腹泻患儿弗劳地枸橼酸盐杆菌耐药性研究

目的　探讨弗劳地枸橼酸盐杆菌的耐药性。方法　应用纸片扩散法检测弗劳地枸橼酸盐杆菌对抗生素的耐药性。用双纸片协同试验检测ESBL。结果　绝大多数受检菌对抗生素耐药。检出ESBL菌 5株 ,对氨苄青、羧苄青及头孢菌素具有广泛耐药性。结论　掌握病原菌的耐药谱 ,加强抗生素的管理和使用对细菌性传染病的防治具有重要意义。     

一株产新型大环内酯抗生素海洋解淀粉芽孢杆菌的分离鉴定

目的 对分离自中国东海30 m深海泥的一株产新型大环内酯抗生素(macrolactin s,MLS)的菌株进行形态、生理生化特性及分子水平的分类学研究.方法 通过细菌培养特征观察,菌株生长条件测定、生理生化指标及全细胞脂肪酸成分分析,并特异性扩增其16S rDNA序列,测序比对及构建系统发育进化树,利用本实验室所建立的抗菌模型评价代谢产物的抗菌活性.结果 该菌株与芽孢杆菌属其他种共享97%～99%的保守序列,其中与解淀粉芽孢杆菌(Bacillus amyloliquefaciens)的16SrDNA序列保守度高达99.6%.15:1 iso H/13:0 3OH为该菌株的主要脂肪酸组分.结论 该菌株属于芽孢杆菌属,定名为解淀粉芽孢杆菌MS1721株,其所产大环内酯MLS表现出较强的抗菌活性.     

一株产新型大环内酯抗生素海洋解淀粉芽孢杆菌的分离鉴定

Objective To perform taxonomic studies on a novel macrolactin-producing gram-positive bacterial strain MS1721 isolated from the mud at a depth of 30 m in the East China Sea. Methods Taxonomic studies were conducted based on the phenotypic and BIOLOG analysis, 16S rDNA gene sequencing and blast analysis, and fatty acid components analysis. The antibacterial activity of macrolactin S was determined by evaluation models established in our lab. Results MS1721 strain displayed high similarity with genus Bacillus in similarity values between 97%～99%, in which the nearest neighbor was Bacillus amyloliquefaciens with the similarity value of 99.6%. The predominant fatty acid component was 15:1 iso H/13:0 3OH. Conclusions MS1721 strain belongs to the genus Bacillus, and Bacillus amyloliquefaciens MS1721 was proposed as the name for this strain on the basis of the taxonomic data. The second metabolite-macrolactin s produced by this strain exhibited obvious antibacterial activity.     

一株产新型大环内酯抗生素海洋解淀粉芽孢杆菌的分离鉴定

Objective To perform taxonomic studies on a novel macrolactin-producing gram-positive bacterial strain MS1721 isolated from the mud at a depth of 30 m in the East China Sea. Methods Taxonomic studies were conducted based on the phenotypic and BIOLOG analysis, 16S rDNA gene sequencing and blast analysis, and fatty acid components analysis. The antibacterial activity of macrolactin S was determined by evaluation models established in our lab. Results MS1721 strain displayed high similarity with genus Bacillus in similarity values between 97%～99%, in which the nearest neighbor was Bacillus amyloliquefaciens with the similarity value of 99.6%. The predominant fatty acid component was 15:1 iso H/13:0 3OH. Conclusions MS1721 strain belongs to the genus Bacillus, and Bacillus amyloliquefaciens MS1721 was proposed as the name for this strain on the basis of the taxonomic data. The second metabolite-macrolactin s produced by this strain exhibited obvious antibacterial activity.     

一株产新型大环内酯抗生素海洋解淀粉芽孢杆菌的分离鉴定

Objective To perform taxonomic studies on a novel macrolactin-producing gram-positive bacterial strain MS1721 isolated from the mud at a depth of 30 m in the East China Sea. Methods Taxonomic studies were conducted based on the phenotypic and BIOLOG analysis, 16S rDNA gene sequencing and blast analysis, and fatty acid components analysis. The antibacterial activity of macrolactin S was determined by evaluation models established in our lab. Results MS1721 strain displayed high similarity with genus Bacillus in similarity values between 97%～99%, in which the nearest neighbor was Bacillus amyloliquefaciens with the similarity value of 99.6%. The predominant fatty acid component was 15:1 iso H/13:0 3OH. Conclusions MS1721 strain belongs to the genus Bacillus, and Bacillus amyloliquefaciens MS1721 was proposed as the name for this strain on the basis of the taxonomic data. The second metabolite-macrolactin s produced by this strain exhibited obvious antibacterial activity.     

一株产新型大环内酯抗生素海洋解淀粉芽孢杆菌的分离鉴定

Objective To perform taxonomic studies on a novel macrolactin-producing gram-positive bacterial strain MS1721 isolated from the mud at a depth of 30 m in the East China Sea. Methods Taxonomic studies were conducted based on the phenotypic and BIOLOG analysis, 16S rDNA gene sequencing and blast analysis, and fatty acid components analysis. The antibacterial activity of macrolactin S was determined by evaluation models established in our lab. Results MS1721 strain displayed high similarity with genus Bacillus in similarity values between 97%～99%, in which the nearest neighbor was Bacillus amyloliquefaciens with the similarity value of 99.6%. The predominant fatty acid component was 15:1 iso H/13:0 3OH. Conclusions MS1721 strain belongs to the genus Bacillus, and Bacillus amyloliquefaciens MS1721 was proposed as the name for this strain on the basis of the taxonomic data. The second metabolite-macrolactin s produced by this strain exhibited obvious antibacterial activity.     

一株产新型大环内酯抗生素海洋解淀粉芽孢杆菌的分离鉴定

Objective To perform taxonomic studies on a novel macrolactin-producing gram-positive bacterial strain MS1721 isolated from the mud at a depth of 30 m in the East China Sea. Methods Taxonomic studies were conducted based on the phenotypic and BIOLOG analysis, 16S rDNA gene sequencing and blast analysis, and fatty acid components analysis. The antibacterial activity of macrolactin S was determined by evaluation models established in our lab. Results MS1721 strain displayed high similarity with genus Bacillus in similarity values between 97%～99%, in which the nearest neighbor was Bacillus amyloliquefaciens with the similarity value of 99.6%. The predominant fatty acid component was 15:1 iso H/13:0 3OH. Conclusions MS1721 strain belongs to the genus Bacillus, and Bacillus amyloliquefaciens MS1721 was proposed as the name for this strain on the basis of the taxonomic data. The second metabolite-macrolactin s produced by this strain exhibited obvious antibacterial activity.     

一株产新型大环内酯抗生素海洋解淀粉芽孢杆菌的分离鉴定

Objective To perform taxonomic studies on a novel macrolactin-producing gram-positive bacterial strain MS1721 isolated from the mud at a depth of 30 m in the East China Sea. Methods Taxonomic studies were conducted based on the phenotypic and BIOLOG analysis, 16S rDNA gene sequencing and blast analysis, and fatty acid components analysis. The antibacterial activity of macrolactin S was determined by evaluation models established in our lab. Results MS1721 strain displayed high similarity with genus Bacillus in similarity values between 97%～99%, in which the nearest neighbor was Bacillus amyloliquefaciens with the similarity value of 99.6%. The predominant fatty acid component was 15:1 iso H/13:0 3OH. Conclusions MS1721 strain belongs to the genus Bacillus, and Bacillus amyloliquefaciens MS1721 was proposed as the name for this strain on the basis of the taxonomic data. The second metabolite-macrolactin s produced by this strain exhibited obvious antibacterial activity.     

一株产新型大环内酯抗生素海洋解淀粉芽孢杆菌的分离鉴定

Objective To perform taxonomic studies on a novel macrolactin-producing gram-positive bacterial strain MS1721 isolated from the mud at a depth of 30 m in the East China Sea. Methods Taxonomic studies were conducted based on the phenotypic and BIOLOG analysis, 16S rDNA gene sequencing and blast analysis, and fatty acid components analysis. The antibacterial activity of macrolactin S was determined by evaluation models established in our lab. Results MS1721 strain displayed high similarity with genus Bacillus in similarity values between 97%～99%, in which the nearest neighbor was Bacillus amyloliquefaciens with the similarity value of 99.6%. The predominant fatty acid component was 15:1 iso H/13:0 3OH. Conclusions MS1721 strain belongs to the genus Bacillus, and Bacillus amyloliquefaciens MS1721 was proposed as the name for this strain on the basis of the taxonomic data. The second metabolite-macrolactin s produced by this strain exhibited obvious antibacterial activity.     

一株产新型大环内酯抗生素海洋解淀粉芽孢杆菌的分离鉴定

Objective To perform taxonomic studies on a novel macrolactin-producing gram-positive bacterial strain MS1721 isolated from the mud at a depth of 30 m in the East China Sea. Methods Taxonomic studies were conducted based on the phenotypic and BIOLOG analysis, 16S rDNA gene sequencing and blast analysis, and fatty acid components analysis. The antibacterial activity of macrolactin S was determined by evaluation models established in our lab. Results MS1721 strain displayed high similarity with genus Bacillus in similarity values between 97%～99%, in which the nearest neighbor was Bacillus amyloliquefaciens with the similarity value of 99.6%. The predominant fatty acid component was 15:1 iso H/13:0 3OH. Conclusions MS1721 strain belongs to the genus Bacillus, and Bacillus amyloliquefaciens MS1721 was proposed as the name for this strain on the basis of the taxonomic data. The second metabolite-macrolactin s produced by this strain exhibited obvious antibacterial activity.     

一株产新型大环内酯抗生素海洋解淀粉芽孢杆菌的分离鉴定

Objective To perform taxonomic studies on a novel macrolactin-producing gram-positive bacterial strain MS1721 isolated from the mud at a depth of 30 m in the East China Sea. Methods Taxonomic studies were conducted based on the phenotypic and BIOLOG analysis, 16S rDNA gene sequencing and blast analysis, and fatty acid components analysis. The antibacterial activity of macrolactin S was determined by evaluation models established in our lab. Results MS1721 strain displayed high similarity with genus Bacillus in similarity values between 97%～99%, in which the nearest neighbor was Bacillus amyloliquefaciens with the similarity value of 99.6%. The predominant fatty acid component was 15:1 iso H/13:0 3OH. Conclusions MS1721 strain belongs to the genus Bacillus, and Bacillus amyloliquefaciens MS1721 was proposed as the name for this strain on the basis of the taxonomic data. The second metabolite-macrolactin s produced by this strain exhibited obvious antibacterial activity.     

一株产新型大环内酯抗生素海洋解淀粉芽孢杆菌的分离鉴定

Objective To perform taxonomic studies on a novel macrolactin-producing gram-positive bacterial strain MS1721 isolated from the mud at a depth of 30 m in the East China Sea. Methods Taxonomic studies were conducted based on the phenotypic and BIOLOG analysis, 16S rDNA gene sequencing and blast analysis, and fatty acid components analysis. The antibacterial activity of macrolactin S was determined by evaluation models established in our lab. Results MS1721 strain displayed high similarity with genus Bacillus in similarity values between 97%～99%, in which the nearest neighbor was Bacillus amyloliquefaciens with the similarity value of 99.6%. The predominant fatty acid component was 15:1 iso H/13:0 3OH. Conclusions MS1721 strain belongs to the genus Bacillus, and Bacillus amyloliquefaciens MS1721 was proposed as the name for this strain on the basis of the taxonomic data. The second metabolite-macrolactin s produced by this strain exhibited obvious antibacterial activity.     

基因敲入小鼠模型鉴定及表型分析的研究进展

通过胚胎干细胞技术和DNA同源重组技术获得的基因敲入小鼠模型是国际上研究人类突变基因和类似疾病的趋势,可将整体动物实验与细胞水平研究结合,探索基因突变的发病机制和病理生理变化。而对该类模型的成功鉴定可为进一步的实验研究提供坚实的基础。本文就各种基因敲入小鼠模型的鉴定及表型分析工作进行综述。     

MRSA和鲍曼不动杆菌抗药基因检测及对3种醇类消毒剂抗性研究

目的观察耐甲氧西林金黄色葡萄球菌（MRSA）和鲍曼不动杆菌（ABA）对3种醇类消毒剂的抗性与耐药基因携带情况。方法选择最小抑菌浓度（MIC）试验、悬液定量杀菌试验和耐药基因PCR检测方法进行研究,并与标准菌株作平行比较检测。结果 2株MRSA对3种醇的MIC值高于6株鲍曼不动杆菌,稍高于标准菌株6538和8099。杀菌实验结果显示,3种醇对2株MRSA的杀灭对数值均低于标准株6538和8099,对MRSA-1杀灭对数值大于MRSA-2,对6株鲍曼不动杆菌的杀灭对数值大于标准株8099;作用至1 min时,3种醇均能将8099全部杀灭,对6株鲍曼不动杆菌的杀灭对数值D162〈3070122〈3070341〈3040217     

携带人血红素氧合酶-1基因重组腺病毒的构建及鉴定

目的:构建人血红素氧合酶 - 1(hHO - 1)重组腺病毒,为基因治疗心肌细胞缺氧性损伤提供可能.方法:将hHO - 1 cDNA克隆于腺病毒穿梭质粒pSGCMV,得到重组质粒pSGCMV - hHO - 1.采用位点特异性重组系统(Cre/Loxp)将pSGCMV - hHO - 1与腺病毒骨架载体pBHGloxP△E3通过Lipofectamine 2000共转染至293细胞,生成带有hHO - 1基因的重组腺病毒载体Ad - hHO - 1.重组腺病毒质粒在293细胞中扩增,氯化铯梯度离心纯化.结果:经酶切鉴定和测序证实载体构建的正确性,病毒滴度为2.0×1010pfu/ml.结论:成功构建带有hHO - 1 cDNA的重组腺病毒,可用于对心肌细胞缺氧性损伤的基因治疗研究.     

常见奈瑟菌(种)和卡他莫拉菌的生化鉴定及耐药性研究

 目的建立一套奈瑟菌(种)NC和卡他莫拉菌MC生化鉴定方法,了解NC和MC的耐药状况.方法对可疑菌株进行氧化酶试验、DNA酶试验、分解产酸试验,三汀精水解试验、β内酰胺酶试验,根据实验结果进行区分;采用琼脂平皿二倍稀释法测定最低抑菌浓度(MIC).结果 127株卡他莫拉菌中β内酰胺酶阳性率为94%,84株奈瑟菌中β内酰胺酶阳性率2.3%.卡他莫拉菌DNA酶试验、三汀精水解试验阳性率为100%.84株奈瑟菌中有2株对PIPC/SBT、PIPC/TAZ及CFP/SBT敏感,127株卡他莫拉菌中有120株对PIPC/SBT、PIPC/TAZ及CFP/SBT敏感.结论通过氧化酶试验、DNA酶试验、分解产酸试验、三汀精水解试验、β内酰胺酶试验,可将NC和MC一一区分开来;PIPC/SBT、PIPC/TAZ及CFP/SBT对β内酰胺酶阳性的NC和MC有较好的抗菌活性.     

表达人干扰素α-2b基因重组乳杆菌的鉴定及其抗病毒活性的动态研究

目的:进一步鉴定表达人干扰素α-2b的重组乳杆茵DM8909/pSC111AE-IFN-α-2b,考察重组茵的培养条件及其表达产物动态的抗病毒活性.方法:在LRS培养基培养重组菌株DM8909/pSC111AE-IFN-α-2b并诱导表达,用Western印迹鉴定培养上清中的表达产物,对重组菌株在LRS培养基中的不同时间的D600、pH值、活菌数、表达产物抗病毒活性进行动态观察.结果:Western印迹鉴定证实了重组茵上清中的IFN-α-2b.重组菌培养18 h后pH值由6.5开始下降,56 h后降至3.5;细菌于培养21 h进入对数期,32 h进入稳定期;培养24～32 h上清中干扰素抗病毒活性为4.08×105U/ml.结论:重组乳杆菌DM8909/pSC111AE-IFN-α-2b可表达IFN-α-2b于培养上清,培养24～32 h上清中干扰素含量最高,可用于预防及治疗病毒感染的进一步研究.