IMMUNOELECTRON MICROSCOPIC LOCALIZATION OFGROWTH FACTORS AND OTHER MARKERS IN HUMAN LONG-TERM BONE MARROW CULTURES

Ultrastructural immunocytochemical characterization of human long-term bone marrow cultures hasshown positive localization for growth factors on cell surface and on extracellular matrix (ECM). In somecases double-labelling indicated co-locallzation of growth factors and specific cell surface labels. Specific markers for endothelial cells and fibroblasts showed that growth factor (GM-CSF, G-CSF and b-FGF) were present at the surface of these cell types. Both scanning and transmision electron micrcscopy indicated intense labelling for growth factors on the extracellular matrix. Double-labelling of heparan sulphate proteoglycans and GM-CSF showed a co-localization of the labelling, which indicated thebinding of growth factor to the extracellular matrix.     

SEPARATON AND IDENTIFICATION OF IMMUNOSUPPRESSIVE FACTORS IN HUMAN SEMEN

The main function of immunosuppressive factors ( IFs ) in seminal plasma ( SP ) is the inhibition of immune response in genital tracts, thus the iso- and/ or autoimmune response to the spermatozoa and embryo are evaded. Studies on IFs in SP is thus important     

FIRST SUCCESSFUL AUTOTRANSPLANTATION OF BONE MARROW IN XIYUAN HOSPITAL

A good result in the combined TCM and WM treatment of leukemia was obtained in the Hematopathy Research Section of Xiyuan Hospital, China Academy of Traditional Chinese Medicine. The remission rate of first-treated patients reached more than 90%.     

EXPRESSION OF BONE MORPHOGENETIC PROTEIN-1 IN FETAL HUMAN CARTILAGE AND BONE DURING ENDOCHONDRAL OSSIFICATION

The expression ot mRNA for bone morphogenetie protein-l(BMP-1) was investigated in fetal human cartilage and bone during the developing endoehondral ossification by using in situ hybridization. BMP-1 mRNA was localized in the hypertrophic choadrocytes, the cells of perichondrium, osteoblasts and marrow cells. The results suggest that BMP-1 may involve in cartilage and bone formation, and in regulation of calcification of growth plate. As a comparative study, the expression of TGF-β1 protein was investigated in cartilage and bone using immunohistoehemical staining, correlated expression of TGF-β1 and BMP-1 was found. The results suggest that BMP-1 and TGF-β1 act synergistically to influence endochroodral ossification.     

EFFECTS OF XW630 ON BONE FORMATION IN OVARIECTOMIZED RATS AND IN HUMAN OSTEOBLAST-LIKE CELLS

Objective. To study the effects of XW630 on bone formation in overiectomized(OVX) rats and in hu-man osteoblast-like cell line TE85. Method. Bone histomorphometric analysis was performed with undecalcified bone sections and tetracy-cline intraperitoneally labeling. Results. Compared with that of OVX rats, the static data of trabecular bone volume(TBV)/total tis-sue volume(TTV), TBV/sponge bone volume(SBV) and mean trabecular plate density (MTPD) were en-hanced while mean trabecular plate spacing(MTPS) decreased after treated with XW630 for 13w. The dy-namic data of single-labeled surface [Sfract(s)], double-labeled surface[Sfract(d)],Sfract(d 1/2s),tra-becular osteoid surface(TOS), and bone formation rate in tissue level (Svf) were increased and osteoid maturation period (OMP) shortened in XW630 group. In osteoblast-like cells, both ^3H-thymidine incorpo-ration and cell count increased after treated with XW630 for 48. Treated with XW630 for 12～ 18h,in-ducible nitric oxide synthase(iNOS) activity and cGMP content increased in time-dependent manners. Conclusions. XW630 enhanced bone activation frequency and increased trabecular connectivity, stabili-ty, and strength. The cellular mechanism related to effects of XW630 on bone formation in ovariectomized     

EFFECTS OF TRANSFORMING GROWTH FACTOR β AND RECOMBINANT HUMAN BONE MORPHOGENETIC PROTEIN 2 ON HUMAN PERIODONTAL LIGAMENT FIBROBLASTS

R6SLnn Obetif Evaluer les effets de Wet de rkM sur as. mebo Faire la cul-ture Primaire de N et obServer les effets de dthentes concntrations de tal et de N sur laProlifdration de HPDLF, les activitis de Af, ta Syntha de I' osthalcine et Ia formtion des nduIesmithelifor. holfots W (5 -- 100ng/ml ) stimule de facon sghificative la Prolifhetion de HPtw.te activithe de Af sont nettement aewthe por 5ng/ml de tal. A 0, 5 -- 100ng/ml, tw n' a pesd' effet sur la optha de I' osMlcine et la f…     

HUMAN PAPILLOMAVIRUS IN CONDYLOMA ACCUMINATA AND OTHER BENIGN LESIONS OF THE FEMALE GENITAL TRACT

PCR was used to detect (HPV) in condyloma accuminata (CA),pseudocondyloma whose cause is unclear,and the other benign lesions in female genital tract.The results showed that HPV prevalence rate in CA was 98.2%,type 6 was in 11 was 85.7%,     

QUANTIFICATION AND LOCALIZATION OF WHEAT GERM AGGLUTININ RECEPTOR ON HUMAN SPERMME MBRANE IN FERTILE AND INFERTILE MALES

It has been proved/n our prcvious study that wheat germ agglutinin (WGA) receptor on human sperm membrane is closely related to male fertility and there exists a significant difference in WGA receptors betweea fertile and infertile mcn. In this report, enzyme linked     

LONG TERM PERFORMANCE OF STEROID-ELUTING LEAD IN DUAL CHAMBER PACING

The atrial pacing lead is believed having higher stimulation thresholds and long-term complicatinn rares than ventricular lead, this being one of the factors limiting the use of dual chamber pacing. A prospective study was undertaken to evaluate both atrial and ventricular bipolar tincd steroid-ehidng leade in long-term dunl chamber pacing. There are 81 pairs of leads (Medtronic Capsure SP) used in 81 patients.Bipolar atrial and ventrieular stimulation thresholds were measured immediately post implantatlon and 1,3,6,12,18 and 24 months. All leads demonstrated low mean stimulation thresholds during the follow-up and more than 94% of leads could be paced chronically in the atrium and ventricle at 2.5 volts. In conclusion,atrial and ventricular steroid-during leads gave excellent stimulation thresholds allowing low energy longterm dual chamber pacing.     

LONG-TERM EFFECTS OF THE G-CSF THERAPIES IN ALLOGENEIC BONE MARROW TRANSPLANTATION

Recently we have completed a 3-4 year follow-up of the 98 allogeneic bone marrow transplanted patients with various disorders.Thirty-eight had myeloid leukemis.Seventy-four cases received daily injections with lenograstim     

人骨髓细胞体外长期培养的建立

观察了由髂后上棘、胫骨穿刺抽吸的骨髓液及胸骨手术时刮取的骨髓液，以及在培养过程中不同时间用Ｆｉｃｏｌｌ－Ｈｙｐａｑｕｅ（ＦＨ）分离骨髓单个核细胞（ＭＮＣ）等因素对人骨髓细胞体外长期培养（Ｌｏｎｇ－ｔｅｒｍ ｈｕｍａｎ ｂｏｎｅ ｍａｒｒｏｗ ｃｕｌｔｕｒｅ，ＬＴＨＢＭＣ）的影响。结果显示；由刮取的骨髓液建立的培养，其基质层细胞生长、培养上清液中非粘附细胞及粒单细胞集落形成单位数目均优于抽吸者。原因     

人骨髓细胞体外长期培养基质层细胞的分类及其变化

人骨髓细胞体外长期培养基质层细胞按其核浆、形态特征分为四类：ＡＣ１最大，有数个明显核仁，胞浆呈薄纱状；ＡＣ２与巨噬细胞类似，核小深染，胞内有吞噬物；ＡＣ３最显著的特征是胞浆内广泛分布的脂质空泡；ＡＣ４最小，核大深染，胞浆少。从培养第４周至第８周，ＡＣ１及ＡＣ２分别从２１．７５％、３１．２５％上升至４０．５％、５５％；ＡＣ３及ＡＣ４分别从３１％、１５．５％降至１．５％、３％。碱性磷酸酶－抗碱性磷酸酶（ＡＰＡＡＰ）法检测细胞表面抗原，可见ＡＣ１纤维联结蛋白阳性，ＡＣ２的ＣＤ６８强阳性，ＡＣ３的纤维联结蛋白弱阳性，ＡＣ４的上两种单抗及因子ＶＩＩＩ相关活性抗原均标记阴性。本实验结果可作为一个参考值，衡量在各种实验条件下基质层细胞组成的变化。     

人βNGF cDNA探针制备及人骨髓组织中NGF基因表达

新近研究发现，神经生长因子对非神经系统也个有重要的功能效应。为探讨ＮＧＦ与骨髓造血组织的关系，本实验制备地高辛标记的人βＮＧＦｃＤＮＡ探针，通过原位杂交技术，检测正常人骨髓ＮＧＦｍＲＮＡ的分布。实验显示，βＮＧＦｃＤＮＡ探针与其互补的ｍＲＮＡ杂交反应信号存在于巨核细胞系和组织嗜碱细胞杂交后反应产物呈紫蓝色颗粒，分布于阳性细胞胞浆中。     

NGF基因在人骨髓巨核细胞表达的意义探讨

应用原位分子杂交技术，检测神经生长因子在人骨髓巨核细胞分化发育过程中的基因表达情况，以探讨ＮＧＦ对骨髓造血组织的功能意义。实验结果表明，βＮＧＦ ｃＤＮＡ探针与其互补的ｍＲＮＡ杂交反应信号存在于原巨核细胞，幼巨核细胞，颗粒型巨核细胞，成熟型巨核细胞等各级不同发育阶段的巨核细胞胞浆中，实验结果提示，ＮＧＦ在骨髓巨核细胞系的发育成熟过程中可能发挥调节作用。     

泰安市160例白血病的骨髓象及血象分析

对泰安市160例白血病的骨髓象及血象进行了统计分析,总结了骨髓增生程度、巨核细胞、血红蛋白、血小板、白细胞、血片中白血病细胞等在白血病时的变化特点,并对160例白血病进行了分类分型。     

异种脱钙骨基质诱导异位骨髓形成的实验研究

目的：开展诱导异位骨髓形成的研究,探讨骨髓形成及其调控机理方法：采用异种（人）脱钙骨基质粉,植入Ｓｐｒａｑｕｅ－Ｄａｗｌｅｙ大鼠腹壁两侧皮下,建立异种脱钙骨基质粉诱导异位骨髓形成的动物实验模型。结果：在诱导第７天可见间充质细胞包绕并迁入骨基质性植入物的间隙,分化成原始软骨细胞、形成软骨组织。诱导第１３天,新生软骨组织进一步增加,成骨细胞,破骨细胞和血管位于其中,呈现软骨化骨现象,类骨样基质产生,成     

粒单核细胞和粒细胞集落刺激因子在人胎盘滋养层细胞的定位

目的：确定脐血中高水平的造血因子的来源。方法：应用抗粒单核细胞集落刺激因子（ＧＭＣＳＦ）和抗粒细胞集落刺激因子（ＧＣＳＦ）的单克隆抗体对１９例健康产妇足月分娩的胎盘组织的冰冻切片进行免疫组织化学染色。以耳鼻咽喉科摘取的鼻息肉组织做对照。结果：胎盘郎汉斯细胞及合体细胞呈现阳性反应，而阴性对照无反应。结论：胎盘滋养层细胞可能是产生脐血中ＧＭＣＳＦ和ＧＣＳＦ的主要细胞     

骨髓局灶性残余白血病

3例急性淋巴细胞性白血病患者获得完全缓解(CR)后,外周骨髓仍存在未被抑制的局限性活性骨髓灶,骨髓细胞学检查表明常规穿刺部位的骨髓均呈CR髓像,但在局限性活性骨髓灶含有超过5%的幼淋白血病细胞,确定为骨髓残余白血病病灶,这与通常所指弥散分布于全身的白血病细胞而言的残余白血病系两个不同的概念。这是迄今为止首次发现的局限性骨髓残余病灶。这将对探讨白血病缓解的标准,制订治疗方案及判定预后具有意义。     

骨髓间充质干细胞向肝细胞的体外诱导分化

目的：探索肝细胞生长因子（HGF）、酸性成纤维细胞生长因子（aFGF）、制瘤素M（OSM）诱导骨髓间充质干细胞（MSCs）为肝细胞的能力及效果。方法：采用红细胞裂解＋贴壁法分离纯化大鼠骨髓MSCs,取第4代MSCs,按如下分组诱导其向肝细胞分化：①MSCs组（空白对照）：不加细胞因子;②H＋a组：20μg/LHGF＋20μg/LaFGF;③H＋O组：20μg/LHGF＋20μg/LOSM;④a＋O组：20μg/LaFGF＋20μg/LOSM;⑤H＋a＋O组：20μg/LHGF＋20μg/LaFGF＋20μg/LOSM;⑥H＋a＋O（分）组：20μg/LHGF（1～9d）＋20μg/LaFGF（9～18d）＋20μg/LOSM（9～21d）。于不同时间点采用RT-PCR检测甲胎蛋白（AFP）和细胞角蛋白18（CK18）的表达,溴甲酚绿法检测其中白蛋白（ALB）的含量,谷氨酸脱氢酶法检测其中尿素（Urea）含量。结果：除对照组外,其余各组在7～14d可见AFP mRNA的表达,21d仅a＋O组表达阳性。除对照组及a＋O组外,其余各组均出现CK18 mRNA、ALB及Urea,在同一时间点,H＋a＋O组表达量高于其余各组（P〈0.05）。结论：HGF联合aFGF/OSM能够成功诱导MSCs分化为肝细胞;aFGF与OSM不能诱导MSCs向较为成熟的肝细胞的分化,但与HGF联合使用能明显提高分化率。