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1.
B Poginsky J Westendorf B Bl?meke H Marquardt A Hewer P L Grover D H Phillips 《Carcinogenesis》1991,12(7):1265-1271
The naturally-occurring anthraquinones (AQs), alizarin (1,2-dihydroxyanthraquinone) and lucidin (1,3-dihydroxy-2-hydroxymethylanthraquinone), were incubated with DNA in the presence of S9 mix. The isolated DNA was analysed by 32P-postlabelling for the presence of aromatic adducts. Only lucidin formed up to five different DNA adducts in the range from 0.995 to 3.05 adducts/10(8) nucleotides. Lucidin was also incubated with polynucleotides poly[d(A-T)] and polydG*polydC in the presence of S9 mix. Analysis of polydG*polydC revealed a similar adduct pattern to that obtained with lucidin-modified DNA. Alizarin, lucidin, a glycoside mixture containing alizarinprimeveroside and lucidinprimeveroside, and Rubia Teep (a herbal drug made from Rubia tinctorum containing lucidin) were incubated with primary rat hepatocytes for 24 h and the isolated DNA was analysed by 32P-postlabelling. Lucidin, the glycoside mixture and Rubia Teep gave rise to DNA adducts, but alizarin did not. Male Parkes mice were treated orally for 4 days with alizarin (10 mg/d), lucidin (2 mg/d), the glycoside mixture (20 mg/d) or Rubia Teep (1/2 tablet/d) and DNA was isolated from liver, kidney, duodenum and colon. Analysis by 32P-postlabelling revealed that lucidin, the glycoside mixture and Rubia Teep, but not alizarin, formed DNA adducts in all the tissues examined but that the adduct patterns were organ-specific. 相似文献
2.
DNA adduct formation in the liver, pancreas, kidneys and uterus in ethynylestradiol (EE)-induced carcinogenesis and the effect of tamoxifen (TAM) on DNA adduct formation were evaluated in female Wistar JCL rats using the 32P-postlabeling method. Hyperplastic nodules were noted in the liver of all rats 4 months after the first oral administration of 0.075 mg of EE, and hepatocellular carcinoma was detected in 8.1% of rats treated with EE for 12 months. DNA adducts increased in the liver for 4 months, reaching a level of 7.3 adducts/10(7) nucleotides and decreasing thereafter. Formation of DNA adducts was also noted in the pancreas and kidney, but the adduct levels were lower than those in the liver. TAM inhibited estrogen receptors (ER) in liver tissues and completely suppressed the development of hyperplastic nodules or hepatocellular carcinoma but did not affect DNA adduct formation in the liver. In this model, therefore, EE is considered to cause mutations of hepatocytes due to DNA adduct formation without mediation by ER and to induce initiated cells to develop into hepatocellular carcinoma in the presence of ER-mediated hormonal activities. 相似文献
3.
DNA adduct formation in the liver, pancreas, kidneys and uterus in ethynylestradiol (EE)-induced carcinogenesis and the effect of tamoxifen (TAM) on DNA adduct formation were evaluated in female Wistar JCL rats using the 32 P-postlabeling method. Hyperplastic nodules were noted in the liver of all rats 4 months after the first oral administration of 0.075 mg of EE, and hepatocellular carcinoma was detected in 8.1% of rats treated with EE for 12 months. DNA adducts increased in the liver for 4 months, reaching a level of 7.3 adducts/107 nucleotides and decreasing thereafter. Formation of DNA adducts was also noted in the pancreas and kidney, but the adduct levels were lower than those in the liver. TAM inhibited estrogen receptors (ER) in liver tissues and completely suppressed the development of hyperplastic nodules or hepatocellular carcinoma but did not affect DNA adduct formation in the liver. In this model, therefore, EE is considered to cause mutations of hepatocytes due to DNA adduct formation without mediation by ER and to induce initiated cells to develop into hepatocellular carcinoma in the presence of ER-mediated hormonal activities. 相似文献
4.
K Takayama K Yamashita K Wakabayashi T Sugimura M Nagao 《Japanese journal of cancer research》1989,80(12):1145-1148
2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) is the most abundant mutagenic heterocyclic amine by weight in cooked foods. This mutagen was found to produce DNA adducts in all ten tested organs of rats using the 32P-postlabeling method. The level of DNA adducts in the pancreas, kidney and liver increased dose-dependently and feeding time-dependently up to four weeks. When diet containing 0.05% PhIP was given to rats for four weeks, levels of PhIP-DNA adducts were relatively high in the lung, pancreas and heart, being around 20 per 10(7) nucleotides, and lowest in the liver, being 2.20 per 10(7) nucleotides. Thus, PhIP showed a unique feature in the formation of DNA adducts compared to other mutagenic and carcinogenic heterocyclic amines, which produce the highest level of DNA adducts in the liver. 相似文献
5.
Chronic administration of estrogens to male Syrian hamsters induces kidney tumors. Co-administration of estrogen plus alpha-naphthoflavone (ANF) suppresses this kidney carcinogenesis but induces liver tumors instead. In an attempt to elucidate the mechanism of the switch from estrogen-induced kidney to liver carcinogenesis in response to ANF treatment, patterns of kidney and liver DNA adducts were investigated by 32P-postlabeling analysis and compared to controls. Chronic treatment of hamsters with ANF alone or in combination with estradiol resulted in a flavone-specific DNA adduct pattern in livers of these animals. These spots, adducts 1 and 2, on 32P-postlabeling maps were taken as evidence of covalent ANF-DNA modifications. The kidney-specific estrogen-induced indirect DNA adducts, observed previously in hamsters treated chronically with estrogen, occurred in renal but not hepatic DNA of animals treated with estradiol alone or in combination with ANF. Pretreatment of hamsters with ANF for 3 days decreased by 75-80% the hepatic and renal diethylstilbestrol (DES)-DNA adducts, which are formed after injection of a single large dose of DES. It is concluded from these changes in DNA adduct patterns and levels that estrogen quinone-DNA adduction may play an etiological role in estrogen-induced kidney cancer. The prevention of estrogen-induced kidney tumors by ANF co-treatment may be a consequence of the decrease in renal concentrations of these adducts in response to ANF. Hepatic concentrations of estrogen quinone-DNA adducts also decrease, but ANF-DNA adducts, observed only in liver, may assume an etiological role in the induction of hepatomas. 相似文献
6.
Frank A. Blommaert Charulla Michael H. C. M. van Dijk-Knijnenburg J. H. Schornagel Leo den Engelse A. M. J. Fichtinger-Schepman 《Cancer chemotherapy and pharmacology》1996,38(3):273-280
The formation and persistence of platinum-DNA adducts were studied with immuno(cyto)chemical methods in male and female Sprague-Dawley
rats treated with a single i.p. dose of carboplatin. Linear dose-effect curves were observed for kidney and liver with an
immunocytochemical assay using NKI-A59 antiserum that recognizes intrastrand cross-links. With this method, no staining of
the nuclei due to platinum-DNA damage could be observed in the spleen, testis, uterus, or ovary after administration of up
to 80 mg/kg carboplatin. A homogeneous staining of the nuclei in the liver was observed. The nuclear staining in the kidney
was somewhat more intense but less homogeneous, with small groups of intensely stained nuclei occasionally being seen in the
outer cortex. An approximately 15 to 20-times lower dose of cisplatin than of carboplatin was needed to reach equal staining
levels in the liver and kidney. Plateau staining levels in both tissues were reached at between approximately 8 and 48 h after
administration of the carboplatin. This was followed by a significant reduction in the kidney samples, whereas the staining
levels in the liver section seemed to be more persistent. No major difference was observed between male and female rats in
the formation and removal of DNA damage in these tissues. The levels of the various DNA adducts were measured with a competitive
ELISA in liver, kidney, spleen, testis, and combined ovary/uterus samples collected at 8 and 48 h after carboplatin administration.
At both 8 and 48 h, the highest platination levels were observed in the kidney, followed—in decreasing order—by the liver,
combined uterus and ovary samples, spleen, and testis. At 8 h after administration of carboplatin, the relative occurrence
of the bifunctional adducts Pt-GG (34%), Pt-AG (27%), and G-Pt-G (32%), was similar in all tissues. The same held for the
monoadducts that amounted to about 7% of the total DNA platination. These data indicate that in the first few hours after
carboplatin treatment, no preference for the formation of Pt-GG adducts was observed, which confirms our earlier observations
obtained with cultured cells. When the total DNA-platination levels (calculated from the sum of the adducts) seen at 8 and
48 h after treatment were compared, a substantial decrease in DNA platination was observed in the kidney (37%), liver (30%)
and ovary/uterus (39%), whereas the repair levels in the testis (9%) and, probably, the spleen (18%) were substantially lower.
In all tissues studied, only the relative occurrence of the Pt-GG adducts increased between 8 and 48 h, and as a result, at
48 h, after carboplatin administration the Pt-GG adduct was the major adduct persisting in the DNA samples.
Received: 31 May 1995/Accepted: 19 October 1995 相似文献
7.
Kyoko Takayama Katsumi Yamashita Keiji Wakabayashi Takashi Sugimura Minako Nagao 《Cancer science》1989,80(12):1145-1148
2-Amino-l-methyl-6-pheny]imidazo[4,5- b ]pyridine (PhIP) is the most abundant mutagenic heterocyclic amine by weight in cooked foods. This mutagen was found to produce DNA adducts in all ten tested organs of rats using the 32 P-postlabeling method. The level of DNA adducts in the pancreas, kidney and liver increased dose-dependently and feeding tinie-dependently up to four weeks. When diet containing 0.05% PhIP was given to rats for four weeks, levels of PhIP-DNA adducts were relatively high in the lung, pancreas and heart, being around 20 per 107 nucleotides, and lowest in the liver, being 2.20 per 107 nucleotides. Thus, PhIP showed a unique feature in the formation of DNA adducts compared to other mutagenic and carcinogenic heterocyclic amines, which produce the highest level of DNA adducts in the liver. 相似文献
8.
Stiborová M Breuer A Aimová D Stiborová-Rupertová M Wiessler M Frei E 《International journal of cancer. Journal international du cancer》2003,107(6):885-890
Ellipticine is a potent antineoplastic agent whose mode of action is considered to be based mainly on DNA intercalation and/or inhibition of topoisomerase II. Recently, we found that ellipticine also forms covalent DNA adducts in vitro and that the formation of the major adduct is dependent on the activation of ellipticine by cytochrome P450 (CYP). Here, we investigated the capacity of ellipticine to form DNA adducts in vivo. Male Wistar rats were treated with ellipticine, and DNA from various organs was analyzed by (32)P postlabeling. Ellipticine-specific DNA adduct patterns, similar to those found in vitro, were detected in most test organs. Only DNA of testes was free of the ellipticine-DNA adducts. The highest level of DNA adducts was found in liver (19.7 adducts per 10(7) nucleotides), followed by spleen, lung, kidney, heart and brain. One major and one minor ellipticine-DNA adducts were found in DNA of all these organs of rats exposed to ellipticine. Besides these, 2 or 3 additional adducts were detected in DNA of liver, kidney, lung and heart. The predominant adduct formed in rat tissues in vivo was identical to the deoxyguanosine adduct generated in DNA by ellipticine in vitro as shown by cochromatography in 2 independent systems. Correlation studies showed that the formation of this major DNA adduct in vivo is mediated by CYP3A1- and CYP1A-dependent reactions. The results presented here are the first report showing the formation of CYP-mediated covalent DNA adducts by ellipticine in vivo and confirm the formation of covalent DNA adducts as a new mode of ellipticine action. 相似文献
9.
Target organ-specific covalent DNA damage preceding diethylstilbestrol-induced carcinogenesis 总被引:1,自引:0,他引:1
The synthetic estrogen diethylstilbestrol (DES), a known humancarcinogen, induces renal carcinoma in male Syrian hamsterswithin 6 months after s.c. implantation. Tumor formation couldbe evoked by its hormonal properties or by a reactive genotoxicmetabolite binding to DNA, but previous attempts to detect adductshave failed. In the present study, kidney DNA of male Syrianhamsters, treated with s.c. DES implants to induce renal carcinoma,was analyzed for the presence of DESinduced adducts using 32P-postlabelingassay. Covalently-modified DNA nucleotides were detected inthe kidneys after chronic DES treatment, but not in kidneysof untreated hamsters, or in liver or tumor tissue of DEStreatedanimals. This report demonstrates for the first time the abilityof an estrogen to give rise to covalent DNA modification invivo specifically in the target organ of carcinogenesis. DES-inducedcovalent DNA adducts are taken as evidence for tumor initiationby DES via damage to cellular macromolecules, in addition totumor-promotional effects described previously. 相似文献
10.
Bartsch H Phillips DH Nair J Hewer A Meyberg-Solomeyer G Grischke EM 《Carcinogenesis》2000,21(4):845-847
Tamoxifen (TAM) is used for the adjuvant treatment of women with breast cancer and has also been recommended as a chemopreventive agent. Among unwanted side effects, TAM was shown to increase endometrial cancer in treated women by mechanisms that are not yet clearly understood. We studied DNA adducts in lymphocytes of female breast cancer patients treated with TAM or toremifene (TOR), a TAM analogue and compared them with adducts formed by TAM in rat liver, where the drug induces tumours. DNA adducts were measured by TLC-(32)P-post-labelling assays. After TLC, all DNA samples including DNA from untreated healthy women showed a faint radioactive zone, where the positive control DNA adducts isolated from the liver of rats treated with TAM migrated. The relative adduct levels were calculated from the radioactivity present in this zone. Means +/- SD of adduct levels per 10(8) nucleotides (associated with this area) were for untreated volunteers (control) 1.83 +/- 1.41 (n = 13), for TAM treatment 2.17 +/- 3.04 (n = 25) and for TOR treatment 1.18 +/- 1.05 (n = 8). Most of the human samples were further analysed by HPLC after labelling with (32)P in order to compare adducts in human DNA with those in liver DNA isolated from TAM-treated rats. None of the human samples showed any peaks at retention times where putative TAM-DNA adducts were eluted. In conclusion, lymphocyte DNA from female patients treated at therapeutic levels did not show evidence of the formation of TAM- or TOR-DNA adducts. 相似文献
11.
DNA adducts formed by 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline in rat liver: dose-response on chronic administration 总被引:6,自引:0,他引:6
K Yamashita M Adachi S Kato H Nakagama M Ochiai K Wakabayashi S Sato M Nagao T Sugimura 《Japanese journal of cancer research》1990,81(5):470-476
The effect of administration of 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx) at various doses on DNA adduct formation in male rats was examined by 32P-postlabeling analysis. Administration of MeIQx in the diet at 0.4 ppm, 4 ppm, 40 ppm and 400 ppm for one week resulted in the formations of 0.04, 0.28, 3.34 and 39.0 adducts per 10(7) nucleotides in rat liver cells. Continuous administration of 400 ppm of MeIQx in the diet for 61 weeks to rats induced hepatocellular carcinomas in all rats. The carcinogenicity of MeIQx at doses of 40 ppm or less is not known yet, but the above results show a linear relationship between the level of MeIQx administered and the adduct level. In rats treated with low doses of 0.4, 4 and 40 ppm of MeIQx, adduct levels increased linearly with time of treatment, the levels in week 12 being two to three times those in week 1. In contrast, on treatment with 400 ppm of MeIQx, the adduct level in the liver increased until week 4, when it was 110 adducts per 10(7) nucleotides, and then remained constant for the next 8 weeks. Induction of the multidrug-resistance gene was suggested to be involved in development of this plateau level. 相似文献
12.
Covalent modifications of DNA in various tissues of mice with harman or norharman were analyzed by 32P-postlabeling assay. Administration of 0.1% harman to mice in their diet for 4 weeks resulted in DNA adducts in the liver and kidney. No specific DNA adduct was detected in other tissues, such as the glandular stomach, large intestine and brain. Similar treatment of mice with norharman resulted in DNA adducts in the kidney, glandular stomach and large intestine, but not in the liver or brain. These results suggests the in vivo genotoxicities of harman and norharman. 相似文献
13.
14.
Tamoxifen is a liver carcinogen in rats and has been associated with an increased risk of endometrial cancer in women. Recent reports of DNA adducts in leukocyte and endometrial samples from women treated with tamoxifen suggest that it may be genotoxic to humans. One of the proposed pathways for the metabolic activation of tamoxifen involves oxidation to 4-hydroxytamoxifen, which may be further oxidized to an electrophilic quinone methide. In the present study, we compared the extent of DNA adduct formation in female Sprague-Dawley rats treated by gavage with seven daily doses of 54 micromol/kg tamoxifen or 4-hydroxytamoxifen and killed 24 h after the last dose. Liver weights and microsomal rates of ethoxyresorufin O-deethylation, 4-dimethylaminopyrine N-demethylation and p-nitrophenol oxidation were not altered by tamoxifen or 4-hydroxytamoxifen treatment. Uterine weights were decreased significantly and uterine peroxidase activity was decreased marginally in treated as compared with control rats. DNA adducts were assayed by 32P-post-labeling in combination with HPLC. Two major DNA adducts were detected in liver DNA from rats administered tamoxifen. These adducts had retention times comparable with those obtained from in vitro reactions of alpha-acetoxytamoxifen and 4-hydroxytamoxifen quinone methide with DNA. Hepatic DNA adduct levels in rats administered 4-hydroxytamoxifen did not differ from those observed in control rats. Likewise, adduct levels in uterus DNA from rats treated with tamoxifen or 4-hydroxytamoxifen were not different from those detected in control rats. These data suggest that a metabolic pathway involving 4-hydroxytamoxifen is not a major pathway in the activation of tamoxifen to a DNA-binding derivative in Sprague-Dawley rats. 相似文献
15.
Katsumi Yamashita Masaaki Adachi Shunji Kato Hitoshi Nakagama Masako Ochiai Keiji Wakabayashi Shigeaki Sato Minako Nagao Takashi Sugimura 《Cancer science》1990,81(5):470-476
The effect of administration of 2-amino-3,8-dimethylimidazo[4,5- f ]quinoxaline (MeIQx) at various doses on DNA adduct formation in male rats was examined by 32 P-postlabeling analysis. Administration of MeIQx in the diet at 0.4 ppm, 4 ppm, 40 ppm and 400 ppm for one week resulted in the formations of 0.04, 0.28, 3.34 and 39.0 adducts per 107 nucleotides in rat liver cells. Continuous administration of 400 ppm of MeIQx in the diet for 61 weeks to rats induced hepatocellular carcinomas in all rats. The carcinogenicity of MeIQx at doses of 40 ppm or less is not known yet, but the above results show a linear relationship between the level of MeIQx administered and the adduct level. In rats treated with low doses of 0.4, 4 and 40 ppm of MeIQx, adduct levels increased linearly with time of treatment, the levels in week 12 being two to three times those in week 1. In contrast, on treatment with 400 ppm of MeIQx, the adduct level in the liver increased until week 4, when it was 110 adducts per 107 nucleotides, and then remained constant for the next 8 weeks. Induction of the multidrug-resistance gene was suggested to be involved in development of this plateau level. 相似文献
16.
cis-Diamminedichloroplatinum (II)-DNA adduct formation in renal, gonadal, and tumor tissues of male and female rats 总被引:1,自引:0,他引:1
cis-Diamminedichloroplatinum(II) (cisplatin), a potent anticancer agent, is thought to exert its cytotoxic effects through DNA damage. Using a polyclonal rabbit antisera which recognizes intrastrand bidentate deoxy(ApG)- and deoxy(GpG)-N7-diammineplatinum adducts, an enzyme-linked immunosorbent assay has been developed to quantitate this adduct in cisplatin-exposed DNA. Cisplatin-DNA adducts were measured in renal, gonadal, and tumor (sarcoma) tissues of Sprague-Dawley rats following i.v. or i.p. administration of cisplatin. When drug was administered i.v. to animals fed ad libitum adduct levels were highest in kidneys, 50% lower in s.c. sarcoma, and substantially lower in gonads. Under these experimental conditions, a large interindividual variability in adduct formation was observed in renal and tumor tissues, and adduct levels in some samples were too low to measure. Higher values among individuals were obtained using tissues of animals fasted overnight and treated i.p. Adduct levels following i.p. injections of drug were higher in kidneys and gonads of male rats than in kidneys and gonads of female rats. Analysis of tissue platinum content demonstrated higher platinum levels in kidneys of male rats than in kidneys of female rats, but the magnitude of this gender difference in total tissue platinum was not as great as that observed for adduct formation. When the influence of castration on adduct formation was investigated, adduct levels in kidneys of castrated females were higher than those in sham-operated females, but adduct levels in kidneys of the castrated male animals were not substantively different from those seen in sham-operated male controls. We conclude that the route of drug administration, diet, and hormonal status of the animal are factors that may influence cisplatin-DNA adduct formation in the rat. 相似文献
17.
Accumulation and persistence of DNA adducts of the synthetic steroid cyproterone acetate in rat liver 总被引:2,自引:2,他引:0
Cyproterone acetate (CPA) is a synthetic steroid which is widelyused in antlandrogenic and gestagenic drugs. We have recentlyshown that CPA induces DNA adducts in cultured rat hepatocytesand in rat liver (1). In the present investigation, we studiedthe persistence and accumulation of CPA-derived DNA adductsin the liver of rats using the 32 technique. To study the persistenceof CPA-DNA adducts, rats were treated with a single oral doseof 10 (female rats) or 100 mg CPA/kg body wt (male rats). FourDNA adducts were detected in the liver of both gender. In femalerats, maximal total DNA adduct levels of 3.40±0.04 adducts/106nucleotides were observed after 1 week. Eleven weeks later,40% of the adducts determined after 1 week were still detectable.In male rats, maximal hepatic DNA adduct levels of 相似文献
18.
Bagnyukova TV Tryndyak VP Montgomery B Churchwell MI Karpf AR James SR Muskhelishvili L Beland FA Pogribny IP 《Carcinogenesis》2008,29(3):638-646
Genotoxic carcinogens, including 2-acetylaminofluorene (2-AAF), in addition to exerting their genotoxic effects, often cause a variety of non-genotoxic alterations in cells. It is believed that these non-genotoxic effects may be indispensable events in tumorigenesis; however, there is insufficient knowledge to clarify the role of carcinogens in both the genetic and epigenetic changes in premalignant tissues and a lack of conclusive information on the link between epigenetic alterations and carcinogenic exposure. In the current study, we investigated whether or not the mechanism of 2-AAF-induced hepatocarcinogenesis consists of both genotoxic (genetic) and non-genotoxic (epigenetic) alterations. Male and female Sprague-Dawley rats were fed NIH-31 diet containing 0.02% of 2-AAF for 6, 12, 18 or 24 weeks. The levels of DNA adducts obtained from 2-AAF in liver and kidney tissues were assessed by high-performance liquid chromatography combined with electrospray tandem mass spectrometry (HPLC-ES-MS/MS). N-(Deoxyguanosine-8-yl)-2-aminofluorene was the major adduct detected at all time points in both tissues. Global DNA methylation in the livers and kidneys, as determined by an HpaII-based cytosine extension assay and by HPLC-ES-MS/MS, did not change over the 24-week period. In the livers of male rats, there was a progressive decrease of global and long interspersed nucleotide element-1-associated histone H4 lysine 20 trimethylation, as well as hypermethylation of the p16(INK4A) gene. These epigenetic changes were not observed in the livers of female rats or the kidneys of both sexes. Importantly, morphological evidence of formation and progression of neoplastic process was observed in the liver of male rats only. In conclusion, we have demonstrated that exposure of rats to genotoxic hepatocarcinogen 2-AAF, in addition to formation of 2-AAF-specific DNA lesions, resulted in substantial alterations in cellular epigenetic status. 相似文献
19.
Early formation of DNA adducts compared with tumor formation in a long-term tumor study in rats after administration of 2-nitrofluorene 总被引:4,自引:2,他引:2
Cui Xian-Shu; Torndal Ulla-Britta; Eriksson Lennart C.; Mller Lennart 《Carcinogenesis》1995,16(9):2135-2141
2-Nitrofluorene (NF) is a model compound for nitroarenes whichhas been identified in diesel exhaust and in urban air. Thecurrent study was carried out to observe the carcinogenicityof different doses of NF to rats and DNA adduct formation indifferent organs at an early stage of NF administration. Onegroup of rats was fed basal diet as a control, whereas the otherthree groups of rats were fed basal diet supplemented with differentamounts of NF (0.24, 0.95 and 2.37 mmol NF/kg diet, referredto as low, medium and high dose, respectively). The rats wereexposed to NF continuously for 11 months, after which all groupsof rats were fed basal diet without NF for another 13 months.In the high dose group hepatocellular carcinomas were foundin all rats (20/20), forestomach squamous carcinomas in 11 andcortical kidney carcinomas in 10 rats. Fifteen out of 19 ratsfed the medium dose of NF had hepatocellular carcinomas, 16had forestomach squamous carcinomas and 15 had cortical kidneycarcinomas. The major tumors of the rats fed the low dose ofNF were forestomach squamous carcinomas (10/18). DNA adductsformed in tumor target organs after 1, 2, 6 and 10 days NF administrationwere dose- and time-dependent. Ten days after the start of NFadministration DNA adduct levels were found to be 54, 11 and6 DNA adducts/108 normal nucleotides in forestomach, liver andkidney respectively. In the non-tumor target organs levels inthe range 1.74.8 DNA adducts/108 normal nucleotides werefound. DNA adduct formation in this study showed a good correlationwith the localization of tumors, although there is a need foradditional factors for tumor formation. The results indicatethat DNA adduct formation is an important factor for tumor formationand suggest that DNA adducts could be used as biomarkers forgenotoxic risk. 相似文献
20.
I-compounds are age-related bulky DNA modifications that aredetected in untreated animals by 32P-postlabeling. To characterizetheir properties, I-compounds were compared with carcinogen-DNAadducts in liver, kidney and skin of three rodent species. Weanlingfemale Sprague-Dawley rats, ICR mice and Syrian hamsters werefed Tekiad LM485 chow diet for 3 months and raised concurrentlyand strictly under the same environmental conditions. Animalsof each species were treated topically with 24 µmol/kgdibenz [,j]acridine per day for 3 days, then by gavage oncewith a mixture of safrole and 7,12-dlmethylbenz[]anthracene(60 and 80 µmol/kg respectively), or with one of the individualcarcinogens, Liver, kidney and skin DNA from carcinogen-exposed(24 h after treatment) and unexposed animals was analyzed bythe monophosphate version of the 32P-postlabeling assay. Whileeach of the three carcinogens produced qualitatively identicalmajor adduct patterns in all samples examined, I-compounds inuntreated animals showed distinct species- and tissue- dependentprofiles. Rats displayed the highest I-compound levels but thelowest adduct levels in both liver and kidney among the threespecies. These findings demonstrate fundamental differencesbetween I-compounds and carcino gen-DNA adducts, and supportthe hypothesis that I-compound formation is primarily relatedto species-specific, i.e. genetically determined, normal metabolicactivities rather than exposure to environmental genotoxic carcinogens. 相似文献