Bronchodilator Activity of Aerial Parts of Polygonatum verticillatum Augmented by Anti‐inflammatory Activity: Attenuation of Ca2+ Channels and Lipoxygenase

Polygonatum verticillatum is commonly used for the treatment of asthma and inflammation. The current study was aimed to scrutinize the pharmacological profile of methanolic extract of the aerial parts (PA). Isolated tracheal preparations were used for the evaluation of bronchodilatory activity, whilst the in vivo carrageenan‐induced paw oedema test and an in vitro lipoxygenase (LOX) inhibitory assay were used for the assessment of the anti‐inflammatory profile of PA. When tested against carbachol and K⁺ (80 mM)‐induced contractions, PA caused complete inhibition of isolated rabbit tracheal preparations in a dose‐dependent mode, similar to verapamil. While elucidating possible mechanism, PA shifted the Ca²⁺ concentration–response curves to the right, analogous to that produced by verapamil, confirming a Ca²⁺ channel blocker‐like activity. PA provoked profound reduction in paw oedema with a maximum protection of 60.87% at 200 mg/kg i.p. in a dose‐dependent manner which was augmented by its prominent LOX inhibitory activity (IC₅₀: 125 µg/mL). These findings authenticated its therapeutic potential in the treatment of asthmatic and inflammatory conditions. Copyright © 2012 John Wiley & Sons, Ltd.     

龙葵总碱对肿瘤细胞膜钠泵及钙泵活性影响的研究

目的：观察龙葵总碱对S180小鼠及H22小鼠肿瘤细胞膜Na^＋，K^＋-ATPase及Ca^＋＋，Mg^＋＋-ATPase活性的影响。方法：荷瘤小鼠分为治疗组（高剂量、中剂量、低剂量）和对照组（生理盐水组、环磷酰胺组），分别测定各组肿瘤细胞膜的Na^＋，K^＋ ATPase及Ca^＋＋，Mg^＋＋ -ATPase活性。结果：龙葵总碱对S180小鼠及H22小鼠肿瘤细胞膜Na^＋，K^＋ -ATPase及Ca^＋＋，Mg^＋＋ -ATPase活性均有明显的抑制作用，并且其抑制作用呈量效正相关。结论：龙葵总碱这一作用可能是其抗肿瘤作用机理之一。关键词：龙葵总碱肿瘤细胞膜钠泵钙泵