function of rabbit corneal endothelial cells. Methods Corneal endothelial cells were separated and purified by tearing apart the descemet and digesting with trypsin and EDTA, then cultured in the plate. The cells were divided into two groups: group A were cultured under atmosphere; cells exposed to 2 kPa( 14. 66 mm Hg) pressure in vitro was group B; the morphology and growth pattern of cells were observed by inverted microscope; cells origin were identified by neuron-specific enolase immunoassay. Cellular changes in the structure were observed by HE staining and scanning and transmission electron microscopy (SEM and TEM) analysis. Cells activity was detected by flow cytometry. Results NSE antibody of the primary corneal endothelial cells was positive without corneal epithelial cells and corneal stroma cells. Two groups of cells were cultured for 120-144 h respectively, the morphology was flat, polygon, most of cells were hexagon and abundant cytoplasms in group B (pressure bionic culture), but in group A, the cells size was not uniform and there were much granules in the cytoplasm. There was no difference in the time of formation of monolayer in two groups. SEM showed that cells exposed to pressure connected tightly and the surface was rich in microvilli, extended foot processes and attached to the substrate tightly, while cells cultured under atmosphere with more off-chip. In group B, Annexiv-FITC/PI detection of apoptosis showed cell survival rate was 98.2%, early apoptosis rate was 0.7%, late apoptosis rate was 1.0%, death rate was 0. 1%; the corresponding data were 92.2%, 5.2%, 2.3%, and 0.3% in group A, respectively; There was statistically significant difference between the two groups (x2 =594. 0,P ＜0. 01 ). After cultured for 96 h,the expression of ZO-1 protein in cells exposed to pressure was higher than those in control. Conclusions The biological activity of endothelial cells is regulated positively by bionic pressure. The establishment of a new biomimetic pressure model will help to investigate the physiological function and injury repair of corneal endothelial cells in vitro.     

人角膜内皮细胞压力调节培养的初步研究

目的 探讨模拟前房压力对角膜内皮细胞形态及生物学功能的影响.方法 实验研究.采用后弹力层撕除联合酶消化法分离及纯化角膜内皮细胞,细胞悬液接种于培养板内,分为两组:A组采用常规的无压力培养;B组为压力仿生培养,压力设为2.0 kPa(14.66 mm Hg).倒置显微镜定期观察细胞形态及生长规律,神经元烯醇化酶免疫法鉴定细胞来源;苏木素-伊红染色及扫描和透射电镜分析细胞结构的变化,流式细胞术检测细胞活性.免疫荧光检测细胞间紧密连接蛋白(ZO-1)表达情况.结果 获取的细胞神经元烯醇化酶抗体表达阳性,证实为角膜内皮细胞表型,无角膜上皮细胞及基质细胞污染.两组细胞分别培养120～144 h后,压力仿生培养组见细胞扁平,胞质丰富,细胞排列紧密,呈铺路石状,六边形细胞居多;常规培养组细胞在形成单层的时间上与压力仿生培养组并无差异,但细胞以多角形为主,大小不一,细胞中颗粒样物质较多.扫描电镜下压力仿生培养组角膜内皮细胞形成连接紧密的单层,形态为多边形,表面微绒毛丰富,细胞间伸出足突相连,与底物贴附紧密,而常规培养组细胞脱片现象较明显.压力仿生培养组荧光标记的磷酯结合蛋白-碘化丙啶(Annexin V-FTTC/PI)细胞凋亡检测示细胞存活率为98.2%,早期凋亡率为0.7%,末期凋亡率为1.0%,死亡率为0.1%,而常规培养组细胞相对应上述检测值分别为92.2%,5.2%,2.3%及0.3%,经卡方检验两组比较差异存在统计学意义(x2=594.0,P＜0.01).培养96 h后压力培养组角膜内皮细胞间ZO-1蛋白表达明显高于对照组.结论 低压力对角膜内皮细胞生物学活性有正向调节作用,并且表现为时间敏感性,建立了全新的角膜内皮细胞压力仿生培养模式,为体外进行角膜内皮细胞的生理功能与损伤修复研究提供了新的平台.

Abstract：

Objective To investigate the effect of pressure bionic culture on the morphology and function of rabbit corneal endothelial cells. Methods Corneal endothelial cells were separated and purified by tearing apart the descemet and digesting with trypsin and EDTA, then cultured in the plate. The cells were divided into two groups: group A were cultured under atmosphere; cells exposed to 2 kPa( 14. 66 mm Hg) pressure in vitro was group B; the morphology and growth pattern of cells were observed by inverted microscope; cells origin were identified by neuron-specific enolase immunoassay. Cellular changes in the structure were observed by HE staining and scanning and transmission electron microscopy (SEM and TEM) analysis. Cells activity was detected by flow cytometry. Results NSE antibody of the primary corneal endothelial cells was positive without corneal epithelial cells and corneal stroma cells. Two groups of cells were cultured for 120-144 h respectively, the morphology was flat, polygon, most of cells were hexagon and abundant cytoplasms in group B (pressure bionic culture), but in group A, the cells size was not uniform and there were much granules in the cytoplasm. There was no difference in the time of formation of monolayer in two groups. SEM showed that cells exposed to pressure connected tightly and the surface was rich in microvilli, extended foot processes and attached to the substrate tightly, while cells cultured under atmosphere with more off-chip. In group B, Annexiv-FITC/PI detection of apoptosis showed cell survival rate was 98.2%, early apoptosis rate was 0.7%, late apoptosis rate was 1.0%, death rate was 0. 1%; the corresponding data were 92.2%, 5.2%, 2.3%, and 0.3% in group A, respectively; There was statistically significant difference between the two groups (x2 =594. 0,P ＜0. 01 ). After cultured for 96 h,the expression of ZO-1 protein in cells exposed to pressure was higher than those in control. Conclusions The biological activity of endothelial cells is regulated positively by bionic pressure. The establishment of a new biomimetic pressure model will help to investigate the physiological function and injury repair of corneal endothelial cells in vitro.     

Density and morphology of corneal endothelial cell after phacoemulsification using ringer lactate versus balanced salt solution as irrigating solutions

AIM: To compare the difference in corneal endothelial cell density and morphology after phacoemulsification using ringer lactate (RL) and balanced salt solution (BSS) irrigating solutions. METHODS: The prospective randomized controlled trial study was conducted between February 2017 and April 2017 in Dr. YAP Eye Hospital, Yogyakarta, Indonesia. There were a total of 52 subjects (52 eyes) who were senile cataract patients further grouped into two, 26 patients undergoing the phacoemulsification procedure using RL irrigating solution and the other 26 patients with BSS irrigating solution, both conducted by one operator. On the 1, 7, and 28d post operative, an evaluation was done to measure the density and corneal endothelial cell morphology, as well as the variable of inflammation in the two groups. RESULTS: Fifty-two eyes had undergone phacoemulsification with posterior intraocular lens implantation. Both groups were evaluated for the endothelial cell reduction and corneal endothelial cell morphology change, along with postoperative inflammation. On the 28d postoperative, endothelial cell reduction in the BSS group (173.96 cell/mm2, 8.12%) was lower than the RL group (253.20 cell/mm2, 10.25%), percentage of corneal endothelial cell variation coefficient increase in the BSS group (2.92%, 8.36%) was lower compared to the RL group (3.42%, 9.96%), decrease of hexagonal cells of corneal endothelium cells presentation percentage in the BSS group (4.30%, 8.17%) was lower compared to the RL group (4.84%, 8.97%), and the percentage increase of central corneal thickness in the BSS group (4.69 μm, 0.89%) was almost equal to the RL group (4.53 μm, 0.90%). All of the results regarding difference in density and corneal cell endothelium morphology between the two groups did not reveal any statistically significant difference (P>0.05). Inflammatory variable in the two groups were even. CONCLUSION: BSS and RL were equal in their capability of maintaining endothelial cell loss and endothelial cell morphologic change in senile cataract patients after phacoemulsification.     

Efficacy of poly (lactic-co-glycolic acid) and tetrandrine-loaded nanomicrospheres in the treatment of dry eye北大核心

Objective To discover a nano-drug with anti-inflammatory, sustained-release, and biocompatible properties aimed at blocking the dry eye formation pathway. Methods Nano-microspheres (Tet-ATS@PLGA) composed of tetrandrine (Tet) and poly (lactic-co-glycolic acid) (PLGA) were prepared using the thin-film hydration method, and their stability at room temperature (25 ℃), encapsulation efficiency, and drug loading were tested. Normal rabbit eyes without intervention were recruited in the normal group, and dry eye models were randomly divided into the control group (without any intervention), ATS group (intervened by artificial tears), Tet-ATS group (intervened by artificial tears and Tet), and Tet-ATS@PLGA group (intervened by Tet-ATS@PLGA). Flow cytometry was performed to detect the apoptosis of inflammatory corneal epithelial cells in each group after 24 hours of intervention. The staining of corneal epithelial cells, tear film break-up time (BUT), and surface tear secretion (detected by the Schirmer test strip) were recorded after 14 days of intervention. The thickness of corneal epithelial cells as well as the shape and number of bulbar conjunctival goblet cells were monitored by hematoxylineosin staining. Corneal proteins were extracted for the enzyme-linked immunosorbent assay to measure the expression levels of vascular endothelial growth factor (VEGF), interleukin-1β (IL-1β), prostaglandin E2 (PGE2), and tumor necrosis factor-α (TNF-α). The independent samples t-test was carried out for comparison among groups. Results The encapsulation efficiency and drug loading of Tet-ATS@PLGA nano-drug were 77.43% and 30.26%, respectively. The drug was stable at room temperature and easy to release when the ocular surface temperature stood at 33 ℃. Compared with other groups, BUT and the amount of tear secretion in the Tet-ATS@PLGA group were the largest, the thickness of corneal epithelial cells was close to the normal value, bulbar conjunctival goblet cells recovered the most, the apoptosis of inflammatory corneal epithelial cells after 24 hours of intervention was the most significant, and the expression levels of VEGF, IL-1β, TNF-α, and PGE2 were the lowest (all P<0.05). Conclusion Tet-ATS@PLGA nano-drug can effectively act on inflammatory corneal epithelial cells in rabbits, promote apoptosis of inflammatory cells, and block the inflammatory response of dry eyes by inhibiting the expression of VEGF, IL-1β, TNF-α, and PGE2, thus improving tear secretion on the ocular surface. © The Author(s) 2023.     

Human β-NGF gene transferred to cat corneal endothelial cells

AIM: To transfect the cat corneal endothelial cells (CECs) with recombinant human β-nerve growth factor gene adeno-associated virus (AAV-β-NGF) and to observe the effect of the expressed β-NGF protein on the proliferation activity of cat CECs. METHODS: The endothelium of cat cornea was torn under the microscope and rapidly cultivated in Dulbecco’s modified Eagle''s medium (DMEM) to form single layer CECs and the passage 2 endothelial cells were used in this experiment. The recombinant human AAV-β-NGF was constructed. The recombinant human AAV-β-NGF was transferred into cat CECs directly. Three groups were as following: normal CEC control group, CEC-AAV control group and recombinant CEC-AAV-β-NGF group. Forty-eight hours after transfection, the total RNA was extracted from the CEC by Trizol. The expression of the β-NGF target gene detected by fluorescence quantitative polymerase chain reaction; proliferation activity of the transfected CEC detected at 48h by MTT assay; the percentage of G1 cells among CECs after transfect was detected by flow cytometry method (FCM); cell morphology was observed under inverted phase contrast microscope. RESULTS: The torn endothelium culture technique rapidly cultivated single layer cat corneal endothelial cells. The self-designed primers for the target gene and reference gene were efficient and special confirmed through electrophoresis analysis and DNA sequencing. Forty-eight hours after transfect, the human β-NGF gene mRNA detected by fluorescence quantitative polymerase chain reaction showed that there was no significant difference between normal CEC control group and CEC-AAV control group (P>0.05); there was significant difference between two control groups and recombinant CEC-AAV-β-NGF group (P<0.05). MTT assay showed that transfect of recombinant AAV-β-NGF promoted the proliferation activity of cat CEC, while there was no significant difference between normal CEC control group and CEC-AAV control group (P>0.05). FCM result showed that the percentage of G1cells in CEC-AAV-NGF group was 76.8% while that in normal CEC control group and CEC-AAV control group was 46.6% and 49.8%. CONCLUSION: Recombinant AAV-β-NGF promotes proliferation in cat CECs by expressing bioactive β-NGF protein in high efficiency and suggests that its modulation can be used to treat vision loss secondary to corneal endothelial dysfunction.     

EXPERIMENTAL STUDY ON THE CORNEAL ENDOTHELIUM OF TRAUMATIC CATARACT

The cell morphology of corneal endothelium in 84 mice with experimental traumatic cataract was investigated with stained corneal buttons. In the experimental group, the boundaries between adjacent corneal endothelial cells were significantly distorted, some cell boundaries manifested degenerative changes that led to coalescence of the cells. The mean density and mean area of endothelial cells of the controls showed significant difference from those of the experimental group during the 12 weeks of observation. The density of endothelial cells decreased from 3312±337/mm~2 of the control group to 2944±418/mm~2 in the group of partially opaque lenses and 2713±472/mm~2 in the group of totally opaque lenses. Meanwhile, the area of endothelial cells and the coefficient of variation also significantly changed correspondingly, and the degree of damage in the corneal endothelium correlated with the degree of the lens opacity.     

Preliminary studies of constructing a tissue-engineered lamellar corneal graft by culturing mesenchymal stem cells onto decellularized corneal matrix

AIM:To construct a competent corneal lamellar substitute in order to alleviate the shortage of human corneal donor.METHODS:Rabbit mesenchymal stem cells(MSCs)were isolated from bone marrow and identified by flow cytometric,osteogenic and adipogenic induction.Xenogenic decellularized corneal matrix(XDCM)was generated from dog corneas.MSCs were seeded and cultured on XDCM to construct the tissueengineered cornea.Post-transplantation biocompatibility of engineered corneal graft were tested by animal experiment.Rabbits were divided into two groups then underwent lamellar keratoplasty(LK)with different corneal grafts:1)XDCM group(n=5):XDCM;2)XDCM-MSCs groups(n=4):tissue-engineered cornea made up with XDCM and MSCs.The ocular surface recovery procedure was observed while corneal transparency,neovascularization and epithelium defection were measured and compared.In vivo on focal exam was performed 3 mo postoperatively.RESULTS:Rabbit MSCs were isolated and identified.Flow cytometry demonstrated isolated cells were CD90 positive and CD34,CD45 negative.Osteogenic and adipogenic induction verified their multipotent abilities.MSC-XDCM grafts were constructed and observed.In vivo transplantation showed the neovascularization in XDCMMSC group was much less than that in XDCM group postoperatively.Post-transplant 3-month confocal test showed less nerve regeneration and bigger cell-absent area in XDCM-MSC group.CONCLUSION:This study present a novel corneal tissue-engineered graft that could reduce post-operatively neovascularization and remain transparency,meanwhile shows that co-transplantation of MSCs may help increase corneal transplantation successful rate and enlarge the source range of corneal substitute to overcome cornea donor shortage.     

全反式视黄酸对诱导为神经元样细胞的脐带间充质干细胞分化和凋亡的影响

Objective To evaluate the role of different concentration of all-trans retinoic acid (ATRA)on the morphology,proliferation and apoptosis in inducing umbilical cord mesenchymal stem cells (MSC) into neuron-like cells in vitro,and screen the optimal concentration of ATRA.Methods It was an experimental study.The third passage of MSC was placed in 24-well cell culture plates at a density of 1×104/well.After the adherent of cells,the medium was changed to DMEM/F-12 containing different concentration of ATRA(0.25 μmol/L,0.5 μmol/L,1.0 μmol/L,2.0 μmol/L,4.0 μmol/L)for 24 h respectively.The cells cultured without ATRA were taken as the coutrol group.After another 24 h,the morphologic changes of induced cells were observed by inverted microscope and cell proliferation,apoptosis of ATRA was analyzed using the MTT colorimetric assay.We take another control group and ATRA groups to detect the apoptotic and positive stained percentage of induced cells by Annexin V-FITC/PI combining flow cytometry.The optimal concentration of ATRA was determined by all the above-mentioned index.According to the nature of the material,analysis of variance (ANOVA) was employed for absorption value and apoptosis rate in different concentration of ATRA for 24 h,t test for further comparision between two groups.T-test were also used between the positive expression of induced neuron-like cells and the control group.Results Compared to the control group,ATRA at the concentration of 0.25 μmoL/L did not inhibit the proliferation of umbilical cord MSC obviously(t=0.72.1.32,P>0.05).Part of MSC wele floating instantly at the moment of adding ATRA of 4.0 μmoL/L and no adherent cells were observed after 24 h'culture.Exposed to ATRA at the concentration of≥1.0 μmol/L for 24 h,the proliferation of MSC were significantly inhibited,showing a dose-dependent manner(t=8.8,18.9,22.1;P<0.01).0.5 μmol/L of ATRA did not affect the proliferation of cells and its moephology remained normal;1.0 μmol/L of ATRA affected very few cells;but 2.0 μmoL/L of ATRA cultured for 24 h inhibited the proliferation of cells obviously than 1 h.and the cells increased in size and became flattened.Flow cytometry showed that the rate of apoptosis between the control group and≥1.0 μmol/L groups were significantly different(t=9.88,19.95,31.61;P<0.01).Conclusion In the process of inducing umbilical cord MSC into neuron-like cells,0.5 μmol/L ATRA was the optical concentration.≥1.0 μmol/L ATRA can inhibit the cell proliferation,increase the apoptosis of cells significantly and caused obvious damages.     

全反式视黄酸对诱导为神经元样细胞的脐带间充质干细胞分化和凋亡的影响

Objective To evaluate the role of different concentration of all-trans retinoic acid (ATRA)on the morphology,proliferation and apoptosis in inducing umbilical cord mesenchymal stem cells (MSC) into neuron-like cells in vitro,and screen the optimal concentration of ATRA.Methods It was an experimental study.The third passage of MSC was placed in 24-well cell culture plates at a density of 1×104/well.After the adherent of cells,the medium was changed to DMEM/F-12 containing different concentration of ATRA(0.25 μmol/L,0.5 μmol/L,1.0 μmol/L,2.0 μmol/L,4.0 μmol/L)for 24 h respectively.The cells cultured without ATRA were taken as the coutrol group.After another 24 h,the morphologic changes of induced cells were observed by inverted microscope and cell proliferation,apoptosis of ATRA was analyzed using the MTT colorimetric assay.We take another control group and ATRA groups to detect the apoptotic and positive stained percentage of induced cells by Annexin V-FITC/PI combining flow cytometry.The optimal concentration of ATRA was determined by all the above-mentioned index.According to the nature of the material,analysis of variance (ANOVA) was employed for absorption value and apoptosis rate in different concentration of ATRA for 24 h,t test for further comparision between two groups.T-test were also used between the positive expression of induced neuron-like cells and the control group.Results Compared to the control group,ATRA at the concentration of 0.25 μmoL/L did not inhibit the proliferation of umbilical cord MSC obviously(t=0.72.1.32,P>0.05).Part of MSC wele floating instantly at the moment of adding ATRA of 4.0 μmoL/L and no adherent cells were observed after 24 h'culture.Exposed to ATRA at the concentration of≥1.0 μmol/L for 24 h,the proliferation of MSC were significantly inhibited,showing a dose-dependent manner(t=8.8,18.9,22.1;P<0.01).0.5 μmol/L of ATRA did not affect the proliferation of cells and its moephology remained normal;1.0 μmol/L of ATRA affected very few cells;but 2.0 μmoL/L of ATRA cultured for 24 h inhibited the proliferation of cells obviously than 1 h.and the cells increased in size and became flattened.Flow cytometry showed that the rate of apoptosis between the control group and≥1.0 μmol/L groups were significantly different(t=9.88,19.95,31.61;P<0.01).Conclusion In the process of inducing umbilical cord MSC into neuron-like cells,0.5 μmol/L ATRA was the optical concentration.≥1.0 μmol/L ATRA can inhibit the cell proliferation,increase the apoptosis of cells significantly and caused obvious damages.     

Y27632体外诱导视网膜色素上皮细胞转分化为神经元样细胞的初步研究

Objective To investigate the feasibility of Y27632 to induce transdifferentiation from human retinal pigment epithelial(hRPE)cells into neuron-like cells in vitro.Methods The third to sixth generation of primary hRPE cells were cultured with 2% fetal bovine serum+Dulbecco's modified eagle medium/F12 culture solution,with(experimental group)or without(control group)10 μmol/L Y27632.At 3,6 hours and 1,3,5,7 days after induction,the morphologic changes of RPE cells were observed by inverted microscope.The expression rate of CK18,Map2,NF200 and Pax6 at 3 days after induction in the experimental and control group were detected by immunofluorescent staining.χ2 test was employed for comparison between the two groups.Results 50.1% cells of the experimental group formed axon-like processes and interconnected each other with typical neuron-like appearance.The expression rates of CK18,Map2,NF200 and Pax6 in the experimental group were 43.88% ,31.90% ,57.45% and 65.79% .while the above indexes in the control group were 93.97% ,4.49% ,22.37% and 8.33% respectively.Compared the expression rate of CK18(χ2=64.763),Map2(χ2=23.634),NF200(χ2=21.261)and Pax6(χ2=25.946)between the two groups,the differences were significant(P＜0.01).Conclusion The hRPE cells can be trans-differentiated into neuron-like cells in vitro bv Y27632.     

角膜曲率的分析

目的：探讨我国人角膜曲率半径的正常值及不同性别、不同年龄的角膜曲率半径差异。方法：对10998只眼的角膜曲率进行检测，并按男、女10岁一组进行统计分析。结果：（1）K1为7.65mm,K2为7.71mm，平均K值为7.67mm。较眼科学正常值K:7.77mm短0.1mm。（2）K的平均值男性较女性的长0.1155mm。且女性各年龄段角膜曲率半径均男性的有不同程度的减短。（3）男女均随年龄的增长，角膜曲率半径大致呈递减趋势，即：角膜曲率半径与年龄成反比关系。（４）男女K1,K2之比，均随年龄增长而增长，即K1逐渐增长而增长，即　K1值逐渐增长，K2逐渐减短。结论：本文测定的角膜曲率较眼科文献中的提供的正常值短0.1mm，并且存在着年龄、性别上的差异。     

艾滋病眼部并发症的资料分析

目的:通过调查分析,了解艾滋病眼部并发症的临床表现、治疗及预后。方法:收集赞比亚卡布韦总医院眼科2008-08/2009-08就诊患者。结果:艾滋病眼部并发症症状重,病程长,致盲率高。结论:充分认识、掌握艾滋病眼部并发症的临床表现,提高艾滋病的检出率,早发现、早治疗,提高艾滋病患者的生活质量。同时应强调预防是降低艾滋病发生率的关键。     

亟待新型早产儿视网膜病变动物模型的问世

早产儿视网膜病变（ROP）病因和发病机制尚不完全清楚,制约了其有效防治和相关研究的深入开展。尽管氧诱导视网膜病变动物模型为探索ROP复杂的病因和发病机制发挥了重要作用,但特异性较差,与人类ROP临床本质存在一定差异。因此,有必要对现有动物模型进行改良或建立新动物模型。通过更新观念、在多学科交叉中寻求突破,融合更多ROP危险因素,并结合新兴的转基因技术以及完善模型评价系统,建立科学的实验研究平台,为更好地开展ROP防治研究奠定基础。     

泪囊鼻腔吻合术后复发的原因分析和再次手术探讨

目的分析泪囊鼻腔吻合术术后复发的原因并探讨再次手术的可行性。方法对11例泪囊鼻腔吻合术术后复发的患者予以再手术治疗,随访半年至一年。结果复发原因：泪囊未完全切开2例,骨孔过小或位置错误4例,吻合腔或骨孔内肉芽组织增生堵塞5例;10例术后流泪消失,泪道冲洗通畅,占90.91%,1例流泪减轻,泪道冲洗基本畅通,占9.09%。结论泪囊鼻腔吻合术术后复发患者可以通过再次手术重建泪道,是治疗泪囊鼻腔吻合术术后复发的有效方法之一。     

早产儿视网膜病变阈值病变的相关因素分析

目的 对早产儿视网膜病变(ROP)不同转归一自然退行与阈值病变各相关因素进行分析,以探讨与ROP阈值病变有关的因素.方法 回顾性分析2008年5月至2009年7月在吉林大学第一医院新生儿科收治的83例确诊ROP的早产儿相关临床数据,并进行统计学分析.结果 在83例不同程度ROP患儿中(166只眼),自然退行51例(102只眼,占ROP患儿61.45％),阈值病变32例(64只眼,占ROP患儿38.55％).使用t检验和logistic回归分析,结果表明ROP阈值病变组的胎龄较ROP自然退行组小,产次较ROP自然退行组次数多,差异均有统计学意义(P＜0.05);排除其他因素干扰后,男性、机械呼吸及发生败血症与ROP阈值病变有关,组间差异均有统计学意义(P＜0.05).结论 小胎龄、多产次,男性、使用机械呼吸、发生败血症与ROP不能自然退行有关.     

多排螺旋CT三维重建对视神经管骨折的诊断价值

目的探讨多排螺旋CT对视神经管骨折的诊断价值。方法对58例眼部外伤的病人行螺旋CT扫描，并进行三维重建。结果58例病人中，均伴不同程度的眼眶骨折，合并视经管骨折8例，其中视神经管内壁骨折4例，内壁为蝶窦壁3例，筛窦壁1例，外壁骨折3例，上壁骨折1例，通过三维重建可清晰地显示骨折线，骨重建显示准确，多平面重建定位好，3D重建显示骨折直观、立体。结论多排螺旋CT三维重建对视神经管骨折显示清晰，定位准确，对临床论断及治疗提供充分的依据。     

早产儿视网膜病变筛查和阈值期治疗的研究

目的研究早产儿视网膜病变(ROP)的发生率,评估ROP阈值期治疗效果。方法使用双目间接检眼镜对108例早产儿进行ROP筛查,将筛查结果进行统计学分析,达到阈值病变的患儿及时进行视网膜激光光凝或经巩膜、视网膜冷凝术。结果筛查108例早产儿,发现ROP23例,发生率为21.3%。在所有ROP患儿中,ROP1期13例,占56.5%;ROP2期3例,占13.0%;ROP3期7例,占30.4%。其中ROP3期患儿均伴有附加病变,达到阈值病变标准。ROP患儿出生体重为(1.43±0.25)kg(t=4.059,P<0.001);孕周为(31.0±2.3)周(t=2.637,P=0.013);吸氧时间为1~49d,平均17d(n=23,Z=-3.630,P<0.001);需要机械辅助呼吸患儿18例(χ2=12.009,P=0.001);上述指标与非ROP患儿比较,差异均有统计学意义;而与是否多胎的差异无统计学意义(χ2=1.013,P=0.314)。Logistic回归分析:出生体重低(β=-2.542,OR=0.079,P=0.032)和使用机械辅助呼吸(β=1.341,OR=3.823,P=0.025)的患儿是发生ROP的相关高危因素。7例阈值期病变患儿中,6例进行激光光凝或冷凝治疗。术后随访2个月至2年,手术眼的结构和视功能未见异常。1例阈值期病变患儿未予治疗,于1个月后出现视网膜脱离。结论出生体重轻、孕周少、吸氧时间长、需要机械辅助呼吸的早产儿发生ROP的风险较高。对阈值期病变患儿应及时进行激光光凝或冷凝治疗。     

玻璃体内注射康柏西普治疗早产儿视网膜病变的血管化过程

目的：评价玻璃体内注射康柏西普在治疗I型(阈值期和阈值前期)和A-ROP(急进性ROP)的早产儿视网膜病变(ROP)的一系列病例中引起的视网膜血管化过程。方法：回顾性研究2017-07/2020-03在厦门市儿童医院眼科通过玻璃体腔注射康柏西普(IVC)治疗的ROP患者34例67眼。再活化是指急性期特征的复发，发生在疾病的任何阶段，无论是否存在其他疾病。结果：患儿34例的平均胎龄为28.82±2.32wk。平均出生体质量为1155.18±398.22g。19例37眼的病变区域为Ⅰ区。10例20眼的病变位于Ⅱ区，5例10眼的病变位于Ⅱ区后部。一次IVC治疗的ROP患儿疾病控制总有效率为73.1%(49/67)，且Ⅱ区血管化均完成。患者在Ⅲ区的血管化完成率出现差异。在接受过一次治疗且未再复发的患者中，Ⅰ型ROP血管化时间平均为9.11±2.49wk，A-ROP为13.40±4.04wk。A-ROP的血管化完成时间明显比Ⅰ型ROP的时间长，且结果有统计学差异。结论：IVC治疗后的病变为Ⅱ区的患儿均具有较高的血管完成率。     

中国早产儿视网膜病变登记网的建设

早产儿视网膜病变（ROP）近年来在我国逐渐受到关注,成为研究热点.但由于民众欠缺相关知识及专业技术人员的匮乏,致使我国ROP的防治处于初级阶段,亟待ROP知识的普及以及专业防治的开展.＂中国早产儿视网膜病变登记网＂（http：//www.chinarop.com）的建设,旨在加强ROP的科普宣传和医务工作者的专业培训,建立国内的ROP病历注册登记制度及ROP数据库,为国内的ROP研究提供帮助.     

眼睑分裂痣的手术方式探讨

目的 探讨不同部位和大小眼睑分裂痣的手术方式及效果.方法 回顾性系列病例研究.收集本中心1997年7月至2006年11月的眼睑分裂痣病例共30例(30只眼),按不同部位,侵犯范围的大小分别采取了不同的手术方法.包括:分裂痣切除后直接缝合3例;分裂痣切除后Ⅰ期行游离皮肤移植术5例;分裂痣切除后皮瓣转移术,手术分两期进行,Ⅰ期行上睑或下睑手术,3个月后Ⅱ期行另一眼睑手术,共22例,其中对侵及泪小点的分裂痣将色素痣和受侵犯的泪小点一起切除后行泪小管口再造和睑成形术8例.结果 30例患者随访2～11年,平均随访时间60个月.有1例术后2年复发,随诊5年再次手术,术后病理发现有恶变,经再次行Mohs眼睑恶性肿瘤组织学控制性切除术,术后未见复发.其余病例未见复发和恶变.结论 眼睑分裂痣切除术采用分期手术转移皮瓣法修复眼睑比游离植皮法疗效更满意.对分裂痣切除术后的病例需密切观察,以便早期发现恶变.